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The map label for this gene is paaI [H]

Identifier: 99080280

GI number: 99080280

Start: 456106

End: 456537

Strand: Direct

Name: paaI [H]

Synonym: TM1040_0439

Alternate gene names: 99080280

Gene position: 456106-456537 (Clockwise)

Preceding gene: 99080279

Following gene: 99080281

Centisome position: 14.25

GC content: 61.57

Gene sequence:

>432_bases
ATGTCATTGACCGCACAACAACGGGCAGAGAAATCCGCACAGGCCATGTGGGCCGACGATCAGGCGTCCAAATGGGCCGG
CATGGGCATCGAACGTGTCGACGAGGGCAGTGCCGCGCTGGCCCTGACCGTCGAAGCGCATCACTGCAACGGCCATGGGA
TCTGCCATGGCGGCGTGATCTTCATGCTCGCCGACAGCGCCTTTGCCTTTGCCTGTAACAGCCGCAACCAGGCGACGGTG
GCGCAGCACAATATGATCAGCTTTATCGCGCCAGGTCGCAAAGGCGACAGGCTACTGGCCGAGGCGCGTGAGGTTTCTCT
GACCGGGCGCAGCGGCATCTATGACGTGAGCGTCAAGAATCAGGACGGGCAGCTGATTGCAGAGATGCGTGGCTGCTCGC
GCGCGATCAAGGGACACCTCTTCGAGGAATGA

Upstream 100 bases:

>100_bases
GGCGGATGCGATGAAGACCTGTGGCGAGAGCGCGGATTACGCCGAAGGTGTGCGCGCCTTTCTGGAAAAACGAACGCCCG
TGTTTCGGGGGCAATAATCC

Downstream 100 bases:

>100_bases
CCCGAGGCACCCGGACCACGGGTGCAGACAGACACCAAGGCGATGGAGGACGCCCATGCTTGATTTGACACCAAACCGGG
ACGAATTGGATCCGATCGAG

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSLTAQQRAEKSAQAMWADDQASKWAGMGIERVDEGSAALALTVEAHHCNGHGICHGGVIFMLADSAFAFACNSRNQATV
AQHNMISFIAPGRKGDRLLAEAREVSLTGRSGIYDVSVKNQDGQLIAEMRGCSRAIKGHLFEE

Sequences:

>Translated_143_residues
MSLTAQQRAEKSAQAMWADDQASKWAGMGIERVDEGSAALALTVEAHHCNGHGICHGGVIFMLADSAFAFACNSRNQATV
AQHNMISFIAPGRKGDRLLAEAREVSLTGRSGIYDVSVKNQDGQLIAEMRGCSRAIKGHLFEE
>Mature_142_residues
SLTAQQRAEKSAQAMWADDQASKWAGMGIERVDEGSAALALTVEAHHCNGHGICHGGVIFMLADSAFAFACNSRNQATVA
QHNMISFIAPGRKGDRLLAEAREVSLTGRSGIYDVSVKNQDGQLIAEMRGCSRAIKGHLFEE

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=136, Percent_Identity=41.9117647058824, Blast_Score=105, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15362; Mature: 15230

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTAQQRAEKSAQAMWADDQASKWAGMGIERVDEGSAALALTVEAHHCNGHGICHGGVI
CCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEECCCEE
FMLADSAFAFACNSRNQATVAQHNMISFIAPGRKGDRLLAEAREVSLTGRSGIYDVSVKN
EEEECCCEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHEEECCCCCEEEEEEEC
QDGQLIAEMRGCSRAIKGHLFEE
CCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLTAQQRAEKSAQAMWADDQASKWAGMGIERVDEGSAALALTVEAHHCNGHGICHGGVI
CCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEECCCEE
FMLADSAFAFACNSRNQATVAQHNMISFIAPGRKGDRLLAEAREVSLTGRSGIYDVSVKN
EEEECCCEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHEEECCCCCEEEEEEEC
QDGQLIAEMRGCSRAIKGHLFEE
CCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]