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The map label for this gene is potC [H]

Identifier: 99080166

GI number: 99080166

Start: 329940

End: 330761

Strand: Direct

Name: potC [H]

Synonym: TM1040_0325

Alternate gene names: 99080166

Gene position: 329940-330761 (Clockwise)

Preceding gene: 99080165

Following gene: 99080167

Centisome position: 10.31

GC content: 60.71

Gene sequence:

>822_bases
ATGCCTGTTCATCTGAAACATGCCCTTCCCAAAGCCTTCTGGATTGGCGGCCTCGTTCTGGTGTTCGTGCTGCTTTATGC
CCCGATCCTGTCGGCCGTGATGTTCTCTTTCAGCGCCGATCGCTTCCCCAGCCTCAGCCTGTCGGGGTTCAGTTCCAAAT
GGTATGCGCAAGTCCTCAGCGACCGCGCCATGCTGGACGCCCTCTGGAACAGCCTGCGCGTGGCCATGCTCACAGCGCTC
ATCGCGACGGCGCTTGGATTTGCCGCGGCTTATGCAGATTATCGGTTCCGTTTTACCGGCCAGAGAGCCGTTCTGGCGCT
GGCCTTGTTACCGCCGACGGTGCCGCTGGTGATCCTGGGGCTTGCCATGCTCGCCTTTTTCTCTCGCATCGGGATCGCAG
GGACCTTGATGGCGGTGGTGGCCGCCCATGTGGTGATCGCGGCACCTTTTGCGATGGCGGTCTGCCGCCTGCGGCTGGCG
CAGCTTGATGACAGCCTTGAAATTGCGGCTCAGAACCTCGGCGCGCTGCCGCATTCTGCGCTCACCCGGATCGTTCTGCC
CCATACTCTGCCCGCGATCTTTGCAGCCTTTCTCATCACCTTTGCCGTGTCCTTTGATGAATATGTCATCTCCTGGTTCA
CCGGGGGGCTCAACCAGACCATCCCGGTTGCGGTTCTGACCACGCTGCAGGGGCAGGTGGACCCCACGATCCATGTGATC
GGCACATTGAGTTTTTCCGTCACGCTGACGCTGGTTCTGATCGCGCTTGGCGTGGTCCTGACCCAGAGCAGCGGCGCCAT
GTCCAAGGAGGAGACAACATGA

Upstream 100 bases:

>100_bases
ATGGCCACGCGCCGCCGTGGTGGCGCTGATGATGATCGCAAGCCTTTTGATCACGGCCTTTGTCACCGTCCTCTGGGCGT
ACCGCGAGAAAGGATAAGCC

Downstream 100 bases:

>100_bases
CCCACTCACATCTGAAACTGGATGGGCTGGCCAAGTCCTATGGCAGCTTTGTTGCCGTCGAGGAGGTCTCGCTGGATATT
GCCGAAGGGGAGTTCATCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPVHLKHALPKAFWIGGLVLVFVLLYAPILSAVMFSFSADRFPSLSLSGFSSKWYAQVLSDRAMLDALWNSLRVAMLTAL
IATALGFAAAYADYRFRFTGQRAVLALALLPPTVPLVILGLAMLAFFSRIGIAGTLMAVVAAHVVIAAPFAMAVCRLRLA
QLDDSLEIAAQNLGALPHSALTRIVLPHTLPAIFAAFLITFAVSFDEYVISWFTGGLNQTIPVAVLTTLQGQVDPTIHVI
GTLSFSVTLTLVLIALGVVLTQSSGAMSKEETT

Sequences:

>Translated_273_residues
MPVHLKHALPKAFWIGGLVLVFVLLYAPILSAVMFSFSADRFPSLSLSGFSSKWYAQVLSDRAMLDALWNSLRVAMLTAL
IATALGFAAAYADYRFRFTGQRAVLALALLPPTVPLVILGLAMLAFFSRIGIAGTLMAVVAAHVVIAAPFAMAVCRLRLA
QLDDSLEIAAQNLGALPHSALTRIVLPHTLPAIFAAFLITFAVSFDEYVISWFTGGLNQTIPVAVLTTLQGQVDPTIHVI
GTLSFSVTLTLVLIALGVVLTQSSGAMSKEETT
>Mature_272_residues
PVHLKHALPKAFWIGGLVLVFVLLYAPILSAVMFSFSADRFPSLSLSGFSSKWYAQVLSDRAMLDALWNSLRVAMLTALI
ATALGFAAAYADYRFRFTGQRAVLALALLPPTVPLVILGLAMLAFFSRIGIAGTLMAVVAAHVVIAAPFAMAVCRLRLAQ
LDDSLEIAAQNLGALPHSALTRIVLPHTLPAIFAAFLITFAVSFDEYVISWFTGGLNQTIPVAVLTTLQGQVDPTIHVIG
TLSFSVTLTLVLIALGVVLTQSSGAMSKEETT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=231, Percent_Identity=27.7056277056277, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787081, Length=234, Percent_Identity=35.042735042735, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787714, Length=248, Percent_Identity=31.0483870967742, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29145; Mature: 29014

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PVHLKHALPKAFWIGGLVLVFVLLYAPILSAVMFSFSADRFPSLSLSGFSSKWYAQVLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
DRAMLDALWNSLRVAMLTALIATALGFAAAYADYRFRFTGQRAVLALALLPPTVPLVILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHHH
LAMLAFFSRIGIAGTLMAVVAAHVVIAAPFAMAVCRLRLAQLDDSLEIAAQNLGALPHSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHH
LTRIVLPHTLPAIFAAFLITFAVSFDEYVISWFTGGLNQTIPVAVLTTLQGQVDPTIHVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEE
GTLSFSVTLTLVLIALGVVLTQSSGAMSKEETT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]