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The map label for this gene is trmE

Identifier: 94984585

GI number: 94984585

Start: 493774

End: 495093

Strand: Reverse

Name: trmE

Synonym: Dgeo_0478

Alternate gene names: 94984585

Gene position: 495093-493774 (Counterclockwise)

Preceding gene: 94984587

Following gene: 94984581

Centisome position: 20.07

GC content: 70.38

Gene sequence:

>1320_bases
GTGACCCGCACCGGCCTCTCGGATACCATCGCCGCCATTGCAACCGCGCCGGGGAGCGCGGGGGTGGGCATCGTACGCAT
CAGCGGCCCGGACGCGCTGGGGATAGCAGACGGCGCGTTCCGGGGAAGGCGCAGACCCAGCGCCACACGTGGGGGCCGCT
TCTTGTTCGGGCAACTGGTCGCTGGAGACGGCGAGGTGCTGGACGAGGGCCTCTGCCTGGTGTTCCGCGGGCCGCACAGC
TACACCGGCGAGGATGTGGCCGAGTTGCAAACGCACGGCAGCCCGGCGGTGCTGGCCCGCGTGCTCTCGCGAGTCCTGGA
ACTCGGCGCGCGGCCCGCCCGACCCGGTGAGTTCACCCTGCGGGCGTACCTAGCGGGCAGGCTTGACCTCGCTCAGGCGG
AGGCGGTGCTGGAACTGGTGAATGCGGGCACCGAGACGGCGCGGCGGCAGGCGGCGCTGGGCCTCTCGGGCGCGCTGGGG
GAACGGGTCGAGCGTATTGCCGCCCACGTCACGCGGACGCTGGCTGCCCTGCAGGCGATGCTCGACTATCCCGAGGAAGG
CGTGCCGGACGAAGACCGCACCGTTCCTCTCGCGGCGGCGGAGGCGGAGCTGCACGCACTCGTGGGCACGGCGCGCGCGG
GTCAGGTGGCCACCCGAGGTGCGCGCCTTGCCCTGATCGGCCGCCCAAATGCCGGCAAGAGCAGCCTGCTCAATGCCCTG
CTGGGCTACGAGCGTTCCATCGTGACACCCATTCCCGGCACCACCCGCGACTATCTCGAAGCCCAACTCTCGCTGGCGGG
CGTGCCGGTCACCCTGGTAGACACCGCCGGCCTCCGCGAGACGGGTGATGAGGTAGAAGCCGCAGGCGTGCGGCAGGCGG
TGCGTCTTGCCGAATCAGCGGATCTGGTGCTTGTCCTGGAAGATGGCAGCCAGCCGCGCGATCACCTGCCCGCCGAACTG
CCGAGAGAGACGCGCATGCTTCGGGTGCGGACCAAAGCGGACCTCCCCGCTGCCTGGACTGATCCGGGCGCACTGGACGT
GAGCGCCGTGACCGGGCAGGGCCTCTCCGCCCTGCGGGACGCGATTCACACCGCCCTGATCGGCGACGCTGCCCAGGGTG
AGGCGTGGTTGACCACCGAGCGGCAGGCGGACGCGGCACGGCGGGCACTCACCCACATTCAGGCGGCCCGCAGCCTCCCC
GACGATCTGGCCGGGTACGAGCTGGAAGAAGCGTTGCGGGCGCTGGCCGACCTCACCGGACGCGATGTCCAAGATGATGT
GGTGGACGCGGTATTCCGCAATTTTTGTGTGGGAAAGTGA

Upstream 100 bases:

>100_bases
AACAAATACTGTTCATCAAAGCGGGCTGACCAGGGCGAACATCCTCACCCTGGTCAGCCTCCCTCTTTTTTCTCCTAGCT
CGCCTGCTACCCTGCTCGCC

Downstream 100 bases:

>100_bases
GGGGCTAACAGGGTTCCTCCCACCGACCCTCAGGCCCGCTTCAGACCTCGTCGTCCTTGGTGCTCAGAGACGTTTCCTCA
CCCGGGGTGGTGCTGGCCGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTRTGLSDTIAAIATAPGSAGVGIVRISGPDALGIADGAFRGRRRPSATRGGRFLFGQLVAGDGEVLDEGLCLVFRGPHS
YTGEDVAELQTHGSPAVLARVLSRVLELGARPARPGEFTLRAYLAGRLDLAQAEAVLELVNAGTETARRQAALGLSGALG
ERVERIAAHVTRTLAALQAMLDYPEEGVPDEDRTVPLAAAEAELHALVGTARAGQVATRGARLALIGRPNAGKSSLLNAL
LGYERSIVTPIPGTTRDYLEAQLSLAGVPVTLVDTAGLRETGDEVEAAGVRQAVRLAESADLVLVLEDGSQPRDHLPAEL
PRETRMLRVRTKADLPAAWTDPGALDVSAVTGQGLSALRDAIHTALIGDAAQGEAWLTTERQADAARRALTHIQAARSLP
DDLAGYELEEALRALADLTGRDVQDDVVDAVFRNFCVGK

Sequences:

>Translated_439_residues
MTRTGLSDTIAAIATAPGSAGVGIVRISGPDALGIADGAFRGRRRPSATRGGRFLFGQLVAGDGEVLDEGLCLVFRGPHS
YTGEDVAELQTHGSPAVLARVLSRVLELGARPARPGEFTLRAYLAGRLDLAQAEAVLELVNAGTETARRQAALGLSGALG
ERVERIAAHVTRTLAALQAMLDYPEEGVPDEDRTVPLAAAEAELHALVGTARAGQVATRGARLALIGRPNAGKSSLLNAL
LGYERSIVTPIPGTTRDYLEAQLSLAGVPVTLVDTAGLRETGDEVEAAGVRQAVRLAESADLVLVLEDGSQPRDHLPAEL
PRETRMLRVRTKADLPAAWTDPGALDVSAVTGQGLSALRDAIHTALIGDAAQGEAWLTTERQADAARRALTHIQAARSLP
DDLAGYELEEALRALADLTGRDVQDDVVDAVFRNFCVGK
>Mature_438_residues
TRTGLSDTIAAIATAPGSAGVGIVRISGPDALGIADGAFRGRRRPSATRGGRFLFGQLVAGDGEVLDEGLCLVFRGPHSY
TGEDVAELQTHGSPAVLARVLSRVLELGARPARPGEFTLRAYLAGRLDLAQAEAVLELVNAGTETARRQAALGLSGALGE
RVERIAAHVTRTLAALQAMLDYPEEGVPDEDRTVPLAAAEAELHALVGTARAGQVATRGARLALIGRPNAGKSSLLNALL
GYERSIVTPIPGTTRDYLEAQLSLAGVPVTLVDTAGLRETGDEVEAAGVRQAVRLAESADLVLVLEDGSQPRDHLPAELP
RETRMLRVRTKADLPAAWTDPGALDVSAVTGQGLSALRDAIHTALIGDAAQGEAWLTTERQADAARRALTHIQAARSLPD
DLAGYELEEALRALADLTGRDVQDDVVDAVFRNFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=36.989247311828, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI193082993, Length=451, Percent_Identity=36.5853658536585, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=34.8790322580645, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=38.8157894736842, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082120, Length=93, Percent_Identity=40.8602150537634, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=448, Percent_Identity=30.1339285714286, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=27.8118609406953, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=30.4801670146138, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_DEIGD (Q1J154)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603949.1
- ProteinModelPortal:   Q1J154
- SMR:   Q1J154
- STRING:   Q1J154
- GeneID:   4057909
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0478
- NMPDR:   fig|68909.1.peg.778
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q1J154
- ProtClustDB:   PRK05291
- BioCyc:   DGEO319795:DGEO_0478-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 46026; Mature: 45894

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 88-88 BINDING 127-127 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTGLSDTIAAIATAPGSAGVGIVRISGPDALGIADGAFRGRRRPSATRGGRFLFGQLV
CCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEE
AGDGEVLDEGLCLVFRGPHSYTGEDVAELQTHGSPAVLARVLSRVLELGARPARPGEFTL
CCCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
RAYLAGRLDLAQAEAVLELVNAGTETARRQAALGLSGALGERVERIAAHVTRTLAALQAM
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDYPEEGVPDEDRTVPLAAAEAELHALVGTARAGQVATRGARLALIGRPNAGKSSLLNAL
HCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCHHHHHHHH
LGYERSIVTPIPGTTRDYLEAQLSLAGVPVTLVDTAGLRETGDEVEAAGVRQAVRLAESA
HCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
DLVLVLEDGSQPRDHLPAELPRETRMLRVRTKADLPAAWTDPGALDVSAVTGQGLSALRD
CEEEEECCCCCCHHCCCCCCCCHHHEEEEEECCCCCCCCCCCCCEEEEHHCCCCHHHHHH
AIHTALIGDAAQGEAWLTTERQADAARRALTHIQAARSLPDDLAGYELEEALRALADLTG
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCC
RDVQDDVVDAVFRNFCVGK
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRTGLSDTIAAIATAPGSAGVGIVRISGPDALGIADGAFRGRRRPSATRGGRFLFGQLV
CCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEE
AGDGEVLDEGLCLVFRGPHSYTGEDVAELQTHGSPAVLARVLSRVLELGARPARPGEFTL
CCCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
RAYLAGRLDLAQAEAVLELVNAGTETARRQAALGLSGALGERVERIAAHVTRTLAALQAM
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDYPEEGVPDEDRTVPLAAAEAELHALVGTARAGQVATRGARLALIGRPNAGKSSLLNAL
HCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCHHHHHHHH
LGYERSIVTPIPGTTRDYLEAQLSLAGVPVTLVDTAGLRETGDEVEAAGVRQAVRLAESA
HCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
DLVLVLEDGSQPRDHLPAELPRETRMLRVRTKADLPAAWTDPGALDVSAVTGQGLSALRD
CEEEEECCCCCCHHCCCCCCCCHHHEEEEEECCCCCCCCCCCCCEEEEHHCCCCHHHHHH
AIHTALIGDAAQGEAWLTTERQADAARRALTHIQAARSLPDDLAGYELEEALRALADLTG
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCC
RDVQDDVVDAVFRNFCVGK
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA