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The map label for this gene is mhqO [H]

Identifier: 94985047

GI number: 94985047

Start: 1005063

End: 1006721

Strand: Reverse

Name: mhqO [H]

Synonym: Dgeo_0941

Alternate gene names: 94985047

Gene position: 1006721-1005063 (Counterclockwise)

Preceding gene: 94985048

Following gene: 94985042

Centisome position: 40.8

GC content: 69.2

Gene sequence:

>1659_bases
ATGGCAAACACCCTCTCGCCCATTCCCGGCACCACGCCGGTGCAGGGTCTGCATCACGTCACGGTGATGGCCTCGGATCC
TCAGCGCAACATCGACTTCTACACGCAGACGCTGGGGCAGCGGCTCGTCAAGGTGACGGTGAATTTCGACGATCCCGGTA
CCTACCACTTCTACTACGGCGACCTCACCGGGCAGCCGGGCACGATCATGACTCACTTTCCCTGGCCAGGCGCGAAGAGG
GGCACCCGCGGCAACGGCGAGGTCGTGGCGACGGCCTACAGCGTTCCCCGCACGAGCGAGGCCTACTGGAAGGCCCGGCT
GGCAGGACAGGGTGTCGCCTTCCGCGAGGGCGTGCGTTTTGGCGAGCAAGTCCTGACGTTTGAGGATCCAGACGGCACCT
GGGTCGAACTGGTGTTCGAAGACAGCCCGTCCGTCCAACCCTGGCCCGCCAGCCCAGTGCCCGCCGAATACGCCCTGCGC
GGCTTTCACTCGGTCACGGCCTGGGTGCGGGAGACAGCGCCCGTCCGCGACCTGTTGGTGGGCCAGCTCGGCTTCCGCGA
GGGAGGAAGCGAGCCGGACACGGAAGGCACCCGCACCCGCTTTCGGGGCAGCGGCGAAGGCGTGGGCCTGTTTGTGGACG
TGGTCGAGCGGCCCGGCCAGCCGCGTGGCCATTTCGGCGCGGGGAGTATCCACCACGTCGCCCTGCGGACCCGCGACGAC
ACCGAACAGGAGGCGTACCTCGCGGGCCTGAGCGCGGCAGGCTACCGACCCACACCGGTCCAGGACCGCCAGTATTTCCA
CTCCATCTACTTCCGCGAGCCGAACGGCGTGCTGTTTGAGATCGCCACCGATGCCCCCGGCTTCCCGGACGACGAACCGG
TGGAGGAACTGGGCCGGCACCTCAAGCTGCCCACGTGGTACGAACCGCAACGCGCAGCCATCGAGGCCCACTTGCCCAAG
ATCATCAACCGCGAGTACGGCGTGACCATCGGGACGCGGGACCTGCAGGCGGGGGACGGGGCAGCTCAGGCTGCACCCGC
GGTCCAGGGCGTGCAGGTGTATGCGGCGGGCCGGCCGCTGAAAGAGGCGCGAGTGGCGATGGTGCTGCTGCACGGGCGGG
GCGGCAGCGCGCCCGATATGCTCGCGCTGGCGGACGAGCTGAACCTCAGCGCCTTCGCGTATCTCGCCCCTCAGGCCGAG
GGGAACAGCTGGTATCCGCGGTCCTTTCTCGCCCCCGCCCAGCAGAACCAGCCCCACCTCGACCGCGCCCTTGCCACCGT
GGACGCTGTGCTGAATGAGCTGGAGGCGCAGGGGATCTCGGCGCAACAGGTGGTGCTGGGCGGCTTCTCGCAAGGCGCAT
GTCTCGCGCTGGAGTACGCCAGCCGCACGGGCCGGAGGCTGGGCGGCGTGGTGGCCTTCAGCGGCGCCCTGATCACACTG
AACCAGACCGGGAACCTGCCCGGCACGCCCGTCTTCATGGGGGTCGCCCCCGACGACGCACACATTCCGCTCGCGCGCTT
CGAGGAGAGCGCGGCGCAACTCCGCGCCCGGGGCGCACAGGTGGACGCGCGCGTCTATCCCGGCCTGGGCCACAGCATCA
ACCAGGATGAGCTGGACGCCGCCCGCCGGATCATGCAGCGGGTGGCTGGGCAGCTCTGA

Upstream 100 bases:

>100_bases
GCGCCGGTCCGACGCAGACAGCACACCTGACGCACCGGCCGCAGGCGGTCGGTGGTCCAGCTCACTCCGAATCATTTAAC
ATTAAGTTATTTTGGGGGGT

Downstream 100 bases:

>100_bases
GGGCAAGCGGGAGAACGCCCAGCGCTGCCCCCTGCACTACTGCAACCGCGCCGCCACGATGACGAGGTCGGCGTTTGGCC
CCTCAAAACCGGGGGTATAG

Product: phospholipase/carboxylesterase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MANTLSPIPGTTPVQGLHHVTVMASDPQRNIDFYTQTLGQRLVKVTVNFDDPGTYHFYYGDLTGQPGTIMTHFPWPGAKR
GTRGNGEVVATAYSVPRTSEAYWKARLAGQGVAFREGVRFGEQVLTFEDPDGTWVELVFEDSPSVQPWPASPVPAEYALR
GFHSVTAWVRETAPVRDLLVGQLGFREGGSEPDTEGTRTRFRGSGEGVGLFVDVVERPGQPRGHFGAGSIHHVALRTRDD
TEQEAYLAGLSAAGYRPTPVQDRQYFHSIYFREPNGVLFEIATDAPGFPDDEPVEELGRHLKLPTWYEPQRAAIEAHLPK
IINREYGVTIGTRDLQAGDGAAQAAPAVQGVQVYAAGRPLKEARVAMVLLHGRGGSAPDMLALADELNLSAFAYLAPQAE
GNSWYPRSFLAPAQQNQPHLDRALATVDAVLNELEAQGISAQQVVLGGFSQGACLALEYASRTGRRLGGVVAFSGALITL
NQTGNLPGTPVFMGVAPDDAHIPLARFEESAAQLRARGAQVDARVYPGLGHSINQDELDAARRIMQRVAGQL

Sequences:

>Translated_552_residues
MANTLSPIPGTTPVQGLHHVTVMASDPQRNIDFYTQTLGQRLVKVTVNFDDPGTYHFYYGDLTGQPGTIMTHFPWPGAKR
GTRGNGEVVATAYSVPRTSEAYWKARLAGQGVAFREGVRFGEQVLTFEDPDGTWVELVFEDSPSVQPWPASPVPAEYALR
GFHSVTAWVRETAPVRDLLVGQLGFREGGSEPDTEGTRTRFRGSGEGVGLFVDVVERPGQPRGHFGAGSIHHVALRTRDD
TEQEAYLAGLSAAGYRPTPVQDRQYFHSIYFREPNGVLFEIATDAPGFPDDEPVEELGRHLKLPTWYEPQRAAIEAHLPK
IINREYGVTIGTRDLQAGDGAAQAAPAVQGVQVYAAGRPLKEARVAMVLLHGRGGSAPDMLALADELNLSAFAYLAPQAE
GNSWYPRSFLAPAQQNQPHLDRALATVDAVLNELEAQGISAQQVVLGGFSQGACLALEYASRTGRRLGGVVAFSGALITL
NQTGNLPGTPVFMGVAPDDAHIPLARFEESAAQLRARGAQVDARVYPGLGHSINQDELDAARRIMQRVAGQL
>Mature_551_residues
ANTLSPIPGTTPVQGLHHVTVMASDPQRNIDFYTQTLGQRLVKVTVNFDDPGTYHFYYGDLTGQPGTIMTHFPWPGAKRG
TRGNGEVVATAYSVPRTSEAYWKARLAGQGVAFREGVRFGEQVLTFEDPDGTWVELVFEDSPSVQPWPASPVPAEYALRG
FHSVTAWVRETAPVRDLLVGQLGFREGGSEPDTEGTRTRFRGSGEGVGLFVDVVERPGQPRGHFGAGSIHHVALRTRDDT
EQEAYLAGLSAAGYRPTPVQDRQYFHSIYFREPNGVLFEIATDAPGFPDDEPVEELGRHLKLPTWYEPQRAAIEAHLPKI
INREYGVTIGTRDLQAGDGAAQAAPAVQGVQVYAAGRPLKEARVAMVLLHGRGGSAPDMLALADELNLSAFAYLAPQAEG
NSWYPRSFLAPAQQNQPHLDRALATVDAVLNELEAQGISAQQVVLGGFSQGACLALEYASRTGRRLGGVVAFSGALITLN
QTGNLPGTPVFMGVAPDDAHIPLARFEESAAQLRARGAQVDARVYPGLGHSINQDELDAARRIMQRVAGQL

Specific function: Putative ring-cleavage dioxygenase that may contribute to the degradation of aromatic compounds (Potential) [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 59707; Mature: 59575

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTLSPIPGTTPVQGLHHVTVMASDPQRNIDFYTQTLGQRLVKVTVNFDDPGTYHFYYG
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEC
DLTGQPGTIMTHFPWPGAKRGTRGNGEVVATAYSVPRTSEAYWKARLAGQGVAFREGVRF
CCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHC
GEQVLTFEDPDGTWVELVFEDSPSVQPWPASPVPAEYALRGFHSVTAWVRETAPVRDLLV
CCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GQLGFREGGSEPDTEGTRTRFRGSGEGVGLFVDVVERPGQPRGHFGAGSIHHVALRTRDD
HHHCCCCCCCCCCCCCCHHEECCCCCCCEEEEEEHHCCCCCCCCCCCCCEEEEEEECCCC
TEQEAYLAGLSAAGYRPTPVQDRQYFHSIYFREPNGVLFEIATDAPGFPDDEPVEELGRH
CHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHH
LKLPTWYEPQRAAIEAHLPKIINREYGVTIGTRDLQAGDGAAQAAPAVQGVQVYAAGRPL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCH
KEARVAMVLLHGRGGSAPDMLALADELNLSAFAYLAPQAEGNSWYPRSFLAPAQQNQPHL
HCCEEEEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCHH
DRALATVDAVLNELEAQGISAQQVVLGGFSQGACLALEYASRTGRRLGGVVAFSGALITL
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHCCHHHCCEEEECCEEEEE
NQTGNLPGTPVFMGVAPDDAHIPLARFEESAAQLRARGAQVDARVYPGLGHSINQDELDA
ECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ARRIMQRVAGQL
HHHHHHHHHCCC
>Mature Secondary Structure 
ANTLSPIPGTTPVQGLHHVTVMASDPQRNIDFYTQTLGQRLVKVTVNFDDPGTYHFYYG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEC
DLTGQPGTIMTHFPWPGAKRGTRGNGEVVATAYSVPRTSEAYWKARLAGQGVAFREGVRF
CCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHC
GEQVLTFEDPDGTWVELVFEDSPSVQPWPASPVPAEYALRGFHSVTAWVRETAPVRDLLV
CCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GQLGFREGGSEPDTEGTRTRFRGSGEGVGLFVDVVERPGQPRGHFGAGSIHHVALRTRDD
HHHCCCCCCCCCCCCCCHHEECCCCCCCEEEEEEHHCCCCCCCCCCCCCEEEEEEECCCC
TEQEAYLAGLSAAGYRPTPVQDRQYFHSIYFREPNGVLFEIATDAPGFPDDEPVEELGRH
CHHHHHHHCCCCCCCCCCCCCHHHHEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHH
LKLPTWYEPQRAAIEAHLPKIINREYGVTIGTRDLQAGDGAAQAAPAVQGVQVYAAGRPL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCH
KEARVAMVLLHGRGGSAPDMLALADELNLSAFAYLAPQAEGNSWYPRSFLAPAQQNQPHL
HCCEEEEEEEECCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCHH
DRALATVDAVLNELEAQGISAQQVVLGGFSQGACLALEYASRTGRRLGGVVAFSGALITL
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHCCHHHCCEEEECCEEEEE
NQTGNLPGTPVFMGVAPDDAHIPLARFEESAAQLRARGAQVDARVYPGLGHSINQDELDA
ECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ARRIMQRVAGQL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]