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The map label for this gene is aspS

Identifier: 99080476

GI number: 99080476

Start: 677748

End: 679532

Strand: Direct

Name: aspS

Synonym: TM1040_0635

Alternate gene names: 99080476

Gene position: 677748-679532 (Clockwise)

Preceding gene: 99080475

Following gene: 99080478

Centisome position: 21.17

GC content: 60.0

Gene sequence:

>1785_bases
ATGCACGCATTTCGCAGCCATACCTGCGCTGAACTGAACAAATCCCACGTGGGCGAAACCGTTCGCCTCTCCGGCTGGGT
TCACCGGGTCCGCGATCACGGCGGCCTTTTGTTCATCGACCTGCGCGACCACTATGGCGTCACGCAAGTCATGGCCGACC
CCGACAGCCCTGTGTTTGCCGAGATTGAAAAGGTGCGGTCCGAATGGTGTATCCGCATCGATGGTGAAGTGAAGGCACGT
GACGAAAGCCTCGTGAACGCCAAGATCCCGACCGGCGAGATTGAGGTTTTCATCCGCGACATCGAAGTTCTGGGCAAATC
CGAAGAGCTGCCGCTGATGGTTTTCGGCGAGCAGGAATACCCGGAAGAAACCCGCTTGCGCTATCGCTACCTCGACCTGC
GCCGCGAGAAGATGCAGCGCAACATGGTGCTGCGCTCCAACATGATCCGCTCTATCCGCAATCGGATGTGGGATCAGGGT
TTCAACGAATACCAGACACCGATCATCACCGCGTCGAGCCCCGAAGGCGCGCGCGACTTCCTCGTGCCGTCCCGTCTGCA
CCCGGGCAAGTTCTACGCCCTGCCGCAGGCGCCGCAGCAGTTCAAACAGCTGATGATGGTGTCGGGCTTTGACAAGTATT
TCCAGATCGCGCCCTGTTTCCGCGACGAGGACCCTCGGGCGGACCGCTCGCCGACCGATTTCTACCAGCTCGATATGGAA
ATGTCCTTTGTCACCCAGCAGGATGTGTTTGACACCATCGCGCCGGTGATCGCGGGCGTGTTCGAGGAATTCGGTCAGGG
CCGCAAGGTCGATGCTCCGAATGAATGGCCGCAAATTTCCTATAAGGACGCGGCCCTCTGGTATGGCTCCGACAAGCCGG
ACCTGCGCAACCCGATCAAAATGCAGGTGGTGTCCGAGCATTTCCGCGGCTCTGGTTTTGCAATCTTTGCAAAGCTCCTG
GAGCAGGAAGGCACCGAAATTCGCGCCATTCCCGCACCGACAGGTGGCTCGCGCAAGTTCTGTGACCGGATGAATGCCTT
TGCCCAGAAAGAGGGCCTGCCGGGCATGGGCTATATCTTCTGGCGCGAGGGCGCGGACGGCATGGAAGCCGCTGGACCCT
TGGCGAAAAACATCGGCCCCGAACGCACCGAAGCGATCCGCCAGCAGCTTGGTCTGGGTGTGGGCGATGCGGCCTTCTTC
CTCGGGGGCAAGCCCAAGACATTCGAGGCGGTTGCAGGCCGCGCGCGCAATGTGATCGGCGAAGAACTGAACCTCACCGA
CAAGGACCGTTTCGCCTTTGCCTGGATCGTGGACTTCCCGATCTATGAAAAGGACGAAGAGACCGGCAAGATCGACTTTG
AGCACAACCCCTTCTCCATGCCGCAGGGCGGAATGGACGCGTTGAACGGCGATCCACTCGATGTGCGCGGCAACCAGTAT
GATCTGGCTTGTAACGGTTACGAGCTGGTCTCGGGCGCGATCCGGAACCACAAGCCAGAGATCATGTTCAAAGCCTTCGA
GATCGCAGGCTACGGCAAGGAAGAGGTCGAAAAGCGCTTTGGCGGTATGGTCAACGCGTTCCAGTATGGCGCCCCGCCGC
ACGGGGGCTGTGCAGCCGGTATCGATCGCATGGTGATGCTTCTGGCGGATGAGGCCAACATCCGCGAGGTCATCATGTTC
CCGATGAATCAGCGCGCCGAAGACCTGATGATGGCCGCACCCTCCGAGCCGATGTCGGAGCAGCTGATGGAACTTGGCCT
GCGCGTGATCCCGCAGGACGACTGA

Upstream 100 bases:

>100_bases
TTGACAACGGGGCGGGAACCTTGTGTACGGAAGGTTGAATTTTGCTTGGGCACGGCGCCCCCTCGCGCGCCCCCGCCGCT
GACCGAAAGAGACCAGATCC

Downstream 100 bases:

>100_bases
GACTGACGCATCGACGGATTATGATGGGGGCTGCCAGTTTGGCGGCCCCTTTTCTTTGGTCCGCGCGCGTTTCTGCCGAG
GCCCGTGCTGCGTCTTTCAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MHAFRSHTCAELNKSHVGETVRLSGWVHRVRDHGGLLFIDLRDHYGVTQVMADPDSPVFAEIEKVRSEWCIRIDGEVKAR
DESLVNAKIPTGEIEVFIRDIEVLGKSEELPLMVFGEQEYPEETRLRYRYLDLRREKMQRNMVLRSNMIRSIRNRMWDQG
FNEYQTPIITASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLMMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDME
MSFVTQQDVFDTIAPVIAGVFEEFGQGRKVDAPNEWPQISYKDAALWYGSDKPDLRNPIKMQVVSEHFRGSGFAIFAKLL
EQEGTEIRAIPAPTGGSRKFCDRMNAFAQKEGLPGMGYIFWREGADGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFF
LGGKPKTFEAVAGRARNVIGEELNLTDKDRFAFAWIVDFPIYEKDEETGKIDFEHNPFSMPQGGMDALNGDPLDVRGNQY
DLACNGYELVSGAIRNHKPEIMFKAFEIAGYGKEEVEKRFGGMVNAFQYGAPPHGGCAAGIDRMVMLLADEANIREVIMF
PMNQRAEDLMMAAPSEPMSEQLMELGLRVIPQDD

Sequences:

>Translated_594_residues
MHAFRSHTCAELNKSHVGETVRLSGWVHRVRDHGGLLFIDLRDHYGVTQVMADPDSPVFAEIEKVRSEWCIRIDGEVKAR
DESLVNAKIPTGEIEVFIRDIEVLGKSEELPLMVFGEQEYPEETRLRYRYLDLRREKMQRNMVLRSNMIRSIRNRMWDQG
FNEYQTPIITASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLMMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDME
MSFVTQQDVFDTIAPVIAGVFEEFGQGRKVDAPNEWPQISYKDAALWYGSDKPDLRNPIKMQVVSEHFRGSGFAIFAKLL
EQEGTEIRAIPAPTGGSRKFCDRMNAFAQKEGLPGMGYIFWREGADGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFF
LGGKPKTFEAVAGRARNVIGEELNLTDKDRFAFAWIVDFPIYEKDEETGKIDFEHNPFSMPQGGMDALNGDPLDVRGNQY
DLACNGYELVSGAIRNHKPEIMFKAFEIAGYGKEEVEKRFGGMVNAFQYGAPPHGGCAAGIDRMVMLLADEANIREVIMF
PMNQRAEDLMMAAPSEPMSEQLMELGLRVIPQDD
>Mature_594_residues
MHAFRSHTCAELNKSHVGETVRLSGWVHRVRDHGGLLFIDLRDHYGVTQVMADPDSPVFAEIEKVRSEWCIRIDGEVKAR
DESLVNAKIPTGEIEVFIRDIEVLGKSEELPLMVFGEQEYPEETRLRYRYLDLRREKMQRNMVLRSNMIRSIRNRMWDQG
FNEYQTPIITASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLMMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDME
MSFVTQQDVFDTIAPVIAGVFEEFGQGRKVDAPNEWPQISYKDAALWYGSDKPDLRNPIKMQVVSEHFRGSGFAIFAKLL
EQEGTEIRAIPAPTGGSRKFCDRMNAFAQKEGLPGMGYIFWREGADGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFF
LGGKPKTFEAVAGRARNVIGEELNLTDKDRFAFAWIVDFPIYEKDEETGKIDFEHNPFSMPQGGMDALNGDPLDVRGNQY
DLACNGYELVSGAIRNHKPEIMFKAFEIAGYGKEEVEKRFGGMVNAFQYGAPPHGGCAAGIDRMVMLLADEANIREVIMF
PMNQRAEDLMMAAPSEPMSEQLMELGLRVIPQDD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=605, Percent_Identity=35.3719008264463, Blast_Score=332, Evalue=8e-91,
Organism=Homo sapiens, GI45439306, Length=279, Percent_Identity=27.5985663082437, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI226491502, Length=245, Percent_Identity=24.0816326530612, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI5031815, Length=267, Percent_Identity=26.5917602996255, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194272210, Length=267, Percent_Identity=26.5917602996255, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=40.7407407407407, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=26.9113149847095, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=609, Percent_Identity=32.183908045977, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17551876, Length=285, Percent_Identity=29.8245614035088, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=306, Percent_Identity=23.8562091503268, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=31.2202852614897, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323011, Length=265, Percent_Identity=27.5471698113208, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=307, Percent_Identity=24.1042345276873, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24584738, Length=609, Percent_Identity=32.3481116584565, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17136276, Length=286, Percent_Identity=27.972027972028, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=283, Percent_Identity=28.2685512367491, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640851, Length=283, Percent_Identity=28.2685512367491, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_SILST (Q1GIZ8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612630.1
- ProteinModelPortal:   Q1GIZ8
- SMR:   Q1GIZ8
- STRING:   Q1GIZ8
- GeneID:   4076122
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0635
- NMPDR:   fig|292414.1.peg.2743
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   SSP292414:TM1040_0635-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67165; Mature: 67165

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFRSHTCAELNKSHVGETVRLSGWVHRVRDHGGLLFIDLRDHYGVTQVMADPDSPVFA
CCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHH
EIEKVRSEWCIRIDGEVKARDESLVNAKIPTGEIEVFIRDIEVLGKSEELPLMVFGEQEY
HHHHHHHCCEEEECCCEECCCCCCEECCCCCCCEEEEEEEHHHHCCCCCCCEEEECCCCC
PEETRLRYRYLDLRREKMQRNMVLRSNMIRSIRNRMWDQGFNEYQTPIITASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCHHC
LVPSRLHPGKFYALPQAPQQFKQLMMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDME
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCEEEEECH
MSFVTQQDVFDTIAPVIAGVFEEFGQGRKVDAPNEWPQISYKDAALWYGSDKPDLRNPIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHH
MQVVSEHFRGSGFAIFAKLLEQEGTEIRAIPAPTGGSRKFCDRMNAFAQKEGLPGMGYIF
HHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
WREGADGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLGGKPKTFEAVAGRARNVIG
EECCCCCCHHCCCHHHCCCHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHC
EELNLTDKDRFAFAWIVDFPIYEKDEETGKIDFEHNPFSMPQGGMDALNGDPLDVRGNQY
CCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCEE
DLACNGYELVSGAIRNHKPEIMFKAFEIAGYGKEEVEKRFGGMVNAFQYGAPPHGGCAAG
EEEECCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHH
IDRMVMLLADEANIREVIMFPMNQRAEDLMMAAPSEPMSEQLMELGLRVIPQDD
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCEECCCCC
>Mature Secondary Structure
MHAFRSHTCAELNKSHVGETVRLSGWVHRVRDHGGLLFIDLRDHYGVTQVMADPDSPVFA
CCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHH
EIEKVRSEWCIRIDGEVKARDESLVNAKIPTGEIEVFIRDIEVLGKSEELPLMVFGEQEY
HHHHHHHCCEEEECCCEECCCCCCEECCCCCCCEEEEEEEHHHHCCCCCCCEEEECCCCC
PEETRLRYRYLDLRREKMQRNMVLRSNMIRSIRNRMWDQGFNEYQTPIITASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCCCCCHHC
LVPSRLHPGKFYALPQAPQQFKQLMMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDME
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCEEEEECH
MSFVTQQDVFDTIAPVIAGVFEEFGQGRKVDAPNEWPQISYKDAALWYGSDKPDLRNPIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHH
MQVVSEHFRGSGFAIFAKLLEQEGTEIRAIPAPTGGSRKFCDRMNAFAQKEGLPGMGYIF
HHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
WREGADGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLGGKPKTFEAVAGRARNVIG
EECCCCCCHHCCCHHHCCCHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHC
EELNLTDKDRFAFAWIVDFPIYEKDEETGKIDFEHNPFSMPQGGMDALNGDPLDVRGNQY
CCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCEE
DLACNGYELVSGAIRNHKPEIMFKAFEIAGYGKEEVEKRFGGMVNAFQYGAPPHGGCAAG
EEEECCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHH
IDRMVMLLADEANIREVIMFPMNQRAEDLMMAAPSEPMSEQLMELGLRVIPQDD
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA