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The map label for this gene is rimO

Identifier: 94984483

GI number: 94984483

Start: 379956

End: 381413

Strand: Reverse

Name: rimO

Synonym: Dgeo_0375

Alternate gene names: 94984483

Gene position: 381413-379956 (Counterclockwise)

Preceding gene: 94984488

Following gene: 94984482

Centisome position: 15.46

GC content: 64.2

Gene sequence:

>1458_bases
ATGACGAGGACACAAGAACCGCTGCCGACCGTGGCAGCCCCCAAAGTCGGCTTTATCAGTCTCGGCTGCCCCAAGGCGCT
CGTGGACAGCGAGCGTATTCTGACCCAACTGCGCGCCGAGGGTTACGAGGTCGCGCCCAACTACGAGGATGCCCAGGCGG
TGATTGTCAATACCTGTGGCTTCATCACGCCCGCCGTCGAGGAGTCGCTGAGCGCGATTGGCGAGGCGCTCGACGCCACC
GGCAAGGTGATTGTGACCGGCTGCCTGGGTGAGCGGCCCGAAAAGATTCTCGAGCGTCACCCCAAAGTCGCGGCGATCAC
CGGCTCAGAAGCGGTGGATGACGTGATGGCGCATGTACGTGAGCTGCTGCCAATCGAGCTGGACCCCTTCACGGGGCTGC
TGCCGGTGGCCGCGCCCGGCATGCGCCAGGGGGACACGCTCGCGCCCAGCGTCAAGCTCACGCCCCGGCACTACGCCTAT
GTAAAGATCGCGGAGGGCTGCAACCACACCTGCTCGTTTTGCATCATCCCCAAGCTGCGTGGGCGGCAGGTCAGCCGCGA
CGCGGGTGCTGTGCTGTACGAGGCCTACCGGCTGATTGCGGGCGGCACCAAAGAACTGATGATCATCTCGCAGGACACGT
CGGCCTACGGGGTAGACCTCCGGCACCGCACATCGGAATTTCAGGGCGAGCAGGTGCGCGCCCATCTCATTGACCTGGCC
GAGAAACTGGGCGAGATGGGGGCTTGGGTGCGGATGCACTACGTCTATCCCTACCCGCATGTCGAGCGAATTGTGGAACT
CATGAGCCAGGGCAAGATTCTGCCCTATCTCGACGTGCCGCTCCAGCACGCCTCGCCCGCCGTCCTCAAGCGGATGCGGC
GGCCCGGTGCAGGCAAGCAACTCGACACCATCCGGCGCTGGCGCGAGATTTGCCCCGAACTCGTCATCCGTTCCACCTTC
ATTGTGGGCTTTCCCGGCGAGACCGAGGAGGACTTTCAGCTGCTCCTCGACTTTCTAGAGGAGGCGCGGCTTGACCGCGT
GGGCGCCTTCACCTATTCGGATGTGGAGGAGGCGGATGCCAACGCCCTCGACGGCGCCATCCCCGAGGAGGTTAAGCAGG
AGCGGCTCGCGCGCTTCATGGAAGTCGCCCAGCGCATCAGCCGGGAGAAGCTCGCCGAGAAGGTGGGCCGCGTGCTGGAC
GTGATCATCGACGAGTTCAATGACGATGAGGGGGACGAGCCGGGCACCCGCCTGATCGGGCGAACCAAGGGCGACGCCCC
CGGCATCGACGGCCAAGTCTACCTGTACGCGGGCGACTTTGCGGGGCAAGTCAAAATCGGCGATATCGTTCAGGCACGCA
TCGAGGACAGTGACGAGTACGACCTCTACGGCGAAGTGATCCACACACCCGAGTGGCGGCCCAACGTGCCCTTGCTGGGT
CACTTCGGAAAACACTGA

Upstream 100 bases:

>100_bases
CGTATCCTATCCGCCCCCACTCCCACCCTGTACACTGCTTGGCCGAGTCTCGCGCCCGGCGTCTCCCCACGCGGCGGCGC
GTCCGTGTGGAGGAAGAGAG

Downstream 100 bases:

>100_bases
GGGGCAACACGGTTCCAGGGGGCGCGCAAGCTCCCTGTCAGAACGAACGGCCTAGACTCTGCGGGTGAGCCTTGACGTTC
TGCTCGTGGTCTTCGCCGGC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTRTQEPLPTVAAPKVGFISLGCPKALVDSERILTQLRAEGYEVAPNYEDAQAVIVNTCGFITPAVEESLSAIGEALDAT
GKVIVTGCLGERPEKILERHPKVAAITGSEAVDDVMAHVRELLPIELDPFTGLLPVAAPGMRQGDTLAPSVKLTPRHYAY
VKIAEGCNHTCSFCIIPKLRGRQVSRDAGAVLYEAYRLIAGGTKELMIISQDTSAYGVDLRHRTSEFQGEQVRAHLIDLA
EKLGEMGAWVRMHYVYPYPHVERIVELMSQGKILPYLDVPLQHASPAVLKRMRRPGAGKQLDTIRRWREICPELVIRSTF
IVGFPGETEEDFQLLLDFLEEARLDRVGAFTYSDVEEADANALDGAIPEEVKQERLARFMEVAQRISREKLAEKVGRVLD
VIIDEFNDDEGDEPGTRLIGRTKGDAPGIDGQVYLYAGDFAGQVKIGDIVQARIEDSDEYDLYGEVIHTPEWRPNVPLLG
HFGKH

Sequences:

>Translated_485_residues
MTRTQEPLPTVAAPKVGFISLGCPKALVDSERILTQLRAEGYEVAPNYEDAQAVIVNTCGFITPAVEESLSAIGEALDAT
GKVIVTGCLGERPEKILERHPKVAAITGSEAVDDVMAHVRELLPIELDPFTGLLPVAAPGMRQGDTLAPSVKLTPRHYAY
VKIAEGCNHTCSFCIIPKLRGRQVSRDAGAVLYEAYRLIAGGTKELMIISQDTSAYGVDLRHRTSEFQGEQVRAHLIDLA
EKLGEMGAWVRMHYVYPYPHVERIVELMSQGKILPYLDVPLQHASPAVLKRMRRPGAGKQLDTIRRWREICPELVIRSTF
IVGFPGETEEDFQLLLDFLEEARLDRVGAFTYSDVEEADANALDGAIPEEVKQERLARFMEVAQRISREKLAEKVGRVLD
VIIDEFNDDEGDEPGTRLIGRTKGDAPGIDGQVYLYAGDFAGQVKIGDIVQARIEDSDEYDLYGEVIHTPEWRPNVPLLG
HFGKH
>Mature_484_residues
TRTQEPLPTVAAPKVGFISLGCPKALVDSERILTQLRAEGYEVAPNYEDAQAVIVNTCGFITPAVEESLSAIGEALDATG
KVIVTGCLGERPEKILERHPKVAAITGSEAVDDVMAHVRELLPIELDPFTGLLPVAAPGMRQGDTLAPSVKLTPRHYAYV
KIAEGCNHTCSFCIIPKLRGRQVSRDAGAVLYEAYRLIAGGTKELMIISQDTSAYGVDLRHRTSEFQGEQVRAHLIDLAE
KLGEMGAWVRMHYVYPYPHVERIVELMSQGKILPYLDVPLQHASPAVLKRMRRPGAGKQLDTIRRWREICPELVIRSTFI
VGFPGETEEDFQLLLDFLEEARLDRVGAFTYSDVEEADANALDGAIPEEVKQERLARFMEVAQRISREKLAEKVGRVLDV
IIDEFNDDEGDEPGTRLIGRTKGDAPGIDGQVYLYAGDFAGQVKIGDIVQARIEDSDEYDLYGEVIHTPEWRPNVPLLGH
FGKH

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=501, Percent_Identity=25.3493013972056, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28872784, Length=501, Percent_Identity=25.3493013972056, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=25.1889168765743, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=60.7064017660044, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1786882, Length=392, Percent_Identity=27.0408163265306, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17553146, Length=399, Percent_Identity=25.31328320802, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996771, Length=255, Percent_Identity=25.0980392156863, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356207, Length=498, Percent_Identity=24.4979919678715, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19922432, Length=474, Percent_Identity=24.0506329113924, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_DEIGD (Q1J1F6)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603847.1
- ProteinModelPortal:   Q1J1F6
- SMR:   Q1J1F6
- STRING:   Q1J1F6
- GeneID:   4057458
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0375
- NMPDR:   fig|68909.1.peg.420
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q1J1F6
- ProtClustDB:   PRK14862
- BioCyc:   DGEO319795:DGEO_0375-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53707; Mature: 53576

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTQEPLPTVAAPKVGFISLGCPKALVDSERILTQLRAEGYEVAPNYEDAQAVIVNTCG
CCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEHHCC
FITPAVEESLSAIGEALDATGKVIVTGCLGERPEKILERHPKVAAITGSEAVDDVMAHVR
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHH
ELLPIELDPFTGLLPVAAPGMRQGDTLAPSVKLTPRHYAYVKIAEGCNHTCSFCIIPKLR
HHCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCC
GRQVSRDAGAVLYEAYRLIAGGTKELMIISQDTSAYGVDLRHRTSEFQGEQVRAHLIDLA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHH
EKLGEMGAWVRMHYVYPYPHVERIVELMSQGKILPYLDVPLQHASPAVLKRMRRPGAGKQ
HHHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCH
LDTIRRWREICPELVIRSTFIVGFPGETEEDFQLLLDFLEEARLDRVGAFTYSDVEEADA
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC
NALDGAIPEEVKQERLARFMEVAQRISREKLAEKVGRVLDVIIDEFNDDEGDEPGTRLIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
RTKGDAPGIDGQVYLYAGDFAGQVKIGDIVQARIEDSDEYDLYGEVIHTPEWRPNVPLLG
CCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCCC
HFGKH
CCCCC
>Mature Secondary Structure 
TRTQEPLPTVAAPKVGFISLGCPKALVDSERILTQLRAEGYEVAPNYEDAQAVIVNTCG
CCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEHHCC
FITPAVEESLSAIGEALDATGKVIVTGCLGERPEKILERHPKVAAITGSEAVDDVMAHVR
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHH
ELLPIELDPFTGLLPVAAPGMRQGDTLAPSVKLTPRHYAYVKIAEGCNHTCSFCIIPKLR
HHCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCC
GRQVSRDAGAVLYEAYRLIAGGTKELMIISQDTSAYGVDLRHRTSEFQGEQVRAHLIDLA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHH
EKLGEMGAWVRMHYVYPYPHVERIVELMSQGKILPYLDVPLQHASPAVLKRMRRPGAGKQ
HHHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCH
LDTIRRWREICPELVIRSTFIVGFPGETEEDFQLLLDFLEEARLDRVGAFTYSDVEEADA
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC
NALDGAIPEEVKQERLARFMEVAQRISREKLAEKVGRVLDVIIDEFNDDEGDEPGTRLIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
RTKGDAPGIDGQVYLYAGDFAGQVKIGDIVQARIEDSDEYDLYGEVIHTPEWRPNVPLLG
CCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCCC
HFGKH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA