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The map label for this gene is mdh

Identifier: 94986260

GI number: 94986260

Start: 2277542

End: 2278546

Strand: Reverse

Name: mdh

Synonym: Dgeo_2161

Alternate gene names: 94986260

Gene position: 2278546-2277542 (Counterclockwise)

Preceding gene: 94986261

Following gene: 94986258

Centisome position: 92.35

GC content: 64.98

Gene sequence:

>1005_bases
ATGACGATGAACCAAGGCACCAAGCAACCCGTTCGCGTGGCCGTGACCGGCGCCGCCGGGCAGATCGGCTACAGCCTGCT
TTTTCGCATTGCGGCGGGCGACATGCTGGGCAAGGATCAGCCGGTGATCCTACAGTTGCTGGAGATCACGCCTGCCCTCA
AGGCGTTGGCGGGCGTCGTGATGGAGCTGCGCGACTGCGCTTTCCCGTTGCTGGCGGATATCGTGACCAGTGACGATCCG
CTGGTGGCCTTTAAGGACGCGGACTACGCCCTCCTCGTCGGTGCTATGCCGCGCAAGGCCGGGATGGAGCGCGGCGACCT
GCTGGGCGCGAACGGCGGCATCTTCAAGCCGCAGGGCGAGGCGCTGAACAAGGTGGCGAGCCGAGACGTGAAGGTGCTCG
TGGTGGGGAACCCCGCCAACACCAACGCCCTGATCGCCCAGCAGAACGCGCCTGACCTCGATCCCAAGCAGTTCACCGCG
ATGGTGCGTCTGGACCACAACCGCGCGATCTCGCAGCTTGCCGAGAAGACCGGCCAGCCCGTGAGTGCCATCAAGAACAT
CACCATCTGGGGGAACCACTCTTCCACCCAGTACCCCGACCTCTCGCAGGCGACCGTGAACGGCCAGCCCGCCCTCGACC
TGGTGGACCGCGAGTGGTACGAGAAAGAATACATTCCGACGGTCGCCAAGCGTGGCGCGGCGATCATCGAGGCGCGTGGG
GCCAGCTCTGCCGCTTCTGCCGCCTCCGCTGCGATTGACCACATGCGCGACTGGGCGCTGGGCACCCCGGAAGGCGAGTG
GGTCAGCATGGCGGTGCCCAGCGACGGCTCCTACGGCATTCCTGAGGGTTTGATCTACGGCTTCCCGGTGCGTTGCCGCA
ACGGCCAGTACGAGATCGTGCAGGGCCTCGAGATCAGTGACTTCAGCCGCCAGAAGATGGACGCCACCGCCAAGGAACTG
GAAGAAGAGCGCGAAGAAGTGCGTCGGCTTGGTCTGGTGAAGTAA

Upstream 100 bases:

>100_bases
ACGCTTCCCCCCTGTCCCCCCCACGGGAACGCCCCCTCCCCACCTTCCCCCTCCGCGTTATTGTTGGTCGAAAAACTTCA
CCTTCCGGGAGGACATCACC

Downstream 100 bases:

>100_bases
GGCAGGGAGGAGGCGCGCAGCCTTGCCGCTGCTGCGCCTCTCGTTCGCCGGGCTGCTTCCCGCAGAAACAACGCTCCCTG
TTGACAGTTCCGGGAGGCAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTMNQGTKQPVRVAVTGAAGQIGYSLLFRIAAGDMLGKDQPVILQLLEITPALKALAGVVMELRDCAFPLLADIVTSDDP
LVAFKDADYALLVGAMPRKAGMERGDLLGANGGIFKPQGEALNKVASRDVKVLVVGNPANTNALIAQQNAPDLDPKQFTA
MVRLDHNRAISQLAEKTGQPVSAIKNITIWGNHSSTQYPDLSQATVNGQPALDLVDREWYEKEYIPTVAKRGAAIIEARG
ASSAASAASAAIDHMRDWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIVQGLEISDFSRQKMDATAKEL
EEEREEVRRLGLVK

Sequences:

>Translated_334_residues
MTMNQGTKQPVRVAVTGAAGQIGYSLLFRIAAGDMLGKDQPVILQLLEITPALKALAGVVMELRDCAFPLLADIVTSDDP
LVAFKDADYALLVGAMPRKAGMERGDLLGANGGIFKPQGEALNKVASRDVKVLVVGNPANTNALIAQQNAPDLDPKQFTA
MVRLDHNRAISQLAEKTGQPVSAIKNITIWGNHSSTQYPDLSQATVNGQPALDLVDREWYEKEYIPTVAKRGAAIIEARG
ASSAASAASAAIDHMRDWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIVQGLEISDFSRQKMDATAKEL
EEEREEVRRLGLVK
>Mature_333_residues
TMNQGTKQPVRVAVTGAAGQIGYSLLFRIAAGDMLGKDQPVILQLLEITPALKALAGVVMELRDCAFPLLADIVTSDDPL
VAFKDADYALLVGAMPRKAGMERGDLLGANGGIFKPQGEALNKVASRDVKVLVVGNPANTNALIAQQNAPDLDPKQFTAM
VRLDHNRAISQLAEKTGQPVSAIKNITIWGNHSSTQYPDLSQATVNGQPALDLVDREWYEKEYIPTVAKRGAAIIEARGA
SSAASAASAAIDHMRDWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIVQGLEISDFSRQKMDATAKELE
EEREEVRRLGLVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=323, Percent_Identity=57.2755417956656, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI89886456, Length=329, Percent_Identity=25.2279635258359, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789632, Length=277, Percent_Identity=25.9927797833935, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=55.521472392638, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI32566798, Length=259, Percent_Identity=54.8262548262548, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32566800, Length=174, Percent_Identity=54.0229885057471, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24583394, Length=323, Percent_Identity=58.2043343653251, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_DEIGD (Q1IWC9)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605624.1
- ProteinModelPortal:   Q1IWC9
- SMR:   Q1IWC9
- STRING:   Q1IWC9
- GeneID:   4058896
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2161
- NMPDR:   fig|68909.1.peg.1991
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   DGEO319795:DGEO_2161-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011274
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759
- TIGRFAMs:   TIGR01758

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35928; Mature: 35797

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 193-193 BINDING 98-98 BINDING 104-104 BINDING 111-111 BINDING 118-118 BINDING 137-137 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNQGTKQPVRVAVTGAAGQIGYSLLFRIAAGDMLGKDQPVILQLLEITPALKALAGVV
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
MELRDCAFPLLADIVTSDDPLVAFKDADYALLVGAMPRKAGMERGDLLGANGGIFKPQGE
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCHHCCCCCCCEEECCCCCCCCCCH
ALNKVASRDVKVLVVGNPANTNALIAQQNAPDLDPKQFTAMVRLDHNRAISQLAEKTGQP
HHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEEEECCHHHHHHHHHHCCCC
VSAIKNITIWGNHSSTQYPDLSQATVNGQPALDLVDREWYEKEYIPTVAKRGAAIIEARG
HHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECC
ASSAASAASAAIDHMRDWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIV
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEECCCEEECCCCEEEE
QGLEISDFSRQKMDATAKELEEEREEVRRLGLVK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMNQGTKQPVRVAVTGAAGQIGYSLLFRIAAGDMLGKDQPVILQLLEITPALKALAGVV
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
MELRDCAFPLLADIVTSDDPLVAFKDADYALLVGAMPRKAGMERGDLLGANGGIFKPQGE
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCHHCCCCCCCEEECCCCCCCCCCH
ALNKVASRDVKVLVVGNPANTNALIAQQNAPDLDPKQFTAMVRLDHNRAISQLAEKTGQP
HHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEEEECCHHHHHHHHHHCCCC
VSAIKNITIWGNHSSTQYPDLSQATVNGQPALDLVDREWYEKEYIPTVAKRGAAIIEARG
HHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECC
ASSAASAASAAIDHMRDWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIV
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEECCCEEECCCCEEEE
QGLEISDFSRQKMDATAKELEEEREEVRRLGLVK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA