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The map label for this gene is gcvA [H]

Identifier: 99080173

GI number: 99080173

Start: 336588

End: 337442

Strand: Reverse

Name: gcvA [H]

Synonym: TM1040_0332

Alternate gene names: 99080173

Gene position: 337442-336588 (Counterclockwise)

Preceding gene: 99080176

Following gene: 99080171

Centisome position: 10.54

GC content: 65.85

Gene sequence:

>855_bases
ATGAATAAAGATCGCCTGCCTCCGCTTGAATGGATCCGCGCCTTCGAAGCCGCCGCGCGCCTTGGCAGCTTTACCGCCGC
CGCAGAGGAACTCGGGCTGACACAGGCCGCAGTGAGCCAGCGGATCGGACAGCTGGAGCAGCTTCTTGGCACGCCCCTTT
TTCACCGCCGGGCCCGCGCCATCTCGCTCACGGTAGAGGGCGAAGCCTGGGTGCCCCACGTCCGACTTGCGCTCGAAGGG
TTGCGCGACAGCACAGAGGCCGTCTTTGGCACCAGCCGCCAGCGTCTTACGCTTTCGGCCAGCCAGACCGTGATCGAGCT
GTGGCTCCTGCCCAGACTGGCCCGCCTGCAAACGCTGACCAATGCTGAAATCTCGGTGCAGTCCATGGTTGTTGGTGCCC
ATGACGCGCCTCAAGACGATGTGATCCGAATTCGCTATGGTTCAGGCAACTGGCCGCATGCCTACCGCGCCAGCCTCTAC
AAGGAAGAGATCGCACCCGTCGCAGCCCCCGCGCTTGTGGCGGGCGCGCCGCATTGGACCGGCTTGCCCCGCATCGCCTG
CGCGGGTCCGCGCCCGGGTTGGCACGCCTGGGCGGCACGCTTTGGTATCCCGACCACGCCCTTGCCAAGTCTCAGGTTCG
ACACTCACCTGTCGGCGCTTGGCGCGGCCAAGGCCGGGCTTGGTGTGTTTCTGGCCTCTCTGCCCCTCTGCGCAGAGGCT
CTGGACAGCGGCGCCTTGGTGCGGCTCAGCACGGCTTCCCTGCCCCATCACGAGACCTACTGGATGCTGACGAGCCCAGA
GGGTGTGTCGCGCGACCAATGGCAGGCGCTCACACAGGCGATCACCGAGATCTGA

Upstream 100 bases:

>100_bases
TAAGAGGCCCTTCGGGATTGATTTCGATTTCACTGGAGTCTCTCAGGGCCAATCAATAAACACAAATCAGGAATATATGA
CCTGAGACGTGGTTTTCCTT

Downstream 100 bases:

>100_bases
CCGCGACCGTTCAGGATGCCCATCGGGTCAGGCAGCCGCTAAGGGGCAACCGGCGCCTCTGCAGAGTCGCGCGCCCAGGC
GGCAAAACGCTCCACCTCCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNKDRLPPLEWIRAFEAAARLGSFTAAAEELGLTQAAVSQRIGQLEQLLGTPLFHRRARAISLTVEGEAWVPHVRLALEG
LRDSTEAVFGTSRQRLTLSASQTVIELWLLPRLARLQTLTNAEISVQSMVVGAHDAPQDDVIRIRYGSGNWPHAYRASLY
KEEIAPVAAPALVAGAPHWTGLPRIACAGPRPGWHAWAARFGIPTTPLPSLRFDTHLSALGAAKAGLGVFLASLPLCAEA
LDSGALVRLSTASLPHHETYWMLTSPEGVSRDQWQALTQAITEI

Sequences:

>Translated_284_residues
MNKDRLPPLEWIRAFEAAARLGSFTAAAEELGLTQAAVSQRIGQLEQLLGTPLFHRRARAISLTVEGEAWVPHVRLALEG
LRDSTEAVFGTSRQRLTLSASQTVIELWLLPRLARLQTLTNAEISVQSMVVGAHDAPQDDVIRIRYGSGNWPHAYRASLY
KEEIAPVAAPALVAGAPHWTGLPRIACAGPRPGWHAWAARFGIPTTPLPSLRFDTHLSALGAAKAGLGVFLASLPLCAEA
LDSGALVRLSTASLPHHETYWMLTSPEGVSRDQWQALTQAITEI
>Mature_284_residues
MNKDRLPPLEWIRAFEAAARLGSFTAAAEELGLTQAAVSQRIGQLEQLLGTPLFHRRARAISLTVEGEAWVPHVRLALEG
LRDSTEAVFGTSRQRLTLSASQTVIELWLLPRLARLQTLTNAEISVQSMVVGAHDAPQDDVIRIRYGSGNWPHAYRASLY
KEEIAPVAAPALVAGAPHWTGLPRIACAGPRPGWHAWAARFGIPTTPLPSLRFDTHLSALGAAKAGLGVFLASLPLCAEA
LDSGALVRLSTASLPHHETYWMLTSPEGVSRDQWQALTQAITEI

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=34.5238095238095, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786448, Length=245, Percent_Identity=33.469387755102, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=106, Percent_Identity=38.6792452830189, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30791; Mature: 30791

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDRLPPLEWIRAFEAAARLGSFTAAAEELGLTQAAVSQRIGQLEQLLGTPLFHRRARA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHCCCEE
ISLTVEGEAWVPHVRLALEGLRDSTEAVFGTSRQRLTLSASQTVIELWLLPRLARLQTLT
EEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
NAEISVQSMVVGAHDAPQDDVIRIRYGSGNWPHAYRASLYKEEIAPVAAPALVAGAPHWT
CCCEEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLPRIACAGPRPGWHAWAARFGIPTTPLPSLRFDTHLSALGAAKAGLGVFLASLPLCAEA
CCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSGALVRLSTASLPHHETYWMLTSPEGVSRDQWQALTQAITEI
HCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKDRLPPLEWIRAFEAAARLGSFTAAAEELGLTQAAVSQRIGQLEQLLGTPLFHRRARA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHCCCEE
ISLTVEGEAWVPHVRLALEGLRDSTEAVFGTSRQRLTLSASQTVIELWLLPRLARLQTLT
EEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
NAEISVQSMVVGAHDAPQDDVIRIRYGSGNWPHAYRASLYKEEIAPVAAPALVAGAPHWT
CCCEEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLPRIACAGPRPGWHAWAARFGIPTTPLPSLRFDTHLSALGAAKAGLGVFLASLPLCAEA
CCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSGALVRLSTASLPHHETYWMLTSPEGVSRDQWQALTQAITEI
HCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]