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The map label for this gene is trpF

Identifier: 99080045

GI number: 99080045

Start: 221741

End: 222388

Strand: Direct

Name: trpF

Synonym: TM1040_0204

Alternate gene names: 99080045

Gene position: 221741-222388 (Clockwise)

Preceding gene: 99080044

Following gene: 99080046

Centisome position: 6.93

GC content: 59.72

Gene sequence:

>648_bases
ATGGCAGATATTCGGGTCAAGATTTGCGGCATGAAGACGCGCGCCGACATGGAGGCTGCGGCAGCTGCAGGCGCGGCCTA
TGTGGGTCTGAATTTCTACGCCAAATCAGCACGTTCCGTCACGATTGCTCAAGCTGCCGCACTTGCTTCAGACGCGCCTG
TGGGACTTGCGAAGGTGGGGCTCGTCGTCAACCCCACCGATGCAGATCTCGATGCGATTACCGGATCCGTCCCGCTCGAT
ATGATTCAGCTTCACGGTCAGGAGAGCGTGGAGCGCGTGGCCGAAATCAAGACACGCTACGGGTTGCCCGTCATGAAGGT
GATCGGCGTCGCCGAGGCCGCAGATCTGGATCCCATTGATCTCTACGCGCAAGTTGCCGATCAACTGATGGTCGATGCCA
AGGCCCCGAAAGGGGCGAAACTCCCCGGTGGAAATGGGATCTCCTTTGACTGGCAGTTGCTGGCCAGTAAAAAATACTGG
CAGGCACCTTGGATGTTGGCTGGTGGTCTGACGCCTGAGAATGTCGCTGAGGCGATCCGGAAAACCGGCGCGCGCCAGGT
GGATGTCGCCTCTGGCGTTGAGAGCGCTCCGGCACAGAAAGATCCGGATCTGATGCGCGCCTTTGTCGAGGCCGCGCAAG
CCGTCTGA

Upstream 100 bases:

>100_bases
GGTGAAGTCAAAAAACTGTCGCGCGAAGTGAAACGTCTCAAGCGTCAAAAACACGAAGGCAAGGACGAGGTTCTCGCCCT
TCTGGACGACGCCTCCTGAT

Downstream 100 bases:

>100_bases
CCGGTCCTACCGTTTCAATTTCAGCCCTTTCTTGACCCATCTGAGTGCCCTCAGGCGCAACAGGCGTCTTTTACGCCTGC
GATAGCTGGCAAGGCGACGT

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MADIRVKICGMKTRADMEAAAAAGAAYVGLNFYAKSARSVTIAQAAALASDAPVGLAKVGLVVNPTDADLDAITGSVPLD
MIQLHGQESVERVAEIKTRYGLPVMKVIGVAEAADLDPIDLYAQVADQLMVDAKAPKGAKLPGGNGISFDWQLLASKKYW
QAPWMLAGGLTPENVAEAIRKTGARQVDVASGVESAPAQKDPDLMRAFVEAAQAV

Sequences:

>Translated_215_residues
MADIRVKICGMKTRADMEAAAAAGAAYVGLNFYAKSARSVTIAQAAALASDAPVGLAKVGLVVNPTDADLDAITGSVPLD
MIQLHGQESVERVAEIKTRYGLPVMKVIGVAEAADLDPIDLYAQVADQLMVDAKAPKGAKLPGGNGISFDWQLLASKKYW
QAPWMLAGGLTPENVAEAIRKTGARQVDVASGVESAPAQKDPDLMRAFVEAAQAV
>Mature_214_residues
ADIRVKICGMKTRADMEAAAAAGAAYVGLNFYAKSARSVTIAQAAALASDAPVGLAKVGLVVNPTDADLDAITGSVPLDM
IQLHGQESVERVAEIKTRYGLPVMKVIGVAEAADLDPIDLYAQVADQLMVDAKAPKGAKLPGGNGISFDWQLLASKKYWQ
APWMLAGGLTPENVAEAIRKTGARQVDVASGVESAPAQKDPDLMRAFVEAAQAV

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=215, Percent_Identity=33.4883720930233, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320210, Length=231, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_SILST (Q1GK79)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612199.1
- ProteinModelPortal:   Q1GK79
- SMR:   Q1GK79
- STRING:   Q1GK79
- GeneID:   4078652
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0204
- NMPDR:   fig|292414.1.peg.2600
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   IQLHGRE
- PhylomeDB:   Q1GK79
- ProtClustDB:   PRK01222
- BioCyc:   SSP292414:TM1040_0204-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 22433; Mature: 22302

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIRVKICGMKTRADMEAAAAAGAAYVGLNFYAKSARSVTIAQAAALASDAPVGLAKVG
CCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHCC
LVVNPTDADLDAITGSVPLDMIQLHGQESVERVAEIKTRYGLPVMKVIGVAEAADLDPID
EEECCCCCCHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
LYAQVADQLMVDAKAPKGAKLPGGNGISFDWQLLASKKYWQAPWMLAGGLTPENVAEAIR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHEEECCCCHHCCCEEEECCCCHHHHHHHHH
KTGARQVDVASGVESAPAQKDPDLMRAFVEAAQAV
HCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADIRVKICGMKTRADMEAAAAAGAAYVGLNFYAKSARSVTIAQAAALASDAPVGLAKVG
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHCC
LVVNPTDADLDAITGSVPLDMIQLHGQESVERVAEIKTRYGLPVMKVIGVAEAADLDPID
EEECCCCCCHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
LYAQVADQLMVDAKAPKGAKLPGGNGISFDWQLLASKKYWQAPWMLAGGLTPENVAEAIR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHEEECCCCHHCCCEEEECCCCHHHHHHHHH
KTGARQVDVASGVESAPAQKDPDLMRAFVEAAQAV
HCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA