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The map label for this gene is nifS3 [H]

Identifier: 94993637

GI number: 94993637

Start: 251676

End: 252902

Strand: Direct

Name: nifS3 [H]

Synonym: MGAS10750_Spy0241

Alternate gene names: 94993637

Gene position: 251676-252902 (Clockwise)

Preceding gene: 94993636

Following gene: 94993638

Centisome position: 12.99

GC content: 42.87

Gene sequence:

>1227_bases
TTGCTAGACGCAAAAGACATCAAACAAGACTTTCAAATCTTAAATCAACAAGTCAATGATGAACCCCTTGTTTATTTGGA
TAATGCCGCCACCACACAAAAACCGGCGCTTGTTCTTGAGGCTTTGCAATCCTATTATCAAGAAGATAATGCTAATGTTC
ACCGAGGAGTTCATACCTTGGCTGAACGTGCAACGCACAAATATGAGGCCAGTCGCCAGCACGTTGCTGACTTTATTCAT
GCTAAATCAAGCAAGGAAGTGCTCTTTACCAGAGGAACAACAACCAGTTTGAATTGGGTTGCTCGGTTTGCAGAGCAGGT
CTTGACGCCAGAAGATGAGGTGTTGATTTCGATTATGGAGCATCATGCCAATATCATTCCTTGGCAACAAGCCTGTCAAA
AAACAGGAGCAAGGTTAGTCTATGTTTATTTAAAAGATGGCCAACTTGACATGGACGATTTGACAAACAAATTGACGACA
AAAACACGTTTTGTTAGCCTAGTACATGTCTCCAGTGTTCTTGGTTGCATCAATCCCATTAAAGAAATTGCCAAGCTGGC
ACATGCTAAAGGAGCCTACCTTGTTGTTGACGGTGCCCAGTCGGTTCCACATTTGGCTATTGATGTTCAAGACTTGGATT
GTGATTTCTTTGCTTTTTCAGCTCATAAGATGTTGGGGCCAACAGGTTTGGGTGTTCTTTACGGCAAAGAAGAGCTTTTG
AATCAAGTGGAGCCTCTTGAATTTGGCGGAGAAATGATTGATTTTGTTTACGAACAAGAGGCCACTTGGAAAGAATTGCC
CTGGAAGTTTGAAGCAGGAACACCTCACATAGCTGGTGCTATTGGGCTAAGCGCAGCCATTTCTTACCTTCAGAGACTAG
GCATGGCTGATATACATGCGCATGAAGCAGAACTAATAGCCTATGTTTTGCCGAAATTAGAAGCTATTGAGGGGCTTACC
ATATATGGACCAAGCCAGTCTAGTGCAAGATCTGGTCTGATTTCTTTTAATCTGGATGATTTGCATCCTCATGACTTGGC
AACAGCCTTGGACTATGAAGGTGTTGCAGTAAGAGCAGGGCACCACTGCGCCCAACCTCTTCTTAGTTATTTAGGTGTAC
CAGCAACTGTTAGAGCAAGTTTTTATATCTATAACACCAAGGCAGATTGTGACCGTCTAGTCGAAGCAATTCTAAAAGCA
AAGGAGTTTTTCAATGGCACTCTCTAA

Upstream 100 bases:

>100_bases
TGAAATTCCTATTCCATCAGTCCGCCAAGAGATTATTAAGGTTTTAGATGAGAAATTGCTTAATCGTTAAGACCTACTGC
CAAAAAGAAAGAGGTTAGTA

Downstream 100 bases:

>100_bases
ACTGAACCATCTATACATGGCTGTGGTAGCGGACCATTCGAAACGTCCACATCATCATGGGCAACTAGATGGCGTAGAGG
CTGTTCGACTGAATAATCCG

Product: cysteine desulfurase / Selenocysteine lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQSYYQEDNANVHRGVHTLAERATHKYEASRQHVADFIH
AKSSKEVLFTRGTTTSLNWVARFAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTT
KTRFVSLVHVSSVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFAFSAHKMLGPTGLGVLYGKEELL
NQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPHIAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLT
IYGPSQSSARSGLISFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDRLVEAILKA
KEFFNGTL

Sequences:

>Translated_408_residues
MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQSYYQEDNANVHRGVHTLAERATHKYEASRQHVADFIH
AKSSKEVLFTRGTTTSLNWVARFAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTT
KTRFVSLVHVSSVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFAFSAHKMLGPTGLGVLYGKEELL
NQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPHIAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLT
IYGPSQSSARSGLISFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDRLVEAILKA
KEFFNGTL
>Mature_408_residues
MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQSYYQEDNANVHRGVHTLAERATHKYEASRQHVADFIH
AKSSKEVLFTRGTTTSLNWVARFAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTT
KTRFVSLVHVSSVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFAFSAHKMLGPTGLGVLYGKEELL
NQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPHIAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLT
IYGPSQSSARSGLISFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDRLVEAILKA
KEFFNGTL

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=228, Percent_Identity=28.9473684210526, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1787970, Length=402, Percent_Identity=46.5174129353234, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789175, Length=386, Percent_Identity=44.559585492228, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI48994898, Length=230, Percent_Identity=27.8260869565217, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=232, Percent_Identity=31.8965517241379, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI193211090, Length=385, Percent_Identity=26.2337662337662, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319831, Length=228, Percent_Identity=29.8245614035088, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20129463, Length=241, Percent_Identity=30.2904564315353, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45186; Mature: 45186

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQSYYQEDNANVHRGVHTL
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AERATHKYEASRQHVADFIHAKSSKEVLFTRGTTTSLNWVARFAEQVLTPEDEVLISIME
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
HHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTTKTRFVSLVHVSSVLGCINPI
HCCCCCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFAFSAHKMLGPTGLGVLYGKEELL
HHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEHHHHHHCCCCCCEEEECHHHHH
NQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPHIAGAIGLSAAISYLQRLGMADIHA
HHCCCHHHCCHHHHHHHHCCCCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
HEAELIAYVLPKLEAIEGLTIYGPSQSSARSGLISFNLDDLHPHDLATALDYEGVAVRAG
HHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCEEEECCCCCCHHHHHHHCCCCCEEEECC
HHCAQPLLSYLGVPATVRASFYIYNTKADCDRLVEAILKAKEFFNGTL
HHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQSYYQEDNANVHRGVHTL
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AERATHKYEASRQHVADFIHAKSSKEVLFTRGTTTSLNWVARFAEQVLTPEDEVLISIME
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
HHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTTKTRFVSLVHVSSVLGCINPI
HCCCCCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFAFSAHKMLGPTGLGVLYGKEELL
HHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEHHHHHHCCCCCCEEEECHHHHH
NQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPHIAGAIGLSAAISYLQRLGMADIHA
HHCCCHHHCCHHHHHHHHCCCCHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
HEAELIAYVLPKLEAIEGLTIYGPSQSSARSGLISFNLDDLHPHDLATALDYEGVAVRAG
HHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCEEEECCCCCCHHHHHHHCCCCCEEEECC
HHCAQPLLSYLGVPATVRASFYIYNTKADCDRLVEAILKAKEFFNGTL
HHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]