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The map label for this gene is engD [H]

Identifier: 99080065

GI number: 99080065

Start: 239155

End: 240252

Strand: Direct

Name: engD [H]

Synonym: TM1040_0224

Alternate gene names: 99080065

Gene position: 239155-240252 (Clockwise)

Preceding gene: 99080062

Following gene: 99080066

Centisome position: 7.47

GC content: 59.02

Gene sequence:

>1098_bases
ATGGGCTTTAAAATGGGAATCGTGGGTCTGCCCAATGTGGGCAAGTCGACCCTGTTCAACGCGCTGACCAAAACCGCCTC
GGCGCAGGCGGCAAATTTTCCGTTCTGTACGATCGAACCGAACGTGGGTGAGGTGGGCGTTCCGGACGCGCGTCTCGACA
AATTGGCGGCGATTGCGCAGTCCAAACAGATCATCCCAACCCGCATGACGTTTGTGGATATTGCTGGCCTCGTCAAAGGC
GCCTCAAAGGGCGAAGGTCTGGGCAACCAGTTCCTTGCCAATATCCGTGAGGTGGACGCAATTGCCCATGTTTTACGGTG
CTTTGAGGACGGTGACGTTACCCATGTCGATGGTCGCGTTGATCCGGTTGCGGACGCCGATACCATCGAAACCGAGCTGA
TGCTGGCGGATCTTGAGAGCATCGAGAAACGCCGCGCCAACCTCGTACGCAAGCTCAAGGGCAACGACAAGGAAGCCCAG
CAGCAGGACCGCCTGCTCGCAGCGGCGCAGGCCATGCTCGAAGATGGCAAACCAGCCCGTCTGGTTGAGGTCGACGCAGA
GGACCAGAAGGCCTGGACCATGCTGCAACTGCTGACCACAAAGCCGGTGCTTTACGTCTGCAATGTGGGTGAAAGCGAGA
GCGTCGAAGGCAACGCACATTCCGCCAAAGTTGCCGAGATGGCCGCGGCTCAGGGTAACGCGCATGTGATCATTTCGGCG
CAGATCGAAGAGGAAATCAGCCAGCTTGAGCCCGAAGAAGCGCAGATGTTCCTCGATGAGATGGGTCTCGCAGAAGCCGG
TCTCGACCGCCTGATCCGCGCCGGTTACGAGCTCTTGCATCTGGAAACCTATTTCACGGTCGGCCCCAAGGAAGCGCGCG
CCTGGACCATTCGCTCGGGCACCGCTGCGCCCCAGGCGGCAGGCGTTATCCACGGCGATTTTGAAAAGGGTTTCATCCGC
GCGGAGACCATCGCCTATGACGACTACATCGCTTGCGGCGGTGAATCCGGCGCCAAAGAAGCGGGCAAGATGCGCGCCGA
GGGCAAGAGCTACATCGTCAAGGATGGCGATGTGATGCACTTCTTGTTCAACACCTGA

Upstream 100 bases:

>100_bases
ATTTCAATAAGCGCCGCGTGCGTGTTGCAACGGCTGCGTGCTTGCGAAAACGGCGATTTGAGCCTAAGTGCTGGCTCCCA
AATGGCATCAGGAGGCCGGT

Downstream 100 bases:

>100_bases
TCATCCGCATCGTCACAAGTTCAGAATGCCCGTCTTCGAAGGAAGGCGGGCATTTTTGCGAAACAGTTGATGGATAATCG
AGAGGCTGGCAGGCTTGAGT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MGFKMGIVGLPNVGKSTLFNALTKTASAQAANFPFCTIEPNVGEVGVPDARLDKLAAIAQSKQIIPTRMTFVDIAGLVKG
ASKGEGLGNQFLANIREVDAIAHVLRCFEDGDVTHVDGRVDPVADADTIETELMLADLESIEKRRANLVRKLKGNDKEAQ
QQDRLLAAAQAMLEDGKPARLVEVDAEDQKAWTMLQLLTTKPVLYVCNVGESESVEGNAHSAKVAEMAAAQGNAHVIISA
QIEEEISQLEPEEAQMFLDEMGLAEAGLDRLIRAGYELLHLETYFTVGPKEARAWTIRSGTAAPQAAGVIHGDFEKGFIR
AETIAYDDYIACGGESGAKEAGKMRAEGKSYIVKDGDVMHFLFNT

Sequences:

>Translated_365_residues
MGFKMGIVGLPNVGKSTLFNALTKTASAQAANFPFCTIEPNVGEVGVPDARLDKLAAIAQSKQIIPTRMTFVDIAGLVKG
ASKGEGLGNQFLANIREVDAIAHVLRCFEDGDVTHVDGRVDPVADADTIETELMLADLESIEKRRANLVRKLKGNDKEAQ
QQDRLLAAAQAMLEDGKPARLVEVDAEDQKAWTMLQLLTTKPVLYVCNVGESESVEGNAHSAKVAEMAAAQGNAHVIISA
QIEEEISQLEPEEAQMFLDEMGLAEAGLDRLIRAGYELLHLETYFTVGPKEARAWTIRSGTAAPQAAGVIHGDFEKGFIR
AETIAYDDYIACGGESGAKEAGKMRAEGKSYIVKDGDVMHFLFNT
>Mature_364_residues
GFKMGIVGLPNVGKSTLFNALTKTASAQAANFPFCTIEPNVGEVGVPDARLDKLAAIAQSKQIIPTRMTFVDIAGLVKGA
SKGEGLGNQFLANIREVDAIAHVLRCFEDGDVTHVDGRVDPVADADTIETELMLADLESIEKRRANLVRKLKGNDKEAQQ
QDRLLAAAQAMLEDGKPARLVEVDAEDQKAWTMLQLLTTKPVLYVCNVGESESVEGNAHSAKVAEMAAAQGNAHVIISAQ
IEEEISQLEPEEAQMFLDEMGLAEAGLDRLIRAGYELLHLETYFTVGPKEARAWTIRSGTAAPQAAGVIHGDFEKGFIRA
ETIAYDDYIACGGESGAKEAGKMRAEGKSYIVKDGDVMHFLFNT

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=374, Percent_Identity=43.8502673796791, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI58761502, Length=220, Percent_Identity=38.6363636363636, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=58.2417582417582, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1789574, Length=229, Percent_Identity=26.6375545851528, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509631, Length=368, Percent_Identity=43.2065217391304, Blast_Score=286, Evalue=9e-78,
Organism=Saccharomyces cerevisiae, GI6319499, Length=369, Percent_Identity=40.1084010840108, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321773, Length=378, Percent_Identity=36.7724867724868, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321649, Length=159, Percent_Identity=36.4779874213836, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=42.6630434782609, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=42.6630434782609, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=42.6630434782609, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=40, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39350; Mature: 39219

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKMGIVGLPNVGKSTLFNALTKTASAQAANFPFCTIEPNVGEVGVPDARLDKLAAIAQ
CCEEECEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
SKQIIPTRMTFVDIAGLVKGASKGEGLGNQFLANIREVDAIAHVLRCFEDGDVTHVDGRV
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DPVADADTIETELMLADLESIEKRRANLVRKLKGNDKEAQQQDRLLAAAQAMLEDGKPAR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LVEVDAEDQKAWTMLQLLTTKPVLYVCNVGESESVEGNAHSAKVAEMAAAQGNAHVIISA
EEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
QIEEEISQLEPEEAQMFLDEMGLAEAGLDRLIRAGYELLHLETYFTVGPKEARAWTIRSG
HHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHCCHHEEEEEEEEEECCCCCCEEEEECC
TAAPQAAGVIHGDFEKGFIRAETIAYDDYIACGGESGAKEAGKMRAEGKSYIVKDGDVMH
CCCCCCCCEEECCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHCCCCEEEEECCCEEE
FLFNT
EEECC
>Mature Secondary Structure 
GFKMGIVGLPNVGKSTLFNALTKTASAQAANFPFCTIEPNVGEVGVPDARLDKLAAIAQ
CEEECEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
SKQIIPTRMTFVDIAGLVKGASKGEGLGNQFLANIREVDAIAHVLRCFEDGDVTHVDGRV
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DPVADADTIETELMLADLESIEKRRANLVRKLKGNDKEAQQQDRLLAAAQAMLEDGKPAR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LVEVDAEDQKAWTMLQLLTTKPVLYVCNVGESESVEGNAHSAKVAEMAAAQGNAHVIISA
EEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
QIEEEISQLEPEEAQMFLDEMGLAEAGLDRLIRAGYELLHLETYFTVGPKEARAWTIRSG
HHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHCCHHEEEEEEEEEECCCCCCEEEEECC
TAAPQAAGVIHGDFEKGFIRAETIAYDDYIACGGESGAKEAGKMRAEGKSYIVKDGDVMH
CCCCCCCCEEECCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHCCCCEEEEECCCEEE
FLFNT
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]