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The map label for this gene is aroA

Identifier: 99080040

GI number: 99080040

Start: 216374

End: 217726

Strand: Reverse

Name: aroA

Synonym: TM1040_0199

Alternate gene names: 99080040

Gene position: 217726-216374 (Counterclockwise)

Preceding gene: 99080042

Following gene: 99080039

Centisome position: 6.8

GC content: 63.27

Gene sequence:

>1353_bases
ATGTCCGGCCACGGCACGCCCATCCCGATGACCTCCCGCCGCGCAAGCCCCCTCAAAGGCGAGGCGCATGTCCCTGGCGA
CAAGTCGATTTCGCATCGCTCATTGATCCTTGGCGCAATGGCTGTGGGTGAGACAAAAATCTCCGGCCTCCTTGAGGGCG
AAGATGTGCTCGACACCGCCAAGGCGATGCAGGCTTTTGGGGCCGAGGTCGTCAATCACGGCGGTGGAGAATGGTCCGTC
TTTGGCGTGGGCGTCGGCGGTTTTGCCGAGCCGGAAAACGTGATTGACTGCGGCAATTCCGGCACTGGTGTGCGGCTCAT
CATGGGCGCGATGGCGACCTCGCCGATCACCGCGACCTTTACCGGCGATGCCTCGCTCAACAAACGCCCGATGGCGCGTG
TGACCGATCCGCTTGCGCTCTTTGGCGCGCAATCCGTGGGCCGCGAGGGCGGCCGTCTGCCGATGACCATCGTTGGCGCG
GCCGAGCCCGTGCCGGTGCGCTATGAGGTGCCGGTGCCCTCAGCGCAGGTGAAATCTGCCGTTCTGCTTGCAGGCCTCAA
TGCGCCCGGCAAAACCGTTGTGATTGAGCGCGAAGCCACCCGCGACCATTCCGAGCGGATGCTTGCGGGCTTTGGGGCTG
AAATCACGGTTGAGGACACCAAGGAAGGCCGCGTGATTACCCTCACCGGTCAGCCTGAGCTGAAACCGCAAGTGATTGCA
GTACCGCGCGATCCCTCCTCTGCCGCCTTCCCGGTTTGCGCCGCGCTCATCACGCCCGGTTCTGACGTGCTGGTGCCGGG
GATTGGTCTCAACCCGACCCGCGCGGGCCTGTTCTACACCCTGCAAGACATGGGCGCGGATCTGACGTTTGAGAATCCTC
GGACCGAAGGCGGCGAACCTGTGGCCGATCTGCGCGCCAAATACTCGCCCGACATGAAAGGGATCGAGGTCCCACCAGAA
CGCGCCGCGTCGATGATTGACGAGTATCCCGTTCTGTCTGTGGTGGCCTCTTTTGCCACCGGAACCACCATGATGGCTGG
CGTCAAGGAATTGCGCGTGAAGGAAAGCGACCGCATCGATGCAATGGCAAAGGGCCTGCGCGCCAATGGTGTCACCGTCG
AGGAAGGCGAGGACTGGTGGAGCGTCGAAGGCTGCGGCCCCGAGGGTGTCAAAGGCGGTGGCACTGCCGAGAGCTTCCTT
GATCACCGCATCGCCATGTCGTTCATGGTGATGGGTATGGGCGCACAAAACCCGGTCTCCGTCGACGATGGCAGCCCGAT
CGCGACGTCCTTTCCCATCTTCGAGCGGCTGATGGGCGATCTTGGGGCGTCGATCATCCGCACGGATGGCTGA

Upstream 100 bases:

>100_bases
ACGGTGAACACTCTCCGCCGCGCGCCCATTCTCTTCTGCGTGGACGTGCTGAGGTGAATGCGCTAACAGCTTTTGCGACC
GACTGATGAGAGGAGCCCAC

Downstream 100 bases:

>100_bases
CGTCGATCTTTCCCGCATGCCGCCACCGCAAGGTTTGCGAGTGGTGACAGGTGGCCCCCTTGATCCACAAGCGCTGGCGG
AGCTGCGGGCAGAGGCGATG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSGHGTPIPMTSRRASPLKGEAHVPGDKSISHRSLILGAMAVGETKISGLLEGEDVLDTAKAMQAFGAEVVNHGGGEWSV
FGVGVGGFAEPENVIDCGNSGTGVRLIMGAMATSPITATFTGDASLNKRPMARVTDPLALFGAQSVGREGGRLPMTIVGA
AEPVPVRYEVPVPSAQVKSAVLLAGLNAPGKTVVIEREATRDHSERMLAGFGAEITVEDTKEGRVITLTGQPELKPQVIA
VPRDPSSAAFPVCAALITPGSDVLVPGIGLNPTRAGLFYTLQDMGADLTFENPRTEGGEPVADLRAKYSPDMKGIEVPPE
RAASMIDEYPVLSVVASFATGTTMMAGVKELRVKESDRIDAMAKGLRANGVTVEEGEDWWSVEGCGPEGVKGGGTAESFL
DHRIAMSFMVMGMGAQNPVSVDDGSPIATSFPIFERLMGDLGASIIRTDG

Sequences:

>Translated_450_residues
MSGHGTPIPMTSRRASPLKGEAHVPGDKSISHRSLILGAMAVGETKISGLLEGEDVLDTAKAMQAFGAEVVNHGGGEWSV
FGVGVGGFAEPENVIDCGNSGTGVRLIMGAMATSPITATFTGDASLNKRPMARVTDPLALFGAQSVGREGGRLPMTIVGA
AEPVPVRYEVPVPSAQVKSAVLLAGLNAPGKTVVIEREATRDHSERMLAGFGAEITVEDTKEGRVITLTGQPELKPQVIA
VPRDPSSAAFPVCAALITPGSDVLVPGIGLNPTRAGLFYTLQDMGADLTFENPRTEGGEPVADLRAKYSPDMKGIEVPPE
RAASMIDEYPVLSVVASFATGTTMMAGVKELRVKESDRIDAMAKGLRANGVTVEEGEDWWSVEGCGPEGVKGGGTAESFL
DHRIAMSFMVMGMGAQNPVSVDDGSPIATSFPIFERLMGDLGASIIRTDG
>Mature_449_residues
SGHGTPIPMTSRRASPLKGEAHVPGDKSISHRSLILGAMAVGETKISGLLEGEDVLDTAKAMQAFGAEVVNHGGGEWSVF
GVGVGGFAEPENVIDCGNSGTGVRLIMGAMATSPITATFTGDASLNKRPMARVTDPLALFGAQSVGREGGRLPMTIVGAA
EPVPVRYEVPVPSAQVKSAVLLAGLNAPGKTVVIEREATRDHSERMLAGFGAEITVEDTKEGRVITLTGQPELKPQVIAV
PRDPSSAAFPVCAALITPGSDVLVPGIGLNPTRAGLFYTLQDMGADLTFENPRTEGGEPVADLRAKYSPDMKGIEVPPER
AASMIDEYPVLSVVASFATGTTMMAGVKELRVKESDRIDAMAKGLRANGVTVEEGEDWWSVEGCGPEGVKGGGTAESFLD
HRIAMSFMVMGMGAQNPVSVDDGSPIATSFPIFERLMGDLGASIIRTDG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=439, Percent_Identity=29.1571753986333, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320332, Length=471, Percent_Identity=27.6008492569002, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_SILST (Q1GK84)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612194.1
- ProteinModelPortal:   Q1GK84
- SMR:   Q1GK84
- STRING:   Q1GK84
- GeneID:   4078647
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0199
- NMPDR:   fig|292414.1.peg.2605
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   IEVPPER
- ProtClustDB:   PRK02427
- BioCyc:   SSP292414:TM1040_0199-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46842; Mature: 46711

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHGTPIPMTSRRASPLKGEAHVPGDKSISHRSLILGAMAVGETKISGLLEGEDVLDTA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHCCHHHHHEECCHHHHHHH
KAMQAFGAEVVNHGGGEWSVFGVGVGGFAEPENVIDCGNSGTGVRLIMGAMATSPITATF
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHEEHHHCCCCEEEEE
TGDASLNKRPMARVTDPLALFGAQSVGREGGRLPMTIVGAAEPVPVRYEVPVPSAQVKSA
ECCCCCCCCCHHHHCCHHHHHCCHHHCCCCCCCCEEEEECCCCCCEEEECCCCCHHHCCE
VLLAGLNAPGKTVVIEREATRDHSERMLAGFGAEITVEDTKEGRVITLTGQPELKPQVIA
EEEEECCCCCCEEEEEEHHCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEEE
VPRDPSSAAFPVCAALITPGSDVLVPGIGLNPTRAGLFYTLQDMGADLTFENPRTEGGEP
ECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEHHHCCCCEEECCCCCCCCCC
VADLRAKYSPDMKGIEVPPERAASMIDEYPVLSVVASFATGTTMMAGVKELRVKESDRID
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
AMAKGLRANGVTVEEGEDWWSVEGCGPEGVKGGGTAESFLDHRIAMSFMVMGMGAQNPVS
HHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
VDDGSPIATSFPIFERLMGDLGASIIRTDG
CCCCCCCCCCCHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
SGHGTPIPMTSRRASPLKGEAHVPGDKSISHRSLILGAMAVGETKISGLLEGEDVLDTA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHCCHHHHHEECCHHHHHHH
KAMQAFGAEVVNHGGGEWSVFGVGVGGFAEPENVIDCGNSGTGVRLIMGAMATSPITATF
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHEEHHHCCCCEEEEE
TGDASLNKRPMARVTDPLALFGAQSVGREGGRLPMTIVGAAEPVPVRYEVPVPSAQVKSA
ECCCCCCCCCHHHHCCHHHHHCCHHHCCCCCCCCEEEEECCCCCCEEEECCCCCHHHCCE
VLLAGLNAPGKTVVIEREATRDHSERMLAGFGAEITVEDTKEGRVITLTGQPELKPQVIA
EEEEECCCCCCEEEEEEHHCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEEE
VPRDPSSAAFPVCAALITPGSDVLVPGIGLNPTRAGLFYTLQDMGADLTFENPRTEGGEP
ECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEHHHCCCCEEECCCCCCCCCC
VADLRAKYSPDMKGIEVPPERAASMIDEYPVLSVVASFATGTTMMAGVKELRVKESDRID
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
AMAKGLRANGVTVEEGEDWWSVEGCGPEGVKGGGTAESFLDHRIAMSFMVMGMGAQNPVS
HHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
VDDGSPIATSFPIFERLMGDLGASIIRTDG
CCCCCCCCCCCHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA