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The map label for this gene is hipO [H]

Identifier: 99080559

GI number: 99080559

Start: 775182

End: 776354

Strand: Direct

Name: hipO [H]

Synonym: TM1040_0718

Alternate gene names: 99080559

Gene position: 775182-776354 (Clockwise)

Preceding gene: 99080558

Following gene: 99080560

Centisome position: 24.22

GC content: 60.87

Gene sequence:

>1173_bases
ATGCCCGTTCGGAATCGCTTTGCCGAGATGCTGCCCGAAATCACCGCTTGGCGTCATGATTTTCACGAACATCCCGAGTT
GATGTATGACGTGCATCGCACCGCCGGACTGGTTCAGGAGCGCCTCAATGCCTTTGGCTGTGACGAGGTAGTGCCGGGTA
TCGGCAAGACCGGTGTTGTCGGCGTCATCAAGGGCAAGAGCACCGCATCGGGCAAGGTGATCGCCCTGCGTGCCGACATG
GATGCACTGCCGATCCACGAGGAAACAGGCCTAGATTATGCGTCCAAGACGCCTGGCAAAATGCATGCCTGTGGTCATGA
CGGCCACACCGCGATCCTCTTGGGCGCTGCAAAATACCTCTCCGAGACGCGCAACTTTGATGGCACGGTGGTTCTGCTGT
TCCAGCCCGCAGAGGAAGGTGGGGCCGGAGGCAAGGCGATGGTCGACGATGGGGTGATGGATCGTTGGGGCGTCCAGGAG
GTTTACGGGTTGCACAATATGCCCGGCCTGCCAATGGGCCATATCGCGACCCGTGTTGGCGGGCTCTTGGCCTCTTCGGA
CGAGTTCGAGATCGAAGTGACCGGCAAGGGCGGCCACGCCGCGGCACCGCATGACGCGATCGACACCACGCTAGTGGCCT
CGCAGATCGTGGTCTCGCTTCACTCTATCGTGTCGCGCAACATCGATCCCATTCATCGCGTGGTGCTGACCGTCGGCACA
TTTGAGACCGACAGCACGGCGTCGAATGTGATCGCCCATCGCGCCAAGCTCAAAGGCACGGTACGCACGCTCGACTCCGA
AAACCGCGCCTTGGCAGAAGAGCGCGTGCGTCGCGTGGCCGAAGATACCGCCTCGGCTTTCGGGGCGAATGCCACGGTCA
AATGGACCCCAGGTTATCCGGTCACGGAAAACACCGCCCAAGAGGTTGAATATGCGGTGGAGGCCGCCAGAGCAGTTGCC
GAGAAAGTCGACCCTGATGCGGCGCCCATCATGCCGTCGGAGGATTTCGCCTACATGCTTGAAGCGCGCCCCGGCGCCTA
TATCTTCTTGGGCAATGGCGACACGGCCATGTGTCATCACCCGGCCTATCAGTTCGACGACTGCGCCATCCCGCTTGGGT
GCAGCTGGTTTGTCGAGCTTTGTGAACGCCGCATGCCCGCCAGCTCATCATAA

Upstream 100 bases:

>100_bases
TGCATGGGGCATGGCACCTGTTGGTTCACTTGTGCAACAGCCCTTGCGTTCCGGCCCCCCATGCGGCACTCATTTCGCTA
ACACGTTAAGGAGAGAGCTC

Downstream 100 bases:

>100_bases
GGGCGCTGCGTTGCAGCCCCTGTGCACCCGGCCGCTCCCCGTGCCGGGTGTGACCATCCGAAAGACAAGGAGAAAGACCT
ATGCCCGTCAAGAACCGCTT

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPVRNRFAEMLPEITAWRHDFHEHPELMYDVHRTAGLVQERLNAFGCDEVVPGIGKTGVVGVIKGKSTASGKVIALRADM
DALPIHEETGLDYASKTPGKMHACGHDGHTAILLGAAKYLSETRNFDGTVVLLFQPAEEGGAGGKAMVDDGVMDRWGVQE
VYGLHNMPGLPMGHIATRVGGLLASSDEFEIEVTGKGGHAAAPHDAIDTTLVASQIVVSLHSIVSRNIDPIHRVVLTVGT
FETDSTASNVIAHRAKLKGTVRTLDSENRALAEERVRRVAEDTASAFGANATVKWTPGYPVTENTAQEVEYAVEAARAVA
EKVDPDAAPIMPSEDFAYMLEARPGAYIFLGNGDTAMCHHPAYQFDDCAIPLGCSWFVELCERRMPASSS

Sequences:

>Translated_390_residues
MPVRNRFAEMLPEITAWRHDFHEHPELMYDVHRTAGLVQERLNAFGCDEVVPGIGKTGVVGVIKGKSTASGKVIALRADM
DALPIHEETGLDYASKTPGKMHACGHDGHTAILLGAAKYLSETRNFDGTVVLLFQPAEEGGAGGKAMVDDGVMDRWGVQE
VYGLHNMPGLPMGHIATRVGGLLASSDEFEIEVTGKGGHAAAPHDAIDTTLVASQIVVSLHSIVSRNIDPIHRVVLTVGT
FETDSTASNVIAHRAKLKGTVRTLDSENRALAEERVRRVAEDTASAFGANATVKWTPGYPVTENTAQEVEYAVEAARAVA
EKVDPDAAPIMPSEDFAYMLEARPGAYIFLGNGDTAMCHHPAYQFDDCAIPLGCSWFVELCERRMPASSS
>Mature_389_residues
PVRNRFAEMLPEITAWRHDFHEHPELMYDVHRTAGLVQERLNAFGCDEVVPGIGKTGVVGVIKGKSTASGKVIALRADMD
ALPIHEETGLDYASKTPGKMHACGHDGHTAILLGAAKYLSETRNFDGTVVLLFQPAEEGGAGGKAMVDDGVMDRWGVQEV
YGLHNMPGLPMGHIATRVGGLLASSDEFEIEVTGKGGHAAAPHDAIDTTLVASQIVVSLHSIVSRNIDPIHRVVLTVGTF
ETDSTASNVIAHRAKLKGTVRTLDSENRALAEERVRRVAEDTASAFGANATVKWTPGYPVTENTAQEVEYAVEAARAVAE
KVDPDAAPIMPSEDFAYMLEARPGAYIFLGNGDTAMCHHPAYQFDDCAIPLGCSWFVELCERRMPASSS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=430, Percent_Identity=29.3023255813954, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41942; Mature: 41811

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRNRFAEMLPEITAWRHDFHEHPELMYDVHRTAGLVQERLNAFGCDEVVPGIGKTGVV
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEE
GVIKGKSTASGKVIALRADMDALPIHEETGLDYASKTPGKMHACGHDGHTAILLGAAKYL
EEEECCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHH
SETRNFDGTVVLLFQPAEEGGAGGKAMVDDGVMDRWGVQEVYGLHNMPGLPMGHIATRVG
HHHCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHH
GLLASSDEFEIEVTGKGGHAAAPHDAIDTTLVASQIVVSLHSIVSRNIDPIHRVVLTVGT
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
FETDSTASNVIAHRAKLKGTVRTLDSENRALAEERVRRVAEDTASAFGANATVKWTPGYP
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VTENTAQEVEYAVEAARAVAEKVDPDAAPIMPSEDFAYMLEARPGAYIFLGNGDTAMCHH
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEC
PAYQFDDCAIPLGCSWFVELCERRMPASSS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVRNRFAEMLPEITAWRHDFHEHPELMYDVHRTAGLVQERLNAFGCDEVVPGIGKTGVV
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEE
GVIKGKSTASGKVIALRADMDALPIHEETGLDYASKTPGKMHACGHDGHTAILLGAAKYL
EEEECCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHH
SETRNFDGTVVLLFQPAEEGGAGGKAMVDDGVMDRWGVQEVYGLHNMPGLPMGHIATRVG
HHHCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHH
GLLASSDEFEIEVTGKGGHAAAPHDAIDTTLVASQIVVSLHSIVSRNIDPIHRVVLTVGT
HHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
FETDSTASNVIAHRAKLKGTVRTLDSENRALAEERVRRVAEDTASAFGANATVKWTPGYP
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
VTENTAQEVEYAVEAARAVAEKVDPDAAPIMPSEDFAYMLEARPGAYIFLGNGDTAMCHH
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEC
PAYQFDDCAIPLGCSWFVELCERRMPASSS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]