Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 94984176

GI number: 94984176

Start: 66048

End: 66899

Strand: Reverse

Name: hemK [H]

Synonym: Dgeo_0068

Alternate gene names: 94984176

Gene position: 66899-66048 (Counterclockwise)

Preceding gene: 94984177

Following gene: 94984175

Centisome position: 2.71

GC content: 72.07

Gene sequence:

>852_bases
GTGACTGCCACCCTCACCCTCCACACCTGGTTGCAGGAGGCTACGCGCCTGCTGCGTGACGCGGGTGTCCCCTCGCCTGA
GGCAGACGCGCGGGCCCTGGTGCAGCACGCCCTGAACCTGGGCGGGGTAGCACTCCTCACGCGCGGCACCGAGCCGGTTG
CAGAGGCGGACGCGGCCCGGCTCGTCAACCTGCTGCGGCGCCGGGCGGCCCGCGAGCCGCTCCAGCACCTGCTGGGCGAG
GTGGAGTGGGGCGGCGTGCGCCTGCGGACGGACCGGCGGGCGCTGGTGCCGCGGCCCGAGACAGAATGGCTGCTGCACCT
CGCGCTGGAAACGCTCCAGGGTGTGTCCGCACCCCGCGTGCTGGACGTGGGCACCGGGACGGGGGCACTCGCGCTGGGCA
TCAAGGCCGCCCGCCCAGACGCCAGCGTAACCGCCACCGATCTCAGCCCCGACGCACTGACGCTGGCGCGGGAAAACGCT
GTGCTCAACGGTCTGGATGTCGTCTTTCTGGCAGGTCGCCTGCTGGCGGGCCTGTCCGGACCCTTCGACCTGATCGTGAG
TAATCCCCCCTACCTGCCCGCAGCCGACCGTGAGCAGGTCGATCCGGAAGTGCGTTTTGACCCCGACCTGGCGCTGTATG
CCGGGCCAGAGGGTCTGGACGTGGCGCGGCCCCTGGCGGTGGAGGCGCAGGCCGCGCTCGCCCCGGGCAGCGTGCTGCTG
CTGGAACTCGACCCGCGCAACGCTGCTCCCTTTGCCGCCGAACTCCGCGAGCAGGGCTGGCAGGTGGCCGTTCTCCCCGA
TCTGGCGGGGCGAGAACGATTTGTGCGGGCCAACCGGCGTGCAGAGGGGTGA

Upstream 100 bases:

>100_bases
GAGTCGGTCGGGGAGGGGGCGGCGCGGCGGCTGATCATCAAGCCAAGGCCCCATCAGCCATGAGCGAGCGGGCCGGCCCC
ACCGCCAGAGCGTTGCGCCT

Downstream 100 bases:

>100_bases
ATTTTACCCGGAGTTCTCGCCCACCCCCCTGAACGAGTTCACCTCGCTGCCCTTCATGAGGTGTTAAGGTGACCTCCGGC
AATCCTTCGCCCTCTGCGGC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTATLTLHTWLQEATRLLRDAGVPSPEADARALVQHALNLGGVALLTRGTEPVAEADAARLVNLLRRRAAREPLQHLLGE
VEWGGVRLRTDRRALVPRPETEWLLHLALETLQGVSAPRVLDVGTGTGALALGIKAARPDASVTATDLSPDALTLARENA
VLNGLDVVFLAGRLLAGLSGPFDLIVSNPPYLPAADREQVDPEVRFDPDLALYAGPEGLDVARPLAVEAQAALAPGSVLL
LELDPRNAAPFAAELREQGWQVAVLPDLAGRERFVRANRRAEG

Sequences:

>Translated_283_residues
MTATLTLHTWLQEATRLLRDAGVPSPEADARALVQHALNLGGVALLTRGTEPVAEADAARLVNLLRRRAAREPLQHLLGE
VEWGGVRLRTDRRALVPRPETEWLLHLALETLQGVSAPRVLDVGTGTGALALGIKAARPDASVTATDLSPDALTLARENA
VLNGLDVVFLAGRLLAGLSGPFDLIVSNPPYLPAADREQVDPEVRFDPDLALYAGPEGLDVARPLAVEAQAALAPGSVLL
LELDPRNAAPFAAELREQGWQVAVLPDLAGRERFVRANRRAEG
>Mature_282_residues
TATLTLHTWLQEATRLLRDAGVPSPEADARALVQHALNLGGVALLTRGTEPVAEADAARLVNLLRRRAAREPLQHLLGEV
EWGGVRLRTDRRALVPRPETEWLLHLALETLQGVSAPRVLDVGTGTGALALGIKAARPDASVTATDLSPDALTLARENAV
LNGLDVVFLAGRLLAGLSGPFDLIVSNPPYLPAADREQVDPEVRFDPDLALYAGPEGLDVARPLAVEAQAALAPGSVLLL
ELDPRNAAPFAAELREQGWQVAVLPDLAGRERFVRANRRAEG

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=287, Percent_Identity=31.7073170731707, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1787463, Length=240, Percent_Identity=36.25, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082085, Length=198, Percent_Identity=33.8383838383838, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790830, Length=143, Percent_Identity=28.6713286713287, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582226, Length=298, Percent_Identity=30.2013422818792, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30210; Mature: 30079

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLTLHTWLQEATRLLRDAGVPSPEADARALVQHALNLGGVALLTRGTEPVAEADAAR
CCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LVNLLRRRAAREPLQHLLGEVEWGGVRLRTDRRALVPRPETEWLLHLALETLQGVSAPRV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
LDVGTGTGALALGIKAARPDASVTATDLSPDALTLARENAVLNGLDVVFLAGRLLAGLSG
EEECCCCCEEEECEEECCCCCCEEEECCCCCHHHHHHHCHHHCCHHHHHHHHHHHHCCCC
PFDLIVSNPPYLPAADREQVDPEVRFDPDLALYAGPEGLDVARPLAVEAQAALAPGSVLL
CEEEEECCCCCCCCCCHHHCCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEE
LELDPRNAAPFAAELREQGWQVAVLPDLAGRERFVRANRRAEG
EEECCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TATLTLHTWLQEATRLLRDAGVPSPEADARALVQHALNLGGVALLTRGTEPVAEADAAR
CEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LVNLLRRRAAREPLQHLLGEVEWGGVRLRTDRRALVPRPETEWLLHLALETLQGVSAPRV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
LDVGTGTGALALGIKAARPDASVTATDLSPDALTLARENAVLNGLDVVFLAGRLLAGLSG
EEECCCCCEEEECEEECCCCCCEEEECCCCCHHHHHHHCHHHCCHHHHHHHHHHHHCCCC
PFDLIVSNPPYLPAADREQVDPEVRFDPDLALYAGPEGLDVARPLAVEAQAALAPGSVLL
CEEEEECCCCCCCCCCHHHCCCCCEECCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEE
LELDPRNAAPFAAELREQGWQVAVLPDLAGRERFVRANRRAEG
EEECCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]