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The map label for this gene is paaK [H]

Identifier: 99080281

GI number: 99080281

Start: 456593

End: 457903

Strand: Direct

Name: paaK [H]

Synonym: TM1040_0440

Alternate gene names: 99080281

Gene position: 456593-457903 (Clockwise)

Preceding gene: 99080280

Following gene: 99080282

Centisome position: 14.26

GC content: 61.56

Gene sequence:

>1311_bases
ATGCTTGATTTGACACCAAACCGGGACGAATTGGATCCGATCGAGATCGCGTCCATCGACGAGATCCGCAGCCTGCAATT
GGAGCGCCTGAAATGGTCGCTGCGCCACGCCTATGACAATGTGGCGATGTATAAGGCGCGGTTTGATGCAGCGGGCGTCC
ACCCCGACGACCTGCAGGAGCTGTCGGATCTCGCAAAGTTCCCCTTCACCACCAAAGGTGATCTGCGCGATGCCTATCCT
TTTGGGATGTTTGCGGTCCCGCGCGAACAGATCCTCCGGCTGCATGCGTCTTCTGGCACCACGGGCAAGCCGACCGTGGT
GGGGTACACGCGAAAGGACATCGACACTTGGGCGGACCTCTTGGCGCGGTCCTTGCGCGCCTCCGGCCTGCGGCGCGGCG
ATATGGTGCATAACGCCTATGGCTACGGGCTGTTCACTGGCGGGCTTGGCGCACATTACGGCATTGAGCGCCTCGGCGCG
ACGGTGGTGCCGATGTCGGGCGGCCAGACCGAAAAGCAAGTGACGCTGATTGAGGATTTCCGCCCCGACGGCATCATGGT
GACGCCGTCCTACATGCTGAACATCCTCGAGCAATATCACCGCATGGGGATCGATCCGCGTAGCTCTTCGCTGAAGGTTG
GCGTCTTTGGCGCGGAACCCTGGACCGATGCGATGCGCCGCGAGGTCGAGGCGGCCTTTGATATGCATGCGGTGGATATC
TATGGTCTCTCGGAAATCATGGGGCCGGGTGTCGCCAATGAATGTGTGGAGACGAAGGACGGGCCGGTCATCTGGGAGGA
TCACTTCCTGCCGGAAATCATCGACCCCGAAACAGGCGCGGTGCTGCCCGATGGCGAGTTGGGCGAACTGGTCTTCACAA
CACTCACCAAGGAAGGCCTGCCGATGGTGCGCTACCGCACCCGCGACCTGACACGCCTGCTCCCGGGCACGGCGCGTTCA
ATGCGGCGGATGGCCAAGATCACCGGCCGGTCGGATGACATGATCATCCTGCGGGGCGTCAATGTGTTTCCCAGCCAGAT
CGAAGAGCAGATGCTCACGGTAGAGGCGCTCGCGCCCTACTATCAGATCGAGCTTTACAGCAAGGGCCGCATGGACGCGA
TGCGCATCCATGTCGAGGTCTTGCCAGCGGCCACCGCACCCGAACAACGCGAGGCCTGCACCAAGCAGCTGGCCAAGCGG
ATCAAGGATATCGTTGGCATCTCGGCAGAGATTTCGGTAGGCGAGCCCGGCAGTGTCGAACGCTCGCAGGGCAAGGCGCG
CCGCGTCGTCGACAATCGCAACAAAGGATAA

Upstream 100 bases:

>100_bases
CGTGGCTGCTCGCGCGCGATCAAGGGACACCTCTTCGAGGAATGACCCGAGGCACCCGGACCACGGGTGCAGACAGACAC
CAAGGCGATGGAGGACGCCC

Downstream 100 bases:

>100_bases
GAACTCTTGGCCCGGACGATTGCCAAAGACCACGATCAAAAGCGCGCGCAGATCCTCGCGGCATCGGCGCGCCTTTTTGC
CGAGGAAGGCTATGACCGGG

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLDLTPNRDELDPIEIASIDEIRSLQLERLKWSLRHAYDNVAMYKARFDAAGVHPDDLQELSDLAKFPFTTKGDLRDAYP
FGMFAVPREQILRLHASSGTTGKPTVVGYTRKDIDTWADLLARSLRASGLRRGDMVHNAYGYGLFTGGLGAHYGIERLGA
TVVPMSGGQTEKQVTLIEDFRPDGIMVTPSYMLNILEQYHRMGIDPRSSSLKVGVFGAEPWTDAMRREVEAAFDMHAVDI
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPETGAVLPDGELGELVFTTLTKEGLPMVRYRTRDLTRLLPGTARS
MRRMAKITGRSDDMIILRGVNVFPSQIEEQMLTVEALAPYYQIELYSKGRMDAMRIHVEVLPAATAPEQREACTKQLAKR
IKDIVGISAEISVGEPGSVERSQGKARRVVDNRNKG

Sequences:

>Translated_436_residues
MLDLTPNRDELDPIEIASIDEIRSLQLERLKWSLRHAYDNVAMYKARFDAAGVHPDDLQELSDLAKFPFTTKGDLRDAYP
FGMFAVPREQILRLHASSGTTGKPTVVGYTRKDIDTWADLLARSLRASGLRRGDMVHNAYGYGLFTGGLGAHYGIERLGA
TVVPMSGGQTEKQVTLIEDFRPDGIMVTPSYMLNILEQYHRMGIDPRSSSLKVGVFGAEPWTDAMRREVEAAFDMHAVDI
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPETGAVLPDGELGELVFTTLTKEGLPMVRYRTRDLTRLLPGTARS
MRRMAKITGRSDDMIILRGVNVFPSQIEEQMLTVEALAPYYQIELYSKGRMDAMRIHVEVLPAATAPEQREACTKQLAKR
IKDIVGISAEISVGEPGSVERSQGKARRVVDNRNKG
>Mature_436_residues
MLDLTPNRDELDPIEIASIDEIRSLQLERLKWSLRHAYDNVAMYKARFDAAGVHPDDLQELSDLAKFPFTTKGDLRDAYP
FGMFAVPREQILRLHASSGTTGKPTVVGYTRKDIDTWADLLARSLRASGLRRGDMVHNAYGYGLFTGGLGAHYGIERLGA
TVVPMSGGQTEKQVTLIEDFRPDGIMVTPSYMLNILEQYHRMGIDPRSSSLKVGVFGAEPWTDAMRREVEAAFDMHAVDI
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPETGAVLPDGELGELVFTTLTKEGLPMVRYRTRDLTRLLPGTARS
MRRMAKITGRSDDMIILRGVNVFPSQIEEQMLTVEALAPYYQIELYSKGRMDAMRIHVEVLPAATAPEQREACTKQLAKR
IKDIVGISAEISVGEPGSVERSQGKARRVVDNRNKG

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=428, Percent_Identity=64.9532710280374, Blast_Score=587, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48541; Mature: 48541

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLTPNRDELDPIEIASIDEIRSLQLERLKWSLRHAYDNVAMYKARFDAAGVHPDDLQE
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LSDLAKFPFTTKGDLRDAYPFGMFAVPREQILRLHASSGTTGKPTVVGYTRKDIDTWADL
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHEEECCCCCCCCCEEEEEHHHHHHHHHHH
LARSLRASGLRRGDMVHNAYGYGLFTGGLGAHYGIERLGATVVPMSGGQTEKQVTLIEDF
HHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHCCEEEECCCCCCCCEEEEEHHC
RPDGIMVTPSYMLNILEQYHRMGIDPRSSSLKVGVFGAEPWTDAMRREVEAAFDMHAVDI
CCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEH
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPETGAVLPDGELGELVFTTLTKEGL
HCHHHHHCCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PMVRYRTRDLTRLLPGTARSMRRMAKITGRSDDMIILRGVNVFPSQIEEQMLTVEALAPY
CEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCE
YQIELYSKGRMDAMRIHVEVLPAATAPEQREACTKQLAKRIKDIVGISAEISVGEPGSVE
EEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
RSQGKARRVVDNRNKG
HHCCHHHHHHCCCCCC
>Mature Secondary Structure
MLDLTPNRDELDPIEIASIDEIRSLQLERLKWSLRHAYDNVAMYKARFDAAGVHPDDLQE
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LSDLAKFPFTTKGDLRDAYPFGMFAVPREQILRLHASSGTTGKPTVVGYTRKDIDTWADL
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHEEECCCCCCCCCEEEEEHHHHHHHHHHH
LARSLRASGLRRGDMVHNAYGYGLFTGGLGAHYGIERLGATVVPMSGGQTEKQVTLIEDF
HHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHCCEEEECCCCCCCCEEEEEHHC
RPDGIMVTPSYMLNILEQYHRMGIDPRSSSLKVGVFGAEPWTDAMRREVEAAFDMHAVDI
CCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEH
YGLSEIMGPGVANECVETKDGPVIWEDHFLPEIIDPETGAVLPDGELGELVFTTLTKEGL
HCHHHHHCCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PMVRYRTRDLTRLLPGTARSMRRMAKITGRSDDMIILRGVNVFPSQIEEQMLTVEALAPY
CEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCE
YQIELYSKGRMDAMRIHVEVLPAATAPEQREACTKQLAKRIKDIVGISAEISVGEPGSVE
EEEEEECCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
RSQGKARRVVDNRNKG
HHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]