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The map label for this gene is irlS [H]

Identifier: 94986313

GI number: 94986313

Start: 2333683

End: 2335125

Strand: Reverse

Name: irlS [H]

Synonym: Dgeo_2214

Alternate gene names: 94986313

Gene position: 2335125-2333683 (Counterclockwise)

Preceding gene: 94986314

Following gene: 94986312

Centisome position: 94.65

GC content: 73.11

Gene sequence:

>1443_bases
ATGAGGCCACGGGGGATTCGTCTGGCCTTCCCGCTGACCCTGCGTGCTCAGCTCGCCTGGTGGGCTGCCTTGGCGACAGC
GTTGGCGGTGGCGCTGGTGGCCGCTGGGTTGTTTGTTGCGGTGAATAGCTTTTTGTTCGCCGCGCAGCGGGACCGGCTCG
CGGCAGCAGTAGGAGCGGTGCAGGCGCGGGTGGAGACGGCGCTGGGCAGAGGGGATGACCTGCTGGCGGCGCTGCTGGGC
GTGCAGGGAACACTGGCCCCGGCAGACCTGGAAGCGATCCTGGACGCCGATCCGCAGACCCGTCATCTGGACGTGCGGCT
GATCACGGTGGGGAGCGGACAGGTGCGGGTGCTGCCCACACCGCGTTTTCCTGACGGCATCCCGCTCAACCTGCCCCCCG
GCACCTACCGCGAAGGGGACCGTCTGGTGGCGGTGCGTGTGCTGGCGGGGGGACGTGCCGCCTTGACCGTTGTGAGCGAC
GCGCGGGCGCTGGGGGAGGCGCAGGCGGCCTTTGGGCGGGCCTTGTGGGGGCTGGTGCCGCTGGCGCTGCTGCTGTCGCT
GGCGCTGGGGTGGACGGTGGCGGGTCGCCTGCTGGCGCCTGTCCGGACGCTGGAGATGGCCGCGCGTGAGATCGGCGCGA
GTGGCAACCTGCGGCGTCCTGTACCGGGAGCAGGCGAGCGAGACGAACTCGCCCGATTGGCCCTGACCTTACAGGAGACA
TTCATCCGGCTGGCCGACGCCCGTGACCGCCAGCAGGACTTTCTGCGGGCAGCGGCCCATGACCTGCGCAGCCCCCTCGC
GGCCCTGACTGCTCGGGTGGACGCCACGCTCGCCCGTGACCGCGCTCCCGAGCGTTACCGCGCCGAACTGCGCGAGATCG
GCACCGACCTCACCCGGCTGTCCAGCCTGGCGAACCACCTGCTGCTGCTGGCCCGCGACCCGGCGGCCCTCGCCCGGGAG
GCCGTGCCCCTGCGCGACCTGGCGGCGGACGCGGTGGACCGCGCGCGCGAACTCGCCCCCGAGGCGGATGTGGACCTGAT
CGCGCCGCAGCCGGTCGGCGTGGCGGGGGACCGGGTGCTGCTGGGGCAGGCGATCTGGAACCTGACGGTGAACGCGGTCC
TCCACGCGCCCGGCGCCACCGTCACCGTGACGGTGAGGGATGACGGTGAGGGCGGCGGCGTGGTGGAGGTGCGCGACGAC
GGCCCCGGCGTGGACGCTGCCGTGCTGGCGCGGCTGGGGGAAGCCTTCTACCGCCCGGATGCGAGCCGCACAGCCTCCGG
GGGTGCCCGGGGGCACGGCCTGGGCCTCGCACTGGTGCGGCGAGCAGCGGAACTGCACGGCGGCACGCTGCGGTTGGAGA
GTGCGCCGGGGGCGGGCTTCTGCGCCACGTTGCACCTGCCGGCACGGTCCTCCACGGGCGAAGTGCTACGCTCTCCCGCA
TGA

Upstream 100 bases:

>100_bases
GTGAGGCCCGCATCGTAGACGTCTACGTGCGGTATCTGCGCCGCAAGCTGGCGCCCGAGGCGGTGAGCAGCGAGCGGGGG
CGGGGGTACCGGGTGGAGCG

Downstream 100 bases:

>100_bases
CAAAACCTCCAGCCGGGCAGTGGAGCGCGGTTGTGCTGGGCGGCGGCGACCCCGGCGATCCCTTTGCGGCGGCCCATGGC
GTCTCCGTCAAGGCCCTGAT

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MRPRGIRLAFPLTLRAQLAWWAALATALAVALVAAGLFVAVNSFLFAAQRDRLAAAVGAVQARVETALGRGDDLLAALLG
VQGTLAPADLEAILDADPQTRHLDVRLITVGSGQVRVLPTPRFPDGIPLNLPPGTYREGDRLVAVRVLAGGRAALTVVSD
ARALGEAQAAFGRALWGLVPLALLLSLALGWTVAGRLLAPVRTLEMAAREIGASGNLRRPVPGAGERDELARLALTLQET
FIRLADARDRQQDFLRAAAHDLRSPLAALTARVDATLARDRAPERYRAELREIGTDLTRLSSLANHLLLLARDPAALARE
AVPLRDLAADAVDRARELAPEADVDLIAPQPVGVAGDRVLLGQAIWNLTVNAVLHAPGATVTVTVRDDGEGGGVVEVRDD
GPGVDAAVLARLGEAFYRPDASRTASGGARGHGLGLALVRRAAELHGGTLRLESAPGAGFCATLHLPARSSTGEVLRSPA

Sequences:

>Translated_480_residues
MRPRGIRLAFPLTLRAQLAWWAALATALAVALVAAGLFVAVNSFLFAAQRDRLAAAVGAVQARVETALGRGDDLLAALLG
VQGTLAPADLEAILDADPQTRHLDVRLITVGSGQVRVLPTPRFPDGIPLNLPPGTYREGDRLVAVRVLAGGRAALTVVSD
ARALGEAQAAFGRALWGLVPLALLLSLALGWTVAGRLLAPVRTLEMAAREIGASGNLRRPVPGAGERDELARLALTLQET
FIRLADARDRQQDFLRAAAHDLRSPLAALTARVDATLARDRAPERYRAELREIGTDLTRLSSLANHLLLLARDPAALARE
AVPLRDLAADAVDRARELAPEADVDLIAPQPVGVAGDRVLLGQAIWNLTVNAVLHAPGATVTVTVRDDGEGGGVVEVRDD
GPGVDAAVLARLGEAFYRPDASRTASGGARGHGLGLALVRRAAELHGGTLRLESAPGAGFCATLHLPARSSTGEVLRSPA
>Mature_480_residues
MRPRGIRLAFPLTLRAQLAWWAALATALAVALVAAGLFVAVNSFLFAAQRDRLAAAVGAVQARVETALGRGDDLLAALLG
VQGTLAPADLEAILDADPQTRHLDVRLITVGSGQVRVLPTPRFPDGIPLNLPPGTYREGDRLVAVRVLAGGRAALTVVSD
ARALGEAQAAFGRALWGLVPLALLLSLALGWTVAGRLLAPVRTLEMAAREIGASGNLRRPVPGAGERDELARLALTLQET
FIRLADARDRQQDFLRAAAHDLRSPLAALTARVDATLARDRAPERYRAELREIGTDLTRLSSLANHLLLLARDPAALARE
AVPLRDLAADAVDRARELAPEADVDLIAPQPVGVAGDRVLLGQAIWNLTVNAVLHAPGATVTVTVRDDGEGGGVVEVRDD
GPGVDAAVLARLGEAFYRPDASRTASGGARGHGLGLALVRRAAELHGGTLRLESAPGAGFCATLHLPARSSTGEVLRSPA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789403, Length=296, Percent_Identity=32.7702702702703, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790346, Length=279, Percent_Identity=32.6164874551971, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786783, Length=344, Percent_Identity=27.3255813953488, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=30.2491103202847, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788279, Length=236, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=30.1587301587302, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=305, Percent_Identity=29.8360655737705, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790551, Length=292, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789149, Length=291, Percent_Identity=25.7731958762887, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081816, Length=125, Percent_Identity=36.8, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=30.2752293577982, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=324, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=25.9515570934256, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50273; Mature: 50273

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRGIRLAFPLTLRAQLAWWAALATALAVALVAAGLFVAVNSFLFAAQRDRLAAAVGAV
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARVETALGRGDDLLAALLGVQGTLAPADLEAILDADPQTRHLDVRLITVGSGQVRVLPT
HHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEC
PRFPDGIPLNLPPGTYREGDRLVAVRVLAGGRAALTVVSDARALGEAQAAFGRALWGLVP
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LALLLSLALGWTVAGRLLAPVRTLEMAAREIGASGNLRRPVPGAGERDELARLALTLQET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FIRLADARDRQQDFLRAAAHDLRSPLAALTARVDATLARDRAPERYRAELREIGTDLTRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SSLANHLLLLARDPAALAREAVPLRDLAADAVDRARELAPEADVDLIAPQPVGVAGDRVL
HHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHH
LGQAIWNLTVNAVLHAPGATVTVTVRDDGEGGGVVEVRDDGPGVDAAVLARLGEAFYRPD
HHHHHHHHHEEHEEECCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
ASRTASGGARGHGLGLALVRRAAELHGGTLRLESAPGAGFCATLHLPARSSTGEVLRSPA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCHHHHCCCC
>Mature Secondary Structure
MRPRGIRLAFPLTLRAQLAWWAALATALAVALVAAGLFVAVNSFLFAAQRDRLAAAVGAV
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARVETALGRGDDLLAALLGVQGTLAPADLEAILDADPQTRHLDVRLITVGSGQVRVLPT
HHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEC
PRFPDGIPLNLPPGTYREGDRLVAVRVLAGGRAALTVVSDARALGEAQAAFGRALWGLVP
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LALLLSLALGWTVAGRLLAPVRTLEMAAREIGASGNLRRPVPGAGERDELARLALTLQET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FIRLADARDRQQDFLRAAAHDLRSPLAALTARVDATLARDRAPERYRAELREIGTDLTRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SSLANHLLLLARDPAALAREAVPLRDLAADAVDRARELAPEADVDLIAPQPVGVAGDRVL
HHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHH
LGQAIWNLTVNAVLHAPGATVTVTVRDDGEGGGVVEVRDDGPGVDAAVLARLGEAFYRPD
HHHHHHHHHEEHEEECCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
ASRTASGGARGHGLGLALVRRAAELHGGTLRLESAPGAGFCATLHLPARSSTGEVLRSPA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]