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The map label for this gene is gatA [H]

Identifier: 94985434

GI number: 94985434

Start: 1416399

End: 1417805

Strand: Reverse

Name: gatA [H]

Synonym: Dgeo_1333

Alternate gene names: 94985434

Gene position: 1417805-1416399 (Counterclockwise)

Preceding gene: 94985436

Following gene: 94985433

Centisome position: 57.47

GC content: 70.43

Gene sequence:

>1407_bases
ATGCCTGACCCCGTTCTTGATCTCGACGCCGCCTCACTGAGCGCCGCCATACGCCGCGGCGACCTGACCGCCCTCGAAGT
CACCCGGGCCTACCTCTCACGCATCGCGGCCCACAATCCCCGCCTTCACGCGGTCATCGCTGTGAACGGGGCGGCGGAGG
CGGACGCCGAACGTTTGGACGCGCTGCCCGAGCATCAGCGTGGCCCGCTGCACGGCGTGCCTCTCTTGATCAAGGACAAC
ATCGATGTGGCGGGGTTGCCCACCACCGCCGGAAGCCTGCTGATGACGCGGCATGTACCCAAGGCCGATGCGCCGCTGGT
CGCCCGCCTGCGTGCGGCGGGGGCTGTGATCCTGGGGAAGGCCAATCTGACCGAATGGGCCAACTTCATGACGCTGGGCA
TGCCCAACGGGTACTCGTCGGCAGGGGGGCAGACCGTGAACCCCTGGGGTGAGGGGCTGGACACCGGCGGCAGCTCCAGC
GGGAGCGGCGTGGCGGTGGCGGCTCGGCTGTGTGTGGCCGCCATCGGGACCGAGACGAGCGGCAGCATCCTGAGTCCCGC
GCAGCAGAACGGGGTGATCGGCCTTAAGCCCACGGTGGGCCTGATTCCGCGCACGGGCATTGTCCCCATCAGCCACAGCC
AGGACACTGCCGGGCCGCTCACCCGCACCGTCCGGGACGCGGCCCTGCTGCTCGGAGTGATGGCTGGACCCGACGACGCT
GACCCGGCCAGCCGCCGCCTGCCGGTGCCCGACCTGACGCTGCAAGCGGATGCCCTGGCGGGGGCGCAGATCGGCGTCTT
GCGTGACCCGCCGGGCGGTCCCGTGAGCGAGGCTGAACGTGCGTCCCTTGCGCGCGCCGAGGCCGCCCTGACCCTGGCGG
GCGCGACCCTGCAGGACGTGACGCTGGAAAGTGGCCCCGAACTCAGCAACTGGCGACTGGAGGTGCTTGTCTACGAATTC
AAACATGACCTGAACGCCTACCTCGCGAGGGTGCAGGACGGCCCACGCAGCCTGGCGGAGATCATCGAGGCGAACGACGC
CGACCCCGAACGGCTCCAGCGCTACGGCCAGACGCTGCTCTATGCGGCGCAGGCTACCCGTGGCGACCTCAGCGAGCGGG
CTTACCGTGAGGCACGCGCCCGTGACCTTGACCAGACACGAACGCGTGGGCTTGACCCCCTCTTTGCCCTGGGCCTCGAC
GCGCTGCTGTGGCCCGGCCTGCACGGCTACGCGGTGGGCGCGAAGGCCGGGTATCCCAGCGTGACGCTGCCAACCGGCTT
GCAAGCGGGCGAGCCAACGGGTGTGCTGCTGACTGGCCCGGCGGGGAGTGACGGCCGCCTGCTGGCACTGGCCGCCGACC
TGAACCGCTGCCTCGGTGGCGTGCAGTTCCCCCCCGACCCCGCTTGA

Upstream 100 bases:

>100_bases
TGCCCCTTGCCACTCATGGGGCGCAGGCTAGCGCACCGCCTCCGAGCGGGCGGGACGTGGGGGAGATGTGGCCTAGACTG
CCTGTGTGTCCCCCCTGCAG

Downstream 100 bases:

>100_bases
CCCCGCGCCCACACCCGACTAGCATAGGCGGGCTGCCTCTGTGGGGCAGGTGCGGGCGGTTAGCTCAGCGGTAGAGCGTC
CGCCTTACAAGCGGAGGGTC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPDPVLDLDAASLSAAIRRGDLTALEVTRAYLSRIAAHNPRLHAVIAVNGAAEADAERLDALPEHQRGPLHGVPLLIKDN
IDVAGLPTTAGSLLMTRHVPKADAPLVARLRAAGAVILGKANLTEWANFMTLGMPNGYSSAGGQTVNPWGEGLDTGGSSS
GSGVAVAARLCVAAIGTETSGSILSPAQQNGVIGLKPTVGLIPRTGIVPISHSQDTAGPLTRTVRDAALLLGVMAGPDDA
DPASRRLPVPDLTLQADALAGAQIGVLRDPPGGPVSEAERASLARAEAALTLAGATLQDVTLESGPELSNWRLEVLVYEF
KHDLNAYLARVQDGPRSLAEIIEANDADPERLQRYGQTLLYAAQATRGDLSERAYREARARDLDQTRTRGLDPLFALGLD
ALLWPGLHGYAVGAKAGYPSVTLPTGLQAGEPTGVLLTGPAGSDGRLLALAADLNRCLGGVQFPPDPA

Sequences:

>Translated_468_residues
MPDPVLDLDAASLSAAIRRGDLTALEVTRAYLSRIAAHNPRLHAVIAVNGAAEADAERLDALPEHQRGPLHGVPLLIKDN
IDVAGLPTTAGSLLMTRHVPKADAPLVARLRAAGAVILGKANLTEWANFMTLGMPNGYSSAGGQTVNPWGEGLDTGGSSS
GSGVAVAARLCVAAIGTETSGSILSPAQQNGVIGLKPTVGLIPRTGIVPISHSQDTAGPLTRTVRDAALLLGVMAGPDDA
DPASRRLPVPDLTLQADALAGAQIGVLRDPPGGPVSEAERASLARAEAALTLAGATLQDVTLESGPELSNWRLEVLVYEF
KHDLNAYLARVQDGPRSLAEIIEANDADPERLQRYGQTLLYAAQATRGDLSERAYREARARDLDQTRTRGLDPLFALGLD
ALLWPGLHGYAVGAKAGYPSVTLPTGLQAGEPTGVLLTGPAGSDGRLLALAADLNRCLGGVQFPPDPA
>Mature_467_residues
PDPVLDLDAASLSAAIRRGDLTALEVTRAYLSRIAAHNPRLHAVIAVNGAAEADAERLDALPEHQRGPLHGVPLLIKDNI
DVAGLPTTAGSLLMTRHVPKADAPLVARLRAAGAVILGKANLTEWANFMTLGMPNGYSSAGGQTVNPWGEGLDTGGSSSG
SGVAVAARLCVAAIGTETSGSILSPAQQNGVIGLKPTVGLIPRTGIVPISHSQDTAGPLTRTVRDAALLLGVMAGPDDAD
PASRRLPVPDLTLQADALAGAQIGVLRDPPGGPVSEAERASLARAEAALTLAGATLQDVTLESGPELSNWRLEVLVYEFK
HDLNAYLARVQDGPRSLAEIIEANDADPERLQRYGQTLLYAAQATRGDLSERAYREARARDLDQTRTRGLDPLFALGLDA
LLWPGLHGYAVGAKAGYPSVTLPTGLQAGEPTGVLLTGPAGSDGRLLALAADLNRCLGGVQFPPDPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=28.7128712871287, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI195972892, Length=254, Percent_Identity=33.4645669291339, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=25.2941176470588, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=209, Percent_Identity=33.0143540669856, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=30.6532663316583, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=244, Percent_Identity=31.1475409836066, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=242, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=242, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=178, Percent_Identity=41.0112359550562, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=217, Percent_Identity=30.8755760368664, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=28.8702928870293, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648113, Length=243, Percent_Identity=38.2716049382716, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24644968, Length=253, Percent_Identity=31.2252964426877, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=29.4354838709677, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=29.4354838709677, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=29.4354838709677, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24652985, Length=261, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922090, Length=261, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652981, Length=261, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652983, Length=261, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=30.2904564315353, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=26.0038240917782, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648441, Length=178, Percent_Identity=31.4606741573034, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=178, Percent_Identity=31.4606741573034, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 48475; Mature: 48344

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDPVLDLDAASLSAAIRRGDLTALEVTRAYLSRIAAHNPRLHAVIAVNGAAEADAERLD
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ALPEHQRGPLHGVPLLIKDNIDVAGLPTTAGSLLMTRHVPKADAPLVARLRAAGAVILGK
CCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCEEEEEC
ANLTEWANFMTLGMPNGYSSAGGQTVNPWGEGLDTGGSSSGSGVAVAARLCVAAIGTETS
CCHHHHHHEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GSILSPAQQNGVIGLKPTVGLIPRTGIVPISHSQDTAGPLTRTVRDAALLLGVMAGPDDA
CCCCCCHHHCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DPASRRLPVPDLTLQADALAGAQIGVLRDPPGGPVSEAERASLARAEAALTLAGATLQDV
CCCCCCCCCCCCEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCEEE
TLESGPELSNWRLEVLVYEFKHDLNAYLARVQDGPRSLAEIIEANDADPERLQRYGQTLL
ECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHE
YAAQATRGDLSERAYREARARDLDQTRTRGLDPLFALGLDALLWPGLHGYAVGAKAGYPS
EHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCC
VTLPTGLQAGEPTGVLLTGPAGSDGRLLALAADLNRCLGGVQFPPDPA
EECCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PDPVLDLDAASLSAAIRRGDLTALEVTRAYLSRIAAHNPRLHAVIAVNGAAEADAERLD
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ALPEHQRGPLHGVPLLIKDNIDVAGLPTTAGSLLMTRHVPKADAPLVARLRAAGAVILGK
CCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCEEEEEC
ANLTEWANFMTLGMPNGYSSAGGQTVNPWGEGLDTGGSSSGSGVAVAARLCVAAIGTETS
CCHHHHHHEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GSILSPAQQNGVIGLKPTVGLIPRTGIVPISHSQDTAGPLTRTVRDAALLLGVMAGPDDA
CCCCCCHHHCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DPASRRLPVPDLTLQADALAGAQIGVLRDPPGGPVSEAERASLARAEAALTLAGATLQDV
CCCCCCCCCCCCEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCEEE
TLESGPELSNWRLEVLVYEFKHDLNAYLARVQDGPRSLAEIIEANDADPERLQRYGQTLL
ECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHE
YAAQATRGDLSERAYREARARDLDQTRTRGLDPLFALGLDALLWPGLHGYAVGAKAGYPS
EHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCC
VTLPTGLQAGEPTGVLLTGPAGSDGRLLALAADLNRCLGGVQFPPDPA
EECCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA