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The map label for this gene is aph [H]

Identifier: 94986355

GI number: 94986355

Start: 2382980

End: 2383759

Strand: Reverse

Name: aph [H]

Synonym: Dgeo_2256

Alternate gene names: 94986355

Gene position: 2383759-2382980 (Counterclockwise)

Preceding gene: 94986359

Following gene: 94986354

Centisome position: 96.62

GC content: 67.05

Gene sequence:

>780_bases
ATGACCGCCGAGCGTCTGAGCCTGCCCGAACCCCTGCGCCGCGTGTTGCCCGCTGCTCGCTGGGAGCCGGTGACCGAGGG
ATGCAGCGGCGCCGGCGTGTGGCGCAGCCAGAACTATGTGGTGAAGGTGCAGGGGCGAGGTGGCCTCCCTGTCAGCACCC
TGCTTCAGGAGCGGGAGCGGCTGCGCTGGTTGCGGGGCCGCGTGCCGGTACCCGTGGTGGTGGGTTACGAGGTCACGCCC
ACCCATGAATATCTGGCGCTCACTCGTCTGCCCGGGATCCCGATGAGCCACCCGGACGCCACGCTCCACCCAGAGCGCCT
CACCGACCTGTTGGCCCGCGCCCTACGCGAACTCCATGCCCTGCCTATCCGTGAGTGCCCGTTTAACCTGTCGCTTCCCG
TCACCCTGCGCCTGGCCCGCGAGCGGGTGCGGGCCGGAGTGGTGGATGAGGCCGACTTTGACGCCGAACGCCAGGGATGG
CGTGCTGCTGACGTTCTCGCCGAACTTCTCCGGACCCGGCCGGAACACGAGGATATCGTCGTCACGCACGGAGACGCCTG
TCTGCCGAACGTCATCCTGAATGGTGAGTATGTGGAAGGCTTCGTCGATGTGGGACGTGCGGGCTTGGCTGACCGCCACG
CGGACCTCGCGCTGGCCTGGCGTAGCCTCACGCGCAATCTGGGGCCGGAATACGCAGAACGCTTCCTCGACCTTTACGGG
CGGGCACTCGTGGACCAGGGCAAGCTGGCGTATTACCAACTGCTGGACGAGTTGTTCTGA

Upstream 100 bases:

>100_bases
TCCAGCAGACCGACCTTCACGCGGGCAGTCTACAAGGGAAGCATGGACGATGAGGGAGGAGCCTCCTGGGTGGCCCCGGC
CCGAGCCTTATCCTGCCGCT

Downstream 100 bases:

>100_bases
TTCTCAGGGAGCTACAGCGGGGCAAATGGCGAGCGGTGGGGCGGCCCGCACACCTCAGACCAGGGACAGTCCGGCCAGAC
CAGGAACAGTCCGGGGTGGT

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: APH(3')V; Kanamycin kinase, type V; Neomycin-kanamycin phosphotransferase type V [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTAERLSLPEPLRRVLPAARWEPVTEGCSGAGVWRSQNYVVKVQGRGGLPVSTLLQERERLRWLRGRVPVPVVVGYEVTP
THEYLALTRLPGIPMSHPDATLHPERLTDLLARALRELHALPIRECPFNLSLPVTLRLARERVRAGVVDEADFDAERQGW
RAADVLAELLRTRPEHEDIVVTHGDACLPNVILNGEYVEGFVDVGRAGLADRHADLALAWRSLTRNLGPEYAERFLDLYG
RALVDQGKLAYYQLLDELF

Sequences:

>Translated_259_residues
MTAERLSLPEPLRRVLPAARWEPVTEGCSGAGVWRSQNYVVKVQGRGGLPVSTLLQERERLRWLRGRVPVPVVVGYEVTP
THEYLALTRLPGIPMSHPDATLHPERLTDLLARALRELHALPIRECPFNLSLPVTLRLARERVRAGVVDEADFDAERQGW
RAADVLAELLRTRPEHEDIVVTHGDACLPNVILNGEYVEGFVDVGRAGLADRHADLALAWRSLTRNLGPEYAERFLDLYG
RALVDQGKLAYYQLLDELF
>Mature_258_residues
TAERLSLPEPLRRVLPAARWEPVTEGCSGAGVWRSQNYVVKVQGRGGLPVSTLLQERERLRWLRGRVPVPVVVGYEVTPT
HEYLALTRLPGIPMSHPDATLHPERLTDLLARALRELHALPIRECPFNLSLPVTLRLARERVRAGVVDEADFDAERQGWR
AADVLAELLRTRPEHEDIVVTHGDACLPNVILNGEYVEGFVDVGRAGLADRHADLALAWRSLTRNLGPEYAERFLDLYGR
ALVDQGKLAYYQLLDELF

Specific function: Resistance to kanamycin and structurally-related aminoglycosides, including amikacin [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 29145; Mature: 29014

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAERLSLPEPLRRVLPAARWEPVTEGCSGAGVWRSQNYVVKVQGRGGLPVSTLLQERER
CCCCCCCCHHHHHHHCCHHCCCCHHCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHH
LRWLRGRVPVPVVVGYEVTPTHEYLALTRLPGIPMSHPDATLHPERLTDLLARALRELHA
HHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LPIRECPFNLSLPVTLRLARERVRAGVVDEADFDAERQGWRAADVLAELLRTRPEHEDIV
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEE
VTHGDACLPNVILNGEYVEGFVDVGRAGLADRHADLALAWRSLTRNLGPEYAERFLDLYG
EECCCCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RALVDQGKLAYYQLLDELF
HHHHCCCCHHHHHHHHHHC
>Mature Secondary Structure 
TAERLSLPEPLRRVLPAARWEPVTEGCSGAGVWRSQNYVVKVQGRGGLPVSTLLQERER
CCCCCCCHHHHHHHCCHHCCCCHHCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHH
LRWLRGRVPVPVVVGYEVTPTHEYLALTRLPGIPMSHPDATLHPERLTDLLARALRELHA
HHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LPIRECPFNLSLPVTLRLARERVRAGVVDEADFDAERQGWRAADVLAELLRTRPEHEDIV
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEE
VTHGDACLPNVILNGEYVEGFVDVGRAGLADRHADLALAWRSLTRNLGPEYAERFLDLYG
EECCCCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RALVDQGKLAYYQLLDELF
HHHHCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6310563; 2987648 [H]