Click here to switch to the map view.

The map label for this gene is nanH [H]

Identifier: 94993608

GI number: 94993608

Start: 222523

End: 223437

Strand: Direct

Name: nanH [H]

Synonym: MGAS10750_Spy0212

Alternate gene names: 94993608

Gene position: 222523-223437 (Clockwise)

Preceding gene: 94993607

Following gene: 94993609

Centisome position: 11.49

GC content: 43.72

Gene sequence:

>915_bases
ATGACAGATCTTACGAAATACCAAGGCATTATTCCTGCCTTTTACGCTTGTTACGATGACCAGGGGAATATCAGCCCTGA
GCGTGTGCGTGCCTTGACCCAGTACTATATTGACAAAGGGGTTCAGGGGCTTTACATTAATGGCTCTTCTGGAGAGTGTA
TTTACCAGAGCGTTTTTGACCGTCAATTGGTGTTAGAAAATGTCATGGCAGTCGCTAAGGGCAAGCTGACGATTATTAAT
CACGTTGCCTGCAACAACACTAAAGATAGTATTGAATTGGCAGCTCATTCAGAACGTTTGGGCGTGGATGCCATTGCAGC
TATTCCGCCTATTTATTTCCGGTTACCAGAATATGCAGTTGCTGATTATTGGAATGCGATTTCTTCGGCTGCTCCTCACA
CAGATTTTATCATCTATAATATTCCTCAACTAGCAGGGGTTGCCTTGACCCCAAGCCTTTATAAGACTATGTTGGCTAAT
AAGCGCGTGATTGGCGTTAAAAATTCATCTATGCCAGTGCAGGATATTCAAACCTTCTGTGCGATTGGTGGGGACGACCG
TATTGTTTTTAATGGCCCAGATGAACAATTTCTAGGAGGGCGCTTGATGGGTGCTGCTGCAGGCATTGGTGGTACCTACG
GGGCTATGCCAGAATTATTTTTAAGGCTGAACCAACTTATTGCTGACAAGGATTTAGAAAAGGCCAAGGCCCTTCAATAT
ACTATTAATGAGATTATCGGTGTCCTTGTTTCTGCTCACGGCAATATGTATGGGGTTATTAAGGAAGTACTTCGTATTAA
TGAGGGTCTTGATATTGGTTCTGTTCGATCACCTTTAGCTGAGTTGGTCGAGGAAGATAGGGTTATTTGCCAACGGGCTG
CTGCTCTGATTAATCAGGCTAAGGAAACCTTTTAA

Upstream 100 bases:

>100_bases
TCATCGGCATCAGTAGCCTAACTTACCTCTACTTGATTATCATGGAATCTCTTCTTAGGCGATTCCCACTTAATAATGAC
ATTGAATAAAGGACATTACT

Downstream 100 bases:

>100_bases
TAAGATGCGAGGGGATAACAAATGAAGCATTATTTAGCAATTGATATTGGTGGTACTGCTATCAAGTATGGCTTGATTTC
TGAAACAGGAGACCTGTTGG

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGLYINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIIN
HVACNNTKDSIELAAHSERLGVDAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
KRVIGVKNSSMPVQDIQTFCAIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPELFLRLNQLIADKDLEKAKALQY
TINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLAELVEEDRVICQRAAALINQAKETF

Sequences:

>Translated_304_residues
MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGLYINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIIN
HVACNNTKDSIELAAHSERLGVDAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
KRVIGVKNSSMPVQDIQTFCAIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPELFLRLNQLIADKDLEKAKALQY
TINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLAELVEEDRVICQRAAALINQAKETF
>Mature_303_residues
TDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGLYINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINH
VACNNTKDSIELAAHSERLGVDAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLANK
RVIGVKNSSMPVQDIQTFCAIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPELFLRLNQLIADKDLEKAKALQYT
INEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLAELVEEDRVICQRAAALINQAKETF

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=288, Percent_Identity=27.4305555555556, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789620, Length=281, Percent_Identity=32.0284697508897, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI87082415, Length=239, Percent_Identity=27.1966527196653, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786463, Length=244, Percent_Identity=25.8196721311475, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788823, Length=235, Percent_Identity=22.1276595744681, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33200; Mature: 33068

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGLYINGSSGECIYQSVFD
CCCCHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGVDAIAAIPPIYFRLPEYAV
HHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCHHHHCCCCHHH
ADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLANKRVIGVKNSSMPVQDIQTFC
HHHHHHHHCCCCCCCEEEEECHHHCCCEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPELFLRLNQLIADKDLEKAKALQY
HCCCCCEEEECCCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLAELVEEDRVICQRAAALINQA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETF
HHCC
>Mature Secondary Structure 
TDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGLYINGSSGECIYQSVFD
CCCHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGVDAIAAIPPIYFRLPEYAV
HHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCHHHHCCCCHHH
ADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLANKRVIGVKNSSMPVQDIQTFC
HHHHHHHHCCCCCCCEEEEECHHHCCCEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPELFLRLNQLIADKDLEKAKALQY
HCCCCCEEEECCCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVRSPLAELVEEDRVICQRAAALINQA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA