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The map label for this gene is trmL [H]

Identifier: 94984180

GI number: 94984180

Start: 69773

End: 70234

Strand: Reverse

Name: trmL [H]

Synonym: Dgeo_0072

Alternate gene names: 94984180

Gene position: 70234-69773 (Counterclockwise)

Preceding gene: 94984181

Following gene: 94984179

Centisome position: 2.85

GC content: 69.05

Gene sequence:

>462_bases
GTGAGCGGGCCTCTGCTGCATGTGGTGTTGTTCGAGCCGGAGAAGGCGGGCAACGTCGGCAACGTGGCGCGGACCTGTGC
GGTGCTAGGAGCCGACCTACACCTCATTCGGCCTTTCGGCTTTCACCTACACGACCGCGAGTTTCGCCGAGCGGTGATGG
ACTACCTGCAGGGCGTGACGCTCCACGAGCACGCCAACTGGACCGCCTATCAGGCAGCTCTCCCGCCGGAGGCGCGGGTC
TGGGCTTTTTCCACCCACGCCGAGACGTTCCACACGCGCGCAGGCTTTCGCCGAGGCGACCACCTCGTGTTCGGCCCGGA
ATCGCGCGGCCTCCCGGTCTGGCTCCGCGAGGCCCTGCCCGCCCTCAAACTCCCGCAGCCCGGCGGAGGCCGCAGTCTGA
ATCTGGCGGTGGCCGCCGGCGTAGCGGCCTTCGAGGCGGCGCGGCAGATCGAGGGATGGTAG

Upstream 100 bases:

>100_bases
TGAATGAAACTGAGGTGGGTGTGAGCTTCAAGACCTCCGAGGGCCTGGCGCCCGAGCATGTGCAGGTACGGGTCACGGTG
CTGCTCCGGCGGGTGGAGCA

Downstream 100 bases:

>100_bases
GGGCTGAGGGGGCAGGGCACTGCGTCGGGCGCGCAGAGCCGTTCGCATTCGCCACGCCGGAAGCAATCACTCAGCAGCCA
CCAGGTGCACGGTGCCCACA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSGPLLHVVLFEPEKAGNVGNVARTCAVLGADLHLIRPFGFHLHDREFRRAVMDYLQGVTLHEHANWTAYQAALPPEARV
WAFSTHAETFHTRAGFRRGDHLVFGPESRGLPVWLREALPALKLPQPGGGRSLNLAVAAGVAAFEAARQIEGW

Sequences:

>Translated_153_residues
MSGPLLHVVLFEPEKAGNVGNVARTCAVLGADLHLIRPFGFHLHDREFRRAVMDYLQGVTLHEHANWTAYQAALPPEARV
WAFSTHAETFHTRAGFRRGDHLVFGPESRGLPVWLREALPALKLPQPGGGRSLNLAVAAGVAAFEAARQIEGW
>Mature_152_residues
SGPLLHVVLFEPEKAGNVGNVARTCAVLGADLHLIRPFGFHLHDREFRRAVMDYLQGVTLHEHANWTAYQAALPPEARVW
AFSTHAETFHTRAGFRRGDHLVFGPESRGLPVWLREALPALKLPQPGGGRSLNLAVAAGVAAFEAARQIEGW

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=39.5973154362416, Blast_Score=116, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16819; Mature: 16688

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPLLHVVLFEPEKAGNVGNVARTCAVLGADLHLIRPFGFHLHDREFRRAVMDYLQGVT
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCE
LHEHANWTAYQAALPPEARVWAFSTHAETFHTRAGFRRGDHLVFGPESRGLPVWLREALP
EECCCCCEEEEECCCCCCEEEEEECCCHHHHHHCCCCCCCEEEECCCCCCCCEEHHHCCC
ALKLPQPGGGRSLNLAVAAGVAAFEAARQIEGW
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGPLLHVVLFEPEKAGNVGNVARTCAVLGADLHLIRPFGFHLHDREFRRAVMDYLQGVT
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCE
LHEHANWTAYQAALPPEARVWAFSTHAETFHTRAGFRRGDHLVFGPESRGLPVWLREALP
EECCCCCEEEEECCCCCCEEEEEECCCHHHHHHCCCCCCCEEEECCCCCCCCEEHHHCCC
ALKLPQPGGGRSLNLAVAAGVAAFEAARQIEGW
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA