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The map label for this gene is mrdA [H]

Identifier: 99080311

GI number: 99080311

Start: 488706

End: 490652

Strand: Reverse

Name: mrdA [H]

Synonym: TM1040_0470

Alternate gene names: 99080311

Gene position: 490652-488706 (Counterclockwise)

Preceding gene: 99080312

Following gene: 99080310

Centisome position: 15.33

GC content: 61.02

Gene sequence:

>1947_bases
GTGAAACGCAATCAGAAAGATCAGGATCACAGCCATCGCAAACTCGCCCGTCGCACCTTGCTGCTCGGGGGGCTTCAGCT
CGGATTCACCGGGGCGCTGGCCATGCGCATGCGGTTTTTGCAGGTGGATCAGGCCGACCAGTTCCGCCTGCTTGCCGAAG
AGAATCGCATCAACATTCGCCTGCTCGCGCCCAGCCGAGGCGAGATCTATGATCGCAACGGCAATATCATCGCTCAGAAC
TCGCCCTCCTACCGGATCGTTCTGGTGCCTGAGGACGCCGGCGATGTGGAAGAGGTGATCGCCAATCTCAACCGGCTGCT
GCCCCTCGACGAAGAGGATCTGGAGCGCGCTCGCAACGAGATGCGCCGCTCGCGTCCTTTCCTGCCCATTACTTTGGCAG
ATCAGATCTCTTGGGATGATATTTCCAAGGTCGCGGTGAATGCCCCGGCCCTGCCCGGCATCACACCCGAGGTCGGCCTC
TCCCGCGTTTATCCACAAGGCCCTGATTTTGCACATGTTGTGGGCTATGTCGGGCCGGTTTCGGACTATGATCTTTCCAA
AATGGAAGACCCGGATCCCGTGCTGCTCACGCCGCGTTTCCAGTTTGGCAAAGTCGGATTTGAGTCCAAGCAAGAGGACT
CGTTGCGTGGCAAAGCCGGGATCAAGCGGGTCGAAGTCAACGCCGCAGGCCGGGTGATGCGAGAGCTTGACCGGCGAGAA
GGTGAGCCGGGCGCGGATATGCAGCTGACGCTGGATGCAGATCTGCAAAGCTATGTGCAGGCGCGCCTTGGCTCCGAGAG
CGCTGCGGCAGTCATTATGGATGTGGAGAACGGCGATCTCAGGGCGATCTGCTCAAGCCCGAGTTTTGATCCCAACCTTT
TTGTGCGCGGGATTTCGGTCGCGGATTATCGCGCGCTCACCGAAGACCCTTACCGCCCGCTGGCCAATAAAACCGTGCAA
GGCACCTACCCGCCCGGCTCCACCTTCAAGATGGTCACTGCCCTTGCAGGACTCGAGGCGGGAGTCATCACACCGGCCGA
GACCACGTGGTGCCCCGGTCACCTCGAGGTTTCGGGGCGGCGGTTCCACTGTTGGAAACGCGCCGGGCATGGCCATGTGA
ACCTCTTGGATTCACTCAAGAGCTCCTGTGACGTCTATTATTACGATCTCGCAATCAAAGTGGGCATGGATCGCATTTCC
GAGATGGCCAACAGGCTTGGGCTTGGTGTACGCCACGATCTGCCGATGTCTGCCGTTGCGGCGGGTCTTGCCCCCAACCG
GGAATGGAAATCACGCACCTACGGTCAGGACTGGCTCGTAGGGGACACGGTGAACGCATCGATTGGACAAGGCTATATGC
TTGCCTCCCCCATGCAGCTTGCGGTGATGACCGCCCGGATTGCATCGGGGCGCGAGATCTCGCCCCGATTGGTGAAATCC
ATCAATGGGATCGAACAGCCCTCCGGCCTGGGGGAGCCACTCGGGCTCAACCAGAACCACCTGAACCATGTCCGCAAATC
CATGTACGCCGTGAGCAATGACCGTCGCGGTACCGGCTACCGCTCGCGGATCATTGCAGAGGACATGCGCATGGCCGGAA
AATCCGGCACCAGCCAGGTGCGCAATATCACCGCCGCCGAACGCGCGTCGGGCGTGATCCGCAACCAGGATCTGCCGTGG
GAGCGGCGCGATCATGCGCTCTATGTCTGCTATGCGCCCTATGACAATCCCAAATACGCTGTCTCGGTCGTGGTGGAACA
CGGCGGGGGCGGCTCCACCGCAGCCGCGCCTATTGCACGGGATGTGCTGCTGCAGGCCCTCTATGGCGGCACGCCCCCGC
TCGAGGCCTACCCGAGAGGCGATCGCAGCAAGATCCAAGCGCAACAGGAACGTCTCCAGCGCGAAATGCATCAGCGTGAG
GAAAGCGGCGGGAGCGACCGGGCATGA

