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The map label for this gene is topB [H]

Identifier: 94995092

GI number: 94995092

Start: 1637352

End: 1639085

Strand: Direct

Name: topB [H]

Synonym: MGAS10750_Spy1696

Alternate gene names: 94995092

Gene position: 1637352-1639085 (Clockwise)

Preceding gene: 94995091

Following gene: 94995093

Centisome position: 84.53

GC content: 31.37

Gene sequence:

>1734_bases
ATGGAGGTATTAATGAAGTTAGTAATAGCAGAAAAGCCAAGTGTAGCAGTTACAATTGCAAAAATAATTGGAGCAAGAAC
AAGAAAAAACGGATATTATGAGGGGAATGGATACATTGTTTCTTGGTGTGTAGGTCATTTAATTCAAATGGCAAGTCCCG
ATAAGATAGACGAAAAATGGAAGAAATGGACAATAGAAAACCTTCCTATAATTCCAGAAGAATATATTTATGAAGTATCT
AAAAGTACTAAAAAACAATATGGAGTTTTAAAGAAACTTTTAAACGATAAGAACATCAACATAGTTATAAATGCTTGTGA
TGCTGGAAGAGAGGGAGAACTTATTTTTAGGCTTGTATATAATCAAGCTAAATGTAAGAAGAAGATTCAAAGACTTTGGA
TATCTTCAATGGAAAACGAAGCTATTGAAGATGGATTTAGAAATCTTAAAGACGGAGAAAAATTTGAAGACTTATATAGA
TCAGCAAGTGCAAGAGCAATTGCGGATTGGCTGGTGGGAATGAATTTAAGTAGGCTTTATTCTTGCATTTACAAGGAAAC
ATATTCAGTTGGTAGAGTACAAACACCAACTCTATATTTAATAGCTAAAAGGGATAGCGAAATAAACCTATTTAAGAAGC
AAAAATATTATACAGTTGACCTATCTTATGGAGGATTGAAACTTGTATCAGATAGGATTGATAGAATTGAAGTTGCAGAA
CAACTATTAAATCTACTAGAAGATGAAATAGTTATTACAGAGGTAGAAGATAAAGAGGTAAGCACAAAACCAGATAAGCC
TTATGATCTCACTACCTTACAAAGAGAAGCAAATAAATATTTTGGATATTCAGCAAATGACACTTTAAATCTAGCACAAG
CTTTGTATGAAAAAAAGCTAATCACATATCCAAGAACAGATAGCAGGTATTTAACCGATGACATGGTTAATACTATGAAA
GAATTATTAGAAGGACTTGAAGAAGATTTTAAACTCAACAAATCAAACTTTAAGTCTATTTTTAATTCATCTAAGGTTAC
AGACCACTATGCAATTATTCCTACTATATCAGGTATTGGAAAAGCCAAAGATTTATCTGATAAAGAAAGCAAAGTCTATA
ATCTAATTAAGGATAAACTACTTGCTTCATGTTCGGATAACTTAAAGGAATCTAGTAGAAAAATCAGATATGAATATGAT
AAATTTAACTTCAATGCAAGTGGAAAGACTATAATCGATGAAGGTTATACCAAGTATCTAAAGGCTTATGGAAAAGAAAA
GCAAGAAAATGAATTACCAGATGTAAAGACTGGAGATAAAATTAAGCTAACTTCTAAAAATATATCGGAGAAATTTACAA
AAGCTCCAAGTCATTATAATGAAGATACACTTTTAAAGGCTATGGAAAATGCAGGAGTAGAATCCTTGGATAAAGACATA
GAAGTGGAGAGAAAAGGCTTAGGAACGCCAGCTACGAGAGCAGGAATTATTGAAAATCTTATCCATAAGGAACTCATAAG
AAGAGATAAGAAAAATTTACTTGTAACAGAAAAAGGCAATAGGCTTGTATCTATTGTAGAGGATAAGTTTAAATCAGCAG
AAACAACATCTGAATGGGAAATGAAACTTGCGAAGATTAACTCTGGCGAAGTAGATAAAGAAGACTTTTTAAGAGAAATA
GAAGATAGTATAAGGGAGCTTGTAGATAGGTACAAGAATAATCTAAATGAATAA

Upstream 100 bases:

>100_bases
TCACGAAGAATCTTTAGATGAAGACGATGAAGATGATGAACTATTATAATAAATTTTAAAAGCTTGAGCGAGAGAGAAAT
CTTTCGCTCTTTTTTTATTT

Downstream 100 bases:

>100_bases
GGTAAAAATATTAGAGCTTTTCGGCGGCATAGGTGCTATTAGAAAGGCTTTTATTAATTTAAAGATACCTTATGAAGTAG
TTGATTATGTAGAAATAGAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MEVLMKLVIAEKPSVAVTIAKIIGARTRKNGYYEGNGYIVSWCVGHLIQMASPDKIDEKWKKWTIENLPIIPEEYIYEVS
KSTKKQYGVLKKLLNDKNINIVINACDAGREGELIFRLVYNQAKCKKKIQRLWISSMENEAIEDGFRNLKDGEKFEDLYR
SASARAIADWLVGMNLSRLYSCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLSYGGLKLVSDRIDRIEVAE
QLLNLLEDEIVITEVEDKEVSTKPDKPYDLTTLQREANKYFGYSANDTLNLAQALYEKKLITYPRTDSRYLTDDMVNTMK
ELLEGLEEDFKLNKSNFKSIFNSSKVTDHYAIIPTISGIGKAKDLSDKESKVYNLIKDKLLASCSDNLKESSRKIRYEYD
KFNFNASGKTIIDEGYTKYLKAYGKEKQENELPDVKTGDKIKLTSKNISEKFTKAPSHYNEDTLLKAMENAGVESLDKDI
EVERKGLGTPATRAGIIENLIHKELIRRDKKNLLVTEKGNRLVSIVEDKFKSAETTSEWEMKLAKINSGEVDKEDFLREI
EDSIRELVDRYKNNLNE

Sequences:

>Translated_577_residues
MEVLMKLVIAEKPSVAVTIAKIIGARTRKNGYYEGNGYIVSWCVGHLIQMASPDKIDEKWKKWTIENLPIIPEEYIYEVS
KSTKKQYGVLKKLLNDKNINIVINACDAGREGELIFRLVYNQAKCKKKIQRLWISSMENEAIEDGFRNLKDGEKFEDLYR
SASARAIADWLVGMNLSRLYSCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLSYGGLKLVSDRIDRIEVAE
QLLNLLEDEIVITEVEDKEVSTKPDKPYDLTTLQREANKYFGYSANDTLNLAQALYEKKLITYPRTDSRYLTDDMVNTMK
ELLEGLEEDFKLNKSNFKSIFNSSKVTDHYAIIPTISGIGKAKDLSDKESKVYNLIKDKLLASCSDNLKESSRKIRYEYD
KFNFNASGKTIIDEGYTKYLKAYGKEKQENELPDVKTGDKIKLTSKNISEKFTKAPSHYNEDTLLKAMENAGVESLDKDI
EVERKGLGTPATRAGIIENLIHKELIRRDKKNLLVTEKGNRLVSIVEDKFKSAETTSEWEMKLAKINSGEVDKEDFLREI
EDSIRELVDRYKNNLNE
>Mature_577_residues
MEVLMKLVIAEKPSVAVTIAKIIGARTRKNGYYEGNGYIVSWCVGHLIQMASPDKIDEKWKKWTIENLPIIPEEYIYEVS
KSTKKQYGVLKKLLNDKNINIVINACDAGREGELIFRLVYNQAKCKKKIQRLWISSMENEAIEDGFRNLKDGEKFEDLYR
SASARAIADWLVGMNLSRLYSCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLSYGGLKLVSDRIDRIEVAE
QLLNLLEDEIVITEVEDKEVSTKPDKPYDLTTLQREANKYFGYSANDTLNLAQALYEKKLITYPRTDSRYLTDDMVNTMK
ELLEGLEEDFKLNKSNFKSIFNSSKVTDHYAIIPTISGIGKAKDLSDKESKVYNLIKDKLLASCSDNLKESSRKIRYEYD
KFNFNASGKTIIDEGYTKYLKAYGKEKQENELPDVKTGDKIKLTSKNISEKFTKAPSHYNEDTLLKAMENAGVESLDKDI
EVERKGLGTPATRAGIIENLIHKELIRRDKKNLLVTEKGNRLVSIVEDKFKSAETTSEWEMKLAKINSGEVDKEDFLREI
EDSIRELVDRYKNNLNE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1788061, Length=616, Percent_Identity=32.3051948051948, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1787529, Length=573, Percent_Identity=27.5741710296684, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32563869, Length=574, Percent_Identity=23.6933797909408, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=658, Percent_Identity=22.7963525835866, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640096, Length=606, Percent_Identity=22.7722772277228, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 66355; Mature: 66355

