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The map label for this gene is gshB [H]

Identifier: 99080291

GI number: 99080291

Start: 467874

End: 468809

Strand: Direct

Name: gshB [H]

Synonym: TM1040_0450

Alternate gene names: 99080291

Gene position: 467874-468809 (Clockwise)

Preceding gene: 99080290

Following gene: 99080292

Centisome position: 14.62

GC content: 58.87

Gene sequence:

>936_bases
ATGAAAATCGCATTTCAGATGGACCCGATCATGGGCGTCGATGTGAACGCCGACAGCAGCTTTCGCCTTGCGGAAGAGGC
GCAGAAACGCGGTCACGAGCTGTTCTATTACAGCCCCGATCAGCTTGCCTACCAGGAAGGGCGCATCACGGCGCGCGGGC
ATGACATGGTGGTGCAACGTGTCGAGGGCACGCCGGCGACGCTCGGAGAGCTGCGGGAGGTAGACCTGGCAGATTTCGAC
GTGATCTGGCTGCGCCAGGATCCGCCGTTTGACATGCATTACATCACCTCGACGCATCTTTTGGATCGTCTGAAGGGCCA
GGCGCTTGTGGTCAATGATCCCTTCTGGGTGCGCAACTACCCTGAGAAACTGCTGGTTCTGAACTTCCCTGATCTCACGC
CGCCGACGACGATTGCGCGCGATCTTGAGACCATCAAGGCCTTCAAGGCCAAACATGGCGATGTGATCCTGAAGCCGCTC
TACGGTAATGGTGGTGCCGGGGTGTTCCGTCTGGATGCCAATGACCGCAACTTGTCGTCGCTGCATGAGCTCTTTACCGG
CTTCAGCCGCGAGCCTCTCATTGTGCAGAAATTCCTCCCGGATGTTTCAAATGGTGACAAGCGCGTCATCCTCGTCGACG
GAGAGCCTGTGGGCGCCATCAACCGCGTGCCCGCGGCGGGCGAGACACGGTCCAACATGCATGTGGGCGGGCGGCCTGAA
AAAATCGGGCTCACGGAGCGCGATCTGGAGATCTGTGCCGCGATTGGACCGCTCCTCAAGGAGAAGGGGCAGGTGTTTGT
CGGCATCGACGTGATCGGCGATTATCTGACAGAGATCAACGTGACCTCTCCGACCGGAATTCAGGAGCTTGAGCGCTTTA
ACGGCGTCAATATCGCGGAAAAAGTCTGGGAGGCGATTGAGGCGCGCGTTGCGTGA

Upstream 100 bases:

>100_bases
CGAGGTGGAGATTATCGAAAATGCCTATGGCCACGGCTGAGCCATTGAACATGGCCAGCGCCTACGCCATGTATCTGTTG
TGAAACAAGGGACACATATT

Downstream 100 bases:

>100_bases
GTCTTGCGCTTGATTTGCTATTGGTTGGGCTGCGCCATGTGGTGCGGCCCCGCCTGCGCCGTGCGCATGGCCCGCTAGAG
GCGCGCCGAGCCTTTGACCG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKIAFQMDPIMGVDVNADSSFRLAEEAQKRGHELFYYSPDQLAYQEGRITARGHDMVVQRVEGTPATLGELREVDLADFD
VIWLRQDPPFDMHYITSTHLLDRLKGQALVVNDPFWVRNYPEKLLVLNFPDLTPPTTIARDLETIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLSSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KIGLTERDLEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFNGVNIAEKVWEAIEARVA

Sequences:

>Translated_311_residues
MKIAFQMDPIMGVDVNADSSFRLAEEAQKRGHELFYYSPDQLAYQEGRITARGHDMVVQRVEGTPATLGELREVDLADFD
VIWLRQDPPFDMHYITSTHLLDRLKGQALVVNDPFWVRNYPEKLLVLNFPDLTPPTTIARDLETIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLSSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KIGLTERDLEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFNGVNIAEKVWEAIEARVA
>Mature_311_residues
MKIAFQMDPIMGVDVNADSSFRLAEEAQKRGHELFYYSPDQLAYQEGRITARGHDMVVQRVEGTPATLGELREVDLADFD
VIWLRQDPPFDMHYITSTHLLDRLKGQALVVNDPFWVRNYPEKLLVLNFPDLTPPTTIARDLETIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLSSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KIGLTERDLEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFNGVNIAEKVWEAIEARVA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=45.6869009584665, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34631; Mature: 34631

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFQMDPIMGVDVNADSSFRLAEEAQKRGHELFYYSPDQLAYQEGRITARGHDMVVQR
CEEEEEECCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHCCEEEECCHHEEEEE
VEGTPATLGELREVDLADFDVIWLRQDPPFDMHYITSTHLLDRLKGQALVVNDPFWVRNY
CCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCHHCCCC
PEKLLVLNFPDLTPPTTIARDLETIKAFKAKHGDVILKPLYGNGGAGVFRLDANDRNLSS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCHHH
LHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
HHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KIGLTERDLEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFNGVNIAE
CCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHEECCCCCCCHHHHHHHCCCCHHH
KVWEAIEARVA
HHHHHHHHCCC
>Mature Secondary Structure
MKIAFQMDPIMGVDVNADSSFRLAEEAQKRGHELFYYSPDQLAYQEGRITARGHDMVVQR
CEEEEEECCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHCCEEEECCHHEEEEE
VEGTPATLGELREVDLADFDVIWLRQDPPFDMHYITSTHLLDRLKGQALVVNDPFWVRNY
CCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCHHCCCC
PEKLLVLNFPDLTPPTTIARDLETIKAFKAKHGDVILKPLYGNGGAGVFRLDANDRNLSS
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCHHH
LHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
HHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KIGLTERDLEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFNGVNIAE
CCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHEECCCCCCCHHHHHHHCCCCHHH
KVWEAIEARVA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]