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The map label for this gene is slt [H]

Identifier: 99079990

GI number: 99079990

Start: 163129

End: 165096

Strand: Reverse

Name: slt [H]

Synonym: TM1040_0149

Alternate gene names: 99079990

Gene position: 165096-163129 (Counterclockwise)

Preceding gene: 99079992

Following gene: 99079987

Centisome position: 5.16

GC content: 63.01

Gene sequence:

>1968_bases
ATGACACGCAGACTCGCCTGCTCATTGCTGATTTGCGCGACCCTTTTTGGCGCGCCCGCCCTAGCGCGCGGGCTGGACAC
CGCCCTGCCCCTGATGCGCGACGGACAATGGCAGGAGGCGCTGATCGATGCAGGAAGCGAAGGCTCGATCCAGCGTGACA
TCATCGAGTGGCATCGCCTGCGCGCGGGTGAAGGCACGGCACGCGATGTATTGTCCTTCATCGACCGCCGTCCCGACTGG
CCCGGCATGGACTATCTGCGCCGCCAGAGCGAAGAGACTCTTGCCAAGGCCGGACACTCCGCCATCCTCGCGTTTTATCG
CGATATGCCCTCGGCCCAGACCGCCGAAGGCGCGCTCAGCCTCGGGGAAGCGCTGATCGAGGCCGGGCGCACAGGCGAAG
GCCAGGCAGAAATCGTGCGCGCTTGGCGTAGCATGGCGATGCCGCAGGAGGTCCACGACGCCTATCTAGCGCGACACAAA
ACCCTTTTGGCCGCACACCACGAAGCACGCCTCAGCCGTTTGCTTTGGGACGGGCACAAAGTGTCCTCGCGTCGGATGCT
GGAGCTTGTGAGCGATGGTGCGCGCAAACTGGCGGAAGCGCGCATTGCCTTGCAGGAGCAGGCCAACGGCGTAGACGCGG
CAATTGCTGCAGTGCCGGATGCGCTCAAGGACGATGCAGGGCTGGCCTATGACCGTTTCGCCTGGCGCGACGCAAAGCGA
CGCCAGGATGATGCGATTGCCATGATGTTTGAACGCTCCACCTCTGCCGAGGCGCTGGGGGAGCCGGCAAAATGGCTGCG
CAGACGTGCGGATTTCGCGCGTCAGCTGATGCGCGACAATGACAATACCCGCGCCTATAAGCTCGCGGCCTATCACTTTG
CCACGCCCGATGCTGGCTATGGCTACGCCGACAACGAATGGATTGCGGGCTATGTCGCGTTGCGCAAGCTCGACGATGCG
GAACTTGCCGTCTATCATTTCACGCGTTTCCTTGCCGCGGTTGAAAGCCCGATCTCCGTGGGCCGTGCCGGATATTGGCT
CGGGCGGGCCTATGCGCAGCTCGGTGAGATCGACAAGGCTCACGCGGCCTATCGGCTGGGGGCGAAATATCAAAGCTCCT
ATTACGGGCTGTTCTCTGCCCAGGCGCTTGGGCGCAGCTTCGATCCGCGCCTGACCCGTCCAGAGGCCCCGAACTGGCGC
AACGCCCCATTTTTGCAGTCCTCAGTCTATGCCGCAGGCATTGCCTTGCTGGAGGCCGGTGAGGTGTCGCTTGCAGAGCG
GTTCCTCACTCATCTCGTGGAAAGCCTCCCCCCGGATCAGGCGTTGCAACTGGGGCAGATGGCTGTCGACATGAAACTGC
CGCATCTGGCCGTGATGATCTCCAAGCGAGCTGCTCAGGACGGATTGGAGCTTGCGGGTGCCTATTACCCGCTGCATCCG
GTTACCGACCTCGACCTCCCCATGGCGCCCGAGATGGTTCTGGCGATTGCCCGCCGCGAGAGCGAATTTGACCCTGTGGT
CGTAAGCCATGCGGGCGCGCGCGGGTTGATGCAAGTGATGCCCGGCACCGCAGAACTGGTCGCCAAGGGCCTCGGCATTC
TGGAGGAGCACGCGGTCGCGCGCCTCACCTCGGACTGGCAATATAACGCAAAGCTGGGCGCCACCTACCTTGCGGCGCTG
GCCAAGGAATTCGACGGCAACGTGGTCCTGATGGCGGCGGGCTACAACGCGGGGCCGCACCGTCCGATCGCCTGGATGGA
ACGCTATGGTGATCCCCGTGATCCAAAGGTCGATGTGATCGACTGGATCGAGCACATCCCCTTTAATGAAACCCGCAATT
ACGTCATGCGCGTGACCGAGAGCCTGCCGGTTTATCGAGCGCGGCTTGGAGAAGCGCCTCTGCCCGTGCCCTTCTCACGC
GAACTATCGGGCCGCACGCTTCAGGCTTTCGCGCCAAAAGGTGAATAG

Upstream 100 bases:

>100_bases
CTTGCCCGGACGCGCGCGCTGGCGGGAAATTGAGTTGCACCCCGCGACATTCGCACTATCGTCACAGCCTCTATCCCCGG
ATTGCTGGAAAATGCAAGAG

Downstream 100 bases:

>100_bases
ACCTGCGGCGACGACGATGGCAGCACCGATGATCACATTGGTGCGCATATCCTCGCCAAAGACCAACAGCCCGATCATCG
CAGCCCAAGGGAGCTGCATG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MTRRLACSLLICATLFGAPALARGLDTALPLMRDGQWQEALIDAGSEGSIQRDIIEWHRLRAGEGTARDVLSFIDRRPDW
PGMDYLRRQSEETLAKAGHSAILAFYRDMPSAQTAEGALSLGEALIEAGRTGEGQAEIVRAWRSMAMPQEVHDAYLARHK
TLLAAHHEARLSRLLWDGHKVSSRRMLELVSDGARKLAEARIALQEQANGVDAAIAAVPDALKDDAGLAYDRFAWRDAKR
RQDDAIAMMFERSTSAEALGEPAKWLRRRADFARQLMRDNDNTRAYKLAAYHFATPDAGYGYADNEWIAGYVALRKLDDA
ELAVYHFTRFLAAVESPISVGRAGYWLGRAYAQLGEIDKAHAAYRLGAKYQSSYYGLFSAQALGRSFDPRLTRPEAPNWR
NAPFLQSSVYAAGIALLEAGEVSLAERFLTHLVESLPPDQALQLGQMAVDMKLPHLAVMISKRAAQDGLELAGAYYPLHP
VTDLDLPMAPEMVLAIARRESEFDPVVVSHAGARGLMQVMPGTAELVAKGLGILEEHAVARLTSDWQYNAKLGATYLAAL
AKEFDGNVVLMAAGYNAGPHRPIAWMERYGDPRDPKVDVIDWIEHIPFNETRNYVMRVTESLPVYRARLGEAPLPVPFSR
ELSGRTLQAFAPKGE

Sequences:

>Translated_655_residues
MTRRLACSLLICATLFGAPALARGLDTALPLMRDGQWQEALIDAGSEGSIQRDIIEWHRLRAGEGTARDVLSFIDRRPDW
PGMDYLRRQSEETLAKAGHSAILAFYRDMPSAQTAEGALSLGEALIEAGRTGEGQAEIVRAWRSMAMPQEVHDAYLARHK
TLLAAHHEARLSRLLWDGHKVSSRRMLELVSDGARKLAEARIALQEQANGVDAAIAAVPDALKDDAGLAYDRFAWRDAKR
RQDDAIAMMFERSTSAEALGEPAKWLRRRADFARQLMRDNDNTRAYKLAAYHFATPDAGYGYADNEWIAGYVALRKLDDA
ELAVYHFTRFLAAVESPISVGRAGYWLGRAYAQLGEIDKAHAAYRLGAKYQSSYYGLFSAQALGRSFDPRLTRPEAPNWR
NAPFLQSSVYAAGIALLEAGEVSLAERFLTHLVESLPPDQALQLGQMAVDMKLPHLAVMISKRAAQDGLELAGAYYPLHP
VTDLDLPMAPEMVLAIARRESEFDPVVVSHAGARGLMQVMPGTAELVAKGLGILEEHAVARLTSDWQYNAKLGATYLAAL
AKEFDGNVVLMAAGYNAGPHRPIAWMERYGDPRDPKVDVIDWIEHIPFNETRNYVMRVTESLPVYRARLGEAPLPVPFSR
ELSGRTLQAFAPKGE
>Mature_654_residues
TRRLACSLLICATLFGAPALARGLDTALPLMRDGQWQEALIDAGSEGSIQRDIIEWHRLRAGEGTARDVLSFIDRRPDWP
GMDYLRRQSEETLAKAGHSAILAFYRDMPSAQTAEGALSLGEALIEAGRTGEGQAEIVRAWRSMAMPQEVHDAYLARHKT
LLAAHHEARLSRLLWDGHKVSSRRMLELVSDGARKLAEARIALQEQANGVDAAIAAVPDALKDDAGLAYDRFAWRDAKRR
QDDAIAMMFERSTSAEALGEPAKWLRRRADFARQLMRDNDNTRAYKLAAYHFATPDAGYGYADNEWIAGYVALRKLDDAE
LAVYHFTRFLAAVESPISVGRAGYWLGRAYAQLGEIDKAHAAYRLGAKYQSSYYGLFSAQALGRSFDPRLTRPEAPNWRN
APFLQSSVYAAGIALLEAGEVSLAERFLTHLVESLPPDQALQLGQMAVDMKLPHLAVMISKRAAQDGLELAGAYYPLHPV
TDLDLPMAPEMVLAIARRESEFDPVVVSHAGARGLMQVMPGTAELVAKGLGILEEHAVARLTSDWQYNAKLGATYLAALA
KEFDGNVVLMAAGYNAGPHRPIAWMERYGDPRDPKVDVIDWIEHIPFNETRNYVMRVTESLPVYRARLGEAPLPVPFSRE
LSGRTLQAFAPKGE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=266, Percent_Identity=29.3233082706767, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72448; Mature: 72317

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRLACSLLICATLFGAPALARGLDTALPLMRDGQWQEALIDAGSEGSIQRDIIEWHRL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHH
RAGEGTARDVLSFIDRRPDWPGMDYLRRQSEETLAKAGHSAILAFYRDMPSAQTAEGALS
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
LGEALIEAGRTGEGQAEIVRAWRSMAMPQEVHDAYLARHKTLLAAHHEARLSRLLWDGHK
HHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VSSRRMLELVSDGARKLAEARIALQEQANGVDAAIAAVPDALKDDAGLAYDRFAWRDAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH
RQDDAIAMMFERSTSAEALGEPAKWLRRRADFARQLMRDNDNTRAYKLAAYHFATPDAGY
CCCCHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCC
GYADNEWIAGYVALRKLDDAELAVYHFTRFLAAVESPISVGRAGYWLGRAYAQLGEIDKA
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCHHHH
HAAYRLGAKYQSSYYGLFSAQALGRSFDPRLTRPEAPNWRNAPFLQSSVYAAGIALLEAG
HHHHHHCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
EVSLAERFLTHLVESLPPDQALQLGQMAVDMKLPHLAVMISKRAAQDGLELAGAYYPLHP
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCC
VTDLDLPMAPEMVLAIARRESEFDPVVVSHAGARGLMQVMPGTAELVAKGLGILEEHAVA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RLTSDWQYNAKLGATYLAALAKEFDGNVVLMAAGYNAGPHRPIAWMERYGDPRDPKVDVI
HHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCHHH
DWIEHIPFNETRNYVMRVTESLPVYRARLGEAPLPVPFSRELSGRTLQAFAPKGE
HHHHHCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
TRRLACSLLICATLFGAPALARGLDTALPLMRDGQWQEALIDAGSEGSIQRDIIEWHRL
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHH
RAGEGTARDVLSFIDRRPDWPGMDYLRRQSEETLAKAGHSAILAFYRDMPSAQTAEGALS
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
LGEALIEAGRTGEGQAEIVRAWRSMAMPQEVHDAYLARHKTLLAAHHEARLSRLLWDGHK
HHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VSSRRMLELVSDGARKLAEARIALQEQANGVDAAIAAVPDALKDDAGLAYDRFAWRDAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH
RQDDAIAMMFERSTSAEALGEPAKWLRRRADFARQLMRDNDNTRAYKLAAYHFATPDAGY
CCCCHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCC
GYADNEWIAGYVALRKLDDAELAVYHFTRFLAAVESPISVGRAGYWLGRAYAQLGEIDKA
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCHHHH
HAAYRLGAKYQSSYYGLFSAQALGRSFDPRLTRPEAPNWRNAPFLQSSVYAAGIALLEAG
HHHHHHCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
EVSLAERFLTHLVESLPPDQALQLGQMAVDMKLPHLAVMISKRAAQDGLELAGAYYPLHP
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCC
VTDLDLPMAPEMVLAIARRESEFDPVVVSHAGARGLMQVMPGTAELVAKGLGILEEHAVA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RLTSDWQYNAKLGATYLAALAKEFDGNVVLMAAGYNAGPHRPIAWMERYGDPRDPKVDVI
HHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCHHH
DWIEHIPFNETRNYVMRVTESLPVYRARLGEAPLPVPFSRELSGRTLQAFAPKGE
HHHHHCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]