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The map label for this gene is trpB

Identifier: 99080047

GI number: 99080047

Start: 223721

End: 224980

Strand: Direct

Name: trpB

Synonym: TM1040_0206

Alternate gene names: 99080047

Gene position: 223721-224980 (Clockwise)

Preceding gene: 99080046

Following gene: 99080049

Centisome position: 6.99

GC content: 60.63

Gene sequence:

>1260_bases
ATGGCCGAAGATCTCATCAATAGCTTCATGAACGGGCCGGACGAAAACGGTCGGTTTGGCATCTTTGGCGGCCGCTTTGT
CAGCGAGACACTGATGCCGCTGATCCTGAGCCTCGAAGAGGAATACGAAAAGGCCAAGGTGGATCCGGATTTCTGGGCGG
AAATGGATGATCTGTGGAAGAACTATGTGGGCCGCCCGAGCCCGCTCTATTTCGCCGAGCGCCTGACCAATCATCTGGGC
GGCGCCAAGGTCTACATGAAGCGTGACGAGCTCAATCACACCGGCGCGCATAAAGTGAACAATGTGCTGGGTCAGATCCT
GCTCGCCCGCCGCATGGGCAAGACTAGGATCATCGCCGAAACCGGTGCTGGCCAGCATGGGGTTGCGACCGCCACTGTCT
GTGCCAAGTTTGGTCTGAAATGCGTGGTCTACATGGGCGCTCATGACGTACGTCGACAGGCGCCCAACGTGTTCCGGATG
CGTCTTCTTGGCGCTGAGGTGATCCCAGTCACCTCTGGCCGTGGCACGCTCAAGGATGCGATGAACGACGCGCTGCGGGA
CTGGGTCACCAATGTGCGCGACACATTCTACTGCATCGGCACCGTTGCGGGCCCGCACCCCTATCCGGCTATGGTGCGCG
ATTTCCAGTCCGTGATCGGCAAGGAAGTGCGCTGGCAGCTTGCAGAGCAGGAAGGCGAGGGCCGGTTGCCGGACACGGTG
ATTGCGGCTATCGGAGGGGGCTCCAACGCGATGGGCCTGTTCCACCCGTTCCTCGATGACCCTTCGGTCAATATCATTGG
CGTTGAGGCCGGCGGCAAAGGTGTGGATGAGAAAATGGAGCATTGCGCCTCATTGACAGGCGGCCGGCCGGGCGTGCTGC
ACGGCAACCGGACCTATCTGCTGCAGGACGATGACGGCCAAATCCTCGAAGGCTTCTCGATTTCGGCGGGCCTGGATTAC
CCGGGGATCGGGCCGGAGCATGCCTGGCTGCATGAGACCGGGCGCGCGCAATACGTGTCCATCACCGACAAGGAAGCCCT
CGAGGCGTTCCAGCTGTCCTGCGCGATGGAGGGGATTATCCCGGCGCTCGAGCCGAGCCACGCGCTCGCTCATGTCACTA
AGATCGCACCAGAACTGCCCAAGGACCACATCATCGTGATGAACATGTGTGGGCGTGGCGACAAGGACATCTTTACCGTA
GCCCGCCACCTCGGGTTTGATATGTCAGACACCGAAGAGGGCCGCGACCTCGAAGAGTGA

Upstream 100 bases:

>100_bases
TCGCTTTCCACCGGTCGAAGGCGGAAATTGGCGAGAAACCTGTTTCTCTTGTTCTAAATGCACTTTAGAACCAGCGCATA
CGGAAGACGGAGACATCACC

Downstream 100 bases:

>100_bases
GCGCAGGCTTTTTCGGAAAGATCCAAGTGGAAGGCGCGTCCTGAGGGGTGCGCCTTTTCTTTTTCTGGCGTGCGCCGGGA
CAGCCGTCGCGTCGTGGATC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAEDLINSFMNGPDENGRFGIFGGRFVSETLMPLILSLEEEYEKAKVDPDFWAEMDDLWKNYVGRPSPLYFAERLTNHLG
GAKVYMKRDELNHTGAHKVNNVLGQILLARRMGKTRIIAETGAGQHGVATATVCAKFGLKCVVYMGAHDVRRQAPNVFRM
RLLGAEVIPVTSGRGTLKDAMNDALRDWVTNVRDTFYCIGTVAGPHPYPAMVRDFQSVIGKEVRWQLAEQEGEGRLPDTV
IAAIGGGSNAMGLFHPFLDDPSVNIIGVEAGGKGVDEKMEHCASLTGGRPGVLHGNRTYLLQDDDGQILEGFSISAGLDY
PGIGPEHAWLHETGRAQYVSITDKEALEAFQLSCAMEGIIPALEPSHALAHVTKIAPELPKDHIIVMNMCGRGDKDIFTV
ARHLGFDMSDTEEGRDLEE

Sequences:

>Translated_419_residues
MAEDLINSFMNGPDENGRFGIFGGRFVSETLMPLILSLEEEYEKAKVDPDFWAEMDDLWKNYVGRPSPLYFAERLTNHLG
GAKVYMKRDELNHTGAHKVNNVLGQILLARRMGKTRIIAETGAGQHGVATATVCAKFGLKCVVYMGAHDVRRQAPNVFRM
RLLGAEVIPVTSGRGTLKDAMNDALRDWVTNVRDTFYCIGTVAGPHPYPAMVRDFQSVIGKEVRWQLAEQEGEGRLPDTV
IAAIGGGSNAMGLFHPFLDDPSVNIIGVEAGGKGVDEKMEHCASLTGGRPGVLHGNRTYLLQDDDGQILEGFSISAGLDY
PGIGPEHAWLHETGRAQYVSITDKEALEAFQLSCAMEGIIPALEPSHALAHVTKIAPELPKDHIIVMNMCGRGDKDIFTV
ARHLGFDMSDTEEGRDLEE
>Mature_418_residues
AEDLINSFMNGPDENGRFGIFGGRFVSETLMPLILSLEEEYEKAKVDPDFWAEMDDLWKNYVGRPSPLYFAERLTNHLGG
AKVYMKRDELNHTGAHKVNNVLGQILLARRMGKTRIIAETGAGQHGVATATVCAKFGLKCVVYMGAHDVRRQAPNVFRMR
LLGAEVIPVTSGRGTLKDAMNDALRDWVTNVRDTFYCIGTVAGPHPYPAMVRDFQSVIGKEVRWQLAEQEGEGRLPDTVI
AAIGGGSNAMGLFHPFLDDPSVNIIGVEAGGKGVDEKMEHCASLTGGRPGVLHGNRTYLLQDDDGQILEGFSISAGLDYP
GIGPEHAWLHETGRAQYVSITDKEALEAFQLSCAMEGIIPALEPSHALAHVTKIAPELPKDHIIVMNMCGRGDKDIFTVA
RHLGFDMSDTEEGRDLEE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=386, Percent_Identity=58.2901554404145, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321412, Length=404, Percent_Identity=55.1980198019802, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_SILST (Q1GK77)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612201.1
- ProteinModelPortal:   Q1GK77
- SMR:   Q1GK77
- STRING:   Q1GK77
- GeneID:   4078654
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0206
- NMPDR:   fig|292414.1.peg.2598
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q1GK77
- ProtClustDB:   PRK04346
- BioCyc:   SSP292414:TM1040_0206-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 45919; Mature: 45788

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDLINSFMNGPDENGRFGIFGGRFVSETLMPLILSLEEEYEKAKVDPDFWAEMDDLWK
CHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NYVGRPSPLYFAERLTNHLGGAKVYMKRDELNHTGAHKVNNVLGQILLARRMGKTRIIAE
HHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGQHGVATATVCAKFGLKCVVYMGAHDVRRQAPNVFRMRLLGAEVIPVTSGRGTLKDA
CCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHHH
MNDALRDWVTNVRDTFYCIGTVAGPHPYPAMVRDFQSVIGKEVRWQLAEQEGEGRLPDTV
HHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHE
IAAIGGGSNAMGLFHPFLDDPSVNIIGVEAGGKGVDEKMEHCASLTGGRPGVLHGNRTYL
EEEECCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCEEEE
LQDDDGQILEGFSISAGLDYPGIGPEHAWLHETGRAQYVSITDKEALEAFQLSCAMEGII
EECCCCCEECCEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCC
PALEPSHALAHVTKIAPELPKDHIIVMNMCGRGDKDIFTVARHLGFDMSDTEEGRDLEE
CCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEDLINSFMNGPDENGRFGIFGGRFVSETLMPLILSLEEEYEKAKVDPDFWAEMDDLWK
HHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NYVGRPSPLYFAERLTNHLGGAKVYMKRDELNHTGAHKVNNVLGQILLARRMGKTRIIAE
HHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGQHGVATATVCAKFGLKCVVYMGAHDVRRQAPNVFRMRLLGAEVIPVTSGRGTLKDA
CCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHCCHHHHHHHHCCEEEEECCCCCHHHHH
MNDALRDWVTNVRDTFYCIGTVAGPHPYPAMVRDFQSVIGKEVRWQLAEQEGEGRLPDTV
HHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHE
IAAIGGGSNAMGLFHPFLDDPSVNIIGVEAGGKGVDEKMEHCASLTGGRPGVLHGNRTYL
EEEECCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCEEEE
LQDDDGQILEGFSISAGLDYPGIGPEHAWLHETGRAQYVSITDKEALEAFQLSCAMEGII
EECCCCCEECCEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCC
PALEPSHALAHVTKIAPELPKDHIIVMNMCGRGDKDIFTVARHLGFDMSDTEEGRDLEE
CCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA