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The map label for this gene is istB [H]

Identifier: 94972005

GI number: 94972005

Start: 510912

End: 511712

Strand: Direct

Name: istB [H]

Synonym: Dgeo_2537

Alternate gene names: 94972005

Gene position: 510912-511712 (Clockwise)

Preceding gene: 94972006

Following gene: 94972003

Centisome position: 88.99

GC content: 63.3

Gene sequence:

>801_bases
ATGATCGCGGTCGAACGCTGCCGAACGGATCTGGAAGCGTTGGGCTTGCCGCATGCGGCAAGCCTGCTGGAGAGCCGCCT
GGACGCAGCGGCCAAGAAGGAGCTGCCCTATGCGGACTTCTTGGCCGACCTGCTGCGGATCGAGGTGGTGGCTCGGGAGG
ACGAAGGCCGTGCCCGACGGTTAAAACAGGCCCGTTTGCCTTTTATGCGGACCCTGGATCAGTTCGACTTCGCCTTCCAG
CCGAGCGTGGACAAGCGGCTGGTCAAGGAACTGGGAACGCTGTCCTTCGCCGCCGATGGGCAGAACGTCATTCTGTTGGG
GCCGCCTGGGGTCGGGAAGACCCACCTGGCTGTAGGACTCTGCACGACCGCAATCAACCACGGGGAGAGTGTGCTATTTG
TGCGGGCGGGGCAGTTGATGGAGGACCTGCGCAAAGCGCAGGCCCTGAATCGACTGGAACACCGGCTGCGGTACTACGCC
AAGCCCAAGCTGCTGGTCATCGACGAGTTCGGCGTCTGGCCCTATGACCGCCTGGCCGCCAATGCCCTGTTCGGCCTGAT
CGCGGCCAGGTACGAGCGAGGCAGCGTGATCCTGACATCGAACAAGGGCTTCGCGGATTGGGGCGAGGTGCTGGGGGACC
CGGTGGTGGCGAGTGCGATCCTGGACCGGCTGCTGCATCACAGCCACGTGTTGAACATCAAGGGTGAGTCGTATCGCCTG
CGGGAGAAGAAGAAATCCGGTCTGTTCCCCAGTGCGTTAGTGGGGATCGGCGAGGCGAGTCAGGAGGTGAAACGGCCTTG
A

Upstream 100 bases:

>100_bases
TCGAGCAGCGTTCGTTGGCGGAGTACGACGCCCTGGTCACCGTCGGCACCACCGTCACGCTGGACGAGGTGCTAGCCACC
GTGTTCCGGGAGGACGAAGC

Downstream 100 bases:

>100_bases
ACGCAGCGGGGGTGGTCAAAACCATTCCGCCGAAAATGGTCACTTCTACCGCGCCGTTGACAGTACCCCAGCCTTCACCT
GGACGTCCAGGACTTTGGGT

Product: IstB-like ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MIAVERCRTDLEALGLPHAASLLESRLDAAAKKELPYADFLADLLRIEVVAREDEGRARRLKQARLPFMRTLDQFDFAFQ
PSVDKRLVKELGTLSFAADGQNVILLGPPGVGKTHLAVGLCTTAINHGESVLFVRAGQLMEDLRKAQALNRLEHRLRYYA
KPKLLVIDEFGVWPYDRLAANALFGLIAARYERGSVILTSNKGFADWGEVLGDPVVASAILDRLLHHSHVLNIKGESYRL
REKKKSGLFPSALVGIGEASQEVKRP

Sequences:

>Translated_266_residues
MIAVERCRTDLEALGLPHAASLLESRLDAAAKKELPYADFLADLLRIEVVAREDEGRARRLKQARLPFMRTLDQFDFAFQ
PSVDKRLVKELGTLSFAADGQNVILLGPPGVGKTHLAVGLCTTAINHGESVLFVRAGQLMEDLRKAQALNRLEHRLRYYA
KPKLLVIDEFGVWPYDRLAANALFGLIAARYERGSVILTSNKGFADWGEVLGDPVVASAILDRLLHHSHVLNIKGESYRL
REKKKSGLFPSALVGIGEASQEVKRP
>Mature_266_residues
MIAVERCRTDLEALGLPHAASLLESRLDAAAKKELPYADFLADLLRIEVVAREDEGRARRLKQARLPFMRTLDQFDFAFQ
PSVDKRLVKELGTLSFAADGQNVILLGPPGVGKTHLAVGLCTTAINHGESVLFVRAGQLMEDLRKAQALNRLEHRLRYYA
KPKLLVIDEFGVWPYDRLAANALFGLIAARYERGSVILTSNKGFADWGEVLGDPVVASAILDRLLHHSHVLNIKGESYRL
REKKKSGLFPSALVGIGEASQEVKRP

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29490; Mature: 29490

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVERCRTDLEALGLPHAASLLESRLDAAAKKELPYADFLADLLRIEVVAREDEGRARR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCHHHHH
LKQARLPFMRTLDQFDFAFQPSVDKRLVKELGTLSFAADGQNVILLGPPGVGKTHLAVGL
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHH
CTTAINHGESVLFVRAGQLMEDLRKAQALNRLEHRLRYYAKPKLLVIDEFGVWPYDRLAA
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
NALFGLIAARYERGSVILTSNKGFADWGEVLGDPVVASAILDRLLHHSHVLNIKGESYRL
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCHHH
REKKKSGLFPSALVGIGEASQEVKRP
HHHHHCCCCHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MIAVERCRTDLEALGLPHAASLLESRLDAAAKKELPYADFLADLLRIEVVAREDEGRARR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCHHHHH
LKQARLPFMRTLDQFDFAFQPSVDKRLVKELGTLSFAADGQNVILLGPPGVGKTHLAVGL
HHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHH
CTTAINHGESVLFVRAGQLMEDLRKAQALNRLEHRLRYYAKPKLLVIDEFGVWPYDRLAA
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
NALFGLIAARYERGSVILTSNKGFADWGEVLGDPVVASAILDRLLHHSHVLNIKGESYRL
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCHHH
REKKKSGLFPSALVGIGEASQEVKRP
HHHHHCCCCHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]