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The map label for this gene is glcA [H]

Identifier: 94314815

GI number: 94314815

Start: 95489

End: 97189

Strand: Reverse

Name: glcA [H]

Synonym: Rmet_5896

Alternate gene names: 94314815

Gene position: 97189-95489 (Counterclockwise)

Preceding gene: 94314816

Following gene: 94314811

Centisome position: 3.77

GC content: 66.31

Gene sequence:

>1701_bases
ATGCAACCCTGGACCCAGATCTATACGCCGCTGGGCAGCCTCTGGCTGTCCGCGCTCGCGGCGGCGATACCCATCCTGTT
TTTCTTCGTCGCACTCGCGGTACTGCGCATGAAGGGCCATGTGGCGGCGGCCGTCACGCTGGCACTGGCGCTGGCTGTGG
CGATCTTCGCGTACGGGATGCCCGCGCAGCAGGCATTTGCCGCGGCCGGCTTCGGCTTTGCCTATGGCCTGTGGCCAATC
GCGTGGATCATCGTCACCGCGGTGTTCCTCTACAAGATCGTGGTCAAGACCGGGCAGTTCGACGTCATCCGCGCGTCGGT
GCTGTCGATCACCGATGACCAGCGCCTGCAGATGCTGCTGATCGGCTTTGCCTTCGGGGCGTTCCTGGAAGGTGCGGCGG
GCTTCGGCGCACCGGTGGCGATTACCGCCGCGCTGCTGGTCGGGCTCGGGTTCAACCCGCTCTATGCGGCCGGGCTCTGC
CTGATCGCGAACACCGCGCCGGTGGCGTTTGGCGCGATGGGCATTCCGATCATCGTGGCCGGACAGGTCACGGGCATCGA
CCCGATGCATATCGGCGCGATGGCGGGCCGCCAATTGCCGCTGCTGTCGCTGGCCGTGCCGTTCTGGCTGGTGTTCATGA
TGGATGGCGCCAAGGGCGTGCGCGAGACCTGGCCCGCTGCGCTGGTCGTCGGTGGCAGCTTCGCCGTCACGCAGTACTTC
ACGTCGAACCACATCGGGCCCGAGCTGCCGGACATCACGTCGGCACTGGTCAGTCTGATCTCGCTCGCCGCGTTCCTGCG
TGTGTGGCAACCGCGCTCGGCATCGCAAGCGGCTGGTGGCACGGTTGCGGCCGGCGGCACTGTGGCGATGGCCGGGTTCG
GTGGCGGTATGGGTGCGCCCGCGAGCCGCAAAGCATCGCCCTACACGTTCGGCCAGACCGTGCGCGCATGGGCGCCGTTC
GGCATCCTGACCGCCATCGTCACGGTCTGGAGCCTGGCACCGTTCAAGGCACTGTTCGCCGGCAACGGCGCGCTGGCGGG
AACGGTACTGAAGTTCAAGGTGCCGATGCTCGACCAGCTCGTGATCAAGAGCGCCCCGATCGTGGCATCGCCCAAGCCCT
ATGAGGCCGTGCTGAAGCTCGACCTGCTGTCGGCCGTCGGTACCGCGATCCTGCTGACCGCCATCATCTCGGCAGTGCTG
CTGCGCATGAAGCCGAGCGATGCCATCACCACCTTCAAGGAAACGCTGCTGGAACTGCGCCGCCCGATCGCCTCGATCGG
TCTGGTACTGGCGTTCGCGTTCGTCGCCAACTACTCCGGTATGTCATCGACGCTGGCGCTGCTGCTGGCCGGAACCGGCG
CGGCGTTCCCGTTCTTCTCGCCGCTGCTGGGCTGGCTGGGGGTGTTCCTGACCGGCTCGGATACGTCGTCGAACGCGCTG
TTCTGCTCGCTGCAGCATACGACCGCGCATCAGATCGGCGTATCGGACACGCTGCTGGTGGCCGCCAACACCACGGGCGG
CGTGACCGGCAAGATGATCTCGCCGCAATCGATCGCGGTGGCTTGCGCGGCGACGGGTCTGGTCGGCAAGGAGTCAGAAC
TGTTCCGCTTCACCGTGAAGCACAGCCTGCTGTTCGCGGCGATCGTGGGTGTGATCACGATGATCCAGGCGTACTGGCTG
ACGGGGATGATTCCGGGGTAG

