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The map label for this gene is est [H]

Identifier: 99080292

GI number: 99080292

Start: 468806

End: 469702

Strand: Direct

Name: est [H]

Synonym: TM1040_0451

Alternate gene names: 99080292

Gene position: 468806-469702 (Clockwise)

Preceding gene: 99080291

Following gene: 99080297

Centisome position: 14.65

GC content: 64.55

Gene sequence:

>897_bases
GTGAGTCTTGCGCTTGATTTGCTATTGGTTGGGCTGCGCCATGTGGTGCGGCCCCGCCTGCGCCGTGCGCATGGCCCGCT
AGAGGCGCGCCGAGCCTTTGACCGCGCGGCAGCGGTGACATTCCGGGCGCCGCCCTTCCTTTGTCGCATCAAGGACCGGG
CAGGGCTCGACCGGATCCACTGTGGTCCGCGCCTGCATGGCAAAATTATTCTTTATTTTCACGGCGGTGGCTATGTGGCC
GGTGGCCCGCATACGCATGCAGCCATGCTCGGGCAGCTGTCAGGGCTTTCACAGCTTGAGGTCTGCGCGCCGGCGTATCC
GCTGGCCCCCGAACATCCTTTTCCGGCGGCGTTCGAGGGCGCCGTGGCATCTTGGGAGCATCTGATGGCTGCGGGTTACG
CGCCGTCGGATGTGGTGTTGGGCGGCGATTCTGCCGGTGGCGGCCTCGCGCTCGCGCTGCTGTCCTATCTATGCCACGCG
GCAACACCGCCTGCGGCCGCGTTTGCGATGTCGCCCTGGTGCGATCTGAGCCTGAGTGGCGACAGCCTGCAGGGCAATGC
AGAGCGAGATCCGCTCCTGCCCGCAGAGCGTATCCGGGAACTTGTGGGCTATATCCTGCCCGAAGGCGCCGATGACACGG
ACCCGCGGGTGTCGCCACTTTTTGCAGAGTTCCCCACCTGCCCGCCGGTCTTGCTGCAGTATAGCGAGACGGAAATCCTG
TTTGATGATTGCCGCCGGATGGCAGAGCGCCTCAGGGGTTTTGACGCTGACGTTCTGGAGAGGCGGCATCCCCGCGCACC
ACATGTCTGGCATCTGTTTCAGGGCTGGGTCCCCGAGGCCCGTAAGTCGCTGAGGGAGATTGCCGATTTTGCACGCGCGG
CGTTTACTGCGCGCTAA

Upstream 100 bases:

>100_bases
AGATCAACGTGACCTCTCCGACCGGAATTCAGGAGCTTGAGCGCTTTAACGGCGTCAATATCGCGGAAAAAGTCTGGGAG
GCGATTGAGGCGCGCGTTGC

Downstream 100 bases:

>100_bases
CTGGGCAGGCGGTAGGACAGTGCCTCGGCTACATGATCGCGGGCGATCTGCGCTTCGGCATCCAGATCCGCAATGGTGCG
GGCAACGCGCAAGACCCGGT

Product: Alpha/beta hydrolase fold-3

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLALDLLLVGLRHVVRPRLRRAHGPLEARRAFDRAAAVTFRAPPFLCRIKDRAGLDRIHCGPRLHGKIILYFHGGGYVA
GGPHTHAAMLGQLSGLSQLEVCAPAYPLAPEHPFPAAFEGAVASWEHLMAAGYAPSDVVLGGDSAGGGLALALLSYLCHA
ATPPAAAFAMSPWCDLSLSGDSLQGNAERDPLLPAERIRELVGYILPEGADDTDPRVSPLFAEFPTCPPVLLQYSETEIL
FDDCRRMAERLRGFDADVLERRHPRAPHVWHLFQGWVPEARKSLREIADFARAAFTAR

Sequences:

>Translated_298_residues
MSLALDLLLVGLRHVVRPRLRRAHGPLEARRAFDRAAAVTFRAPPFLCRIKDRAGLDRIHCGPRLHGKIILYFHGGGYVA
GGPHTHAAMLGQLSGLSQLEVCAPAYPLAPEHPFPAAFEGAVASWEHLMAAGYAPSDVVLGGDSAGGGLALALLSYLCHA
ATPPAAAFAMSPWCDLSLSGDSLQGNAERDPLLPAERIRELVGYILPEGADDTDPRVSPLFAEFPTCPPVLLQYSETEIL
FDDCRRMAERLRGFDADVLERRHPRAPHVWHLFQGWVPEARKSLREIADFARAAFTAR
>Mature_297_residues
SLALDLLLVGLRHVVRPRLRRAHGPLEARRAFDRAAAVTFRAPPFLCRIKDRAGLDRIHCGPRLHGKIILYFHGGGYVAG
GPHTHAAMLGQLSGLSQLEVCAPAYPLAPEHPFPAAFEGAVASWEHLMAAGYAPSDVVLGGDSAGGGLALALLSYLCHAA
TPPAAAFAMSPWCDLSLSGDSLQGNAERDPLLPAERIRELVGYILPEGADDTDPRVSPLFAEFPTCPPVLLQYSETEILF
DDCRRMAERLRGFDADVLERRHPRAPHVWHLFQGWVPEARKSLREIADFARAAFTAR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=296, Percent_Identity=26.3513513513513, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32473; Mature: 32341

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLALDLLLVGLRHVVRPRLRRAHGPLEARRAFDRAAAVTFRAPPFLCRIKDRAGLDRIH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEECCCCCCCHHC
CGPRLHGKIILYFHGGGYVAGGPHTHAAMLGQLSGLSQLEVCAPAYPLAPEHPFPAAFEG
CCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHH
AVASWEHLMAAGYAPSDVVLGGDSAGGGLALALLSYLCHAATPPAAAFAMSPWCDLSLSG
HHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEECC
DSLQGNAERDPLLPAERIRELVGYILPEGADDTDPRVSPLFAEFPTCPPVLLQYSETEIL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHEECCCHHHH
FDDCRRMAERLRGFDADVLERRHPRAPHVWHLFQGWVPEARKSLREIADFARAAFTAR
HHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLALDLLLVGLRHVVRPRLRRAHGPLEARRAFDRAAAVTFRAPPFLCRIKDRAGLDRIH
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEECCCCCCCHHC
CGPRLHGKIILYFHGGGYVAGGPHTHAAMLGQLSGLSQLEVCAPAYPLAPEHPFPAAFEG
CCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHH
AVASWEHLMAAGYAPSDVVLGGDSAGGGLALALLSYLCHAATPPAAAFAMSPWCDLSLSG
HHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEECC
DSLQGNAERDPLLPAERIRELVGYILPEGADDTDPRVSPLFAEFPTCPPVLLQYSETEIL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHEECCCHHHH
FDDCRRMAERLRGFDADVLERRHPRAPHVWHLFQGWVPEARKSLREIADFARAAFTAR
HHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]