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The map label for this gene is mtlK [H]

Identifier: 99080267

GI number: 99080267

Start: 437908

End: 439386

Strand: Reverse

Name: mtlK [H]

Synonym: TM1040_0426

Alternate gene names: 99080267

Gene position: 439386-437908 (Counterclockwise)

Preceding gene: 99080268

Following gene: 99080262

Centisome position: 13.73

GC content: 62.61

Gene sequence:

>1479_bases
ATGACCTCGCTGCGCCTTTCAAACGACACTCTCTCGGATTTGCCCAAACATATCACGCGCCCGACCTATGATCGCGCGGC
GCTGACGGCGGGCATCCTTCACATCGGGCTTGGCAATTTTCACCGTGCCCATCAAAGCTGGTACTTGCACAGACTGATGC
AGCAGGGCGAGGCCCAGGATTGGGCCATCATCGGCGCTGGCGTGCGCGACTATGACGCCGCGATGCGCGCGCGCCTCAAG
GCGCAGGACTGTCTCACCACCCTGATCGCTCTCTCCCCTGACACCACGTCCGCCGAGGTGGTTGGCTCTATGATCGACTA
CCTGCCGATCGAGGCTGACAATGGCGCGCTGATTGCGGCCATGGCCCGCCCCGAGATCCGCATTGTCTCCATGACGGTGA
CCGAAGGCGGGTATTACCGGGATCCGGTCTCGGGCGCGTTTGATGACACACATCCGGATATCCTGCACGATATTGCCAAT
CCAAGCACCCCGCGCACCGTGTTCGGCGCGATCATTGCGGCCCTTGCGCTGCGCCGCACCGCCGGTGTTGGGCCGTTCTC
TGTACAAAGTTGCGACAACCTGCAGGGCAATGGCGATGTCACCCGCCAGACCGTGCTTGGCCTGGCCCGTCGCAGCGATC
CAGAGCTCGCAAGCTGGATTGCGGCGCAATGCACGTTTCCCAATTCCATGGTCGATTGCATTGTCCCCGCAACCGGCCCG
GCAGAGATAGCGCTTGCTGCGGAAATGGGCGTCGCCGATCAGGCCCCCGTCACCCATGAGCCCTATCGCCAATGGGTGAT
CGAGGACAATTTCTGCGCCGGGCGTCCGGATTGGGACAAGGTCGGGGCGACCTTCACCAATCAGGTCCACGCCTATGAGA
CGATGAAGATCCGTATCCTGAACGCCGGGCACCAGGTCATCGCCAATGCGGGCGAGCTATTGTCCTGTGAGACGATCGCG
GACTGCATGCAGGATCCCGACATTCGGTCCATGTTCGAGAAGGTGGAGACCCAAGAGATCGCGCCCTATGTATCGCCGGT
GCCTGGCATCACAGCGGACGCTTATGTGGCGTTGATTGCGGACCGGTTCTCGAACCCAGCCATCCACGACACCACCCGGC
GGGTGGCGTTCGACGGCTCTTCACGGCACGCGGGCTTTATCCTGCCGATCCTGCGTGACGCGCTGGCTGCGCGGGGTCCG
GTCGATGGTCTCGCTCTGGTCGAGGCCCTCTGGGCGCGGATGTGCGCTGGGCTGCGCGAAGACGGAACCGAGATCGAAGC
CAATGACCCCAACTGGCCGCAGTTGCAACAAGCGGCGCAGGCCGCAAGGACCGCCCCCGAGGTCTGGCTGGCGCAGTCCG
AATATTACGGAGATCTCGCTCAGGAGACGCACTTTGCGGACCGGTTCTGCCACTGGCATGCACTGATCTGGAGCAAAGGA
ACACGCGAGGCGCTCCGTTCCTACACGACTCAGATTTGA

Upstream 100 bases:

>100_bases
CAGATGATCTGACCGGTATGGCGATCTTTCTCGCCAGCGCTGAGGCCGACTATGTGGTGTCGCAGACCTATAACGTCGAC
GGCGGAAACTGGATGAGCTG

Downstream 100 bases:

>100_bases
CCTCGCGCGCCCCGGGCTGGGGCGCGCCACCTAGCAGGTGGGCGTTTCAGTCAGATCGCTCAATCCCAGATCACCAAGGG
ATTTGAGCGCGGATTTGTTT

Product: mannitol dehydrogenase-like

Products: NA

Alternate protein names: M2DH; MDH [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTSLRLSNDTLSDLPKHITRPTYDRAALTAGILHIGLGNFHRAHQSWYLHRLMQQGEAQDWAIIGAGVRDYDAAMRARLK
AQDCLTTLIALSPDTTSAEVVGSMIDYLPIEADNGALIAAMARPEIRIVSMTVTEGGYYRDPVSGAFDDTHPDILHDIAN
PSTPRTVFGAIIAALALRRTAGVGPFSVQSCDNLQGNGDVTRQTVLGLARRSDPELASWIAAQCTFPNSMVDCIVPATGP
AEIALAAEMGVADQAPVTHEPYRQWVIEDNFCAGRPDWDKVGATFTNQVHAYETMKIRILNAGHQVIANAGELLSCETIA
DCMQDPDIRSMFEKVETQEIAPYVSPVPGITADAYVALIADRFSNPAIHDTTRRVAFDGSSRHAGFILPILRDALAARGP
VDGLALVEALWARMCAGLREDGTEIEANDPNWPQLQQAAQAARTAPEVWLAQSEYYGDLAQETHFADRFCHWHALIWSKG
TREALRSYTTQI

Sequences:

>Translated_492_residues
MTSLRLSNDTLSDLPKHITRPTYDRAALTAGILHIGLGNFHRAHQSWYLHRLMQQGEAQDWAIIGAGVRDYDAAMRARLK
AQDCLTTLIALSPDTTSAEVVGSMIDYLPIEADNGALIAAMARPEIRIVSMTVTEGGYYRDPVSGAFDDTHPDILHDIAN
PSTPRTVFGAIIAALALRRTAGVGPFSVQSCDNLQGNGDVTRQTVLGLARRSDPELASWIAAQCTFPNSMVDCIVPATGP
AEIALAAEMGVADQAPVTHEPYRQWVIEDNFCAGRPDWDKVGATFTNQVHAYETMKIRILNAGHQVIANAGELLSCETIA
DCMQDPDIRSMFEKVETQEIAPYVSPVPGITADAYVALIADRFSNPAIHDTTRRVAFDGSSRHAGFILPILRDALAARGP
VDGLALVEALWARMCAGLREDGTEIEANDPNWPQLQQAAQAARTAPEVWLAQSEYYGDLAQETHFADRFCHWHALIWSKG
TREALRSYTTQI
>Mature_491_residues
TSLRLSNDTLSDLPKHITRPTYDRAALTAGILHIGLGNFHRAHQSWYLHRLMQQGEAQDWAIIGAGVRDYDAAMRARLKA
QDCLTTLIALSPDTTSAEVVGSMIDYLPIEADNGALIAAMARPEIRIVSMTVTEGGYYRDPVSGAFDDTHPDILHDIANP
STPRTVFGAIIAALALRRTAGVGPFSVQSCDNLQGNGDVTRQTVLGLARRSDPELASWIAAQCTFPNSMVDCIVPATGPA
EIALAAEMGVADQAPVTHEPYRQWVIEDNFCAGRPDWDKVGATFTNQVHAYETMKIRILNAGHQVIANAGELLSCETIAD
CMQDPDIRSMFEKVETQEIAPYVSPVPGITADAYVALIADRFSNPAIHDTTRRVAFDGSSRHAGFILPILRDALAARGPV
DGLALVEALWARMCAGLREDGTEIEANDPNWPQLQQAAQAARTAPEVWLAQSEYYGDLAQETHFADRFCHWHALIWSKGT
REALRSYTTQI

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=480, Percent_Identity=39.7916666666667, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1787823, Length=492, Percent_Identity=37.3983739837398, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788497, Length=457, Percent_Identity=35.8862144420131, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI48994885, Length=499, Percent_Identity=23.0460921843687, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790028, Length=198, Percent_Identity=29.2929292929293, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324401, Length=495, Percent_Identity=35.7575757575758, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6320765, Length=495, Percent_Identity=35.7575757575758, Blast_Score=338, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.67 [H]

Molecular weight: Translated: 53760; Mature: 53629

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLRLSNDTLSDLPKHITRPTYDRAALTAGILHIGLGNFHRAHQSWYLHRLMQQGEAQD
CCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
WAIIGAGVRDYDAAMRARLKAQDCLTTLIALSPDTTSAEVVGSMIDYLPIEADNGALIAA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEECCCCCEEEE
MARPEIRIVSMTVTEGGYYRDPVSGAFDDTHPDILHDIANPSTPRTVFGAIIAALALRRT
ECCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AGVGPFSVQSCDNLQGNGDVTRQTVLGLARRSDPELASWIAAQCTFPNSMVDCIVPATGP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHEEECCCCC
AEIALAAEMGVADQAPVTHEPYRQWVIEDNFCAGRPDWDKVGATFTNQVHAYETMKIRIL
CCEEEEHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHCCHHHCCEEEEEEEEEEEE
NAGHQVIANAGELLSCETIADCMQDPDIRSMFEKVETQEIAPYVSPVPGITADAYVALIA
CCCHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DRFSNPAIHDTTRRVAFDGSSRHAGFILPILRDALAARGPVDGLALVEALWARMCAGLRE
HHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
DGTEIEANDPNWPQLQQAAQAARTAPEVWLAQSEYYGDLAQETHFADRFCHWHALIWSKG
CCCEECCCCCCCHHHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TREALRSYTTQI
HHHHHHHHHCCC
>Mature Secondary Structure 
TSLRLSNDTLSDLPKHITRPTYDRAALTAGILHIGLGNFHRAHQSWYLHRLMQQGEAQD
CCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
WAIIGAGVRDYDAAMRARLKAQDCLTTLIALSPDTTSAEVVGSMIDYLPIEADNGALIAA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEECCCCCEEEE
MARPEIRIVSMTVTEGGYYRDPVSGAFDDTHPDILHDIANPSTPRTVFGAIIAALALRRT
ECCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AGVGPFSVQSCDNLQGNGDVTRQTVLGLARRSDPELASWIAAQCTFPNSMVDCIVPATGP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHEEECCCCC
AEIALAAEMGVADQAPVTHEPYRQWVIEDNFCAGRPDWDKVGATFTNQVHAYETMKIRIL
CCEEEEHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHCCHHHCCEEEEEEEEEEEE
NAGHQVIANAGELLSCETIADCMQDPDIRSMFEKVETQEIAPYVSPVPGITADAYVALIA
CCCHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DRFSNPAIHDTTRRVAFDGSSRHAGFILPILRDALAARGPVDGLALVEALWARMCAGLRE
HHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
DGTEIEANDPNWPQLQQAAQAARTAPEVWLAQSEYYGDLAQETHFADRFCHWHALIWSKG
CCCEECCCCCCCHHHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TREALRSYTTQI
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8254318 [H]