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The map label for this gene is scrFIBM [H]

Identifier: 94995093

GI number: 94995093

Start: 1639078

End: 1640043

Strand: Direct

Name: scrFIBM [H]

Synonym: MGAS10750_Spy1697

Alternate gene names: 94995093

Gene position: 1639078-1640043 (Clockwise)

Preceding gene: 94995092

Following gene: 94995094

Centisome position: 84.61

GC content: 31.16

Gene sequence:

>966_bases
ATGAATAAGGTAAAAATATTAGAGCTTTTCGGCGGCATAGGTGCTATTAGAAAGGCTTTTATTAATTTAAAGATACCTTA
TGAAGTAGTTGATTATGTAGAAATAGATAGGGCTTGTGTTAAATCATACAACGCACTTTATGGAGAAGACTATAAGCCAA
AATCAGTAGTAGAATATAAAGCTCCTAATGCGAAGATAGACTTAGTTATGCATGGAAGTCCTTGCCAAGACTTTTCAAGA
ATTGGAAAAAAGAAAGGAGGAGTAAAAAACTCAGGAACTAGATCAAGCCTACTATTTGAAACAATTAGAATAATCAAAGA
AATGAAAGATAAACCTAAATGGATAATTTGGGAAAATGTAAAGGGAGTCCTTGATAGAAATATGAGGGACTCCTTTTTTA
TTTATCTAAAAGAGCTAGAAAACCTTGGATATGAAAGCAAGTATGAAATCTTAAATGCAATGGACTTTGGGATACCTCAA
AAAAGAGAAAGGATATTTGTTGTTTCATGTCTAGGAGCAAATAACTTTTCTTTTGATAAATTAGAGAGGAAAGAAACAAG
ACCACTAAGTGAATTTTTAGAAAAGAATGTAAGTGAACTTTATACAATGACCCAACCTTATATGCTGAAATTTTTAAATA
AAGGAATAAATAACAGTTTTAGAGGGCGACTCAAAGTGATTAAAGATTTTTCTTATACTATTTCTACAAAACAGATGAGA
GTACCTAATTCTGGAATAATAGATATTGGAAATGGTCAGTATAGGTATTTAACAGAAAGAGAATGTTTAAGACTAATGGG
TTTTGATGACAGTGATATTAACAAACTAGAAGAAGCACATCCAAGAAGAAAAAATTGCACTTCAAGTAAACTATATAAGC
AGGCCGGCAATTCTATTGTGGTCGATATTTTAATGGCTATTATAAAAGAAATTCGTAGAATGGAGGTAGGAAATGCAAGT
AAATGA

Upstream 100 bases:

>100_bases
CTTGCGAAGATTAACTCTGGCGAAGTAGATAAAGAAGACTTTTTAAGAGAAATAGAAGATAGTATAAGGGAGCTTGTAGA
TAGGTACAAGAATAATCTAA

Downstream 100 bases:

>100_bases
TTTTAAGAATATACAAGAAGCAATAAAGTATGAAGTTTTGCAAGATGAAAAAGAATATTTAAAACTCTTAAAAGTTATAG
GAAATAATCAGAAATATGAT

Product: Type II restriction-modification system methylation subunit

Products: NA

Alternate protein names: M.ScrFI-B; M.ScrFIB; Cytosine-specific methyltransferase ScrFIB [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDRACVKSYNALYGEDYKPKSVVEYKAPNAKIDLVMHGSPCQDFSR
IGKKKGGVKNSGTRSSLLFETIRIIKEMKDKPKWIIWENVKGVLDRNMRDSFFIYLKELENLGYESKYEILNAMDFGIPQ
KRERIFVVSCLGANNFSFDKLERKETRPLSEFLEKNVSELYTMTQPYMLKFLNKGINNSFRGRLKVIKDFSYTISTKQMR
VPNSGIIDIGNGQYRYLTERECLRLMGFDDSDINKLEEAHPRRKNCTSSKLYKQAGNSIVVDILMAIIKEIRRMEVGNAS
K

Sequences:

>Translated_321_residues
MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDRACVKSYNALYGEDYKPKSVVEYKAPNAKIDLVMHGSPCQDFSR
IGKKKGGVKNSGTRSSLLFETIRIIKEMKDKPKWIIWENVKGVLDRNMRDSFFIYLKELENLGYESKYEILNAMDFGIPQ
KRERIFVVSCLGANNFSFDKLERKETRPLSEFLEKNVSELYTMTQPYMLKFLNKGINNSFRGRLKVIKDFSYTISTKQMR
VPNSGIIDIGNGQYRYLTERECLRLMGFDDSDINKLEEAHPRRKNCTSSKLYKQAGNSIVVDILMAIIKEIRRMEVGNAS
K
>Mature_321_residues
MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDRACVKSYNALYGEDYKPKSVVEYKAPNAKIDLVMHGSPCQDFSR
IGKKKGGVKNSGTRSSLLFETIRIIKEMKDKPKWIIWENVKGVLDRNMRDSFFIYLKELENLGYESKYEILNAMDFGIPQ
KRERIFVVSCLGANNFSFDKLERKETRPLSEFLEKNVSELYTMTQPYMLKFLNKGINNSFRGRLKVIKDFSYTISTKQMR
VPNSGIIDIGNGQYRYLTERECLRLMGFDDSDINKLEEAHPRRKNCTSSKLYKQAGNSIVVDILMAIIKEIRRMEVGNAS
K

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=184, Percent_Identity=28.2608695652174, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 37106; Mature: 37106

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDRACVKSYNALYGEDYKPKSVVEYK
CCCEEHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
APNAKIDLVMHGSPCQDFSRIGKKKGGVKNSGTRSSLLFETIRIIKEMKDKPKWIIWENV
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCH
KGVLDRNMRDSFFIYLKELENLGYESKYEILNAMDFGIPQKRERIFVVSCLGANNFSFDK
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHH
LERKETRPLSEFLEKNVSELYTMTQPYMLKFLNKGINNSFRGRLKVIKDFSYTISTKQMR
HHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEEE
VPNSGIIDIGNGQYRYLTERECLRLMGFDDSDINKLEEAHPRRKNCTSSKLYKQAGNSIV
CCCCCEEECCCCCEEEECHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHH
VDILMAIIKEIRRMEVGNASK
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDRACVKSYNALYGEDYKPKSVVEYK
CCCEEHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
APNAKIDLVMHGSPCQDFSRIGKKKGGVKNSGTRSSLLFETIRIIKEMKDKPKWIIWENV
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCH
KGVLDRNMRDSFFIYLKELENLGYESKYEILNAMDFGIPQKRERIFVVSCLGANNFSFDK
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHH
LERKETRPLSEFLEKNVSELYTMTQPYMLKFLNKGINNSFRGRLKVIKDFSYTISTKQMR
HHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEEE
VPNSGIIDIGNGQYRYLTERECLRLMGFDDSDINKLEEAHPRRKNCTSSKLYKQAGNSIV
CCCCCEEECCCCCEEEECHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHH
VDILMAIIKEIRRMEVGNASK
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA