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The map label for this gene is pilT [H]

Identifier: 94984776

GI number: 94984776

Start: 727044

End: 728120

Strand: Reverse

Name: pilT [H]

Synonym: Dgeo_0669

Alternate gene names: 94984776

Gene position: 728120-727044 (Counterclockwise)

Preceding gene: 94984777

Following gene: 94984775

Centisome position: 29.51

GC content: 65.18

Gene sequence:

>1077_bases
GTGACCCTCGACGAGCTGCTGCGCGAGATGGTGGGCCGCCGCGCCTCAGACGTGCATCTCCAGGTGGGCAGCCCGCCCAT
GGGCCGCATCGACGGCAGCCTGCTGCCCTTCGGTACCCAGCCGCTCACGCCCTCCGATACGGTGGCACTGGCACGTGCCC
TCCTCACGCCCGAGCAGTGGGAGGAATTCGAATACCGCAACGAGCTTGACCTCGCCTACAGCGTGCACGGCCTCGGCCGC
TTCCGCTGCAACGTGTTCCGGCAGCGTGGGGCGGTCGGGATCGTCATGCGCACCGTGAACGACGCCATTCCCAGCTTTGA
GGCCCTGGGCCTGCCTGCCGACGTGCTGCGCGGCTTCGCGGCGACCTCACGTGGGCTGCTGCTGATCACCGGGCCCACCA
GCAGCGGGAAAAGCACCACCCTGGCCGCCCTGATTGACCACATCAACCGCCACTTCGCCTACAACATCCTCACCATTGAG
GACCCCATCGAGTTTCTGCACCGCAATCACCGGAGTCTGGTGGTGCAGCGCGAGGTGGGATCAGACACCCGCGACTTCCG
CACTGCCCTGAAATACGCGCTGCGCCAGGATCCCGACGTGATTATGATTGGAGAGATGCGCGACCGGGAAACCGTGGAGG
CGGCGCTCTCCGCCGCGCAGACCGGGCACTTGGTGCTGAGTACGCTCCACACGCAGGACGCCGTACGCAGCGTGAACCGC
ATCATCGACTTCTTCCCGCCCCACGAACGCACCCAGGTGCGGCTCCAGCTCGCGGAGTCGCTGGTCGGCATCGTCAGCCA
ACGCCTGGTGCGCCGCGCCGATGGCGAAGGCCGAGTGCTGGCCACCGAGATTCTGATCAACACTCCTCTGGTACAGGACT
ACATCAAGGACGAGGACAAAACCCCGCTGATCAAGGAGGCGCTGCTCGAGGACAACATCCGCGGGATGCAGACCTTCGAC
CAGCATCTGATGCAACTTTACCGCCACCACCTGATCACGCTCGAGGAGGCTCTCCACCACGCCACCAGTCCCCATGAGCT
GCGGCTGATGATCACCCGAGCGGGCTTTCTTTCCTGA

Upstream 100 bases:

>100_bases
GCGTGCTGGTGGCCGCCCGGCTGTTCGGCGTTCGGCTGATCGACAACGTCCCCCTGTCTCCAGCAGAACGGCGACTATCG
GAACGGGAGAGCCGCAACAC

Downstream 100 bases:

>100_bases
ATTCCCCGCGCAGCCTACCCTATACTGGGCCGCCATGACCAAGACGCGCATTCCCATGACCAAACGTGGCTATGACAAGC
TGCGCGAAACCCTGGAATAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTLDELLREMVGRRASDVHLQVGSPPMGRIDGSLLPFGTQPLTPSDTVALARALLTPEQWEEFEYRNELDLAYSVHGLGR
FRCNVFRQRGAVGIVMRTVNDAIPSFEALGLPADVLRGFAATSRGLLLITGPTSSGKSTTLAALIDHINRHFAYNILTIE
DPIEFLHRNHRSLVVQREVGSDTRDFRTALKYALRQDPDVIMIGEMRDRETVEAALSAAQTGHLVLSTLHTQDAVRSVNR
IIDFFPPHERTQVRLQLAESLVGIVSQRLVRRADGEGRVLATEILINTPLVQDYIKDEDKTPLIKEALLEDNIRGMQTFD
QHLMQLYRHHLITLEEALHHATSPHELRLMITRAGFLS

Sequences:

>Translated_358_residues
MTLDELLREMVGRRASDVHLQVGSPPMGRIDGSLLPFGTQPLTPSDTVALARALLTPEQWEEFEYRNELDLAYSVHGLGR
FRCNVFRQRGAVGIVMRTVNDAIPSFEALGLPADVLRGFAATSRGLLLITGPTSSGKSTTLAALIDHINRHFAYNILTIE
DPIEFLHRNHRSLVVQREVGSDTRDFRTALKYALRQDPDVIMIGEMRDRETVEAALSAAQTGHLVLSTLHTQDAVRSVNR
IIDFFPPHERTQVRLQLAESLVGIVSQRLVRRADGEGRVLATEILINTPLVQDYIKDEDKTPLIKEALLEDNIRGMQTFD
QHLMQLYRHHLITLEEALHHATSPHELRLMITRAGFLS
>Mature_357_residues
TLDELLREMVGRRASDVHLQVGSPPMGRIDGSLLPFGTQPLTPSDTVALARALLTPEQWEEFEYRNELDLAYSVHGLGRF
RCNVFRQRGAVGIVMRTVNDAIPSFEALGLPADVLRGFAATSRGLLLITGPTSSGKSTTLAALIDHINRHFAYNILTIED
PIEFLHRNHRSLVVQREVGSDTRDFRTALKYALRQDPDVIMIGEMRDRETVEAALSAAQTGHLVLSTLHTQDAVRSVNRI
IDFFPPHERTQVRLQLAESLVGIVSQRLVRRADGEGRVLATEILINTPLVQDYIKDEDKTPLIKEALLEDNIRGMQTFDQ
HLMQLYRHHLITLEEALHHATSPHELRLMITRAGFLS

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=40.7294832826748, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=34.5195729537367, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789723, Length=291, Percent_Identity=35.0515463917526, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40239; Mature: 40108

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDELLREMVGRRASDVHLQVGSPPMGRIDGSLLPFGTQPLTPSDTVALARALLTPEQW
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
EEFEYRNELDLAYSVHGLGRFRCNVFRQRGAVGIVMRTVNDAIPSFEALGLPADVLRGFA
HHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHCCCCHHHHHHHH
ATSRGLLLITGPTSSGKSTTLAALIDHINRHFAYNILTIEDPIEFLHRNHRSLVVQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHCCCHHEEEECCCC
SDTRDFRTALKYALRQDPDVIMIGEMRDRETVEAALSAAQTGHLVLSTLHTQDAVRSVNR
CCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IIDFFPPHERTQVRLQLAESLVGIVSQRLVRRADGEGRVLATEILINTPLVQDYIKDEDK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHCCCCC
TPLIKEALLEDNIRGMQTFDQHLMQLYRHHLITLEEALHHATSPHELRLMITRAGFLS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TLDELLREMVGRRASDVHLQVGSPPMGRIDGSLLPFGTQPLTPSDTVALARALLTPEQW
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
EEFEYRNELDLAYSVHGLGRFRCNVFRQRGAVGIVMRTVNDAIPSFEALGLPADVLRGFA
HHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHCCCCHHHHHHHH
ATSRGLLLITGPTSSGKSTTLAALIDHINRHFAYNILTIEDPIEFLHRNHRSLVVQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHCCCHHEEEECCCC
SDTRDFRTALKYALRQDPDVIMIGEMRDRETVEAALSAAQTGHLVLSTLHTQDAVRSVNR
CCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IIDFFPPHERTQVRLQLAESLVGIVSQRLVRRADGEGRVLATEILINTPLVQDYIKDEDK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHCCCCC
TPLIKEALLEDNIRGMQTFDQHLMQLYRHHLITLEEALHHATSPHELRLMITRAGFLS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]