Upstream 100 bases:

>100_bases
AGCCTTTCCGACCTGCTCGACGCACGCAACCAGCCCGAGTAGCGGCATCGCGCGGCATCCCACCAAAAGAACTCAGACAA
ACCACCAAGGAGACTTCCAG

Downstream 100 bases:

>100_bases
GAAGCGGTGAGGGTGTTTTTGCCCCCCCTCTCCCGCCGGGAGAGGGGGGCGCATACCAACGGTTGGCACCCAACTCAATC
GTTCGCCGCCTCGCGCGGCA

Product: glycolate transporter

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MQPWTQIYTPLGSLWLSALAAAIPILFFFVALAVLRMKGHVAAAVTLALALAVAIFAYGMPAQQAFAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDPMHIGAMAGRQLPLLSLAVPFWLVFMMDGAKGVRETWPAALVVGGSFAVTQYF
TSNHIGPELPDITSALVSLISLAAFLRVWQPRSASQAAGGTVAAGGTVAMAGFGGGMGAPASRKASPYTFGQTVRAWAPF
GILTAIVTVWSLAPFKALFAGNGALAGTVLKFKVPMLDQLVIKSAPIVASPKPYEAVLKLDLLSAVGTAILLTAIISAVL
LRMKPSDAITTFKETLLELRRPIASIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPLLGWLGVFLTGSDTSSNAL
FCSLQHTTAHQIGVSDTLLVAANTTGGVTGKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAAIVGVITMIQAYWL
TGMIPG

Sequences:

>Translated_566_residues
MQPWTQIYTPLGSLWLSALAAAIPILFFFVALAVLRMKGHVAAAVTLALALAVAIFAYGMPAQQAFAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDPMHIGAMAGRQLPLLSLAVPFWLVFMMDGAKGVRETWPAALVVGGSFAVTQYF
TSNHIGPELPDITSALVSLISLAAFLRVWQPRSASQAAGGTVAAGGTVAMAGFGGGMGAPASRKASPYTFGQTVRAWAPF
GILTAIVTVWSLAPFKALFAGNGALAGTVLKFKVPMLDQLVIKSAPIVASPKPYEAVLKLDLLSAVGTAILLTAIISAVL
LRMKPSDAITTFKETLLELRRPIASIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPLLGWLGVFLTGSDTSSNAL
FCSLQHTTAHQIGVSDTLLVAANTTGGVTGKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAAIVGVITMIQAYWL
TGMIPG
>Mature_566_residues
MQPWTQIYTPLGSLWLSALAAAIPILFFFVALAVLRMKGHVAAAVTLALALAVAIFAYGMPAQQAFAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDPMHIGAMAGRQLPLLSLAVPFWLVFMMDGAKGVRETWPAALVVGGSFAVTQYF
TSNHIGPELPDITSALVSLISLAAFLRVWQPRSASQAAGGTVAAGGTVAMAGFGGGMGAPASRKASPYTFGQTVRAWAPF
GILTAIVTVWSLAPFKALFAGNGALAGTVLKFKVPMLDQLVIKSAPIVASPKPYEAVLKLDLLSAVGTAILLTAIISAVL
LRMKPSDAITTFKETLLELRRPIASIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPLLGWLGVFLTGSDTSSNAL
FCSLQHTTAHQIGVSDTLLVAANTTGGVTGKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAAIVGVITMIQAYWL
TGMIPG

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=564, Percent_Identity=70.5673758865248, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=566, Percent_Identity=66.0777385159011, Blast_Score=696, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58665; Mature: 58665

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]