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The map label for this gene is lacZ [H]

Identifier: 94971951

GI number: 94971951

Start: 566607

End: 568478

Strand: Direct

Name: lacZ [H]

Synonym: Dgeo_2483

Alternate gene names: 94971951

Gene position: 566607-568478 (Clockwise)

Preceding gene: 94971952

Following gene: 94971946

Centisome position: 98.69

GC content: 63.73

Gene sequence:

>1872_bases
TTGCCGCTTTCAACTCACCCCACTCCCCTGCTCGAACGTGACCACTGGCGTAGCCTAGATGGCCTCTGGCGGTTTTGCTT
TGATGACGAGGCGCGCTGGCAGCATCCCCGCGAGGTGGTCTTCGACCGCGAGATCCGGGTGCCCTATGCGCCGGAAAGCT
TAAGCAGCGGCGTCCATGACCAGGGGTTCCACTCGACCGTCTGGTACAGCCTGACTGTGACGCTCACTCCCGAGGAACGC
TCTGAACGGTTGCTGCTGCATTTCGGGGCGGTGGACTATCTGGCCACGGTGTGGGTCAATGGCCGCCTGGTCGCTCAGCA
CGAGGGGGGCAGCACGCCCTTTACTGCCGACATTACCCAGCAGGCCCAAGCCGGAGAGACGGTGGAGCTTGTGGTGCGTG
CCCAAGACAACCCCCACGACCTGGCCAAGCCGCGCGGCAAGCAAGACTGGCAACGCGAACCGCACTCAATTTGGTATCCC
CGCACAACCGGGATCTGGCAGACGGTGTGGCTGGAGCGGGTGCCTCAGACCTATCTTCGCAAGCTGCGCTGGTTGGGCGA
CATGGAGCACTGGCAGATCGGGCTGGAGGCCGAGGTGGTGGGACCGCTGCCCCCGGACCTGGGTTTGCGGGTGAGGCTCT
GGTGTAATGGCGACCTGCTGGCCGATGACCGTTACGCTGTGCGGACCACCGAGATCGCGCGGAGGATTGCCCTGGCCGAC
CCTGGGATCGACGACTTCCGCAACGAATTGTTGTGGAGTCCCGGCCATCCCCAGCTGATCGACGCCGAACTGGAATTGCT
CGTCGGAGACCGGGTGATCGACCGGGTGCGCAGCTACACGGCCTTGCGCTCGGTGAGCGTGAATGGCAACCGTTTCCTCC
TCAATGGCCGTCCCTATTATCTGCGGCTGGTGCTGGACCAAGGCTACTGGCCAGACAGCTTGATGACCGCCACCGACGAG
GAGCTGCGCCGTGACGTGGAGCTTGCCAAGCGGCTGGGGTTTAACGGGGTCCGCAAGCATCAGAAAATCGAGAGTCCGCG
CTGGCTGTACTGGTGTGACGTGCTGGGCTTGCTCGTCTGGGAGGAAATGCCCAGTGCCTACCGCTTTACCCCGGCGGCAG
TGGGGCGCCTTACCCGTGAGTGGCTCGAGGTGCTGGAACGCGACATCTCGCATCCCTGCATCGTGACCTGGGTGCCCCTC
AACGAGTCCTGGGGGGTGCCTGATCTTCCCACCAATCCGGCCCACCGCGACTACGTCCGGGCGCTGTACTTCCTCACCAA
GACCCTCGATCCCTCGCGCCCAGTGGTGGGCAACGACGGTTGGGAACACGTGGCAACGGACATCGTCACCATCCACGACT
ACGCCGACGACATTGAGCTGCTGCGGCGGCGCTACGGCACCCTGGAAGCAACTCGCCTGACGCTGCAACAGCAGCAGCCC
GCTGACCGTGCCCTCACACTGGCGGGGTTTGAAGTGGCCGGGCAGCCGGTGGTGCTGTCTGAATTTGGCGGTATTGCCTA
TATTCCCGGGCAGGAGTTGGGCTGGGGCTACAGCGAGTCGCAGAGCGAGGAGGACTTCGTCAAGGACTACGGCGCGCTCC
TCTCGGCCATCCACGAGTGTCACGGCTTATCGGGGTTCTGCTACACCCAGCTCACCGACACCTTTCAGGAGAAAAACGGC
CTTCTGTACGCCGACCGCACGCCCAAGGCCGACATCCAGAAACTCGCGCGCAGCACCCAGGGTACCCGCACCGCCCGCGA
GATGACGGTCGATCCCATCCTTAACCCCTTCGGCAACTCGGCGCGTTGGCTGCGGCGCCAGCAGGAAACCCTGATGTTCA
TCGAGATGAATCTGGACGTGGACCGTGAGTGA

Upstream 100 bases:

>100_bases
CGGGAACGCGGAAGCGGAAAGGCGGAGGGTCTATCATGTTCCGGTTCTTTCCCGTGTGACCTCCCGGACTTGACCCACCG
CCCCAGGCGAAGGAGACATC

Downstream 100 bases:

>100_bases
GCAGGAAGGCGTGGCCGCTCTGCCCGAACCAACGCTTTCTCGCTCTCTCCTCTCGCACAGGCCAACCGGGCGTGCTCCCT
AGCGCCGGGCCAGCCGCCCC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MPLSTHPTPLLERDHWRSLDGLWRFCFDDEARWQHPREVVFDREIRVPYAPESLSSGVHDQGFHSTVWYSLTVTLTPEER
SERLLLHFGAVDYLATVWVNGRLVAQHEGGSTPFTADITQQAQAGETVELVVRAQDNPHDLAKPRGKQDWQREPHSIWYP
RTTGIWQTVWLERVPQTYLRKLRWLGDMEHWQIGLEAEVVGPLPPDLGLRVRLWCNGDLLADDRYAVRTTEIARRIALAD
PGIDDFRNELLWSPGHPQLIDAELELLVGDRVIDRVRSYTALRSVSVNGNRFLLNGRPYYLRLVLDQGYWPDSLMTATDE
ELRRDVELAKRLGFNGVRKHQKIESPRWLYWCDVLGLLVWEEMPSAYRFTPAAVGRLTREWLEVLERDISHPCIVTWVPL
NESWGVPDLPTNPAHRDYVRALYFLTKTLDPSRPVVGNDGWEHVATDIVTIHDYADDIELLRRRYGTLEATRLTLQQQQP
ADRALTLAGFEVAGQPVVLSEFGGIAYIPGQELGWGYSESQSEEDFVKDYGALLSAIHECHGLSGFCYTQLTDTFQEKNG
LLYADRTPKADIQKLARSTQGTRTAREMTVDPILNPFGNSARWLRRQQETLMFIEMNLDVDRE

Sequences:

>Translated_623_residues
MPLSTHPTPLLERDHWRSLDGLWRFCFDDEARWQHPREVVFDREIRVPYAPESLSSGVHDQGFHSTVWYSLTVTLTPEER
SERLLLHFGAVDYLATVWVNGRLVAQHEGGSTPFTADITQQAQAGETVELVVRAQDNPHDLAKPRGKQDWQREPHSIWYP
RTTGIWQTVWLERVPQTYLRKLRWLGDMEHWQIGLEAEVVGPLPPDLGLRVRLWCNGDLLADDRYAVRTTEIARRIALAD
PGIDDFRNELLWSPGHPQLIDAELELLVGDRVIDRVRSYTALRSVSVNGNRFLLNGRPYYLRLVLDQGYWPDSLMTATDE
ELRRDVELAKRLGFNGVRKHQKIESPRWLYWCDVLGLLVWEEMPSAYRFTPAAVGRLTREWLEVLERDISHPCIVTWVPL
NESWGVPDLPTNPAHRDYVRALYFLTKTLDPSRPVVGNDGWEHVATDIVTIHDYADDIELLRRRYGTLEATRLTLQQQQP
ADRALTLAGFEVAGQPVVLSEFGGIAYIPGQELGWGYSESQSEEDFVKDYGALLSAIHECHGLSGFCYTQLTDTFQEKNG
LLYADRTPKADIQKLARSTQGTRTAREMTVDPILNPFGNSARWLRRQQETLMFIEMNLDVDRE
>Mature_622_residues
PLSTHPTPLLERDHWRSLDGLWRFCFDDEARWQHPREVVFDREIRVPYAPESLSSGVHDQGFHSTVWYSLTVTLTPEERS
ERLLLHFGAVDYLATVWVNGRLVAQHEGGSTPFTADITQQAQAGETVELVVRAQDNPHDLAKPRGKQDWQREPHSIWYPR
TTGIWQTVWLERVPQTYLRKLRWLGDMEHWQIGLEAEVVGPLPPDLGLRVRLWCNGDLLADDRYAVRTTEIARRIALADP
GIDDFRNELLWSPGHPQLIDAELELLVGDRVIDRVRSYTALRSVSVNGNRFLLNGRPYYLRLVLDQGYWPDSLMTATDEE
LRRDVELAKRLGFNGVRKHQKIESPRWLYWCDVLGLLVWEEMPSAYRFTPAAVGRLTREWLEVLERDISHPCIVTWVPLN
ESWGVPDLPTNPAHRDYVRALYFLTKTLDPSRPVVGNDGWEHVATDIVTIHDYADDIELLRRRYGTLEATRLTLQQQQPA
DRALTLAGFEVAGQPVVLSEFGGIAYIPGQELGWGYSESQSEEDFVKDYGALLSAIHECHGLSGFCYTQLTDTFQEKNGL
LYADRTPKADIQKLARSTQGTRTAREMTVDPILNPFGNSARWLRRQQETLMFIEMNLDVDRE

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787903, Length=535, Percent_Identity=26.1682242990654, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1786539, Length=439, Percent_Identity=25.9681093394077, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI62471735, Length=525, Percent_Identity=24, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922582, Length=525, Percent_Identity=24, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655438, Length=525, Percent_Identity=24, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24651745, Length=533, Percent_Identity=25.515947467167, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 71682; Mature: 71550

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSTHPTPLLERDHWRSLDGLWRFCFDDEARWQHPREVVFDREIRVPYAPESLSSGVHD
CCCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHCCCCHHHHCCCCCCCCCCCHHHHCCCCC
QGFHSTVWYSLTVTLTPEERSERLLLHFGAVDYLATVWVNGRLVAQHEGGSTPFTADITQ
CCCCEEEEEEEEEEECCCCCCCEEEEEECHHHHHHHHEECCEEEEEECCCCCCEEHHHHH
QAQAGETVELVVRAQDNPHDLAKPRGKQDWQREPHSIWYPRTTGIWQTVWLERVPQTYLR
HHCCCCEEEEEEEECCCCHHHCCCCCCCHHHCCCCCEECCCCCCHHHHHHHHHCCHHHHH
KLRWLGDMEHWQIGLEAEVVGPLPPDLGLRVRLWCNGDLLADDRYAVRTTEIARRIALAD
HHHHHCCCCCEEECCEEEEECCCCCCCCCEEEEEECCCEEECCCEEEHHHHHHHHHHHCC
PGIDDFRNELLWSPGHPQLIDAELELLVGDRVIDRVRSYTALRSVSVNGNRFLLNGRPYY
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCEE
LRLVLDQGYWPDSLMTATDEELRRDVELAKRLGFNGVRKHQKIESPRWLYWCDVLGLLVW
EEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHH
EEMPSAYRFTPAAVGRLTREWLEVLERDISHPCIVTWVPLNESWGVPDLPTNPAHRDYVR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
ALYFLTKTLDPSRPVVGNDGWEHVATDIVTIHDYADDIELLRRRYGTLEATRLTLQQQQP
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCC
ADRALTLAGFEVAGQPVVLSEFGGIAYIPGQELGWGYSESQSEEDFVKDYGALLSAIHEC
CCCEEEEECCEECCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HGLSGFCYTQLTDTFQEKNGLLYADRTPKADIQKLARSTQGTRTAREMTVDPILNPFGNS
CCCCCEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
ARWLRRQQETLMFIEMNLDVDRE
HHHHHHHCCEEEEEEECCCCCCC
>Mature Secondary Structure 
PLSTHPTPLLERDHWRSLDGLWRFCFDDEARWQHPREVVFDREIRVPYAPESLSSGVHD
CCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHCCCCHHHHCCCCCCCCCCCHHHHCCCCC
QGFHSTVWYSLTVTLTPEERSERLLLHFGAVDYLATVWVNGRLVAQHEGGSTPFTADITQ
CCCCEEEEEEEEEEECCCCCCCEEEEEECHHHHHHHHEECCEEEEEECCCCCCEEHHHHH
QAQAGETVELVVRAQDNPHDLAKPRGKQDWQREPHSIWYPRTTGIWQTVWLERVPQTYLR
HHCCCCEEEEEEEECCCCHHHCCCCCCCHHHCCCCCEECCCCCCHHHHHHHHHCCHHHHH
KLRWLGDMEHWQIGLEAEVVGPLPPDLGLRVRLWCNGDLLADDRYAVRTTEIARRIALAD
HHHHHCCCCCEEECCEEEEECCCCCCCCCEEEEEECCCEEECCCEEEHHHHHHHHHHHCC
PGIDDFRNELLWSPGHPQLIDAELELLVGDRVIDRVRSYTALRSVSVNGNRFLLNGRPYY
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCEE
LRLVLDQGYWPDSLMTATDEELRRDVELAKRLGFNGVRKHQKIESPRWLYWCDVLGLLVW
EEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHH
EEMPSAYRFTPAAVGRLTREWLEVLERDISHPCIVTWVPLNESWGVPDLPTNPAHRDYVR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
ALYFLTKTLDPSRPVVGNDGWEHVATDIVTIHDYADDIELLRRRYGTLEATRLTLQQQQP
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCC
ADRALTLAGFEVAGQPVVLSEFGGIAYIPGQELGWGYSESQSEEDFVKDYGALLSAIHEC
CCCEEEEECCEECCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HGLSGFCYTQLTDTFQEKNGLLYADRTPKADIQKLARSTQGTRTAREMTVDPILNPFGNS
CCCCCEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
ARWLRRQQETLMFIEMNLDVDRE
HHHHHHHCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA