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The map label for this gene is argF

Identifier: 99080507

GI number: 99080507

Start: 712251

End: 713177

Strand: Direct

Name: argF

Synonym: TM1040_0666

Alternate gene names: 99080507

Gene position: 712251-713177 (Clockwise)

Preceding gene: 99080506

Following gene: 99080511

Centisome position: 22.25

GC content: 59.22

Gene sequence:

>927_bases
ATGAACCATTTCCTCGATATCCATAAAACCGCACCCGAAGATCTGCGTCAGATCATTGATCAGGCCGGGCAAATGAAAAC
CGCACGTCTTGGCCGCCCCAAAGGCACGCCAGATGACGAGCAGCCGCTTGCAGGGCATATGGTTGCGCTGATCTTTGAAA
AGCCCTCGACCCGGACGCGGGTTTCTTTTGATGTCGGGGTGCGCCAGATGGGCGGCGAAACCATGGTGCTCTCCGGCAAG
GACATGCAGCTTGGTCACGGCGAGACCATCGCTGACACCGCGCGGGTGCTGTCGCGCTATGTGGACATGATCATGATCCG
GACTTTCGACGAAACCGTGCTTACCGAGATGGCAGAATATGCCGATGTGCCGGTGATCAACGGTCTTACCGACCGCACCC
ACCCCTGTCAGATCATGGCCGATATCCTCACCTATGAAGAACACCGCGGCCCCATCAAGGGCAAGAAGGTGGTCTGGACC
GGGGACGGAAACAATGTTTGCGCGTCTTTTCTCCACGCGGCGGGTCAGTTCGGCTTCGACCTTACCTTTACTGGTCCGGC
GCAGCTTGACCCGGAAGATGAATTCATCGGGTTTGCGCGCAACGCGGGCTCCAAGATCACTATCGAGCGGGATGCGGCGA
AGGCCGTTGAGGGCGCGGATCTGGTGGTGGCCGACACTTGGGTGTCGATGCATGACAGCCAATCCTCGAAGGAGCGGCGC
CACAACATGCTGCGTCCCTATCAGGTCAACGACGCGTTGATGGCGCATGCCAAGCCGGACGCGCTCTTTATGCATTGCCT
GCCAGCGCACCGCGAGGAAGAGGTGACCTCGGCAGTGATGGATGGCCCCCAGTCGGTGATTTTTGACGAAGCCGAAAATC
GCCTGCACGCGCAAAAGGCGGTCATGCGCTGGTGTCTGGGCGTCTGA

Upstream 100 bases:

>100_bases
GCCACTGCGGTCGAAGCCGCCTGACCTTCATCTTTTTACAAATAGCTCGGGGGTGCGGGGGCTGGCCCCCGCATGTCCCT
CTCCAGAAAGCGACGACAAC

Downstream 100 bases:

>100_bases
CGCTACACCACGGGCGCACTCCCGCCCGTCGGATGCAGTATTGAAATACTACCCCTCCCGTTGGGGATTGCGCCGGGCCT
TTGGCCCGGCGCTCGGCGTC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNHFLDIHKTAPEDLRQIIDQAGQMKTARLGRPKGTPDDEQPLAGHMVALIFEKPSTRTRVSFDVGVRQMGGETMVLSGK
DMQLGHGETIADTARVLSRYVDMIMIRTFDETVLTEMAEYADVPVINGLTDRTHPCQIMADILTYEEHRGPIKGKKVVWT
GDGNNVCASFLHAAGQFGFDLTFTGPAQLDPEDEFIGFARNAGSKITIERDAAKAVEGADLVVADTWVSMHDSQSSKERR
HNMLRPYQVNDALMAHAKPDALFMHCLPAHREEEVTSAVMDGPQSVIFDEAENRLHAQKAVMRWCLGV

Sequences:

>Translated_308_residues
MNHFLDIHKTAPEDLRQIIDQAGQMKTARLGRPKGTPDDEQPLAGHMVALIFEKPSTRTRVSFDVGVRQMGGETMVLSGK
DMQLGHGETIADTARVLSRYVDMIMIRTFDETVLTEMAEYADVPVINGLTDRTHPCQIMADILTYEEHRGPIKGKKVVWT
GDGNNVCASFLHAAGQFGFDLTFTGPAQLDPEDEFIGFARNAGSKITIERDAAKAVEGADLVVADTWVSMHDSQSSKERR
HNMLRPYQVNDALMAHAKPDALFMHCLPAHREEEVTSAVMDGPQSVIFDEAENRLHAQKAVMRWCLGV
>Mature_308_residues
MNHFLDIHKTAPEDLRQIIDQAGQMKTARLGRPKGTPDDEQPLAGHMVALIFEKPSTRTRVSFDVGVRQMGGETMVLSGK
DMQLGHGETIADTARVLSRYVDMIMIRTFDETVLTEMAEYADVPVINGLTDRTHPCQIMADILTYEEHRGPIKGKKVVWT
GDGNNVCASFLHAAGQFGFDLTFTGPAQLDPEDEFIGFARNAGSKITIERDAAKAVEGADLVVADTWVSMHDSQSSKERR
HNMLRPYQVNDALMAHAKPDALFMHCLPAHREEEVTSAVMDGPQSVIFDEAENRLHAQKAVMRWCLGV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=264, Percent_Identity=38.2575757575758, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI18105007, Length=269, Percent_Identity=26.3940520446097, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=38.9057750759878, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=38.622754491018, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=25.0783699059561, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994908, Length=345, Percent_Identity=24.6376811594203, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=288, Percent_Identity=23.2638888888889, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=311, Percent_Identity=32.475884244373, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=26.0188087774295, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24642586, Length=288, Percent_Identity=25.6944444444444, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_SILST (Q1GIW7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612661.1
- ProteinModelPortal:   Q1GIW7
- SMR:   Q1GIW7
- STRING:   Q1GIW7
- GeneID:   4077274
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0666
- NMPDR:   fig|292414.1.peg.2774
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q1GIW7
- ProtClustDB:   PRK00779
- BioCyc:   SSP292414:TM1040_0666-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34187; Mature: 34187

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 107-107 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHFLDIHKTAPEDLRQIIDQAGQMKTARLGRPKGTPDDEQPLAGHMVALIFEKPSTRTR
CCCCEECCCCCHHHHHHHHHHHCCEEHHHCCCCCCCCCCCCCHHHCEEEEEEECCCCCEE
VSFDVGVRQMGGETMVLSGKDMQLGHGETIADTARVLSRYVDMIMIRTFDETVLTEMAEY
EEEEHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVPVINGLTDRTHPCQIMADILTYEEHRGPIKGKKVVWTGDGNNVCASFLHAAGQFGFD
CCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCE
LTFTGPAQLDPEDEFIGFARNAGSKITIERDAAKAVEGADLVVADTWVSMHDSQSSKERR
EEECCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEEECHHHHCCCCHHHHHH
HNMLRPYQVNDALMAHAKPDALFMHCLPAHREEEVTSAVMDGPQSVIFDEAENRLHAQKA
HHCCCCEECCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VMRWCLGV
HHHHHHCC
>Mature Secondary Structure
MNHFLDIHKTAPEDLRQIIDQAGQMKTARLGRPKGTPDDEQPLAGHMVALIFEKPSTRTR
CCCCEECCCCCHHHHHHHHHHHCCEEHHHCCCCCCCCCCCCCHHHCEEEEEEECCCCCEE
VSFDVGVRQMGGETMVLSGKDMQLGHGETIADTARVLSRYVDMIMIRTFDETVLTEMAEY
EEEEHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADVPVINGLTDRTHPCQIMADILTYEEHRGPIKGKKVVWTGDGNNVCASFLHAAGQFGFD
CCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCE
LTFTGPAQLDPEDEFIGFARNAGSKITIERDAAKAVEGADLVVADTWVSMHDSQSSKERR
EEECCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEEECHHHHCCCCHHHHHH
HNMLRPYQVNDALMAHAKPDALFMHCLPAHREEEVTSAVMDGPQSVIFDEAENRLHAQKA
HHCCCCEECCCHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VMRWCLGV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA