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The map label for this gene is vraB [H]

Identifier: 94993848

GI number: 94993848

Start: 439475

End: 440569

Strand: Direct

Name: vraB [H]

Synonym: MGAS10750_Spy0452

Alternate gene names: 94993848

Gene position: 439475-440569 (Clockwise)

Preceding gene: 94993847

Following gene: 94993849

Centisome position: 22.69

GC content: 44.75

Gene sequence:

>1095_bases
ATGACCATGACGGATGTTTATATTGCTGCAGGCCTTAGAACCCCTATCGGTTTAGTAGGGAAACAATTTGCTAAAGAACA
ACCAGAAATCCTTGGAGCAAAGCTCATCAATGCTTTACAAAATAAGTATCCAGTTCCCATTGACCAAGTGATTTGTGGCA
ATGCCGTCGGAACGGGTGGTAATATTGGGCGCTTGATGACCCTGTATTCTCATTTAGGAGAATCTGTCTCTGCTTTGACG
GTTGATATGCAGTGTGCATCAGCTGGTGCAGCCTTTGCTCTAGGTTATGCTAAAATCAAGGCAGGTATGGCAAGTAACCT
TTTAGTGGGAGGGATTGAAAGTAGCTCCTTACAACCTGAATCGGTATATGCTTCAGCTGATTGGCGCCAAGGCCCTTACA
AAGTTGCCCAGTTTTCTCCGGACACCAATAGTCCATTTGCGATGGTTGAGGGGGCAGAACGAGTGGCAAGAGAACATGGC
TTTACAAAGGAGTATTTGAATCACTGGACATTGAAGAGTCATCAAAAGGCTAGTTATTGCCAGGAGCAAACCTTGTTAGT
TGACCTTATATTAGACCTGCCAGGGGCCAGTGATCAAGGCATTCGACCGCGCTTGTCAAGTAAGGTGTTGTCAAAGGTAC
CCCCTATCTTAGGAGAAGGTCACGTGATTAGTGCAGCCAATGCCTGCCTCACCCATGATGCTGCTGCCTTTTTACAGCTA
AGTAGTCAGCCTTCAGCTTTTAAACTGATTGATGTGGTAGAAGTAGCAGGAGACCCACAGCGTAGCCCTTTAATGGTGAT
TAAGGCTAGTCAAGTCCTTTTGGAAAAACATGGTCTAGGGATGGCAGATATGACAGCGATTGAATGGAACGAAGCCTTTG
CCGTTATTGATGGTCTATTTGAAACCCATTATCCAGATTTATTGGACCGCTATAATATATTTGGTGGTGCATTAGCTTAT
GGTCATCCCTACGGTGCCTCAGCAGCCATTATAATATTGCACTTAATGAGGGCTTTAGAGATCAAAAATGGACGCTATGG
AATAGCTGCTATAGCAGCAGCTGGTGGCCAAGGGTTTGCTGTTTTACTAAAATAA

Upstream 100 bases:

>100_bases
TTTTATGCGCCAGTGGTTTTAGATAGCTCTATCCAATTGATGATGAAAGGTAGAGCCATTGGCCTGTTTCAGGCTGGTAA
ATTAGTAGCTAAAGGAGATT

Downstream 100 bases:

>100_bases
TCATAAGGAGTTTAGTCATGCTGACAAAGCTAGAATATTGGGCAAAGCAATGCCCAAATAAAAAAGCTATCGTAGCCGAT
CAGATAAGCTTGACTTATCA

Product: acetyl-CoA acetyltransferase

Products: CoA; acetoacetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTMTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNAVGTGGNIGRLMTLYSHLGESVSALT
VDMQCASAGAAFALGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHG
FTKEYLNHWTLKSHQKASYCQEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQL
SSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAY
GHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLK

Sequences:

>Translated_364_residues
MTMTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNAVGTGGNIGRLMTLYSHLGESVSALT
VDMQCASAGAAFALGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHG
FTKEYLNHWTLKSHQKASYCQEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQL
SSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAY
GHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLK
>Mature_363_residues
TMTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTV
DMQCASAGAAFALGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHGF
TKEYLNHWTLKSHQKASYCQEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLS
SQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYG
HPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLK

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=27.2941176470588, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=26.0869565217391, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=27.3657289002558, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI148539872, Length=388, Percent_Identity=26.2886597938144, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=25.6345177664975, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=29.7029702970297, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87082165, Length=409, Percent_Identity=28.6063569682152, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=28.9002557544757, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=28.3582089552239, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=25, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=27.4358974358974, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=28.498727735369, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=26.3279445727483, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25147385, Length=379, Percent_Identity=25.8575197889182, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=26.8472906403941, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=27.1794871794872, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=29.4871794871795, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=27.2941176470588, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=25.765306122449, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=25.7575757575758, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9

Molecular weight: Translated: 38738; Mature: 38607

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNAVGTGG
CCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCC
NIGRLMTLYSHLGESVSALTVDMQCASAGAAFALGYAKIKAGMASNLLVGGIESSSLQPE
CHHHHHHHHHHHCCCCEEEEEEEHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHGFTKEYLNHWTLKSHQKASYC
CEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHH
QEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHEE
SSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLF
CCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHH
ETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFA
HHCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEE
VLLK
EEEC
>Mature Secondary Structure 
TMTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNAVGTGG
CCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCC
NIGRLMTLYSHLGESVSALTVDMQCASAGAAFALGYAKIKAGMASNLLVGGIESSSLQPE
CHHHHHHHHHHHCCCCEEEEEEEHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHGFTKEYLNHWTLKSHQKASYC
CEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHH
QEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHEE
SSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLF
CCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHH
ETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFA
HHCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEE
VLLK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA

Specific reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA