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The map label for this gene is rpoH2 [H]

Identifier: 99080297

GI number: 99080297

Start: 473334

End: 474212

Strand: Direct

Name: rpoH2 [H]

Synonym: TM1040_0456

Alternate gene names: 99080297

Gene position: 473334-474212 (Clockwise)

Preceding gene: 99080292

Following gene: 99080298

Centisome position: 14.79

GC content: 60.75

Gene sequence:

>879_bases
ATGGCACTGGACGAGACCACCAAGAACCCCCTCCCGAAGCAGGCCATGAAAGCCGAGCTTCTGGATGCAGAGACGGAATT
GGCGTTGGCCTACGCATGGCGCGATGAGAAGGACGTGCAGGCGCTCCATCGTCTGATCACAGCCTACATGCGGCTCGCAG
TGTCGATGGCGTCAAAATTCCGCCGCTATGGCGCCCCGATGAATGATCTCATCCAAGAGGCAGGGCTTGGCCTGATGAAG
GCGGCAGAGAAATTTGATCCCGACCGCGGCGTGCGGTTCTCGACCTACGCGGTCTGGTGGATCAAGGCGTCCATTCAGGA
CTACGTGATGCGCAACTGGTCGATGGTGCGCACTGGCTCGACCTCCTCGCAGAAGTCCCTGTTCTTCAACATGCGCCGTG
TGCAGGCGCAACTCGAGCGCGAGGCAGCCGGCGAAGGCGTGGAACTCGACCGTCACAAGCTGATGCAGATGGTTGCAGAA
GAGATCGGCGTGCCGCTGCGCGATGTCGAGATGATGGACGGCCGCCTCGCGGGTGCTGATTTCTCGCTGAACGCAGTGCA
GTCGGCAGACGAGGACGGGCGCGAGTGGATCGACGCCTTGGAAGATGACAGCGAACAGGCTGCGGACCGCGTGGAGGCCG
ATCATGATCGTCGCCAGCTGCGCGAGTGGCTGCTCTCGGCGCTGAATGGTCTCAATGAGCGCGAGCGCTTTATCGTGCGC
GAGCGCAAACTGAGAGAAGAGGCGCGCACTCTGGAGAGCCTCGGCAATGAATTGGGACTGTCAAAAGAGCGCGTGCGTCA
GCTGGAAGCCGCCGCCTTCTCAAAGATGAGAAAGAGCCTTGAAGGTCAGTCCCGTGAGGTGCAGAACTTCCTTGCATGA

Upstream 100 bases:

>100_bases
AGGATGCATAGAGCATCTTTTGAATATCCGACAGATTATTTTTGAACCAAAGCCGGCCCTCGCACGTTGTCAACGTAGTT
CTTAGAACAGGGAGTTTAAA

Downstream 100 bases:

>100_bases
GGAATATGCATTTTAGGCGCGGACCTGGAGGTCGGCAATTTCATCTGGCAGCAGTTCTTGCACTTTTGCCTTTTATTACA
AACGCCCAAGCGCTTGCGGA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MALDETTKNPLPKQAMKAELLDAETELALAYAWRDEKDVQALHRLITAYMRLAVSMASKFRRYGAPMNDLIQEAGLGLMK
AAEKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQAQLEREAAGEGVELDRHKLMQMVAE
EIGVPLRDVEMMDGRLAGADFSLNAVQSADEDGREWIDALEDDSEQAADRVEADHDRRQLREWLLSALNGLNERERFIVR
ERKLREEARTLESLGNELGLSKERVRQLEAAAFSKMRKSLEGQSREVQNFLA

Sequences:

>Translated_292_residues
MALDETTKNPLPKQAMKAELLDAETELALAYAWRDEKDVQALHRLITAYMRLAVSMASKFRRYGAPMNDLIQEAGLGLMK
AAEKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQAQLEREAAGEGVELDRHKLMQMVAE
EIGVPLRDVEMMDGRLAGADFSLNAVQSADEDGREWIDALEDDSEQAADRVEADHDRRQLREWLLSALNGLNERERFIVR
ERKLREEARTLESLGNELGLSKERVRQLEAAAFSKMRKSLEGQSREVQNFLA
>Mature_291_residues
ALDETTKNPLPKQAMKAELLDAETELALAYAWRDEKDVQALHRLITAYMRLAVSMASKFRRYGAPMNDLIQEAGLGLMKA
AEKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQAQLEREAAGEGVELDRHKLMQMVAEE
IGVPLRDVEMMDGRLAGADFSLNAVQSADEDGREWIDALEDDSEQAADRVEADHDRRQLREWLLSALNGLNERERFIVRE
RKLREEARTLESLGNELGLSKERVRQLEAAAFSKMRKSLEGQSREVQNFLA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=266, Percent_Identity=38.7218045112782, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789098, Length=278, Percent_Identity=32.7338129496403, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=29.4820717131474, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33447; Mature: 33316

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDETTKNPLPKQAMKAELLDAETELALAYAWRDEKDVQALHRLITAYMRLAVSMASKF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRYGAPMNDLIQEAGLGLMKAAEKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGS
HHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHEECCC
TSSQKSLFFNMRRVQAQLEREAAGEGVELDRHKLMQMVAEEIGVPLRDVEMMDGRLAGAD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCEECCCC
FSLNAVQSADEDGREWIDALEDDSEQAADRVEADHDRRQLREWLLSALNGLNERERFIVR
CCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHH
ERKLREEARTLESLGNELGLSKERVRQLEAAAFSKMRKSLEGQSREVQNFLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
ALDETTKNPLPKQAMKAELLDAETELALAYAWRDEKDVQALHRLITAYMRLAVSMASKF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRYGAPMNDLIQEAGLGLMKAAEKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGS
HHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHEECCC
TSSQKSLFFNMRRVQAQLEREAAGEGVELDRHKLMQMVAEEIGVPLRDVEMMDGRLAGAD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCEECCCC
FSLNAVQSADEDGREWIDALEDDSEQAADRVEADHDRRQLREWLLSALNGLNERERFIVR
CCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHH
ERKLREEARTLESLGNELGLSKERVRQLEAAAFSKMRKSLEGQSREVQNFLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]