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The map label for this gene is citS [H]

Identifier: 94984463

GI number: 94984463

Start: 359070

End: 360704

Strand: Reverse

Name: citS [H]

Synonym: Dgeo_0355

Alternate gene names: 94984463

Gene position: 360704-359070 (Counterclockwise)

Preceding gene: 94984465

Following gene: 94984462

Centisome position: 14.62

GC content: 69.91

Gene sequence:

>1635_bases
TTGTTCCGCTCCAGCTCCAACTCTGGCCTCGTCAGCGTGTCCAACCTGGCGCGCGAGGTGGCGCTGCCGCATACGCCCCG
GCTGCTGCGGGTGCAGTCACGGCTGTTTCTCTCAATTGCTGCGGCCTTCCTGCTGCTGGCACTGCCACTGGCCCTGCTGG
CCACCGTCACGCTGCGTGGCACGATTCACCGCACCTATGCCGAACGGGCGCTGCGCGAGTCGCGCCTGATCGCCACCCTG
CCCCCGGTGCGCGCCGCCCTCGAAGGCGACCGCCAAGCGCGCGCCGGTCTGAATGGGCTGATCAATGGGTATCGCGCGCT
GCTGGGCGCCGACTACGTGGTGGTCACAGACCGCAGCACCCGCCGCCTGACCCATCCCAACCCCGCGCGGCTGGGCGAGC
GGATGGTGGGCGGTGACTTCCAGAGCTTCCTGGTGGGCCGCAGCGTCACCGAAACGGTCGAGGGGACACTGGGCCGCTCG
GTGCGGGCCAAGGTACCGGTTCTGGCTGGGGATGGGCGGGTGCTCGGCCTGGCCAGCGTGGGGTTTTTGCTCCCTCGCCT
GGGGGAAGTTTTTCAGGCAGTGGTGCGCGTGGCCTTGCCCTGGTACGCCGGGGCGCTGGTGCTGGCGCTGCTGCTGGCGT
CCCTCTTGGCGCGGCGAGTGCGGCGCGAGATGCTGGACCTCGAACCCGAGCAGATCGCGGGCGGCCTGCTGCACTACCGC
ACGGTCCTCAATGCCCTTGAAGAGGGCGTGCTCGTCGTGCAGGGCGGACAGGTGCATGTGATGAACCCGCAGGCTCGCGC
CCTGCTCGGCGTGGGGAAGGACACAGCGGAGACGCTCCCGGTCCCTCTCTCCCGCCTGCTTCCCACCACGCTGCTCGAAG
ATCCCAGCCCCGAGTCGCCCATTCCGGTGACGGTGCGGGACCGGCCGCTGCTGGTCAGTGTGCGCCCCGCCCCCGATGGC
GCGCAGGTGGTCACGCTGCGCGACTTGGCCCGGATGCGGGCCCTGGCCGATGAGCTGACCCAGGCACGGCGCTACGCCGA
CCTGCTGCGCGCGCAAACCCACGAGTTCACCAACCGGCTGCACACCCTGGCGGGCCTGCTGCAGCTCGGGGAGACGCGGG
AAGCGCTGAACCTGATCTACGCCCAATCGAATCGCCACGCAGCCCATGCCGAGGCGGTGCGGGCTTTGCGCCATGTCCGC
CTCGTCGCACTGGTGCTGGGCAAGTACGAGCGAGCCGCGGAGCTGGGCCTGACGCTCACGCTCGATCCCCTGAGTACCTT
GCCGGATGCCCTGCCGCCTGGGGTGCTTGACCTGATCGAGCTGAGCGTGGGCAACCTGCTTGAGAATGCCTTTGAGGCGC
TGCACGGGCAGCCGAGCGGACAGGTTCACCTGTTGATCGCCTACGATCCTGAAGGGTTGGTTGTGGAGGTGCGCGACAAC
GGCCCCGGCGTCCCTGCCCCGCTGGCGGCCACCCTCACCCAACGGGGCGTCAGCAGCAAAGGGCCGGGGCGCGGCGTGGG
CCTTGCGCTGGTGCAGCGCCGTGCGGACGACCTCGGCGCGACGCTCACCCATGACCGGAGAGCCGACGCGACAGGCCAGC
ACTGGACCCGCTTTACCCTGGAGGTGCCGCTGTGA

Upstream 100 bases:

>100_bases
CCTGGCCGAACAAAATACTTCACCTTATAGCCGCTTATTGTCCGTAGCGTGAACGAAATTCCAGGGAAAGCGCTGAGGGT
CTGCCAGACTGGGGGCAGTG

Downstream 100 bases:

>100_bases
CGTTCATCCGTACCCTGATTGTCGAGGACGACCCGCGCATCGCCAGGCTGCACCGCGGTCAGCTGGAAGGCGCCGGGGAC
TTCGAGGTGCTGGGGACAGC

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MFRSSSNSGLVSVSNLAREVALPHTPRLLRVQSRLFLSIAAAFLLLALPLALLATVTLRGTIHRTYAERALRESRLIATL
PPVRAALEGDRQARAGLNGLINGYRALLGADYVVVTDRSTRRLTHPNPARLGERMVGGDFQSFLVGRSVTETVEGTLGRS
VRAKVPVLAGDGRVLGLASVGFLLPRLGEVFQAVVRVALPWYAGALVLALLLASLLARRVRREMLDLEPEQIAGGLLHYR
TVLNALEEGVLVVQGGQVHVMNPQARALLGVGKDTAETLPVPLSRLLPTTLLEDPSPESPIPVTVRDRPLLVSVRPAPDG
AQVVTLRDLARMRALADELTQARRYADLLRAQTHEFTNRLHTLAGLLQLGETREALNLIYAQSNRHAAHAEAVRALRHVR
LVALVLGKYERAAELGLTLTLDPLSTLPDALPPGVLDLIELSVGNLLENAFEALHGQPSGQVHLLIAYDPEGLVVEVRDN
GPGVPAPLAATLTQRGVSSKGPGRGVGLALVQRRADDLGATLTHDRRADATGQHWTRFTLEVPL

Sequences:

>Translated_544_residues
MFRSSSNSGLVSVSNLAREVALPHTPRLLRVQSRLFLSIAAAFLLLALPLALLATVTLRGTIHRTYAERALRESRLIATL
PPVRAALEGDRQARAGLNGLINGYRALLGADYVVVTDRSTRRLTHPNPARLGERMVGGDFQSFLVGRSVTETVEGTLGRS
VRAKVPVLAGDGRVLGLASVGFLLPRLGEVFQAVVRVALPWYAGALVLALLLASLLARRVRREMLDLEPEQIAGGLLHYR
TVLNALEEGVLVVQGGQVHVMNPQARALLGVGKDTAETLPVPLSRLLPTTLLEDPSPESPIPVTVRDRPLLVSVRPAPDG
AQVVTLRDLARMRALADELTQARRYADLLRAQTHEFTNRLHTLAGLLQLGETREALNLIYAQSNRHAAHAEAVRALRHVR
LVALVLGKYERAAELGLTLTLDPLSTLPDALPPGVLDLIELSVGNLLENAFEALHGQPSGQVHLLIAYDPEGLVVEVRDN
GPGVPAPLAATLTQRGVSSKGPGRGVGLALVQRRADDLGATLTHDRRADATGQHWTRFTLEVPL
>Mature_544_residues
MFRSSSNSGLVSVSNLAREVALPHTPRLLRVQSRLFLSIAAAFLLLALPLALLATVTLRGTIHRTYAERALRESRLIATL
PPVRAALEGDRQARAGLNGLINGYRALLGADYVVVTDRSTRRLTHPNPARLGERMVGGDFQSFLVGRSVTETVEGTLGRS
VRAKVPVLAGDGRVLGLASVGFLLPRLGEVFQAVVRVALPWYAGALVLALLLASLLARRVRREMLDLEPEQIAGGLLHYR
TVLNALEEGVLVVQGGQVHVMNPQARALLGVGKDTAETLPVPLSRLLPTTLLEDPSPESPIPVTVRDRPLLVSVRPAPDG
AQVVTLRDLARMRALADELTQARRYADLLRAQTHEFTNRLHTLAGLLQLGETREALNLIYAQSNRHAAHAEAVRALRHVR
LVALVLGKYERAAELGLTLTLDPLSTLPDALPPGVLDLIELSVGNLLENAFEALHGQPSGQVHLLIAYDPEGLVVEVRDN
GPGVPAPLAATLTQRGVSSKGPGRGVGLALVQRRADDLGATLTHDRRADATGQHWTRFTLEVPL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=529, Percent_Identity=26.6540642722117, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786837, Length=531, Percent_Identity=31.0734463276836, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58644; Mature: 58644

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRSSSNSGLVSVSNLAREVALPHTPRLLRVQSRLFLSIAAAFLLLALPLALLATVTLRG
CCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIHRTYAERALRESRLIATLPPVRAALEGDRQARAGLNGLINGYRALLGADYVVVTDRST
HHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
RRLTHPNPARLGERMVGGDFQSFLVGRSVTETVEGTLGRSVRAKVPVLAGDGRVLGLASV
CCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCEEHHHHH
GFLLPRLGEVFQAVVRVALPWYAGALVLALLLASLLARRVRREMLDLEPEQIAGGLLHYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TVLNALEEGVLVVQGGQVHVMNPQARALLGVGKDTAETLPVPLSRLLPTTLLEDPSPESP
HHHHHHHCCEEEEECCEEEEECCHHHHHHCCCCCCHHCCCCCHHHHCCHHHCCCCCCCCC
IPVTVRDRPLLVSVRPAPDGAQVVTLRDLARMRALADELTQARRYADLLRAQTHEFTNRL
CEEEECCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTLAGLLQLGETREALNLIYAQSNRHAAHAEAVRALRHVRLVALVLGKYERAAELGLTLT
HHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LDPLSTLPDALPPGVLDLIELSVGNLLENAFEALHGQPSGQVHLLIAYDPEGLVVEVRDN
ECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCC
GPGVPAPLAATLTQRGVSSKGPGRGVGLALVQRRADDLGATLTHDRRADATGQHWTRFTL
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
EVPL
EECC
>Mature Secondary Structure
MFRSSSNSGLVSVSNLAREVALPHTPRLLRVQSRLFLSIAAAFLLLALPLALLATVTLRG
CCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIHRTYAERALRESRLIATLPPVRAALEGDRQARAGLNGLINGYRALLGADYVVVTDRST
HHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
RRLTHPNPARLGERMVGGDFQSFLVGRSVTETVEGTLGRSVRAKVPVLAGDGRVLGLASV
CCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCEEHHHHH
GFLLPRLGEVFQAVVRVALPWYAGALVLALLLASLLARRVRREMLDLEPEQIAGGLLHYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TVLNALEEGVLVVQGGQVHVMNPQARALLGVGKDTAETLPVPLSRLLPTTLLEDPSPESP
HHHHHHHCCEEEEECCEEEEECCHHHHHHCCCCCCHHCCCCCHHHHCCHHHCCCCCCCCC
IPVTVRDRPLLVSVRPAPDGAQVVTLRDLARMRALADELTQARRYADLLRAQTHEFTNRL
CEEEECCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTLAGLLQLGETREALNLIYAQSNRHAAHAEAVRALRHVRLVALVLGKYERAAELGLTLT
HHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LDPLSTLPDALPPGVLDLIELSVGNLLENAFEALHGQPSGQVHLLIAYDPEGLVVEVRDN
ECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCC
GPGVPAPLAATLTQRGVSSKGPGRGVGLALVQRRADDLGATLTHDRRADATGQHWTRFTL
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
EVPL
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]