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The map label for this gene is potH [H]

Identifier: 99080165

GI number: 99080165

Start: 329049

End: 329936

Strand: Direct

Name: potH [H]

Synonym: TM1040_0324

Alternate gene names: 99080165

Gene position: 329049-329936 (Clockwise)

Preceding gene: 99080164

Following gene: 99080166

Centisome position: 10.28

GC content: 63.63

Gene sequence:

>888_bases
ATGACGTCTTATTTTTCTCCGGCACCCGCCTCGGTGCGCCGGACCCCGTTTCTGTTTGGCCGTTTCTGGCGGGCGCTCTG
GGTGCTGCCGCTGCCGGTCTGGCTGCTGCTGTTTTTTGCGGTGCCGCTAGGCCTGCTGGTGGCGATTTCCTTCTGGTCGG
TGGTGAACTACCGGCTCACACCTGACGCCACGCTCTCGGCCTGGCGCTATGTGCTGGGGCTCGAGTTCCTCTGGGCGAGC
TTCTGGCGCAGCTACGGCCTTGCCATGGCCAGCGCGGTCGCGATTTCGCTTTTGGCGTTTCCGGCGTCCTACGCGATGGC
CTTTGTGGTGGCACCGCGGCAGCGGCTGATCCTGCTGGGGCTGGCGATTGCACCGTTTTTCACAAGCTATCTGGTGCGGG
TTTACTCCTGGCAGGTCTTCCTGACCGAGGGCGGCGTGATCCCGTCGATTGTGCAAGCCCTGGGTGGCAGCGGCGAGAGC
CTGCTCAACACCTATTTCGCGCTGTTTCTGGGACATGCAACCCTGGCGCTTCCGGTGGTGCTGATCCTGCAGACCATCGC
GCTTGTGAATGTGGACCGGACCGGGATTTCTGCGGCGGCAAACCTTGGCGCACGTCCCGGAGCCATCCTGTTGCAGGTGA
TCCTGCCCGCCGCCCGGCCCGGACTCACGCTGGGGCTGCTGTTTGCGTTTCTGCTCTCCTATGCCGAGTTCGTCAGCGCG
TCTTATCTCGGGGGCGGCAGCTTTCAGACCCTCCCGATCCTCGTGACGGATCTGGTGCGGGCAGGCCAGCAATGGCCACG
CGCCGCCGTGGTGGCGCTGATGATGATCGCAAGCCTTTTGATCACGGCCTTTGTCACCGTCCTCTGGGCGTACCGCGAGA
AAGGATAA

Upstream 100 bases:

>100_bases
CAAGAACGCCTGATGCCTGCGTGAGCCCCTATCCGGGCCGTCTCTGAGCCCTGTTTGCCCCGCATGGGGGCCGGGGCGAC
CGCTTCTTGGAGATCTCGAT

Downstream 100 bases:

>100_bases
GCCATGCCTGTTCATCTGAAACATGCCCTTCCCAAAGCCTTCTGGATTGGCGGCCTCGTTCTGGTGTTCGTGCTGCTTTA
TGCCCCGATCCTGTCGGCCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTSYFSPAPASVRRTPFLFGRFWRALWVLPLPVWLLLFFAVPLGLLVAISFWSVVNYRLTPDATLSAWRYVLGLEFLWAS
FWRSYGLAMASAVAISLLAFPASYAMAFVVAPRQRLILLGLAIAPFFTSYLVRVYSWQVFLTEGGVIPSIVQALGGSGES
LLNTYFALFLGHATLALPVVLILQTIALVNVDRTGISAAANLGARPGAILLQVILPAARPGLTLGLLFAFLLSYAEFVSA
SYLGGGSFQTLPILVTDLVRAGQQWPRAAVVALMMIASLLITAFVTVLWAYREKG

Sequences:

>Translated_295_residues
MTSYFSPAPASVRRTPFLFGRFWRALWVLPLPVWLLLFFAVPLGLLVAISFWSVVNYRLTPDATLSAWRYVLGLEFLWAS
FWRSYGLAMASAVAISLLAFPASYAMAFVVAPRQRLILLGLAIAPFFTSYLVRVYSWQVFLTEGGVIPSIVQALGGSGES
LLNTYFALFLGHATLALPVVLILQTIALVNVDRTGISAAANLGARPGAILLQVILPAARPGLTLGLLFAFLLSYAEFVSA
SYLGGGSFQTLPILVTDLVRAGQQWPRAAVVALMMIASLLITAFVTVLWAYREKG
>Mature_294_residues
TSYFSPAPASVRRTPFLFGRFWRALWVLPLPVWLLLFFAVPLGLLVAISFWSVVNYRLTPDATLSAWRYVLGLEFLWASF
WRSYGLAMASAVAISLLAFPASYAMAFVVAPRQRLILLGLAIAPFFTSYLVRVYSWQVFLTEGGVIPSIVQALGGSGESL
LNTYFALFLGHATLALPVVLILQTIALVNVDRTGISAAANLGARPGAILLQVILPAARPGLTLGLLFAFLLSYAEFVSAS
YLGGGSFQTLPILVTDLVRAGQQWPRAAVVALMMIASLLITAFVTVLWAYREKG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=217, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787713, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32099; Mature: 31967

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYFSPAPASVRRTPFLFGRFWRALWVLPLPVWLLLFFAVPLGLLVAISFWSVVNYRLT
CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDATLSAWRYVLGLEFLWASFWRSYGLAMASAVAISLLAFPASYAMAFVVAPRQRLILLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAIAPFFTSYLVRVYSWQVFLTEGGVIPSIVQALGGSGESLLNTYFALFLGHATLALPVV
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LILQTIALVNVDRTGISAAANLGARPGAILLQVILPAARPGLTLGLLFAFLLSYAEFVSA
HHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SYLGGGSFQTLPILVTDLVRAGQQWPRAAVVALMMIASLLITAFVTVLWAYREKG
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSYFSPAPASVRRTPFLFGRFWRALWVLPLPVWLLLFFAVPLGLLVAISFWSVVNYRLT
CCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDATLSAWRYVLGLEFLWASFWRSYGLAMASAVAISLLAFPASYAMAFVVAPRQRLILLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAIAPFFTSYLVRVYSWQVFLTEGGVIPSIVQALGGSGESLLNTYFALFLGHATLALPVV
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LILQTIALVNVDRTGISAAANLGARPGAILLQVILPAARPGLTLGLLFAFLLSYAEFVSA
HHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SYLGGGSFQTLPILVTDLVRAGQQWPRAAVVALMMIASLLITAFVTVLWAYREKG
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]