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLMKLVIAEKPSVAVTIAKIIGARTRKNGYYEGNGYIVSWCVGHLIQMASPDKIDEKW
CHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCEECCCCEEHHHHHHHHHHHCCCCHHHHHH
KKWTIENLPIIPEEYIYEVSKSTKKQYGVLKKLLNDKNINIVINACDAGREGELIFRLVY
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHH
NQAKCKKKIQRLWISSMENEAIEDGFRNLKDGEKFEDLYRSASARAIADWLVGMNLSRLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLSYGGLKLVSDRIDRIEVAE
HHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
QLLNLLEDEIVITEVEDKEVSTKPDKPYDLTTLQREANKYFGYSANDTLNLAQALYEKKL
HHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ITYPRTDSRYLTDDMVNTMKELLEGLEEDFKLNKSNFKSIFNSSKVTDHYAIIPTISGIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCC
KAKDLSDKESKVYNLIKDKLLASCSDNLKESSRKIRYEYDKFNFNASGKTIIDEGYTKYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEECCCCCEEHHHHHHHHH
KAYGKEKQENELPDVKTGDKIKLTSKNISEKFTKAPSHYNEDTLLKAMENAGVESLDKDI
HHHCCCCHHCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
EVERKGLGTPATRAGIIENLIHKELIRRDKKNLLVTEKGNRLVSIVEDKFKSAETTSEWE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCHHHH
MKLAKINSGEVDKEDFLREIEDSIRELVDRYKNNLNE
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEVLMKLVIAEKPSVAVTIAKIIGARTRKNGYYEGNGYIVSWCVGHLIQMASPDKIDEKW
CHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCEECCCCEEHHHHHHHHHHHCCCCHHHHHH
KKWTIENLPIIPEEYIYEVSKSTKKQYGVLKKLLNDKNINIVINACDAGREGELIFRLVY
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHH
NQAKCKKKIQRLWISSMENEAIEDGFRNLKDGEKFEDLYRSASARAIADWLVGMNLSRLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLSYGGLKLVSDRIDRIEVAE
HHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
QLLNLLEDEIVITEVEDKEVSTKPDKPYDLTTLQREANKYFGYSANDTLNLAQALYEKKL
HHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ITYPRTDSRYLTDDMVNTMKELLEGLEEDFKLNKSNFKSIFNSSKVTDHYAIIPTISGIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCC
KAKDLSDKESKVYNLIKDKLLASCSDNLKESSRKIRYEYDKFNFNASGKTIIDEGYTKYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEECCCCCEEHHHHHHHHH
KAYGKEKQENELPDVKTGDKIKLTSKNISEKFTKAPSHYNEDTLLKAMENAGVESLDKDI
HHHCCCCHHCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
EVERKGLGTPATRAGIIENLIHKELIRRDKKNLLVTEKGNRLVSIVEDKFKSAETTSEWE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCHHHH
MKLAKINSGEVDKEDFLREIEDSIRELVDRYKNNLNE
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA