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The map label for this gene is eda [H]

Identifier: 99080216

GI number: 99080216

Start: 384038

End: 384685

Strand: Reverse

Name: eda [H]

Synonym: TM1040_0375

Alternate gene names: 99080216

Gene position: 384685-384038 (Counterclockwise)

Preceding gene: 99080217

Following gene: 99080215

Centisome position: 12.02

GC content: 66.36

Gene sequence:

>648_bases
ATGACACTTACCCCGCAACAGTCCAGCGCGAAGGCGCGCGAAATCTGCGCCCTCGCCCCGATCGTTCCGGTCCTCGTTGT
GCATGACGTCGCCCACGCCCGCCCCTTGGCCGAAGCACTGGTCGCTGGCGGTCTGCCCGCCCTCGAAGTCACCCTACGCA
CCCCGGCTGCACTTGATGTGATCCGCGAGATGTCCAAGGTCGAAGGCGGTGTTGTCGGTGCCGGGACGCTGGTCACGCCC
GCAGACGTGACCGCTGCTGTTGAAGCTGGCGCAAAGTTCGGTGTCTCCCCCGGCTCCACACCCCTGCTGCTTGATGCGGC
TCTGGCTGCGGATCTGCCGATGCTGCCGGGTGCGGCCACCGCTTCTGAGGCCATGACGCTCTTGGAGCGTGGCTATGACA
TGCTTAAATTCTTCCCCGCCGAAGCTTCCGGGGGCGCGCCCGCGCTCAAGGCCATCGGCGCGCCTTTGCCGCAGATTTCC
TTCTGCCCCACCGGGGGCGTCAGCCCCAGCAATGCCGAAAGCTACCTGGGCCTCTCCAACGTGGTCTGCGCAGGCGGCAG
TTGGGTTGCACCGGCCAAACTCGTCGAGGCAGGCGACTGGAAAGCCATTGAAGACCTGGCCCGCGAGGCAAGCACGCTCG
CCCGCTGA

Upstream 100 bases:

>100_bases
ACCTCTTATCAACACGGCACTGGGCGCGAGCTCTTTGCCCTGTTTCGCAGTTCCGTGACCTCTGCCGACACCGGCGCAAC
CGTATTTGGAGTTTGACCTC

Downstream 100 bases:

>100_bases
AGGAGCGATGACCGATCTTACAGAACTTGTACCGGGCGCCGCTAGCCAGCGGCGCCCGGTGGCATTTCTACAGACCCAGG
CTTGACCCGCCCCCAAAGCC

Product: 2-keto-3-deoxy-phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTLTPQQSSAKAREICALAPIVPVLVVHDVAHARPLAEALVAGGLPALEVTLRTPAALDVIREMSKVEGGVVGAGTLVTP
ADVTAAVEAGAKFGVSPGSTPLLLDAALAADLPMLPGAATASEAMTLLERGYDMLKFFPAEASGGAPALKAIGAPLPQIS
FCPTGGVSPSNAESYLGLSNVVCAGGSWVAPAKLVEAGDWKAIEDLAREASTLAR

Sequences:

>Translated_215_residues
MTLTPQQSSAKAREICALAPIVPVLVVHDVAHARPLAEALVAGGLPALEVTLRTPAALDVIREMSKVEGGVVGAGTLVTP
ADVTAAVEAGAKFGVSPGSTPLLLDAALAADLPMLPGAATASEAMTLLERGYDMLKFFPAEASGGAPALKAIGAPLPQIS
FCPTGGVSPSNAESYLGLSNVVCAGGSWVAPAKLVEAGDWKAIEDLAREASTLAR
>Mature_214_residues
TLTPQQSSAKAREICALAPIVPVLVVHDVAHARPLAEALVAGGLPALEVTLRTPAALDVIREMSKVEGGVVGAGTLVTPA
DVTAAVEAGAKFGVSPGSTPLLLDAALAADLPMLPGAATASEAMTLLERGYDMLKFFPAEASGGAPALKAIGAPLPQISF
CPTGGVSPSNAESYLGLSNVVCAGGSWVAPAKLVEAGDWKAIEDLAREASTLAR

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=180, Percent_Identity=52.7777777777778, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI48994954, Length=180, Percent_Identity=31.1111111111111, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 21587; Mature: 21456

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTPQQSSAKAREICALAPIVPVLVVHDVAHARPLAEALVAGGLPALEVTLRTPAALDV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
IREMSKVEGGVVGAGTLVTPADVTAAVEAGAKFGVSPGSTPLLLDAALAADLPMLPGAAT
HHHHHHHCCCEECCCCEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
ASEAMTLLERGYDMLKFFPAEASGGAPALKAIGAPLPQISFCPTGGVSPSNAESYLGLSN
HHHHHHHHHCCCHHHHEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHH
VVCAGGSWVAPAKLVEAGDWKAIEDLAREASTLAR
HEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLTPQQSSAKAREICALAPIVPVLVVHDVAHARPLAEALVAGGLPALEVTLRTPAALDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
IREMSKVEGGVVGAGTLVTPADVTAAVEAGAKFGVSPGSTPLLLDAALAADLPMLPGAAT
HHHHHHHCCCEECCCCEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
ASEAMTLLERGYDMLKFFPAEASGGAPALKAIGAPLPQISFCPTGGVSPSNAESYLGLSN
HHHHHHHHHCCCHHHHEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHH
VVCAGGSWVAPAKLVEAGDWKAIEDLAREASTLAR
HEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]