Upstream 100 bases:

>100_bases
TGACGATCGCCGCTTATCCGCTCGTTGTGCTGATAAGCCAGAGCCTGTTCGGAGTACGTCGCCTGTCGGCGGCGGATGCA
GAAACCCTTGGAGCGCGCCT

Downstream 100 bases:

>100_bases
GCTATCTTGAGTATCACGCCAAATCGACCCCGACCGGCCTGCGCAAGATCCTGTTTCTGAACTGGCCCCTGGTGCTGCTG
CTCTGCGCGGCTGCAGGCGT

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MKRNQKDQDHSHRKLARRTLLLGGLQLGFTGALAMRMRFLQVDQADQFRLLAEENRINIRLLAPSRGEIYDRNGNIIAQN
SPSYRIVLVPEDAGDVEEVIANLNRLLPLDEEDLERARNEMRRSRPFLPITLADQISWDDISKVAVNAPALPGITPEVGL
SRVYPQGPDFAHVVGYVGPVSDYDLSKMEDPDPVLLTPRFQFGKVGFESKQEDSLRGKAGIKRVEVNAAGRVMRELDRRE
GEPGADMQLTLDADLQSYVQARLGSESAAAVIMDVENGDLRAICSSPSFDPNLFVRGISVADYRALTEDPYRPLANKTVQ
GTYPPGSTFKMVTALAGLEAGVITPAETTWCPGHLEVSGRRFHCWKRAGHGHVNLLDSLKSSCDVYYYDLAIKVGMDRIS
EMANRLGLGVRHDLPMSAVAAGLAPNREWKSRTYGQDWLVGDTVNASIGQGYMLASPMQLAVMTARIASGREISPRLVKS
INGIEQPSGLGEPLGLNQNHLNHVRKSMYAVSNDRRGTGYRSRIIAEDMRMAGKSGTSQVRNITAAERASGVIRNQDLPW
ERRDHALYVCYAPYDNPKYAVSVVVEHGGGGSTAAAPIARDVLLQALYGGTPPLEAYPRGDRSKIQAQQERLQREMHQRE
ESGGSDRA

Sequences:

>Translated_648_residues
MKRNQKDQDHSHRKLARRTLLLGGLQLGFTGALAMRMRFLQVDQADQFRLLAEENRINIRLLAPSRGEIYDRNGNIIAQN
SPSYRIVLVPEDAGDVEEVIANLNRLLPLDEEDLERARNEMRRSRPFLPITLADQISWDDISKVAVNAPALPGITPEVGL
SRVYPQGPDFAHVVGYVGPVSDYDLSKMEDPDPVLLTPRFQFGKVGFESKQEDSLRGKAGIKRVEVNAAGRVMRELDRRE
GEPGADMQLTLDADLQSYVQARLGSESAAAVIMDVENGDLRAICSSPSFDPNLFVRGISVADYRALTEDPYRPLANKTVQ
GTYPPGSTFKMVTALAGLEAGVITPAETTWCPGHLEVSGRRFHCWKRAGHGHVNLLDSLKSSCDVYYYDLAIKVGMDRIS
EMANRLGLGVRHDLPMSAVAAGLAPNREWKSRTYGQDWLVGDTVNASIGQGYMLASPMQLAVMTARIASGREISPRLVKS
INGIEQPSGLGEPLGLNQNHLNHVRKSMYAVSNDRRGTGYRSRIIAEDMRMAGKSGTSQVRNITAAERASGVIRNQDLPW
ERRDHALYVCYAPYDNPKYAVSVVVEHGGGGSTAAAPIARDVLLQALYGGTPPLEAYPRGDRSKIQAQQERLQREMHQRE
ESGGSDRA
>Mature_648_residues
MKRNQKDQDHSHRKLARRTLLLGGLQLGFTGALAMRMRFLQVDQADQFRLLAEENRINIRLLAPSRGEIYDRNGNIIAQN
SPSYRIVLVPEDAGDVEEVIANLNRLLPLDEEDLERARNEMRRSRPFLPITLADQISWDDISKVAVNAPALPGITPEVGL
SRVYPQGPDFAHVVGYVGPVSDYDLSKMEDPDPVLLTPRFQFGKVGFESKQEDSLRGKAGIKRVEVNAAGRVMRELDRRE
GEPGADMQLTLDADLQSYVQARLGSESAAAVIMDVENGDLRAICSSPSFDPNLFVRGISVADYRALTEDPYRPLANKTVQ
GTYPPGSTFKMVTALAGLEAGVITPAETTWCPGHLEVSGRRFHCWKRAGHGHVNLLDSLKSSCDVYYYDLAIKVGMDRIS
EMANRLGLGVRHDLPMSAVAAGLAPNREWKSRTYGQDWLVGDTVNASIGQGYMLASPMQLAVMTARIASGREISPRLVKS
INGIEQPSGLGEPLGLNQNHLNHVRKSMYAVSNDRRGTGYRSRIIAEDMRMAGKSGTSQVRNITAAERASGVIRNQDLPW
ERRDHALYVCYAPYDNPKYAVSVVVEHGGGGSTAAAPIARDVLLQALYGGTPPLEAYPRGDRSKIQAQQERLQREMHQRE
ESGGSDRA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=622, Percent_Identity=34.887459807074, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786272, Length=488, Percent_Identity=25.2049180327869, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71494; Mature: 71494

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNQKDQDHSHRKLARRTLLLGGLQLGFTGALAMRMRFLQVDQADQFRLLAEENRINIR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
LLAPSRGEIYDRNGNIIAQNSPSYRIVLVPEDAGDVEEVIANLNRLLPLDEEDLERARNE
EECCCCCCEEECCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
MRRSRPFLPITLADQISWDDISKVAVNAPALPGITPEVGLSRVYPQGPDFAHVVGYVGPV
HHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCC
SDYDLSKMEDPDPVLLTPRFQFGKVGFESKQEDSLRGKAGIKRVEVNAAGRVMRELDRRE
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHC
GEPGADMQLTLDADLQSYVQARLGSESAAAVIMDVENGDLRAICSSPSFDPNLFVRGISV
CCCCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECCH
ADYRALTEDPYRPLANKTVQGTYPPGSTFKMVTALAGLEAGVITPAETTWCPGHLEVSGR
HHHHHHCCCCCCHHHCCCEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCC
RFHCWKRAGHGHVNLLDSLKSSCDVYYYDLAIKVGMDRISEMANRLGLGVRHDLPMSAVA
EEEEEHHCCCCCHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHH
AGLAPNREWKSRTYGQDWLVGDTVNASIGQGYMLASPMQLAVMTARIASGREISPRLVKS
HCCCCCCCHHCCCCCCCEEECCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHH
INGIEQPSGLGEPLGLNQNHLNHVRKSMYAVSNDRRGTGYRSRIIAEDMRMAGKSGTSQV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RNITAAERASGVIRNQDLPWERRDHALYVCYAPYDNPKYAVSVVVEHGGGGSTAAAPIAR
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHH
DVLLQALYGGTPPLEAYPRGDRSKIQAQQERLQREMHQREESGGSDRA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRNQKDQDHSHRKLARRTLLLGGLQLGFTGALAMRMRFLQVDQADQFRLLAEENRINIR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
LLAPSRGEIYDRNGNIIAQNSPSYRIVLVPEDAGDVEEVIANLNRLLPLDEEDLERARNE
EECCCCCCEEECCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
MRRSRPFLPITLADQISWDDISKVAVNAPALPGITPEVGLSRVYPQGPDFAHVVGYVGPV
HHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCC
SDYDLSKMEDPDPVLLTPRFQFGKVGFESKQEDSLRGKAGIKRVEVNAAGRVMRELDRRE
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHC
GEPGADMQLTLDADLQSYVQARLGSESAAAVIMDVENGDLRAICSSPSFDPNLFVRGISV
CCCCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECCH
ADYRALTEDPYRPLANKTVQGTYPPGSTFKMVTALAGLEAGVITPAETTWCPGHLEVSGR
HHHHHHCCCCCCHHHCCCEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCC
RFHCWKRAGHGHVNLLDSLKSSCDVYYYDLAIKVGMDRISEMANRLGLGVRHDLPMSAVA
EEEEEHHCCCCCHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHH
AGLAPNREWKSRTYGQDWLVGDTVNASIGQGYMLASPMQLAVMTARIASGREISPRLVKS
HCCCCCCCHHCCCCCCCEEECCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHH
INGIEQPSGLGEPLGLNQNHLNHVRKSMYAVSNDRRGTGYRSRIIAEDMRMAGKSGTSQV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RNITAAERASGVIRNQDLPWERRDHALYVCYAPYDNPKYAVSVVVEHGGGGSTAAAPIAR
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHH
DVLLQALYGGTPPLEAYPRGDRSKIQAQQERLQREMHQREESGGSDRA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]