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The map label for this gene is def [H]

Identifier: 94986391

GI number: 94986391

Start: 2413015

End: 2413665

Strand: Reverse

Name: def [H]

Synonym: Dgeo_2292

Alternate gene names: 94986391

Gene position: 2413665-2413015 (Counterclockwise)

Preceding gene: 94986394

Following gene: 94986390

Centisome position: 97.83

GC content: 66.82

Gene sequence:

>651_bases
ATGGCCGAGGCCCCCCGCGTTTACCCCATCCGCCTGTACGGCGACCCGGTGCTGCGCCGCAAGGCGAGGCCCCTGCAAGC
TACCGATACCCTGACCGTGCCCGGCTTTGGCCCACAGTCGGTGCGTGAGGTTGCCAACACGATGCTCGAAACGATGTTCG
AGGCGCGCGGCGTTGGCCTGGCCGCACCCCAGGTGGGTCTGCCTGTCCGCATGTTTGTCGCCGTGGAGTACGAGGATGAC
GAGGAGGAACAGGAAGGCCGGGACGTGCCCCTCAAGTCCCGCGTGCTGCGCGAGTTCGTGATGCTGAATCCGGTCCTGAC
GGTCATCGACAAGAAAAAAGACCGCTCGTACCAGGAAGGCTGCCTCAGCATCCCCGGCATCTACGAGGAAGGTGTGGCCC
GTGCCCGCGCGGTGCAGGTCCGGTACACCGACCTCGACGGCCAGGAACGCACGCTGGAGGCGGACGACTACCTGGCCCGC
GTCTTCCAGCACGAGACGGATCACCTCGACGGCGTGTTTTTCCTCGATCGCCTGCCCCCCGAGGTGACCGAGGACCACCG
CAAGGAGCTGGCCGCCATGCAGCGCCAGGCGAAGGCGTACCTCCAAGGGTTGGCCGAGGCCGAGAAGCGGCGGCGGGAGC
AGGAAGGTTGA

Upstream 100 bases:

>100_bases
CTCGACGAACTCACCCGCCGGTGAGGGAGCTGCCAGTAAGGGAGCTGAAGGTGTCCCGGGGCCTGCGCTTTTGCGGGGGC
CTCTGATAAAACGGGGCGAT

Downstream 100 bases:

>100_bases
GCGCCCCCCGCGTCGCCTTTTTCGGCTCGCCCGCCTTCGCGCTGCCGGTGCTGGAAGCCATCCGCGAGCATTTTGAGGTG
GTGCTGGTCGTCACGCAGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MAEAPRVYPIRLYGDPVLRRKARPLQATDTLTVPGFGPQSVREVANTMLETMFEARGVGLAAPQVGLPVRMFVAVEYEDD
EEEQEGRDVPLKSRVLREFVMLNPVLTVIDKKKDRSYQEGCLSIPGIYEEGVARARAVQVRYTDLDGQERTLEADDYLAR
VFQHETDHLDGVFFLDRLPPEVTEDHRKELAAMQRQAKAYLQGLAEAEKRRREQEG

Sequences:

>Translated_216_residues
MAEAPRVYPIRLYGDPVLRRKARPLQATDTLTVPGFGPQSVREVANTMLETMFEARGVGLAAPQVGLPVRMFVAVEYEDD
EEEQEGRDVPLKSRVLREFVMLNPVLTVIDKKKDRSYQEGCLSIPGIYEEGVARARAVQVRYTDLDGQERTLEADDYLAR
VFQHETDHLDGVFFLDRLPPEVTEDHRKELAAMQRQAKAYLQGLAEAEKRRREQEG
>Mature_215_residues
AEAPRVYPIRLYGDPVLRRKARPLQATDTLTVPGFGPQSVREVANTMLETMFEARGVGLAAPQVGLPVRMFVAVEYEDDE
EEQEGRDVPLKSRVLREFVMLNPVLTVIDKKKDRSYQEGCLSIPGIYEEGVARARAVQVRYTDLDGQERTLEADDYLARV
FQHETDHLDGVFFLDRLPPEVTEDHRKELAAMQRQAKAYLQGLAEAEKRRREQEG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=171, Percent_Identity=33.9181286549708, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789682, Length=166, Percent_Identity=34.9397590361446, Blast_Score=84, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=31.7365269461078, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=30.952380952381, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 24617; Mature: 24486

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAPRVYPIRLYGDPVLRRKARPLQATDTLTVPGFGPQSVREVANTMLETMFEARGVGL
CCCCCCEEEEEECCCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
AAPQVGLPVRMFVAVEYEDDEEEQEGRDVPLKSRVLREFVMLNPVLTVIDKKKDRSYQEG
CCCCCCCCEEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
CLSIPGIYEEGVARARAVQVRYTDLDGQERTLEADDYLARVFQHETDHLDGVFFLDRLPP
CCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCC
EVTEDHRKELAAMQRQAKAYLQGLAEAEKRRREQEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEAPRVYPIRLYGDPVLRRKARPLQATDTLTVPGFGPQSVREVANTMLETMFEARGVGL
CCCCCEEEEEECCCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
AAPQVGLPVRMFVAVEYEDDEEEQEGRDVPLKSRVLREFVMLNPVLTVIDKKKDRSYQEG
CCCCCCCCEEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
CLSIPGIYEEGVARARAVQVRYTDLDGQERTLEADDYLARVFQHETDHLDGVFFLDRLPP
CCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCC
EVTEDHRKELAAMQRQAKAYLQGLAEAEKRRREQEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]