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The map label for this gene is potD [C]

Identifier: 99080164

GI number: 99080164

Start: 327846

End: 328961

Strand: Direct

Name: potD [C]

Synonym: TM1040_0323

Alternate gene names: 99080164

Gene position: 327846-328961 (Clockwise)

Preceding gene: 99080162

Following gene: 99080165

Centisome position: 10.24

GC content: 63.53

Gene sequence:

>1116_bases
ATGACACTAAATATTATCACCACCCGCCGGCGTCTGATCAAGGGGGGGATCGCAGCAGCGGCCACCCTTGCGGCCCCGTC
CGTCCTGCGTGCCCAAGGGCGTCGCGATCTGTCGATGCTGACATGGGCCGGCCATGCGGACCCGGATGTGATCGGCGCGT
TTGAAGAGGCGCATAACGTGCGCATCCGCGCCAAGGAATATGTCGGCGGCGATCAGATGCTGGCGCAGATCGCGCAGTCC
CCGGCGGGCACTTATGATGTGATCCTGTCGGATGCGGAATATGTGGTGCAGCTGCAGCAAGCCGGGTTCATCGACCCGCT
CTCGCCCGAGGATTATCCGTTTGACGACTTTTTCCCCGAGTTCCAGAATTTCCGTGGGCATTGGTTCGACGGCCAGCCCT
ACGCGGTGTTTCTGAGGTTCGGCTTTCTGGGCCTGTCGCATCATCGCGGCGCCATGAGCGAGGCGGAGGCGCGCAGCTAT
CGTAGCATGTGGGAGACCCGCCACGAGGGGCGGATCGGCCATTTTGACTGGCATCTGCCCAACCTGGGGGCCCTGAGCCT
CATGAATGGCAACGCGGCGCCCTTTGATCTCGGTGCGGGAGATTGGGCGGTGCTCACAGAGACGATGATGTCGCTGCGCC
CGCAGGTGCGCGGGTTCTATGATTATGGCGGCATGCTGTCGGCGCTGCGGTCCGGAGAGGTTACCGCGATCCCCGGCATT
GGTGACTGGATCACGGGCGTCCTTGCCCGAGATGGTGCCGATGTCACCACGACTATCCCCGAGGAAGGGGGACTGCAGTG
GACCGAGTCGCTGTCGATTGGCACCCGCTCCCGGAACCCGGATCTGGCGCGTCAGTTCATCCAGTACATGCTCTCGCCCG
ACGGGCAGGTCAAAACCGCGACGCTCAAGGCCTATCCGGCGATGATCCCGACGCGGTCGGGCTGGGCGCGGTTGAACGCA
GAGCAACCGGGCGAGGCCAAACGTCAGAACATGGTGCTGAACGGCCCCAATGCGCTGGATGAAATCCGCGCCGGGCGGAT
CCGTCTGCGCGAGCTTCCGGTACAGCAATCGCTGGAAACCTGGAACGACGCCTGGACCCGGTACAAGAACGCCTGA

Upstream 100 bases:

>100_bases
GCCTCGGGTGAGAAAAGAAATCTCAGCATGGGCTGACGAATGGTCATTTGTTTTTCGGCCTCCTCTTTCTGAAGCTTGTG
GAATGAAAAGGGAGGATAAA

Downstream 100 bases:

>100_bases
TGCCTGCGTGAGCCCCTATCCGGGCCGTCTCTGAGCCCTGTTTGCCCCGCATGGGGGCCGGGGCGACCGCTTCTTGGAGA
TCTCGATATGACGTCTTATT

Product: twin-arginine translocation pathway signal

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTLNIITTRRRLIKGGIAAAATLAAPSVLRAQGRRDLSMLTWAGHADPDVIGAFEEAHNVRIRAKEYVGGDQMLAQIAQS
PAGTYDVILSDAEYVVQLQQAGFIDPLSPEDYPFDDFFPEFQNFRGHWFDGQPYAVFLRFGFLGLSHHRGAMSEAEARSY
RSMWETRHEGRIGHFDWHLPNLGALSLMNGNAAPFDLGAGDWAVLTETMMSLRPQVRGFYDYGGMLSALRSGEVTAIPGI
GDWITGVLARDGADVTTTIPEEGGLQWTESLSIGTRSRNPDLARQFIQYMLSPDGQVKTATLKAYPAMIPTRSGWARLNA
EQPGEAKRQNMVLNGPNALDEIRAGRIRLRELPVQQSLETWNDAWTRYKNA

Sequences:

>Translated_371_residues
MTLNIITTRRRLIKGGIAAAATLAAPSVLRAQGRRDLSMLTWAGHADPDVIGAFEEAHNVRIRAKEYVGGDQMLAQIAQS
PAGTYDVILSDAEYVVQLQQAGFIDPLSPEDYPFDDFFPEFQNFRGHWFDGQPYAVFLRFGFLGLSHHRGAMSEAEARSY
RSMWETRHEGRIGHFDWHLPNLGALSLMNGNAAPFDLGAGDWAVLTETMMSLRPQVRGFYDYGGMLSALRSGEVTAIPGI
GDWITGVLARDGADVTTTIPEEGGLQWTESLSIGTRSRNPDLARQFIQYMLSPDGQVKTATLKAYPAMIPTRSGWARLNA
EQPGEAKRQNMVLNGPNALDEIRAGRIRLRELPVQQSLETWNDAWTRYKNA
>Mature_370_residues
TLNIITTRRRLIKGGIAAAATLAAPSVLRAQGRRDLSMLTWAGHADPDVIGAFEEAHNVRIRAKEYVGGDQMLAQIAQSP
AGTYDVILSDAEYVVQLQQAGFIDPLSPEDYPFDDFFPEFQNFRGHWFDGQPYAVFLRFGFLGLSHHRGAMSEAEARSYR
SMWETRHEGRIGHFDWHLPNLGALSLMNGNAAPFDLGAGDWAVLTETMMSLRPQVRGFYDYGGMLSALRSGEVTAIPGIG
DWITGVLARDGADVTTTIPEEGGLQWTESLSIGTRSRNPDLARQFIQYMLSPDGQVKTATLKAYPAMIPTRSGWARLNAE
QPGEAKRQNMVLNGPNALDEIRAGRIRLRELPVQQSLETWNDAWTRYKNA

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41185; Mature: 41054

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNIITTRRRLIKGGIAAAATLAAPSVLRAQGRRDLSMLTWAGHADPDVIGAFEEAHNV
CEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCE
RIRAKEYVGGDQMLAQIAQSPAGTYDVILSDAEYVVQLQQAGFIDPLSPEDYPFDDFFPE
EEEEHHHCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FQNFRGHWFDGQPYAVFLRFGFLGLSHHRGAMSEAEARSYRSMWETRHEGRIGHFDWHLP
HHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCC
NLGALSLMNGNAAPFDLGAGDWAVLTETMMSLRPQVRGFYDYGGMLSALRSGEVTAIPGI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCEEECCCC
GDWITGVLARDGADVTTTIPEEGGLQWTESLSIGTRSRNPDLARQFIQYMLSPDGQVKTA
HHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
TLKAYPAMIPTRSGWARLNAEQPGEAKRQNMVLNGPNALDEIRAGRIRLRELPVQQSLET
EEEECCCCCCCCCCCEECCCCCCCCHHHCCEEECCCCHHHHHHHCCEEHHHCCHHHHHHH
WNDAWTRYKNA
HHHHHHHHCCC
>Mature Secondary Structure 
TLNIITTRRRLIKGGIAAAATLAAPSVLRAQGRRDLSMLTWAGHADPDVIGAFEEAHNV
EEEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCE
RIRAKEYVGGDQMLAQIAQSPAGTYDVILSDAEYVVQLQQAGFIDPLSPEDYPFDDFFPE
EEEEHHHCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FQNFRGHWFDGQPYAVFLRFGFLGLSHHRGAMSEAEARSYRSMWETRHEGRIGHFDWHLP
HHCCCCCCCCCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCC
NLGALSLMNGNAAPFDLGAGDWAVLTETMMSLRPQVRGFYDYGGMLSALRSGEVTAIPGI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCEEECCCC
GDWITGVLARDGADVTTTIPEEGGLQWTESLSIGTRSRNPDLARQFIQYMLSPDGQVKTA
HHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
TLKAYPAMIPTRSGWARLNAEQPGEAKRQNMVLNGPNALDEIRAGRIRLRELPVQQSLET
EEEECCCCCCCCCCCEECCCCCCCCHHHCCEEECCCCHHHHHHHCCEEHHHCCHHHHHHH
WNDAWTRYKNA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA