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The map label for this gene is purC

Identifier: 94984175

GI number: 94984175

Start: 65028

End: 65753

Strand: Reverse

Name: purC

Synonym: Dgeo_0067

Alternate gene names: 94984175

Gene position: 65753-65028 (Counterclockwise)

Preceding gene: 94984176

Following gene: 94984174

Centisome position: 2.67

GC content: 64.05

Gene sequence:

>726_bases
ATGAAGCTTGAGCAACGGTACGAAGGCAAGGCGAAAAAGGTCTACGCGACCGAGAACCCCTTGGAATACGTGGTGGAGTA
CAAGGACGACGCCACCGCTTTCAACGGCGTGAAACGCGCCCAGATTGTGGGCAAGGGCCAGATCAACAACGCGATCACCG
CCCACCTCTTTCCCTTGCTGGAGGAGGCGGGCGTGCCCACCCATTTTCTCGAGAAGCTCAGCGAGCGCGAGCAGCGTGTC
CGGGCCGTCACGATCATCCCGGTCGAGGTGATTGTGCGGAATGTCGCGGCGGGCAGCTTCGCCAAGAGGCTCGGGCTGGA
GGAAGGGACGCCTCTTGCCCGCCCGGTCGTCGAGTACTGCCTCAAGAGTGACGCGCTCGGTGATCCCCTGATCAACACCG
ACACGGCAGTGGCCCTGGGCTGGGCCAGCGAAGACGACCTGAAGCGCATCCGCGAACTCGCGCTGAAGGTGCGTGATTTC
CTCACACCGTACTTTGCGGCGCGGGGCATCCGGCTGATTGACTTCAAGCTGGAGTTTGGGCGCACCCACGACGGCCAGAT
CGTCCTCGCCGACGAGATCAGCCCGGACACCTGCCGCTTCTGGGATGCCGCGACCAACGAGAAGCTGGACAAGGACCGTT
TCCGCCGCGACCTTGGCGGCGTGGAGGACGCGTATGCGGAGATGCTGCGGCGCGTGACGGGGGAAGGGGGCCGGGATGAG
GGTTGA

Upstream 100 bases:

>100_bases
GCTCGCCCATGAACAACCACGCACAATCCAGGAGCGTCTACCCGCCAGCACTGCCGGGAGACGTTCGCCCTACATTTCCT
TTCCGTGCGGGAGGCCTGGC

Downstream 100 bases:

>100_bases
GGCACAAACCGGTACGTTCCACTTTTCCTCACCCTTCACACCTAACCCCTGAGGTTCCCATGCCCACCTACCACGCCAAA
GTCTTTGTCACCCTCAAGCC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKLEQRYEGKAKKVYATENPLEYVVEYKDDATAFNGVKRAQIVGKGQINNAITAHLFPLLEEAGVPTHFLEKLSEREQRV
RAVTIIPVEVIVRNVAAGSFAKRLGLEEGTPLARPVVEYCLKSDALGDPLINTDTAVALGWASEDDLKRIRELALKVRDF
LTPYFAARGIRLIDFKLEFGRTHDGQIVLADEISPDTCRFWDAATNEKLDKDRFRRDLGGVEDAYAEMLRRVTGEGGRDE
G

Sequences:

>Translated_241_residues
MKLEQRYEGKAKKVYATENPLEYVVEYKDDATAFNGVKRAQIVGKGQINNAITAHLFPLLEEAGVPTHFLEKLSEREQRV
RAVTIIPVEVIVRNVAAGSFAKRLGLEEGTPLARPVVEYCLKSDALGDPLINTDTAVALGWASEDDLKRIRELALKVRDF
LTPYFAARGIRLIDFKLEFGRTHDGQIVLADEISPDTCRFWDAATNEKLDKDRFRRDLGGVEDAYAEMLRRVTGEGGRDE
G
>Mature_241_residues
MKLEQRYEGKAKKVYATENPLEYVVEYKDDATAFNGVKRAQIVGKGQINNAITAHLFPLLEEAGVPTHFLEKLSEREQRV
RAVTIIPVEVIVRNVAAGSFAKRLGLEEGTPLARPVVEYCLKSDALGDPLINTDTAVALGWASEDDLKRIRELALKVRDF
LTPYFAARGIRLIDFKLEFGRTHDGQIVLADEISPDTCRFWDAATNEKLDKDRFRRDLGGVEDAYAEMLRRVTGEGGRDE
G

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=234, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI5453539, Length=234, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI119220559, Length=241, Percent_Identity=29.4605809128631, Blast_Score=98, Evalue=6e-21,
Organism=Escherichia coli, GI1788820, Length=226, Percent_Identity=46.0176991150442, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17531275, Length=240, Percent_Identity=33.75, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18860083, Length=232, Percent_Identity=32.7586206896552, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24583917, Length=232, Percent_Identity=30.6034482758621, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_DEIGD (Q1J2B4)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603539.1
- ProteinModelPortal:   Q1J2B4
- SMR:   Q1J2B4
- STRING:   Q1J2B4
- GeneID:   4058508
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0067
- NMPDR:   fig|68909.1.peg.1520
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- PhylomeDB:   Q1J2B4
- ProtClustDB:   PRK09362
- BioCyc:   DGEO319795:DGEO_0067-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27010; Mature: 27010

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEQRYEGKAKKVYATENPLEYVVEYKDDATAFNGVKRAQIVGKGQINNAITAHLFPLL
CCCCHHHCCCCEEEEECCCCHHHHEECCCCHHHHCCCHHHHEECCCCCCHHHHHHHHHHH
EEAGVPTHFLEKLSEREQRVRAVTIIPVEVIVRNVAAGSFAKRLGLEEGTPLARPVVEYC
HHCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LKSDALGDPLINTDTAVALGWASEDDLKRIRELALKVRDFLTPYFAARGIRLIDFKLEFG
HHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
RTHDGQIVLADEISPDTCRFWDAATNEKLDKDRFRRDLGGVEDAYAEMLRRVTGEGGRDE
CCCCCCEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
G
C
>Mature Secondary Structure
MKLEQRYEGKAKKVYATENPLEYVVEYKDDATAFNGVKRAQIVGKGQINNAITAHLFPLL
CCCCHHHCCCCEEEEECCCCHHHHEECCCCHHHHCCCHHHHEECCCCCCHHHHHHHHHHH
EEAGVPTHFLEKLSEREQRVRAVTIIPVEVIVRNVAAGSFAKRLGLEEGTPLARPVVEYC
HHCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LKSDALGDPLINTDTAVALGWASEDDLKRIRELALKVRDFLTPYFAARGIRLIDFKLEFG
HHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
RTHDGQIVLADEISPDTCRFWDAATNEKLDKDRFRRDLGGVEDAYAEMLRRVTGEGGRDE
CCCCCCEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA