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The map label for this gene is sppA [H]

Identifier: 94984761

GI number: 94984761

Start: 709574

End: 711184

Strand: Reverse

Name: sppA [H]

Synonym: Dgeo_0654

Alternate gene names: 94984761

Gene position: 711184-709574 (Counterclockwise)

Preceding gene: 94984762

Following gene: 94984753

Centisome position: 28.83

GC content: 68.22

Gene sequence:

>1611_bases
ATGACACCCTTCCACCTTCCCTTTCTCGGCCAGGACCGTGACAAGCTCCCGGACGGCGTGACCCGCCCCACTTGGGTGAT
CCTCGACGTGACCGGTTCCTATCCCGAGCGGCAGCCCAGCAACCCCATCCAGGCTCTCTTCAACCGCGAGGACACGCTGG
AAGGGCTGGCAGCTCGAGTGGACAAGCTGCGGCACGCGGACTGGCTGCACGGCGTCCTGGTCCGCATCTCCGAGTTCACA
GCGGCACCCGCGACCGCCCACGCCATCCGTCAGCTCCTCGCCCGACTGGCCCAAGACAAGCGGGTGGTGGCCTACCTGCC
ACAGCTCACCATGACGGCCCTGTTGGCCGCGAGCGGCGCGCGGGAGATCGTCGCGCCCGAGTCCGCCGATGTGCTGGTGC
CGGGGTTCGCCGTCCAGCCCACCTTTCTGGGTGCTTTCCTGAAAAGGCACGGCATCGAGTTTGAGAACCTGCGAATCCGG
GAATACAAGGCTGCCCTGACCCGCTTCTCGCAGGACCACATGGACGAGGCCAACCGCGAACAGCTCACCGCCTACTTGCA
CAGCCTAGAAAAGGCCTGGGCGCTCGACCTCGCAGCGGCGCGCGGCGTGAGCGAGGCGACAGCCCGCACGTGGCTGAGGG
GCGATCTGACGAGCGCCCGAGCCGCCCAGGCCGCGGGCCTCATCACTCAGGTGGCCTATGAGGATGAACTCGTCGGTCCC
GGTACGCGGCCTCTGGCGGCGGTGCTTGACCTGCTGCTGCCCCGGCGCGGCAACGCCAAAGCCGGGCGGGTTGCCGTCGT
CTCGCTGGTAGGCAGCATCGTGCCCGGCAAGAGCCGCCACAATCCCCTCCCCCTCCCGCTGCTGGGCGGCCCGCTGGCTG
GGTCGGACACCGTGGTCGCGGCCCTCAAACACGCCAAAAAGGACGACAAGACCAAGGCGATCGTGCTGTATGTGAACAGT
GGCGGCGGCTCGGCTCTGGCGTCTGACCTGATCTGGCGCGAGGTCGCCACCTCCGAAAAGCCGGTCGTCGCCGTGATGGG
CGAGTACGCGGCGAGCGGCGGCTACTACGTCCTCACGCACGCGCGGCACATCGTCGCCAGCCCCTATACCCTCACGGGCA
GCATCGGCGTGGTGAGCGGCAAGCCGGTCCTGCAGGAATTCAATGCCCGGCACGGCCTCAAGCCCGAACCCGTGGGGAGT
GAGCGCGCCCTGATGTACAGCGCCGCACGCCCCTTCAGCGACGAGGAACGCGCCCACGTCGAGCAGGGCATCGCGGAGGT
CTACGACCGCTTCGTGAACCGTGTGGCCGAGGGACGCGGCATGACGCCCGAGCGGGTGAACGAGATCGGACGGGGCCGCA
TCTGGAGCGGTTTGGATGCCCTCGACCTGGGGTTGGTCGATGAGCTGGGCGACCTCCGCACCGGGATCGAGCGCGCCTGC
GAACTCGCGGGCCTGCCCTACGACGCCCCAGTCTGGAATGCCACGCCCGCCAAAACCGGTCCCCTCCCTGAATTCGTGCA
GCAGGCCGCCCGCGCTGCCCAGGTGAGTGTGTGGCCTTTCGGACGTGAGCGGGTGCTGACGTGGCTCGATCAGGAGGTGA
ACGTGCGGTAA

Upstream 100 bases:

>100_bases
GGTCCGGAAGCGGCGGCGCCCCTCACTGCACGGGCAGAAGCGATGGGTCATCGGCTGGGCCAGAGAAACGCTCCTCACGT
CGGGCGCGTACCGTAGGGGT

Downstream 100 bases:

>100_bases
GCGGTCCCCGGAACGCGGTGGGTGGAGAGCGCTGGGGAGGCGCACCGTCTCCCCCCACGGACCGCGTCAAAGGTATTCCA
GGTCGCAGCAGGGCTGCGGC

Product: peptidase S49

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MTPFHLPFLGQDRDKLPDGVTRPTWVILDVTGSYPERQPSNPIQALFNREDTLEGLAARVDKLRHADWLHGVLVRISEFT
AAPATAHAIRQLLARLAQDKRVVAYLPQLTMTALLAASGAREIVAPESADVLVPGFAVQPTFLGAFLKRHGIEFENLRIR
EYKAALTRFSQDHMDEANREQLTAYLHSLEKAWALDLAAARGVSEATARTWLRGDLTSARAAQAAGLITQVAYEDELVGP
GTRPLAAVLDLLLPRRGNAKAGRVAVVSLVGSIVPGKSRHNPLPLPLLGGPLAGSDTVVAALKHAKKDDKTKAIVLYVNS
GGGSALASDLIWREVATSEKPVVAVMGEYAASGGYYVLTHARHIVASPYTLTGSIGVVSGKPVLQEFNARHGLKPEPVGS
ERALMYSAARPFSDEERAHVEQGIAEVYDRFVNRVAEGRGMTPERVNEIGRGRIWSGLDALDLGLVDELGDLRTGIERAC
ELAGLPYDAPVWNATPAKTGPLPEFVQQAARAAQVSVWPFGRERVLTWLDQEVNVR

Sequences:

>Translated_536_residues
MTPFHLPFLGQDRDKLPDGVTRPTWVILDVTGSYPERQPSNPIQALFNREDTLEGLAARVDKLRHADWLHGVLVRISEFT
AAPATAHAIRQLLARLAQDKRVVAYLPQLTMTALLAASGAREIVAPESADVLVPGFAVQPTFLGAFLKRHGIEFENLRIR
EYKAALTRFSQDHMDEANREQLTAYLHSLEKAWALDLAAARGVSEATARTWLRGDLTSARAAQAAGLITQVAYEDELVGP
GTRPLAAVLDLLLPRRGNAKAGRVAVVSLVGSIVPGKSRHNPLPLPLLGGPLAGSDTVVAALKHAKKDDKTKAIVLYVNS
GGGSALASDLIWREVATSEKPVVAVMGEYAASGGYYVLTHARHIVASPYTLTGSIGVVSGKPVLQEFNARHGLKPEPVGS
ERALMYSAARPFSDEERAHVEQGIAEVYDRFVNRVAEGRGMTPERVNEIGRGRIWSGLDALDLGLVDELGDLRTGIERAC
ELAGLPYDAPVWNATPAKTGPLPEFVQQAARAAQVSVWPFGRERVLTWLDQEVNVR
>Mature_535_residues
TPFHLPFLGQDRDKLPDGVTRPTWVILDVTGSYPERQPSNPIQALFNREDTLEGLAARVDKLRHADWLHGVLVRISEFTA
APATAHAIRQLLARLAQDKRVVAYLPQLTMTALLAASGAREIVAPESADVLVPGFAVQPTFLGAFLKRHGIEFENLRIRE
YKAALTRFSQDHMDEANREQLTAYLHSLEKAWALDLAAARGVSEATARTWLRGDLTSARAAQAAGLITQVAYEDELVGPG
TRPLAAVLDLLLPRRGNAKAGRVAVVSLVGSIVPGKSRHNPLPLPLLGGPLAGSDTVVAALKHAKKDDKTKAIVLYVNSG
GGSALASDLIWREVATSEKPVVAVMGEYAASGGYYVLTHARHIVASPYTLTGSIGVVSGKPVLQEFNARHGLKPEPVGSE
RALMYSAARPFSDEERAHVEQGIAEVYDRFVNRVAEGRGMTPERVNEIGRGRIWSGLDALDLGLVDELGDLRTGIERACE
LAGLPYDAPVWNATPAKTGPLPEFVQQAARAAQVSVWPFGRERVLTWLDQEVNVR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=463, Percent_Identity=28.0777537796976, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 58115; Mature: 57984

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFHLPFLGQDRDKLPDGVTRPTWVILDVTGSYPERQPSNPIQALFNREDTLEGLAARV
CCCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
DKLRHADWLHGVLVRISEFTAAPATAHAIRQLLARLAQDKRVVAYLPQLTMTALLAASGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
REIVAPESADVLVPGFAVQPTFLGAFLKRHGIEFENLRIREYKAALTRFSQDHMDEANRE
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCHHH
QLTAYLHSLEKAWALDLAAARGVSEATARTWLRGDLTSARAAQAAGLITQVAYEDELVGP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
GTRPLAAVLDLLLPRRGNAKAGRVAVVSLVGSIVPGKSRHNPLPLPLLGGPLAGSDTVVA
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHH
ALKHAKKDDKTKAIVLYVNSGGGSALASDLIWREVATSEKPVVAVMGEYAASGGYYVLTH
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCEEEEEC
ARHIVASPYTLTGSIGVVSGKPVLQEFNARHGLKPEPVGSERALMYSAARPFSDEERAHV
CHHHHCCCEEEECCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHH
EQGIAEVYDRFVNRVAEGRGMTPERVNEIGRGRIWSGLDALDLGLVDELGDLRTGIERAC
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAGLPYDAPVWNATPAKTGPLPEFVQQAARAAQVSVWPFGRERVLTWLDQEVNVR
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TPFHLPFLGQDRDKLPDGVTRPTWVILDVTGSYPERQPSNPIQALFNREDTLEGLAARV
CCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
DKLRHADWLHGVLVRISEFTAAPATAHAIRQLLARLAQDKRVVAYLPQLTMTALLAASGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
REIVAPESADVLVPGFAVQPTFLGAFLKRHGIEFENLRIREYKAALTRFSQDHMDEANRE
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCHHH
QLTAYLHSLEKAWALDLAAARGVSEATARTWLRGDLTSARAAQAAGLITQVAYEDELVGP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
GTRPLAAVLDLLLPRRGNAKAGRVAVVSLVGSIVPGKSRHNPLPLPLLGGPLAGSDTVVA
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHH
ALKHAKKDDKTKAIVLYVNSGGGSALASDLIWREVATSEKPVVAVMGEYAASGGYYVLTH
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCEEEEEC
ARHIVASPYTLTGSIGVVSGKPVLQEFNARHGLKPEPVGSERALMYSAARPFSDEERAHV
CHHHHCCCEEEECCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHH
EQGIAEVYDRFVNRVAEGRGMTPERVNEIGRGRIWSGLDALDLGLVDELGDLRTGIERAC
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAGLPYDAPVWNATPAKTGPLPEFVQQAARAAQVSVWPFGRERVLTWLDQEVNVR
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]