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The map label for this gene is purR [H]

Identifier: 94984687

GI number: 94984687

Start: 618368

End: 619360

Strand: Reverse

Name: purR [H]

Synonym: Dgeo_0580

Alternate gene names: 94984687

Gene position: 619360-618368 (Counterclockwise)

Preceding gene: 94984691

Following gene: 94984677

Centisome position: 25.1

GC content: 67.37

Gene sequence:

>993_bases
ATGCGCAAACCCACGATTCAGGATGTCGCCCGGCGAGCCGGCGTAGGCGTCGGCACCGTTTCCCGCGTGCTGAATAACCA
CGCCGCGGTCAAGGGCGCGACCCGCGAGACGGTCCTGAAGGCCATTGCGGACCTCAACTACACGCCAAACCCGCATGCCC
GGCGGATCGCGGGCGGCAAGAGCTATACGATCAGTGTGCTGCTCCCGGTCGTGACCACCGAGTTCTATGTGCGCCTGCTG
GACGGCCTGGAAAGCGCCTTTCAGGAGGCCCGCTACGACGTGGCGATCTTTCCGCTGCTCGACCGCTCACGGCTGGAACG
CTACCTGGCATCACATACCCTGGCCTATCAGGCCGACGGGCTGGTGATGGCGACCTACAACCTCACCCAACTGTTTAACG
AGCGTCGCCTGCGCAATCAGCAACCGACCGTCCTGGTCGATGCCTACGGCGAGGGCGTGGACTGTGCCTACATGGACAAC
GTGACCGGCGGGCGGCTGGCGGGCGAGTATGCGGTCACGCTGCCCGGCGAGCTGTACGCGATCTGGGTGGAGACCGAGCT
CGACCAGCTCTTCACCACCCGCGTGTTCGAGGAGCGCCGGTCGGGCTTTCATCAGGCGGTCCAGGCGGCGGGCCGAGCCG
TGCACGCCGAATTCACCGCCAGCTTCGACGCGCTGGCGGCCCGCAACGCCGCCGTCACCTTGCTCGCTGAAGCCCAGTTT
CCGTGCACGGTCTTCGCCTCCGCCGACCTGCTGGCAGGTGCCGTACTGGACGAGGCACAGCTGCGCGGCCTGATTCCCGG
CCAGGACGTGCGGGTGATTGGCTTTGACGACCAGCCCTGGGCAGCCTCGCGCGGCCTCACCACCCTGCATCAGCCGGTCG
AGCAGATGGGGTACGAGGCAGCTCAACTCCTTTTGACCCGCCTAGGGGGATACCGCGGCCCGCCCCGCGCTCGCCGCTTC
GAACCCCGCCTGGTCGTGCGCGACACCGCCTGA

Upstream 100 bases:

>100_bases
CGCAGTCTAGGCGCAGGGGCAGGGCCGAATTGTCCCCCCTTCCCAGCCCGGCGCCAGTGGAACGGGTTCCAAACGTCGGG
GTATGGTGGAGGTGAGAGCT

Downstream 100 bases:

>100_bases
GGCGGCAGGAGTTTCCCCTCAGCCGCGCCGCCCCACCGGCTCCAGCGGGGTGTCGTGATGGGTGCGGCGCACGTCGGCCA
GGTGAACATCATTGCGGCCC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRKPTIQDVARRAGVGVGTVSRVLNNHAAVKGATRETVLKAIADLNYTPNPHARRIAGGKSYTISVLLPVVTTEFYVRLL
DGLESAFQEARYDVAIFPLLDRSRLERYLASHTLAYQADGLVMATYNLTQLFNERRLRNQQPTVLVDAYGEGVDCAYMDN
VTGGRLAGEYAVTLPGELYAIWVETELDQLFTTRVFEERRSGFHQAVQAAGRAVHAEFTASFDALAARNAAVTLLAEAQF
PCTVFASADLLAGAVLDEAQLRGLIPGQDVRVIGFDDQPWAASRGLTTLHQPVEQMGYEAAQLLLTRLGGYRGPPRARRF
EPRLVVRDTA

Sequences:

>Translated_330_residues
MRKPTIQDVARRAGVGVGTVSRVLNNHAAVKGATRETVLKAIADLNYTPNPHARRIAGGKSYTISVLLPVVTTEFYVRLL
DGLESAFQEARYDVAIFPLLDRSRLERYLASHTLAYQADGLVMATYNLTQLFNERRLRNQQPTVLVDAYGEGVDCAYMDN
VTGGRLAGEYAVTLPGELYAIWVETELDQLFTTRVFEERRSGFHQAVQAAGRAVHAEFTASFDALAARNAAVTLLAEAQF
PCTVFASADLLAGAVLDEAQLRGLIPGQDVRVIGFDDQPWAASRGLTTLHQPVEQMGYEAAQLLLTRLGGYRGPPRARRF
EPRLVVRDTA
>Mature_330_residues
MRKPTIQDVARRAGVGVGTVSRVLNNHAAVKGATRETVLKAIADLNYTPNPHARRIAGGKSYTISVLLPVVTTEFYVRLL
DGLESAFQEARYDVAIFPLLDRSRLERYLASHTLAYQADGLVMATYNLTQLFNERRLRNQQPTVLVDAYGEGVDCAYMDN
VTGGRLAGEYAVTLPGELYAIWVETELDQLFTTRVFEERRSGFHQAVQAAGRAVHAEFTASFDALAARNAAVTLLAEAQF
PCTVFASADLLAGAVLDEAQLRGLIPGQDVRVIGFDDQPWAASRGLTTLHQPVEQMGYEAAQLLLTRLGGYRGPPRARRF
EPRLVVRDTA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=28.2674772036474, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=31.1111111111111, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=26.8221574344023, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=28.1437125748503, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI48994940, Length=335, Percent_Identity=27.7611940298507, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=25.5255255255255, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786540, Length=322, Percent_Identity=25.776397515528, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789202, Length=340, Percent_Identity=25.2941176470588, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790192, Length=222, Percent_Identity=27.9279279279279, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786268, Length=280, Percent_Identity=26.4285714285714, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790715, Length=121, Percent_Identity=30.5785123966942, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36282; Mature: 36282

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPTIQDVARRAGVGVGTVSRVLNNHAAVKGATRETVLKAIADLNYTPNPHARRIAGGK
CCCCCHHHHHHHCCCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCCCC
SYTISVLLPVVTTEFYVRLLDGLESAFQEARYDVAIFPLLDRSRLERYLASHTLAYQADG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHEEECCC
LVMATYNLTQLFNERRLRNQQPTVLVDAYGEGVDCAYMDNVTGGRLAGEYAVTLPGELYA
EEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEECCCCEEE
IWVETELDQLFTTRVFEERRSGFHQAVQAAGRAVHAEFTASFDALAARNAAVTLLAEAQF
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCEEEEEECCCC
PCTVFASADLLAGAVLDEAQLRGLIPGQDVRVIGFDDQPWAASRGLTTLHQPVEQMGYEA
CEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHHHH
AQLLLTRLGGYRGPPRARRFEPRLVVRDTA
HHHHHHHHCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRKPTIQDVARRAGVGVGTVSRVLNNHAAVKGATRETVLKAIADLNYTPNPHARRIAGGK
CCCCCHHHHHHHCCCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCCCC
SYTISVLLPVVTTEFYVRLLDGLESAFQEARYDVAIFPLLDRSRLERYLASHTLAYQADG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHEEECCC
LVMATYNLTQLFNERRLRNQQPTVLVDAYGEGVDCAYMDNVTGGRLAGEYAVTLPGELYA
EEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEECCCCEEE
IWVETELDQLFTTRVFEERRSGFHQAVQAAGRAVHAEFTASFDALAARNAAVTLLAEAQF
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCEEEEEECCCC
PCTVFASADLLAGAVLDEAQLRGLIPGQDVRVIGFDDQPWAASRGLTTLHQPVEQMGYEA
CEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHHHH
AQLLLTRLGGYRGPPRARRFEPRLVVRDTA
HHHHHHHHCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA