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The map label for this gene is argG [H]

Identifier: 94314845

GI number: 94314845

Start: 126776

End: 128101

Strand: Direct

Name: argG [H]

Synonym: Rmet_5926

Alternate gene names: 94314845

Gene position: 126776-128101 (Clockwise)

Preceding gene: 94314841

Following gene: 94314846

Centisome position: 4.91

GC content: 64.03

Gene sequence:

>1326_bases
ATGACTACCATCCTGCAGCACATTCCTTCCGGCCAGCGCGTCGGAATCGCCTTCTCGGGCGGACTCGATACCAGCGCCGC
GCTCCTCTGGATGCGCCAGAAGGGCGCCATTCCCTACGCCTACACGGCGAACCTGGGCCAGCCCGACGAACCGGATTACG
ACGACATCCCCCGCCGCGCCATGGCGTACGGCGCCGAGGCCGCCCGTCTGGTGGATTGCCGCACCCAACTGGTGGCCGAA
GGTATCGCCGCGCTGCAATGCGGCGCGTTCCATATCTCGACCGCCGGCGTGACCTACTTCAACACGACCCCGATCGGCCG
TGCCGTGACCGGCACGATGCTGGTGGCGGCCATGAAGGAAGACGGCGTCAACATCTGGGGCGACGGCAGCACGTTCAAGG
GCAACGACATCGAGCGCTTCTACCGCTACGGCCTGCTGACCAACCCGGGTCTGCAGATCTACAAGCCGTGGCTGGACCAG
CAGTTCATCGACGAACTGGGTGGCCGCGCCGAAATGTCCGAGTTCATGCGCCAGCACGGCCATGCCTACAAGATGTCGGC
GGAAAAGGCGTACTCGACCGATTCGAACATGCTCGGCGCCACGCACGAGGCCAAGGATCTGGAGCACCTGGATTCCGGCA
TCCGCATCGTCCAGCCGATCATGGGCGTGCAGTTCTGGCGCGATGACGTCGAAGTGAAGCGCGAGGAAGTGACCGTGCGC
TTCGAGGAAGGCCAGCCGGTGGCGCTGAATGGCAAGACGTTCGCCAATGCCGTCGACCTGCTCATGGAAGCCAATGTCAT
CGGTGGCCGTCATGGCCTGGGCATGAGCGACCAGATCGAGAACCGCATCATCGAAGCCAAGAGCCGCGGCATCTACGAAG
CCCCGGGCCTGGCGCTGCTGTTCATCGCTTACGAGCGCCTGATCACCGGTATCCACAACGAAGACACCATCGAGCAGTAC
CGCGACAACGGCCGCCGTCTGGGCCGCCTGCTGTACCAGGGCCGCTGGTTCGATCCGCAAGCCATCATGCTGCGCGAAAC
CGCCCAGCGCTGGGTGGCTGGCGCCGTGACCGGCGAGGTGACGATCGAACTGCGTCGCGGTAACGACTACTCGATCCTGA
ACACGACGTCGCCGAACCTGACGTACAAGCCCGAGCGCCTGACGATGGAAAAGGGCGAGTCGATGTTCACGCCGCTGGAC
CGTATTGGCCAACTCACGATGCGTACGCTCGACATCGTCGACACGCGCGAGAAGCTGCAGACGTACGCCAAGTCCGGCCT
GCTGTCGAACCAGACCAGCGCGGCGCTGCCAAAGCTCGAAGACTGA

Upstream 100 bases:

>100_bases
AATTCACGGTTACGAGTTTCGGGCGCCGGACCGGCTCGCCGCATGACGCGATCGTGCGGGGGCGCCCCAAGAGCCGACAA
ATTCACCTGGTTTTCTGGCG

Downstream 100 bases:

>100_bases
GCAAGGCACGTTTCCGGACGGAAAAGCGGCACGAGCGATCGTGCCGCTTTTTTTTCGCCGCCGCCCGGCAGACATCTGGC
GCCCTGCAAGCTCTACCGCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTTILQHIPSGQRVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAMAYGAEAARLVDCRTQLVAE
GIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQ
QFIDELGGRAEMSEFMRQHGHAYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDDVEVKREEVTVR
FEEGQPVALNGKTFANAVDLLMEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLD
RIGQLTMRTLDIVDTREKLQTYAKSGLLSNQTSAALPKLED

Sequences:

>Translated_441_residues
MTTILQHIPSGQRVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAMAYGAEAARLVDCRTQLVAE
GIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQ
QFIDELGGRAEMSEFMRQHGHAYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDDVEVKREEVTVR
FEEGQPVALNGKTFANAVDLLMEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLD
RIGQLTMRTLDIVDTREKLQTYAKSGLLSNQTSAALPKLED
>Mature_440_residues
TTILQHIPSGQRVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAMAYGAEAARLVDCRTQLVAEG
IAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQ
FIDELGGRAEMSEFMRQHGHAYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDDVEVKREEVTVRF
EEGQPVALNGKTFANAVDLLMEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQYR
DNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDR
IGQLTMRTLDIVDTREKLQTYAKSGLLSNQTSAALPKLED

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=405, Percent_Identity=27.9012345679012, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI16950633, Length=405, Percent_Identity=27.9012345679012, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1789563, Length=441, Percent_Identity=79.5918367346939, Blast_Score=745, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=420, Percent_Identity=28.8095238095238, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI21358151, Length=421, Percent_Identity=27.3159144893112, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49157; Mature: 49026

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILQHIPSGQRVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRA
CCCHHHHCCCCCEEEEEEECCCCCHHHHEEEHHCCCCCEEEECCCCCCCCCCHHHHHHHH
MAYGAEAARLVDCRTQLVAEGIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQFIDELGGRAEMSEFMRQHG
CCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEHHHCCHHHHHHHCCHHHHHHHHHHCC
HAYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDDVEVKREEVTVR
CEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
FEEGQPVALNGKTFANAVDLLMEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
ECCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLITGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
TIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDRIGQLTMRTLDIVDTREKLQ
EEEEECCCCEEEEECCCCCCEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYAKSGLLSNQTSAALPKLED
HHHHHCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TTILQHIPSGQRVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRA
CCHHHHCCCCCEEEEEEECCCCCHHHHEEEHHCCCCCEEEECCCCCCCCCCHHHHHHHH
MAYGAEAARLVDCRTQLVAEGIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQFIDELGGRAEMSEFMRQHG
CCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEHHHCCHHHHHHHCCHHHHHHHHHHCC
HAYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDDVEVKREEVTVR
CEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
FEEGQPVALNGKTFANAVDLLMEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
ECCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLITGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
TIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDRIGQLTMRTLDIVDTREKLQ
EEEEECCCCEEEEECCCCCCEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYAKSGLLSNQTSAALPKLED
HHHHHCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]