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The map label for this gene is ispF [H]

Identifier: 94984181

GI number: 94984181

Start: 70231

End: 70722

Strand: Reverse

Name: ispF [H]

Synonym: Dgeo_0073

Alternate gene names: 94984181

Gene position: 70722-70231 (Counterclockwise)

Preceding gene: 94984182

Following gene: 94984180

Centisome position: 2.87

GC content: 68.5

Gene sequence:

>492_bases
ATGACCCCTTCCCCCCTTCCCTACCGCATCGGCTACGGAGAAGACGCGCACCGCCTGGCCGAAGGGCGAACGCTGGTGCT
GGGCGGCGTCCCGATCCCCCACGCCGAACGCGGCGCGGTGGCACACAGTGACGGGGACGCTGTATTGCACGCGCTGGCCG
ACGCACTGCTCTCGGGCATGAGCTTGGGCGACATCGGGCAGTATTACCCCGACACCGACCCTGCCCACGCGGGGCTGGAC
TCGCGGGTGATTCTGGCCGACAGCCTGGCGCTGGTGCGCGAGTGGAAGTACGTTCCGGCAAACGTCGCGCTGGTGATCAC
CCTCGACCGCCCCAAATTGGGGCCGCTGCGCGCCGACATCGCCCGCAACGTGGCTGCCCTGCTGGGCCTGAATGAAACTG
AGGTGGGTGTGAGCTTCAAGACCTCCGAGGGCCTGGCGCCCGAGCATGTGCAGGTACGGGTCACGGTGCTGCTCCGGCGG
GTGGAGCAGTGA

Upstream 100 bases:

>100_bases
GAGCCAACAAGGCTGAAGCCCCGCTCTCTGTCCCCTGCCCTCTCCTACGACTCGTAGAGCTGCGGAGCAGAGCTTTGCAA
GTCTGCGTTACAGTCCCCCC

Downstream 100 bases:

>100_bases
GCGGGCCTCTGCTGCATGTGGTGTTGTTCGAGCCGGAGAAGGCGGGCAACGTCGGCAACGTGGCGCGGACCTGTGCGGTG
CTAGGAGCCGACCTACACCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTPSPLPYRIGYGEDAHRLAEGRTLVLGGVPIPHAERGAVAHSDGDAVLHALADALLSGMSLGDIGQYYPDTDPAHAGLD
SRVILADSLALVREWKYVPANVALVITLDRPKLGPLRADIARNVAALLGLNETEVGVSFKTSEGLAPEHVQVRVTVLLRR
VEQ

Sequences:

>Translated_163_residues
MTPSPLPYRIGYGEDAHRLAEGRTLVLGGVPIPHAERGAVAHSDGDAVLHALADALLSGMSLGDIGQYYPDTDPAHAGLD
SRVILADSLALVREWKYVPANVALVITLDRPKLGPLRADIARNVAALLGLNETEVGVSFKTSEGLAPEHVQVRVTVLLRR
VEQ
>Mature_162_residues
TPSPLPYRIGYGEDAHRLAEGRTLVLGGVPIPHAERGAVAHSDGDAVLHALADALLSGMSLGDIGQYYPDTDPAHAGLDS
RVILADSLALVREWKYVPANVALVITLDRPKLGPLRADIARNVAALLGLNETEVGVSFKTSEGLAPEHVQVRVTVLLRRV
EQ

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=137, Percent_Identity=42.3357664233577, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17383; Mature: 17252

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSPLPYRIGYGEDAHRLAEGRTLVLGGVPIPHAERGAVAHSDGDAVLHALADALLSGM
CCCCCCCEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCC
SLGDIGQYYPDTDPAHAGLDSRVILADSLALVREWKYVPANVALVITLDRPKLGPLRADI
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
ARNVAALLGLNETEVGVSFKTSEGLAPEHVQVRVTVLLRRVEQ
HHHHHHHHCCCCHHCCEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPSPLPYRIGYGEDAHRLAEGRTLVLGGVPIPHAERGAVAHSDGDAVLHALADALLSGM
CCCCCCEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCC
SLGDIGQYYPDTDPAHAGLDSRVILADSLALVREWKYVPANVALVITLDRPKLGPLRADI
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
ARNVAALLGLNETEVGVSFKTSEGLAPEHVQVRVTVLLRRVEQ
HHHHHHHHCCCCHHCCEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]