Click here to switch to the map view.

The map label for this gene is proS

Identifier: 94984677

GI number: 94984677

Start: 609253

End: 610746

Strand: Reverse

Name: proS

Synonym: Dgeo_0570

Alternate gene names: 94984677

Gene position: 610746-609253 (Counterclockwise)

Preceding gene: 94984687

Following gene: 94984676

Centisome position: 24.75

GC content: 62.99

Gene sequence:

>1494_bases
ATGCCCCCTATGACGAAGGCCGAGGGCAAGCAGGACAAGAAGGCGCAGCAGTACGGGGTGACGCCTCAGAGCGTGGATTT
CAACGACTGGTACAACGAGGTGGTCAAGAAGGCCGATCTGGCCGATAACAGCCCCGTCGCGGGCGCGATGGTGGTCAAGC
CCTACGGCACCGCCCTCTGGGAGAACATCCAGCGCTGGCTGGACGACCGCTTCAAGGCGACCGGCCACGAGAGCCTGATC
TTCCCCACCCTGATCCCGATGAACTTCATCACCAAAGAAGCCGATCACGTGGAGGGCTTTGCCCCCGAACTGTTCACGGT
GGACCGCATCGGCACCGAGCAACTGACCGAGCCGTACGTGCTGCGGCCCACCTCCGAAACAATCATCGGCTACATGTGGA
GCGGGTGGCTCAACTCCTACCGTGACCTTCCCTTCCTGCACTATCAGTGGGGCAGCGTATTCCGCGCGGAACTGCGGACG
AAGGCCTTCTTGCGCACCTCTGAGTTCTATTGGCACGAGGGCCACACCGCCCACGCCTCCGAAGAAGAAGCCCGGCGCGA
GGTCCGCCAGATCCTCGACCTCTACCACGAGTTCTGCCGCGACATTCTCGCGCTGCCCGTCGTGCGCGGTGAGAAGACGG
CCAGCGAGCGATTCGCCGGGGCGGTCGCTACCTACTCTATCGAGGGCATGATGCGCGACGGCAAGGCGCTGCAATCAGGC
ACCTCGCATTACCTGGGGCAGAATTTCTCCAAGGCCTTTGACGTGAAGTTCCAGACGCGCGAGCAGCGTGAGGAGTACGC
CTACACCACGAGCTGGGCGATCTCCAGCCGCATCATCGGCGCAATCATCATGACGCACGGGGACGACTTCGGCCTGATCA
TGCCGCCCCGCATCGCGCCCATCCAGGTGGTCGTGATTCCGGTGAGCCGCAAGGAGAACTTCGATCAGATGGTGGCGGAG
GGCGAGAAGCTGGCCCATGAACTGCGCGCGCAGGGCCTCCGCGTGAAGGTGGACCGGCGCGAAGGCGTCACCAACGGTTT
CAAGTACAACGACTGGGAACTCAAGGGGGTGCCTGTCCGCATCGAGCTTGGCCCGCGCGATCTGGAGCAGGGCGTCGTGG
TGGTTAAAAACCGCAACGCCGAGGAGAAGGAGACGCTGCCGCGCGAAGAGGCAATCCGCGGCATGGCCAACCGCCTGGAC
AGCATCCACAATTGGCTGCTGCAGCGCGCAACGGACTTCCTGCTGACACATACCGTCCCCGCTGACAGCTACGAGGAGCT
GAAGAACGCCATCGAGCACGGCAACTGGGTGCGGGCCTTCCACTGCGGAAACGCCGAATGCGAGGCCCAAATCAAAGAGG
ACACCAAGGCCACCACCCGCAATATTCCTCTCGACGACGCCGAGTTCTTCAATGAGCGGGAGGAGGGCGTGTGCGTGAAG
TGCGGTCAGCCGAGCGCGTACGGCAAGCGGGTGATTTTCGGGCGGCAGTACTGA

Upstream 100 bases:

>100_bases
CCGTTAGGCAACATTGGGAGAATGTCCATGTCGGCCTGGCCACGCGGCCTAGGCCAAACCCACCCGGCGGCAGATTCCGC
CCCCACCCGGCCCCGCTATC

Downstream 100 bases:

>100_bases
GCAAGGCCCGGACTGAGCAAGGTCCGGGTCGGAGCGGCCCCCCTCCCTTTATCCTGGCCCGGTGACCCCTCCGCGCCAGC
TGCCGCCGTCCGCCGCCTTC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPPMTKAEGKQDKKAQQYGVTPQSVDFNDWYNEVVKKADLADNSPVAGAMVVKPYGTALWENIQRWLDDRFKATGHESLI
FPTLIPMNFITKEADHVEGFAPELFTVDRIGTEQLTEPYVLRPTSETIIGYMWSGWLNSYRDLPFLHYQWGSVFRAELRT
KAFLRTSEFYWHEGHTAHASEEEARREVRQILDLYHEFCRDILALPVVRGEKTASERFAGAVATYSIEGMMRDGKALQSG
TSHYLGQNFSKAFDVKFQTREQREEYAYTTSWAISSRIIGAIIMTHGDDFGLIMPPRIAPIQVVVIPVSRKENFDQMVAE
GEKLAHELRAQGLRVKVDRREGVTNGFKYNDWELKGVPVRIELGPRDLEQGVVVVKNRNAEEKETLPREEAIRGMANRLD
SIHNWLLQRATDFLLTHTVPADSYEELKNAIEHGNWVRAFHCGNAECEAQIKEDTKATTRNIPLDDAEFFNEREEGVCVK
CGQPSAYGKRVIFGRQY

Sequences:

>Translated_497_residues
MPPMTKAEGKQDKKAQQYGVTPQSVDFNDWYNEVVKKADLADNSPVAGAMVVKPYGTALWENIQRWLDDRFKATGHESLI
FPTLIPMNFITKEADHVEGFAPELFTVDRIGTEQLTEPYVLRPTSETIIGYMWSGWLNSYRDLPFLHYQWGSVFRAELRT
KAFLRTSEFYWHEGHTAHASEEEARREVRQILDLYHEFCRDILALPVVRGEKTASERFAGAVATYSIEGMMRDGKALQSG
TSHYLGQNFSKAFDVKFQTREQREEYAYTTSWAISSRIIGAIIMTHGDDFGLIMPPRIAPIQVVVIPVSRKENFDQMVAE
GEKLAHELRAQGLRVKVDRREGVTNGFKYNDWELKGVPVRIELGPRDLEQGVVVVKNRNAEEKETLPREEAIRGMANRLD
SIHNWLLQRATDFLLTHTVPADSYEELKNAIEHGNWVRAFHCGNAECEAQIKEDTKATTRNIPLDDAEFFNEREEGVCVK
CGQPSAYGKRVIFGRQY
>Mature_496_residues
PPMTKAEGKQDKKAQQYGVTPQSVDFNDWYNEVVKKADLADNSPVAGAMVVKPYGTALWENIQRWLDDRFKATGHESLIF
PTLIPMNFITKEADHVEGFAPELFTVDRIGTEQLTEPYVLRPTSETIIGYMWSGWLNSYRDLPFLHYQWGSVFRAELRTK
AFLRTSEFYWHEGHTAHASEEEARREVRQILDLYHEFCRDILALPVVRGEKTASERFAGAVATYSIEGMMRDGKALQSGT
SHYLGQNFSKAFDVKFQTREQREEYAYTTSWAISSRIIGAIIMTHGDDFGLIMPPRIAPIQVVVIPVSRKENFDQMVAEG
EKLAHELRAQGLRVKVDRREGVTNGFKYNDWELKGVPVRIELGPRDLEQGVVVVKNRNAEEKETLPREEAIRGMANRLDS
IHNWLLQRATDFLLTHTVPADSYEELKNAIEHGNWVRAFHCGNAECEAQIKEDTKATTRNIPLDDAEFFNEREEGVCVKC
GQPSAYGKRVIFGRQY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=495, Percent_Identity=37.7777777777778, Blast_Score=352, Evalue=4e-97,
Organism=Escherichia coli, GI1786392, Length=160, Percent_Identity=30.625, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71991342, Length=478, Percent_Identity=38.4937238493724, Blast_Score=347, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI71991346, Length=364, Percent_Identity=42.5824175824176, Blast_Score=314, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6321808, Length=512, Percent_Identity=37.3046875, Blast_Score=353, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24649468, Length=496, Percent_Identity=35.8870967741936, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24649466, Length=496, Percent_Identity=35.8870967741936, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_DEIGD (Q1J0W2)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_604041.1
- ProteinModelPortal:   Q1J0W2
- SMR:   Q1J0W2
- STRING:   Q1J0W2
- GeneID:   4058581
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0570
- NMPDR:   fig|68909.1.peg.872
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- PhylomeDB:   Q1J0W2
- ProtClustDB:   PRK08661
- BioCyc:   DGEO319795:DGEO_0570-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 56862; Mature: 56731

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPMTKAEGKQDKKAQQYGVTPQSVDFNDWYNEVVKKADLADNSPVAGAMVVKPYGTALW
CCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHH
ENIQRWLDDRFKATGHESLIFPTLIPMNFITKEADHVEGFAPELFTVDRIGTEQLTEPYV
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCEEECCCCHHHCCCCEE
LRPTSETIIGYMWSGWLNSYRDLPFLHYQWGSVFRAELRTKAFLRTSEFYWHEGHTAHAS
ECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
EEEARREVRQILDLYHEFCRDILALPVVRGEKTASERFAGAVATYSIEGMMRDGKALQSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEHHHHHCCHHHHCC
TSHYLGQNFSKAFDVKFQTREQREEYAYTTSWAISSRIIGAIIMTHGDDFGLIMPPRIAP
CHHHHCCCCCHHCCEEECCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCCCCC
IQVVVIPVSRKENFDQMVAEGEKLAHELRAQGLRVKVDRREGVTNGFKYNDWELKGVPVR
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECEEEE
IELGPRDLEQGVVVVKNRNAEEKETLPREEAIRGMANRLDSIHNWLLQRATDFLLTHTVP
EEECCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
ADSYEELKNAIEHGNWVRAFHCGNAECEAQIKEDTKATTRNIPLDDAEFFNEREEGVCVK
CCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCHHHHCCCCCCCHHHHCCCCCCEEEE
CGQPSAYGKRVIFGRQY
CCCCCCCCCEEEECCCC
>Mature Secondary Structure 
PPMTKAEGKQDKKAQQYGVTPQSVDFNDWYNEVVKKADLADNSPVAGAMVVKPYGTALW
CCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHH
ENIQRWLDDRFKATGHESLIFPTLIPMNFITKEADHVEGFAPELFTVDRIGTEQLTEPYV
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCEEECCCCHHHCCCCEE
LRPTSETIIGYMWSGWLNSYRDLPFLHYQWGSVFRAELRTKAFLRTSEFYWHEGHTAHAS
ECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
EEEARREVRQILDLYHEFCRDILALPVVRGEKTASERFAGAVATYSIEGMMRDGKALQSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEHHHHHCCHHHHCC
TSHYLGQNFSKAFDVKFQTREQREEYAYTTSWAISSRIIGAIIMTHGDDFGLIMPPRIAP
CHHHHCCCCCHHCCEEECCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCCCCC
IQVVVIPVSRKENFDQMVAEGEKLAHELRAQGLRVKVDRREGVTNGFKYNDWELKGVPVR
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECEEEE
IELGPRDLEQGVVVVKNRNAEEKETLPREEAIRGMANRLDSIHNWLLQRATDFLLTHTVP
EEECCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
ADSYEELKNAIEHGNWVRAFHCGNAECEAQIKEDTKATTRNIPLDDAEFFNEREEGVCVK
CCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCHHHHCCCCCCCHHHHCCCCCCEEEE
CGQPSAYGKRVIFGRQY
CCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA