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The map label for this gene is ynfL [H]

Identifier: 94314811

GI number: 94314811

Start: 90310

End: 91203

Strand: Reverse

Name: ynfL [H]

Synonym: Rmet_5892

Alternate gene names: 94314811

Gene position: 91203-90310 (Counterclockwise)

Preceding gene: 94314815

Following gene: 94314810

Centisome position: 3.53

GC content: 64.77

Gene sequence:

>894_bases
ATGGAATTCCGACATCTGCGCTACTTCCTTGTGCTCGCCGAGGAACTTCATTTCGGGCGCGCCGCACGGCGACTGTCGAT
TTCGCAGCCGCCGCTATCGCTCAATATCCAGCAACTCGAAGCCTCGGTCGGCGCCCGGCTGTTCGAGCGTGACAGCCGTG
GCGTGCGCCTGACCGCGGCCGGACGCGCGTTTCGGGAATCGGCCATGGCACTACTGGCGCAGGCCGAGGAAGCCCGGCTA
CTGGCGCGGGAGATCGAGGCCGGCGCGGTGGGACGGCTGCGGGTCGGCTTCGTGGGGTCGATGCTCTATCGTGGGCTGCC
CCAGCGCCTGAAGGAGTTCCAGGCGCGCTATCCCGGCATCCACGTGGCCCTGACAGAGCTGAACTCACAAGAGCAGATTG
ATGCACTTTTGCACGGTGAACTCGATGCGGGCTTTGTCCACACCAGCCGCGTGCCGGATGAGCTGGCCACCACGCTGGCG
CACCGCGAACCGTTCGTCTGCTGCATGCCAGCCAAGCATCCGCTTGGCCAACAGAATACGGTAGCGCTCACTGACTTACG
CGGCGAGCCATTCGTCCTGTTCTCCCGCAAGGCGTCGCCCGACTACTACAGCCGCATTTTCGATATGTGCGCCGCGCACG
GCTTCTATCCCCAGATTCGCCATGAGGTGCGGCACTGGCTGTCCGTGGTGTCGTTGGTGTCACAGGGGATGGGGATTGCC
GTGGTGCCAGCTGCGATGGCACGCTCCGGCATGGCAGGCGCCGCGTTTCGGCCACTTGCGGATGCGACCGTGCCTTCGGA
GGTGTATTGCGTGTGGAAGCAGGCGCCGGATCACCCGGCACGCGACCATTTCGTCGAAATGGTCAGGCTGGCCGCCAGCG
TGGCCGACATCTAG

Upstream 100 bases:

>100_bases
GGGCGGCTGTCGGGCGGGATGGTTTGCGTCCATTATTCAAACCCCAACGATTCGTGTCTAATTCTAAAATTGGCTCGATC
AATACAAATTTTGTATCGCC

Downstream 100 bases:

>100_bases
ACACTCGGCAAGAAGGCGCCAGGAATACGCCAAAAGAAGAAGCCACTGGCCGAAGCCAGTGGCTTGAGAAGTACGCGTGG
AACGGGGTGTTCAGCGTGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MEFRHLRYFLVLAEELHFGRAARRLSISQPPLSLNIQQLEASVGARLFERDSRGVRLTAAGRAFRESAMALLAQAEEARL
LAREIEAGAVGRLRVGFVGSMLYRGLPQRLKEFQARYPGIHVALTELNSQEQIDALLHGELDAGFVHTSRVPDELATTLA
HREPFVCCMPAKHPLGQQNTVALTDLRGEPFVLFSRKASPDYYSRIFDMCAAHGFYPQIRHEVRHWLSVVSLVSQGMGIA
VVPAAMARSGMAGAAFRPLADATVPSEVYCVWKQAPDHPARDHFVEMVRLAASVADI

Sequences:

>Translated_297_residues
MEFRHLRYFLVLAEELHFGRAARRLSISQPPLSLNIQQLEASVGARLFERDSRGVRLTAAGRAFRESAMALLAQAEEARL
LAREIEAGAVGRLRVGFVGSMLYRGLPQRLKEFQARYPGIHVALTELNSQEQIDALLHGELDAGFVHTSRVPDELATTLA
HREPFVCCMPAKHPLGQQNTVALTDLRGEPFVLFSRKASPDYYSRIFDMCAAHGFYPQIRHEVRHWLSVVSLVSQGMGIA
VVPAAMARSGMAGAAFRPLADATVPSEVYCVWKQAPDHPARDHFVEMVRLAASVADI
>Mature_297_residues
MEFRHLRYFLVLAEELHFGRAARRLSISQPPLSLNIQQLEASVGARLFERDSRGVRLTAAGRAFRESAMALLAQAEEARL
LAREIEAGAVGRLRVGFVGSMLYRGLPQRLKEFQARYPGIHVALTELNSQEQIDALLHGELDAGFVHTSRVPDELATTLA
HREPFVCCMPAKHPLGQQNTVALTDLRGEPFVLFSRKASPDYYSRIFDMCAAHGFYPQIRHEVRHWLSVVSLVSQGMGIA
VVPAAMARSGMAGAAFRPLADATVPSEVYCVWKQAPDHPARDHFVEMVRLAASVADI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=38.1118881118881, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI2367136, Length=297, Percent_Identity=35.3535353535354, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788887, Length=302, Percent_Identity=35.4304635761589, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=31.0606060606061, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693105, Length=264, Percent_Identity=26.8939393939394, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=29.7959183673469, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=25.4098360655738, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787806, Length=296, Percent_Identity=24.6621621621622, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=26.8292682926829, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787601, Length=256, Percent_Identity=25, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=273, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=238, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789204, Length=253, Percent_Identity=26.8774703557312, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=31.2925170068027, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=127, Percent_Identity=34.6456692913386, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32938; Mature: 32938

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRHLRYFLVLAEELHFGRAARRLSISQPPLSLNIQQLEASVGARLFERDSRGVRLTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAFRESAMALLAQAEEARLLAREIEAGAVGRLRVGFVGSMLYRGLPQRLKEFQARYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HVALTELNSQEQIDALLHGELDAGFVHTSRVPDELATTLAHREPFVCCMPAKHPLGQQNT
EEEEECCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCC
VALTDLRGEPFVLFSRKASPDYYSRIFDMCAAHGFYPQIRHEVRHWLSVVSLVSQGMGIA
EEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VVPAAMARSGMAGAAFRPLADATVPSEVYCVWKQAPDHPARDHFVEMVRLAASVADI
EECHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFRHLRYFLVLAEELHFGRAARRLSISQPPLSLNIQQLEASVGARLFERDSRGVRLTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAFRESAMALLAQAEEARLLAREIEAGAVGRLRVGFVGSMLYRGLPQRLKEFQARYPGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HVALTELNSQEQIDALLHGELDAGFVHTSRVPDELATTLAHREPFVCCMPAKHPLGQQNT
EEEEECCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCC
VALTDLRGEPFVLFSRKASPDYYSRIFDMCAAHGFYPQIRHEVRHWLSVVSLVSQGMGIA
EEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VVPAAMARSGMAGAAFRPLADATVPSEVYCVWKQAPDHPARDHFVEMVRLAASVADI
EECHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]