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The map label for this gene is gatB [H]

Identifier: 99080542

GI number: 99080542

Start: 749516

End: 751024

Strand: Reverse

Name: gatB [H]

Synonym: TM1040_0701

Alternate gene names: 99080542

Gene position: 751024-749516 (Counterclockwise)

Preceding gene: 99080543

Following gene: 99080538

Centisome position: 23.46

GC content: 58.78

Gene sequence:

>1509_bases
ATGCTCGACCTGACATATGAACTCCCAAAGCCCAAGGTAATCGCGGGCGCCAAGCATGACTGGGAACTGGTCATCGGTAT
GGAGGTGCATGCCCAGGTCAGCTCCAATGCCAAGCTGTTTTCGGGCGCTTCCACCCAGTTTGGGGCGGAGCCCAACTCCA
ACGTGGCATTTGTGGATGCGGCCATGCCCGGCATGCTGCCCGTCATCAACGAATACTGCGTCGAACAGGCGGTCCGCACG
GGTCTTGGCCTCAAGGCGGATATCAACCTCTGGTCTGCCTTTGACCGCAAGAACTATTTTTACCCCGACCTGCCGCAGGG
CTATCAGATCTCCCAGCTCTATCACCCCATCGTGGGCGAAGGCGAAGTGCTGGTAGAGCTTGGCGATGGCACGGCGCGCA
TGGTGCGTATCGAGCGCATCCACATGGAGCAGGACGCGGGCAAATCCATCCACGATATGGACCCCTCCATGTCCTTTGTG
GACCTGAACCGGACCGGCGTCTGCCTGATGGAAATCGTCTCGCGCCCGGACATCCGTGGCCCCGAGGAAGCGGCCGCCTA
TATCGCCAAGCTGCGCCAGATCATGCGTTACCTTGGCACCTGTGACGGCAACATGCAGAACGGCAACCTGCGCGCGGATG
TGAACGTCTCCATCTGCCGCCCCGGCGCCTACGAGAAGTATCAGGAAACGCAGGATTTCTCGCATCTCGGCACCCGCTGC
GAGATCAAGAACATGAACTCCATGCGCTTTATCCAGCAGGCCATCGAGGTCGAAGCACGTCGCCAGATCGCCATTGTAGA
AGCCGGTGGCGAAGTCGAGCAGGAAACCCGCCTTTACGATCCAGACAAGGGCGAGACGCGTTCGATGCGTTCCAAGGAAG
AGGCGCATGACTATCGCTACTTCCCCGACCCGGACCTTCTGCCGCTCGAGATCGAGCAGGCTTGGGTAGACGACATCAAG
TCGAAGCTCCCCGAACTGCCGGACGAAAAGAAAGCCCGTTTTATCAAAGATTTCGGCCTTACAGACTATGATGCGTCCGT
GCTGACGGCGGATCTGGAGAGTGCGCATTATTTTGACGCCGTTGCAAAAGGTCGCAGCGGCAAGCTGGCGGCGAACTGGG
TCATCAACGAACTCTTTGGCCGCCTGAAGAAGGACGACAAAGACATCACTGACAGCCCCGTCTCCCCGGCGCAACTGGGT
GGTGTGATTGATCTGATCGCTTCTGACGCCATCTCCGGCAAGATCGCCAAGGATCTGTTTGAGATCGTTTATACCGAAGG
CGGAGACCCGGCCCAGATCGTCGAAGAACGTGGCATGAAGCAGGTCACAGACACCGGTGCGATTGAGGCGGCTCTGGACG
AGATCATCGCCGCCAACCCCGCACAGGTTGAAAAGGCCAAGGAAAACCCGAAGCTTGCGGGCTGGTTCGTCGGTCAGGTG
ATGAAAGCCACCGGCGGCAAGGCCAACCCGAAGGCGGTGAACCAGCTTGTAGCCAAGAAGCTTGGCTAA

Upstream 100 bases:

>100_bases
AAGGGCAAGAACACCGCTCGCCGCCATTGCCCCTTGCTCCTCGGCCTCTGCGCGCCTAGAACGCTTGGCAAACAAGGACA
TTCGGATACGGCGGAAAACC

Downstream 100 bases:

>100_bases
GCGTCCAGCCAGAACGTAAGACAAAAGAGGCGACCGCAAGGTCGCCTTTTTCACGTCAGAGACGGACGGTGATGCTTATT
CTGCCGCCAAGCCCCGTTCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTQFGAEPNSNVAFVDAAMPGMLPVINEYCVEQAVRT
GLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTARMVRIERIHMEQDAGKSIHDMDPSMSFV
DLNRTGVCLMEIVSRPDIRGPEEAAAYIAKLRQIMRYLGTCDGNMQNGNLRADVNVSICRPGAYEKYQETQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGEVEQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIK
SKLPELPDEKKARFIKDFGLTDYDASVLTADLESAHYFDAVAKGRSGKLAANWVINELFGRLKKDDKDITDSPVSPAQLG
GVIDLIASDAISGKIAKDLFEIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKENPKLAGWFVGQV
MKATGGKANPKAVNQLVAKKLG

Sequences:

>Translated_502_residues
MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTQFGAEPNSNVAFVDAAMPGMLPVINEYCVEQAVRT
GLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTARMVRIERIHMEQDAGKSIHDMDPSMSFV
DLNRTGVCLMEIVSRPDIRGPEEAAAYIAKLRQIMRYLGTCDGNMQNGNLRADVNVSICRPGAYEKYQETQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGEVEQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIK
SKLPELPDEKKARFIKDFGLTDYDASVLTADLESAHYFDAVAKGRSGKLAANWVINELFGRLKKDDKDITDSPVSPAQLG
GVIDLIASDAISGKIAKDLFEIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKENPKLAGWFVGQV
MKATGGKANPKAVNQLVAKKLG
>Mature_502_residues
MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTQFGAEPNSNVAFVDAAMPGMLPVINEYCVEQAVRT
GLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTARMVRIERIHMEQDAGKSIHDMDPSMSFV
DLNRTGVCLMEIVSRPDIRGPEEAAAYIAKLRQIMRYLGTCDGNMQNGNLRADVNVSICRPGAYEKYQETQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGEVEQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIK
SKLPELPDEKKARFIKDFGLTDYDASVLTADLESAHYFDAVAKGRSGKLAANWVINELFGRLKKDDKDITDSPVSPAQLG
GVIDLIASDAISGKIAKDLFEIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKENPKLAGWFVGQV
MKATGGKANPKAVNQLVAKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=507, Percent_Identity=40.2366863905325, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=326, Percent_Identity=38.3435582822086, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=459, Percent_Identity=32.244008714597, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI45550808, Length=511, Percent_Identity=34.6379647749511, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 55477; Mature: 55477

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTQFGAEPNSNVAFVDA
CCCCEECCCCCCEEECCCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEC
AMPGMLPVINEYCVEQAVRTGLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGE
CCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVELGDGTARMVRIERIHMEQDAGKSIHDMDPSMSFVDLNRTGVCLMEIVSRPDIRG
CEEEEEECCCCEEEEEEHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHCCCCCCC
PEEAAAYIAKLRQIMRYLGTCDGNMQNGNLRADVNVSICRPGAYEKYQETQDFSHLGTRC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGEVEQETRLYDPDKGETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCC
FPDPDLLPLEIEQAWVDDIKSKLPELPDEKKARFIKDFGLTDYDASVLTADLESAHYFDA
CCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHH
VAKGRSGKLAANWVINELFGRLKKDDKDITDSPVSPAQLGGVIDLIASDAISGKIAKDLF
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
EIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKENPKLAGWFVGQV
HHHHCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHH
MKATGGKANPKAVNQLVAKKLG
HHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTQFGAEPNSNVAFVDA
CCCCEECCCCCCEEECCCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEC
AMPGMLPVINEYCVEQAVRTGLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGE
CCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVELGDGTARMVRIERIHMEQDAGKSIHDMDPSMSFVDLNRTGVCLMEIVSRPDIRG
CEEEEEECCCCEEEEEEHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHCCCCCCC
PEEAAAYIAKLRQIMRYLGTCDGNMQNGNLRADVNVSICRPGAYEKYQETQDFSHLGTRC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGEVEQETRLYDPDKGETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCC
FPDPDLLPLEIEQAWVDDIKSKLPELPDEKKARFIKDFGLTDYDASVLTADLESAHYFDA
CCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHH
VAKGRSGKLAANWVINELFGRLKKDDKDITDSPVSPAQLGGVIDLIASDAISGKIAKDLF
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
EIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKENPKLAGWFVGQV
HHHHCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHH
MKATGGKANPKAVNQLVAKKLG
HHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA