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The map label for this gene is prpB [C]

Identifier: 99080004

GI number: 99080004

Start: 179945

End: 180706

Strand: Reverse

Name: prpB [C]

Synonym: TM1040_0163

Alternate gene names: 99080004

Gene position: 180706-179945 (Counterclockwise)

Preceding gene: 99080005

Following gene: 99080000

Centisome position: 5.65

GC content: 59.97

Gene sequence:

>762_bases
ATGTCGCAGATTGAAAAAGCCACGCGCTTTGCCGCGCTCCACCAAAAAGGTGATCCAGTGGTGCTCTACAACATCTGGGA
TGCAGCAGGCGCAAAAGCACTGGCTGAAAACGGTGCGCCTGCGGTCGCGACAGGAAGCTTGTCGATGGCGGCAGCACATG
GCTACGCAGACGGTGAGAGCATACCCCTTGATTTTGTCCTGCGCATCGTGGCGCGGATCTGTGAAACGGTTTCTGTCCCT
GTCACGGTCGATTTTGAAGGCGGCTATGCAACCGCGCCTTCCGAAGTTGGCGAGAACGTTCGCAAGGTCCTGCGTGCGGG
CGCCGTCGGGATCAATTTCGAAGACCGCGTTGTGGGCGGCTCGGGATTGCATCCGGTTTCCGTACAGGTCGACCGCATTC
ACGCCATCAAAACGGTCGCGGCAGAAGAAGGCGTGCCGCTGTTTATCAACGCCCGGACCGATCTGTTCCTTGGACGCGAC
CCCGAGACGCATCGAGACCAGCTGCCAGAGGCGCTCGAGCGAGCAGCCGCCTATTCAGAGGCCGGAGCCTGCGGTTTCTT
TGTGCCGGGGCTTACAGAGCTCGACCTCATCGCGCACTGTGTGGAGCATACGCACCTGCCGGTAAACGTAATGATGCGTG
GGCAGGCCCAGTCCCTCAAAGAAATTGCGGCTTTGAATGTCGCGCGGGTCAGTTACGGTCCGGGACCGTTCTTCAAGTTC
CTGACCGACCTCAAAGCTGCGGAGGCCAGACTGCGCGCCTAG

Upstream 100 bases:

>100_bases
GCGGCGCGATTTTCTGTCTGTGTTATTTCAGGGCACCTGCCATACCGGGCCGCACTGGCCGTACTGAGTGGCGCAGAGCA
TTTGAAGAGAGGATTTACCC

Downstream 100 bases:

>100_bases
AGTTTGCGCATCACCGTGTGGTGTGCAGTCCCGCCGCGCCGCTGGCGCGGCGCCGGGGGCGGGAACCACCCCGGCGCCCT
CAGCTCTCTAGGCTTATCCG

Product: putative carboxyphosphonoenolpyruvate phosphonomutase protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSQIEKATRFAALHQKGDPVVLYNIWDAAGAKALAENGAPAVATGSLSMAAAHGYADGESIPLDFVLRIVARICETVSVP
VTVDFEGGYATAPSEVGENVRKVLRAGAVGINFEDRVVGGSGLHPVSVQVDRIHAIKTVAAEEGVPLFINARTDLFLGRD
PETHRDQLPEALERAAAYSEAGACGFFVPGLTELDLIAHCVEHTHLPVNVMMRGQAQSLKEIAALNVARVSYGPGPFFKF
LTDLKAAEARLRA

Sequences:

>Translated_253_residues
MSQIEKATRFAALHQKGDPVVLYNIWDAAGAKALAENGAPAVATGSLSMAAAHGYADGESIPLDFVLRIVARICETVSVP
VTVDFEGGYATAPSEVGENVRKVLRAGAVGINFEDRVVGGSGLHPVSVQVDRIHAIKTVAAEEGVPLFINARTDLFLGRD
PETHRDQLPEALERAAAYSEAGACGFFVPGLTELDLIAHCVEHTHLPVNVMMRGQAQSLKEIAALNVARVSYGPGPFFKF
LTDLKAAEARLRA
>Mature_252_residues
SQIEKATRFAALHQKGDPVVLYNIWDAAGAKALAENGAPAVATGSLSMAAAHGYADGESIPLDFVLRIVARICETVSVPV
TVDFEGGYATAPSEVGENVRKVLRAGAVGINFEDRVVGGSGLHPVSVQVDRIHAIKTVAAEEGVPLFINARTDLFLGRDP
ETHRDQLPEALERAAAYSEAGACGFFVPGLTELDLIAHCVEHTHLPVNVMMRGQAQSLKEIAALNVARVSYGPGPFFKFL
TDLKAAEARLRA

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=229, Percent_Identity=32.7510917030568, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26883; Mature: 26752

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIEKATRFAALHQKGDPVVLYNIWDAAGAKALAENGAPAVATGSLSMAAAHGYADGES
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCEEEECCCHHHHHCCCCCCCC
IPLDFVLRIVARICETVSVPVTVDFEGGYATAPSEVGENVRKVLRAGAVGINFEDRVVGG
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECC
SGLHPVSVQVDRIHAIKTVAAEEGVPLFINARTDLFLGRDPETHRDQLPEALERAAAYSE
CCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHC
AGACGFFVPGLTELDLIAHCVEHTHLPVNVMMRGQAQSLKEIAALNVARVSYGPGPFFKF
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHH
LTDLKAAEARLRA
HHHHHHHHHHHCC
>Mature Secondary Structure 
SQIEKATRFAALHQKGDPVVLYNIWDAAGAKALAENGAPAVATGSLSMAAAHGYADGES
CHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCEEEECCCHHHHHCCCCCCCC
IPLDFVLRIVARICETVSVPVTVDFEGGYATAPSEVGENVRKVLRAGAVGINFEDRVVGG
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECC
SGLHPVSVQVDRIHAIKTVAAEEGVPLFINARTDLFLGRDPETHRDQLPEALERAAAYSE
CCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHC
AGACGFFVPGLTELDLIAHCVEHTHLPVNVMMRGQAQSLKEIAALNVARVSYGPGPFFKF
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHH
LTDLKAAEARLRA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]