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The map label for this gene is pnp

Identifier: 99079919

GI number: 99079919

Start: 81035

End: 83170

Strand: Reverse

Name: pnp

Synonym: TM1040_0078

Alternate gene names: 99079919

Gene position: 83170-81035 (Counterclockwise)

Preceding gene: 99079920

Following gene: 99079918

Centisome position: 2.6

GC content: 59.46

Gene sequence:

>2136_bases
ATGTTCAACGTAACGACGAAAACAATGCAGTGGGGCGAAGAAACGCTCACACTGGAAACCGGCAAGGTTGCGCGCCAGGC
AGATGGCACCGTGATCGCGACGCTCGGCGAAACCTCGGTCATGGCCAACGTGACCTACGCCCGCGAACAAAAGCCCGGTC
AGGATTTCTTTCCGCTGACCGTTCACTACCAGGAAAAATACTACGCCGCCGGTAAGATTCCGGGCGGCTTTTTCAAACGT
GAAGCGCGTCCGACTGAAAAAGAGACGCTGACTGCACGTCTGATCGACCGCCCGATCCGCCCGCTGTTCGTTCCCGGCTT
CAAAAACGAAGTTCTGGTGATGTGCACCGTGCTGTCCCACGATCTGGTCAATGACCCCGACATCGTCGCGATGATCGCCG
CCTCCGCCGCGCTGACCATCTCTGGCGTGCCGTTCATGGGTCCGATCGCGGGTGCGCGCGTCGGTTACGAGGATGGCGAG
TACATCCTGAACCCGACCGTCGACGACATGCAGGACCTGCGCCTCAACCCCGAGCAGCGTCTGGACCTTGTTGTTGCAGG
CACCAAAGATGCCGTGATGATGGTCGAATCCGAAGCTTACGAGCTAACCGAAGAAGAAATGCTCGGCGCTGTGACCTTCG
CACACGAGCAAATCCAGCCGGTCATCGATCTGATCGTCTCGCTCGCAGAAGAAACCGCAAAAGAGCCCTTCGACTTCACG
CCGCCGGATTACTCCGACCTCTATGCTGCCGTTAAAGCAGCGGGCGAAGAGAAGATGCGCGCAGCCTATGCGATCTCTGA
CAAGCAGGAACGTGTTGCCGCCGTTGGCGCCGCCAAAGAAGAGATCATCGCGTCTCTGACCGAAGAGCAGCAGGAAGACG
GCAACCTCGGCTCCGCGCTGAAAAAGCTCGAATCTGTTGTTCTGCGGGGCGACGTTGTGAAAAACGGTCGCCGCATCGAC
GGTCGTGCCCTGGATCAGGTCCGCGCGATCGAGTGCCAGACCGGTCTTTTGCCGCGTACACACGGGTCTGCGCTGTTCAC
CCGTGGTGAAACGCAGGGTCTCGTGGTGACCACGCTCGGCACCGGCGACGATGAGCAGTTCATCGACGCGCTGCACGGCA
ACTTCAAATCGAACTTCCTGCTGCACTACAACTTCCCGCCCTACTCGGTTGGTGAAGTGGGCCGCGTGGGCTCTCCGGGT
CGTCGTGAAATCGGCCACGGCAAGCTCGCATGGCGCGCGCTGCAGGCGGTTCTGCCGGCTCCGACCGACTTCCCCTACAC
CATCCGCGTGGTCTCTGAGATCACCGAATCGAACGGCTCTTCCTCCATGGCTTCGGTCTGTGGTGGCTCGCTCTCGATGA
TGGACGCAGGTGTGCCGCTGAAAGCACCGGTTGCCGGTGTCGCCATGGGCCTCGTGCTCGAAGAGGACGGCTCCTACGGC
ATCCTGACCGATATTCTTGGCGACGAGGATCACCTCGGCGACATGGACTTTAAGGTTGCGGGTACCGAAGCAGGCATCAC
CTCCTTGCAGATGGACATCAAGGTTGCAGGCATCACGCCTGAAATCATGAAAAACGCGCTGGCTCAGGCCAAAGCGGGCC
GCCTGCACATCCTTGGTGAGATGGCCAAATCGCTGACCGAAGCCTCCGAGTTCTCGCAGCACGCACCGCGCATCGAGACC
ATGCAGGTTCCGACCGACAAGATCCGTGAAGTGATCGGTTCCGGCGGTAAGGTCATCCGCGAGATCGTCGAAGTCTCCGG
TGCCAAGGTCGACATCAACGACGACGGCGTGATCAAGATTGCCTCCGCCAACGGCGAAGCGATCCAGAAGGCCTATGACA
TGATCCACGCGATCGTGGCAGAGCCGGAAGAAGGCGCGGTCTACACCGGTAAAGTTGTGAAGATCGTCGACTTCGGCGCC
TTCGTGAACTTCTTCGGCAAGCGCGACGGTCTGGTGCATGTGTCCCAGATCGAAAACCGCCGCCTGAACCACCCCAGCGA
CGTGCTGAAGGAAGGTCAGGAAGTGAAAGTGAAGCTCCTGGGCTTTGACGATCGCGGCAAGGTACGCCTGTCCATGAAAG
TCGTGGATCAGGAAACTGGCGAAGAGATCGTCGCAGAGAAGAAAGAAGCAGAGTAA

Upstream 100 bases:

>100_bases
CGCGAAAGAAAGATAGCATAAGGGTCGGCGGCAATGGGGCCGCCACGCAATTAGGAGACCCGGGATACGCCTGGGAGCGC
TCCGACTTAGGATACGATAG

Downstream 100 bases:

>100_bases
GATCTGTCCCACGACAATCTGATCTGACATAAGGTGCCCGGGAGCGATGTCTCCCGGGCATTTTTTATGGGCCGCGCTCT
GCAAGCGCCGGAAAGGATTT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MFNVTTKTMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTYAREQKPGQDFFPLTVHYQEKYYAAGKIPGGFFKR
EARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSHDLVNDPDIVAMIAASAALTISGVPFMGPIAGARVGYEDGE
YILNPTVDDMQDLRLNPEQRLDLVVAGTKDAVMMVESEAYELTEEEMLGAVTFAHEQIQPVIDLIVSLAEETAKEPFDFT
PPDYSDLYAAVKAAGEEKMRAAYAISDKQERVAAVGAAKEEIIASLTEEQQEDGNLGSALKKLESVVLRGDVVKNGRRID
GRALDQVRAIECQTGLLPRTHGSALFTRGETQGLVVTTLGTGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPG
RREIGHGKLAWRALQAVLPAPTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLVLEEDGSYG
ILTDILGDEDHLGDMDFKVAGTEAGITSLQMDIKVAGITPEIMKNALAQAKAGRLHILGEMAKSLTEASEFSQHAPRIET
MQVPTDKIREVIGSGGKVIREIVEVSGAKVDINDDGVIKIASANGEAIQKAYDMIHAIVAEPEEGAVYTGKVVKIVDFGA
FVNFFGKRDGLVHVSQIENRRLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKVVDQETGEEIVAEKKEAE

Sequences:

>Translated_711_residues
MFNVTTKTMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTYAREQKPGQDFFPLTVHYQEKYYAAGKIPGGFFKR
EARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSHDLVNDPDIVAMIAASAALTISGVPFMGPIAGARVGYEDGE
YILNPTVDDMQDLRLNPEQRLDLVVAGTKDAVMMVESEAYELTEEEMLGAVTFAHEQIQPVIDLIVSLAEETAKEPFDFT
PPDYSDLYAAVKAAGEEKMRAAYAISDKQERVAAVGAAKEEIIASLTEEQQEDGNLGSALKKLESVVLRGDVVKNGRRID
GRALDQVRAIECQTGLLPRTHGSALFTRGETQGLVVTTLGTGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPG
RREIGHGKLAWRALQAVLPAPTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLVLEEDGSYG
ILTDILGDEDHLGDMDFKVAGTEAGITSLQMDIKVAGITPEIMKNALAQAKAGRLHILGEMAKSLTEASEFSQHAPRIET
MQVPTDKIREVIGSGGKVIREIVEVSGAKVDINDDGVIKIASANGEAIQKAYDMIHAIVAEPEEGAVYTGKVVKIVDFGA
FVNFFGKRDGLVHVSQIENRRLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKVVDQETGEEIVAEKKEAE
>Mature_711_residues
MFNVTTKTMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTYAREQKPGQDFFPLTVHYQEKYYAAGKIPGGFFKR
EARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSHDLVNDPDIVAMIAASAALTISGVPFMGPIAGARVGYEDGE
YILNPTVDDMQDLRLNPEQRLDLVVAGTKDAVMMVESEAYELTEEEMLGAVTFAHEQIQPVIDLIVSLAEETAKEPFDFT
PPDYSDLYAAVKAAGEEKMRAAYAISDKQERVAAVGAAKEEIIASLTEEQQEDGNLGSALKKLESVVLRGDVVKNGRRID
GRALDQVRAIECQTGLLPRTHGSALFTRGETQGLVVTTLGTGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPG
RREIGHGKLAWRALQAVLPAPTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLVLEEDGSYG
ILTDILGDEDHLGDMDFKVAGTEAGITSLQMDIKVAGITPEIMKNALAQAKAGRLHILGEMAKSLTEASEFSQHAPRIET
MQVPTDKIREVIGSGGKVIREIVEVSGAKVDINDDGVIKIASANGEAIQKAYDMIHAIVAEPEEGAVYTGKVVKIVDFGA
FVNFFGKRDGLVHVSQIENRRLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKVVDQETGEEIVAEKKEAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=38.4078212290503, Blast_Score=451, Evalue=1e-126,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=45.6521739130435, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=56.8149210903874, Blast_Score=765, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=708, Percent_Identity=33.8983050847458, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17535281, Length=84, Percent_Identity=48.8095238095238, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=107, Percent_Identity=40.1869158878505, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=37.9407616361072, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=37.9407616361072, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=37.9407616361072, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=37.8048780487805, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=42.8571428571429, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_SILST (Q1GKK5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612073.1
- ProteinModelPortal:   Q1GKK5
- SMR:   Q1GKK5
- STRING:   Q1GKK5
- GeneID:   4075975
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0078
- NMPDR:   fig|292414.1.peg.1130
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q1GKK5
- ProtClustDB:   PRK11824
- BioCyc:   SSP292414:TM1040_0078-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76972; Mature: 76972

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNVTTKTMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTYAREQKPGQDFFPLT
CCCCCCHHHHCCCEEEEEECCCHHHHCCCCEEEEECCCEEEEEEEEHHCCCCCCCCCEEE
VHYQEKYYAAGKIPGGFFKREARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSH
EEECCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEHHHH
DLVNDPDIVAMIAASAALTISGVPFMGPIAGARVGYEDGEYILNPTVDDMQDLRLNPEQR
CCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHC
LDLVVAGTKDAVMMVESEAYELTEEEMLGAVTFAHEQIQPVIDLIVSLAEETAKEPFDFT
EEEEEECCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPDYSDLYAAVKAAGEEKMRAAYAISDKQERVAAVGAAKEEIIASLTEEQQEDGNLGSAL
CCCHHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
KKLESVVLRGDVVKNGRRIDGRALDQVRAIECQTGLLPRTHGSALFTRGETQGLVVTTLG
HHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEC
TGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPGRREIGHGKLAWRALQAVLPA
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLVLEEDGSYG
CCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEHCCCCCCCCCHHHHEEEEEEECCCCEE
ILTDILGDEDHLGDMDFKVAGTEAGITSLQMDIKVAGITPEIMKNALAQAKAGRLHILGE
EEHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCEEHHHH
MAKSLTEASEFSQHAPRIETMQVPTDKIREVIGSGGKVIREIVEVSGAKVDINDDGVIKI
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASANGEAIQKAYDMIHAIVAEPEEGAVYTGKVVKIVDFGAFVNFFGKRDGLVHVSQIENR
ECCCCHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEEHHHHHHHHCCCCCEEEEEHHHCC
RLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKVVDQETGEEIVAEKKEAE
CCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MFNVTTKTMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTYAREQKPGQDFFPLT
CCCCCCHHHHCCCEEEEEECCCHHHHCCCCEEEEECCCEEEEEEEEHHCCCCCCCCCEEE
VHYQEKYYAAGKIPGGFFKREARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSH
EEECCCEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEHHHH
DLVNDPDIVAMIAASAALTISGVPFMGPIAGARVGYEDGEYILNPTVDDMQDLRLNPEQR
CCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHC
LDLVVAGTKDAVMMVESEAYELTEEEMLGAVTFAHEQIQPVIDLIVSLAEETAKEPFDFT
EEEEEECCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPDYSDLYAAVKAAGEEKMRAAYAISDKQERVAAVGAAKEEIIASLTEEQQEDGNLGSAL
CCCHHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
KKLESVVLRGDVVKNGRRIDGRALDQVRAIECQTGLLPRTHGSALFTRGETQGLVVTTLG
HHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEC
TGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPGRREIGHGKLAWRALQAVLPA
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLVLEEDGSYG
CCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEHCCCCCCCCCHHHHEEEEEEECCCCEE
ILTDILGDEDHLGDMDFKVAGTEAGITSLQMDIKVAGITPEIMKNALAQAKAGRLHILGE
EEHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCEEHHHH
MAKSLTEASEFSQHAPRIETMQVPTDKIREVIGSGGKVIREIVEVSGAKVDINDDGVIKI
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASANGEAIQKAYDMIHAIVAEPEEGAVYTGKVVKIVDFGAFVNFFGKRDGLVHVSQIENR
ECCCCHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEEHHHHHHHHCCCCCEEEEEHHHCC
RLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKVVDQETGEEIVAEKKEAE
CCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA